Chemical or natural tools to control microbiological contamination of surfaces

Food safety is dependent on the control of microbiological contamination on equipment surfaces in food processing facilities. Cleaning/disinfection procedures are not always adequate and microbial contamination persists as a biofilm. The microorganisms present in a biofilm are generally less sensitive to disinfection treatments than their planktonic counterparts. The increased resistance of biofilms is frequently attributed to the presence of a “protective” organic matrix and the particular physiology of cells within the biofilm. New curative methods are currently emerging for equipment surface decontamination: for instance, some of these use the interesting bactericidal potential of natural compounds.La qualité hygiénique des aliments nécessite une maîtrise de la contamination surfacique des équipements des ateliers de transformation alimentaire. Parfois, les procédures de nettoyage/désinfection s'avèrent limitées et une contamination microbienne subsiste alors sous forme de biofilm. Les micro-organismes contenus dans un biofilm sont généralement moins sensibles aux traitements de désinfection que leurs homologues planctoniques. La présence d'une matrice organique « protectrice » et la physiologie particulière des cellules au sein du biofilm sont fréquemment avancées comme explications à la résistance accrue des biofilms. De nouvelles méthodes curatives se développent actuellement pour limiter la contamination des surfaces utilisant les potentialités bactéricides intéressantes de certains antimicrobiens naturels

Positive role of cell wall anchored proteinase PrtP in adhesion of lactococci

<p>Abstract</p> <p>Background</p> <p>The first step in biofilm formation is bacterial attachment to solid surfaces, which is dependent on the cell surface physico-chemical properties. Cell wall anchored proteins (CWAP) are among the known adhesins that confer the adhesive properties to pathogenic Gram-positive bacteria. To investigate the role of CWAP of non-pathogen Gram-positive bacteria in the initial steps of biofilm formation, we evaluated the physico-chemical properties and adhesion to solid surfaces of <it>Lactococcus lactis</it>. To be able to grow in milk this dairy bacterium expresses a cell wall anchored proteinase PrtP for breakdown of milk caseins.</p> <p>Results</p> <p>The influence of the anchored cell wall proteinase PrtP on microbial surface physico-chemical properties, and consequently on adhesion, was evaluated using lactococci carrying different alleles of <it>prtP</it>. The presence of cell wall anchored proteinase on the surface of lactococcal cells resulted in an increased affinity to solvents with different physico-chemical properties (apolar and Lewis acid-base solvents). These properties were observed regardless of whether the PrtP variant was biologically active or not, and were not observed in strains without PrtP. Anchored PrtP displayed a significant increase in cell adhesion to solid glass and tetrafluoroethylene surfaces.</p> <p>Conclusion</p> <p>Obtained results indicate that exposure of an anchored cell wall proteinase PrtP, and not its proteolytic activity, is responsible for greater cell hydrophobicity and adhesion. The increased bacterial affinity to polar and apolar solvents indicated that exposure of PrtP on lactococcal cell surface could enhance the capacity to exchange attractive van der Waals interactions, and consequently increase their adhesion to different types of solid surfaces and solvents.</p

High intraspecific variation of the cell surface physico-chemical and bioadhesion properties in Brettanomyces bruxellensis

Brettanomyces bruxellensis is the most damaging spoilage yeast in the wine industry because of its negative impact on the wine organoleptic qualities. The strain persistence in cellars over several years associated with recurrent wine contamination suggest specific properties to persist and survive in the environment through bioadhesion phenomena. In this work, the physico-chemical surface properties, morphology and ability to adhere to stainless steel were studied both on synthetic medium and on wine. More than 50 strains representative of the genetic diversity of the species were considered. Microscopy techniques made it possible to highlight a high morphological diversity of the cells with the presence of pseudohyphae forms for some genetic groups. Analysis of the physico-chemical properties of the cell surface reveals contrasting behaviors: most of the strains display a negative surface charge and hydrophilic behavior while the Beer 1 genetic group has a hydrophobic behavior. All strains showed bioadhesion abilities on stainless steel after only 3 h with differences in the concentration of bioadhered cells ranging from 2.2 × 102 cell/cm2 to 7.6 × 106 cell/cm2. Finally, our results show high variability of the bioadhesion properties, the first step in the biofilm formation, according to the genetic group with the most marked bioadhesion capacity for the beer group. © 2023 The Author

Les planches d'affinage en bois de l'AOC Reblochon de Savoie (écologie microbienne et effet inhibiteur envers Listeria monocytogenes)

MASSY-AgroParisTech (913772301) / SudocSudocFranceF

ENVIRONNEMENT DES BACTERIES ET SENSIBILITE AUX BIOCIDES (MISE AU POINT D'UNE TECHNIQUE RAPIDE POUR DETERMINER IN SITU L'EFFICACAITE BACTERICIDE D'AGENTS ANTIMICROBIENS)

MASSY-AgroParisTech (913772301) / SudocSudocFranceF

INFLUENCE DE BIOSURFACTANTS SUR L'ADHESION DE LISTERIA MONOCYTOGENES A DES SURFACES INERTES (CONSEQUENCES SUR LA DESINFECTION (DOCTORAT : MICROBIOLOGIE))

CHATENAY M.-PARIS 11-BU Pharma. (920192101) / SudocPARIS-BIUP (751062107) / SudocSudocFranceF

Determination of the van der Waals, electron donor and electron acceptor surface tension components of static Gram-positive microbial biofilms

A large number of studies have shown the influence of the physico-chemical properties of a surface on microbial adhesion phenomenon. In this study, we considered that the presence of a bacterial biofilm may be regarded as a "conditioning film" that may modify the physico-chemical characteristics of the support, and thus the adhesion capability of planktonic micro-organisms coming into contact with this substratum. In this context, we adapted a protocol for biofilm formation that allows, under our experimental conditions, contact angle measurements, the reference method to determine the energetic surface properties of a substratum. This made it possible to determine the van der Waals, electron acceptor and electron donor properties of static biofilms grown at 25 degrees C on stainless-steel slides with six Gram-positive bacteria isolated in dairy plants. A variance analysis indicated significant effects (P<0.05) of the bacterial strains and of the physiological state of the micro-organisms (planktonic or sessile) on the contact angles. To link the energetic properties of the six biofilms with direct adhesion experiments, we measured the affinity of fluorescent carboxylate-modified polystyrene beads for the different biofilm surfaces. The results correlated best with the electron-acceptor components of the biofilm surface energies, stressing the importance of Lewis acid-base interactions in adhesion mechanisms

Corrosion behaviour of AISI 304 stainless steel in presence of a biosurfactant produced by Pseudomonas fluorescens

International audienceUnder certain conditions, some microorganisms are able to synthesize surface active compounds called biosurfactants (BS), which reduce the surface tension of water. BS characteristics depend on which microorganism produce them and therefore, on the microorganism culture conditions (temperature, pH, C, N-source,...). Numerous applications are known for these biomolecules, such as cleaning, bioremediation, and their use as a detergent, or in cosmetic formulations.Recently, a large amount of literature has been edited on the influence of BS on the interactions between pathogenic bacteria and inert surfaces. It has been shown that the modifications of surface properties by the adsorption of BS can reduce microbial adhesion.Some other studies on chemical surfactants have shown that the adsorption of surface-active compounds plays a major role in corrosion; they are indeed used as an interesting corrosion inhibition tool. Therefore, it seems very interesting to study the impact of BS as environment-friendly (since biological and biodegradable) corrosion inhibitors.In the present work, an attempt was made to study the corrosion behaviour of AISI 304 stainless steel in presence of BS produced by a Gram-negative bacteria, Pseudomonas fluorescens (Pf495). Corrosion tests were achieved on several surface oxidation states. The surface morphology of the corroded specimens was investigated using SEM

Pit formation on stainless steel surfaces pre-treated with biosurfactants produced by Pseudomonas fluorescens

International audienceToday, it is widely established that the surface tension of water can be reduced by some microorganisms capable of synthesizing surface-active compounds called biosurfactants (BS). BS characteristics depend on the microorganism that produces them and therefore, on the microorganism culture conditions.Some studies on chemical surfactants have shown that the adsorption of surface-active compounds plays a major role in corrosion: indeed they are used as a good corrosion inhibition tool.The purpose of this study was first, to estimate the importance and behavior of the stainless steels passive film on the adsorption of BS, produced by the Gram negative bacteria Pseudomonas fluorescens, and secondly, to study the impact of these treatments on the pitting corrosion.In this paper, the galvanostatic polarization technique. used as accelerated method for determining the characteristic pit potentials on stainless steels, is examined. Pit growth, shape and cover formation were also observed. The surface topography of the corroded specimens was investigated using field emission scanning electron microscopy (FESEM)