245 research outputs found
Urinary reference values and first insight into the urinary proteome of captive giraffes
Urinalysis is widely recognized to be a useful tool in routine health investigations, since it can diagnose numerous pathologies. Considering the paucity of knowledge concerning giraffes, urine from 44 giraffes (Giraffa camelopardalis) (18 males and 26 females, from 3 months of age to 21 years of age) underwent routine urinalysis, 1D-electrophoresis, and protein identification using mass spectrometry, with the aim of identifying the urinary reference values and the urine proteome. The urine specific gravity (USG), urine total proteins (uTP), urine creatinine (uCr), and urine protein:creatinine ratio (UPC) reference values, reported as the median, and lower limit (LL) and upper limit (UL), were 1.030 (1006\u20131.049), 17.58 (4.54\u201335.31) mg/dL, 154.62 (39.59\u2013357.95) mg/dL, and 0.11 (0.07\u20130.16), respectively. Mass spectrometry, together with electrophoresis, revealed a pattern of common urinary proteins; albumin, lysozyme C, and ubiquitin were the most represented proteins in the giraffe urine. It has been hypothesized that these proteins could act as a defense against microbes. Moreover, in giraffes, urinalysis could be a valid tool for gauging renal function and physiological status changes
Essential (Mg, fe, zn and cu) and non-essential (cd and pb) elements in predatory insects (vespa crabro and vespa velutina): A molecular perspective
The recent introduction of the Asian yellow-legged hornet, Vespa velutina, into Europe has raised concern regarding the threat to honeybees and the competition with the European hornet, Vespa crabro. The aim of this study was to investigated essential (Mg, Fe, Zn, Cu) and non-essential (Cd and Pb) elements in these two species. Element concentrations were determined in the whole body and separately in the head, thorax and abdomen using atomic absorption spectrometry (AAS). The changes in essential element concentration and speciation during metamorphosis were also studied using size exclusion chromatography followed by AAS and proteomic analysis. In both species, the essential elements were more concentrated in the abdomen due to the presence of fat bodies. Magnesium, Fe and Zn concentrations were significantly higher in V. crabro than in V. velutina and could have been related to the higher aerobic energy demand of the former species required to sustain foraging flight. Low concentrations of Cd and Pb were indicative of low environmental exposure. The concentration and speciation of essential elements, particularly Fe, varied among the developmental stages, indicating a modification of ligand preferences during metamorphosis. Overall, the results in the present study provide a better understanding of the hornet metal metabolism and a foundation for additional studies
A machine learning approach to study demographic alterations in honeybee colonies using SDS\u2013PAGE fingerprinting
Honeybees, as social insects, live in highly organised colonies where tasks reflect the age of individuals. As is widely known, in this context, emergent properties arise from interactions between them. The accelerated maturation of nurses into foragers, stimulated by many negative fac-tors, may disrupt this complex equilibrium. This complexity needs a paradigm shift: from the study of a single stressor to the study of the effects exerted by multiple stressors on colony homeostasis. The aim of this research is, therefore, to study colony population disturbances by discriminating overaged nurses from proper aged nurses and precocious foragers from proper aged foragers using SDS-PAGE patterns of haemolymph proteins and a machine-learning algorithm. The KNN (K Near-est Neighbours) model fitted on the forager dataset showed remarkably good performances (accu-racy 0.93, sensitivity 0.88, specificity 1.00) in discriminating precocious foragers from proper aged ones. The main strength of this innovative approach lies in the possibility of it being deployed as a preventive tool. Depopulation is an elusive syndrome in bee pathology and early detection with the method described could shed more light on the phenomenon. In addition, it enables countermeas-ures to revert this vicious circle
Self-guided wakefield experiments driven by petawatt class ultra-short laser pulses
We investigate the extension of self-injecting laser wakefield experiments to
the regime that will be accessible with the next generation of petawatt class
ultra-short pulse laser systems. Using linear scalings, current experimental
trends and numerical simulations we determine the optimal laser and target
parameters, i.e. focusing geometry, plasma density and target length, that are
required to increase the electron beam energy (to > 1 GeV) without the use of
external guiding structures.Comment: 15 pages, 8 figure
Dura mater marsupialisation and outcome in a cat with a spinal subarachnoid pseudocyst: a case report
A six-month-old male domestic shorthair cat was referred with a history of acute-onset paraplegia,
over the previous two months. The neurological examination revealed a thoracolumbar lesion. After myelography
and myelo-computed tomography (myelo-CT), the diagnosis of a T13\u2013L1 subarachnoid pseudocyst potentially
related to a previous L1 vertebral body fracture or malformation was made. Surgical decompression consisted
in dorsal laminectomy followed by durotomy and marsupialisation. Immediately after surgery the cat improved
neurologically and showed progressive improvement of his neurological signs over the next few months, until he
died, from unrelated causes, approximately 18 months after surgery
A Bright Spatially-Coherent Compact X-ray Synchrotron Source
Each successive generation of x-ray machines has opened up new frontiers in
science, such as the first radiographs and the determination of the structure
of DNA. State-of-the-art x-ray sources can now produce coherent high brightness
keV x-rays and promise a new revolution in imaging complex systems on nanometre
and femtosecond scales. Despite the demand, only a few dedicated synchrotron
facilities exist worldwide, partially due the size and cost of conventional
(accelerator) technology. Here we demonstrate the use of a recently developed
compact laser-plasma accelerator to produce a well-collimated,
spatially-coherent, intrinsically ultrafast source of hard x-rays. This method
reduces the size of the synchrotron source from the tens of metres to
centimetre scale, accelerating and wiggling a high electron charge
simultaneously. This leads to a narrow-energy spread electron beam and x-ray
source that is >1000 times brighter than previously reported plasma wiggler and
thus has the potential to facilitate a myriad of uses across the whole spectrum
of light-source applications.Comment: 5 pages, 4 figure
Dimeric chlorite dismutase from the nitrogen-fixing cyanobacterium Cyanothece sp. PCC7425
It is demonstrated that cyanobacteria (both azotrophic and non-azotrophic) may 34 contain heme b oxidoreductases that can convert chlorite to chloride and molecular oxygen (incorrectly denominated chlorite “dismutase”, Cld). Beside the water-splitting manganese complex of photosystem II this metalloenzyme is the second known enzyme that catalyzes the formation of a covalent oxygen-oxygen bond. All cyanobacterial Clds have a truncated N-terminus and are dimeric (i.e. clade 2) proteins. As model protein, Cld from Cyanothece sp. PCC7425 (CCld) was recombinantly produced in E. coli and shown to efficiently degrade chlorite with an activity optimum at pH 5 (kcat 1144 ± 23.8 s-1, KM 162 ± 10.0 μM, catalytic efficiency (7.1 ± 0.6) × 106 M-1 s-1). The resting ferric high-spin axially symmetric heme enzyme has a standard reduction potential of the Fe(III)/Fe(II) couple of -126 ± 1.9 mV at pH 7. Cyanide mediates the formation of a low-spin complex with kon = (1.6 ± 0.1) × 105 M-1 s-1 and koff = 1.4 ± 2.9 s-1 (KD ~ 8.6 μM). Both, thermal and chemical unfolding follows a non-two state unfolding pathway with the first transition being related to the release of the prosthetic group. The obtained data are discussed with respect to known structure-function relationships of Clds. We ask for the physiological substrate and putative function of these O2-producing proteins in (nitrogen-fixing) cyanobacteria
Plasma adiabatic lapse rate
The plasma analog of an adiabatic lapse rate (or temperature variation with
height) in atmospheric physics is obtained. A new source of plasma temperature
gradient in a binary ion species mixture is found that is proportional to the
concentration gradient and difference in average ionization states .
Application to inertial-confinement-fusion implosions indicates a potentially
strong effect in plastic (CH) ablators that is not modeled with mainline
(single-fluid) simulations. An associated plasma thermodiffusion coefficient is
derived, and charge-state diffusion in a single-species plasma is also
predicted
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