783 research outputs found
Recommended from our members
Teleport
This collection consists of a critical preface exploring the similarities between serialized comic books, realist fiction and the author’s own writing. The principle discussion concerns continuity, the connecting tissue between ancillary works of fiction, chronology, the function of time in the narrative of related stories, and the function of characters beyond the stories they inhabit. The stories within the collection revolve around an eccentric ensemble of suburban youth whose demoralized and violent actions are heavily influenced by defining moments of their past
Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions
BACKGROUND:
Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR product, and the development of a signal by loss of fluorescence quenching as PCR degrades the probe. Though this so-called 'TaqMan' approach has proved easy to optimise in practice, the dual-labelled probes are relatively expensive.
RESULTS:
We have designed a new assay based on SYBR-Green I binding that is quick, reliable, easily optimised and compares well with the published assay. Here we demonstrate its general applicability by measuring copy number in three different genetic contexts; the quantification of a gene rearrangement (T-cell receptor excision circles (TREC) in peripheral blood mononuclear cells); the detection and quantification of GLI, MYC-C and MYC-N gene amplification in cell lines and cancer biopsies; and detection of deletions in the OPA1 gene in dominant optic atrophy.
CONCLUSION:
Our assay has important clinical applications, providing accurate diagnostic results in less time, from less biopsy material and at less cost than assays currently employed such as FISH or Southern blotting
Particle Acceleration at Relativistic Shocks
I review the current status of Fermi acceleration theory at relativistic
shocks. I first discuss the relativistic shock jump conditions, then describe
the non-relativistic Fermi mechanism and the differences introduced by
relativistic flows. I present numerical calculations of the accelerated
particle spectrum, and examine the maximum energy attainable by this process. I
briefly consider the minimum energy for Fermi acceleration, and a possible
electron pre-acceleration mechanism.Comment: 17 pages, 4 figures. To appear in "Relativistic Flows in
Astrophysics", A.W. Guthmann, M. Georganopoulos, A. Marcowith and K.
Manolokou, eds., Lecture Notes in Pysics, Springer Verla
Information Invariance and Quantum Probabilities
We consider probabilistic theories in which the most elementary system, a
two-dimensional system, contains one bit of information. The bit is assumed to
be contained in any complete set of mutually complementary measurements. The
requirement of invariance of the information under a continuous change of the
set of mutually complementary measurements uniquely singles out a measure of
information, which is quadratic in probabilities. The assumption which gives
the same scaling of the number of degrees of freedom with the dimension as in
quantum theory follows essentially from the assumption that all physical states
of a higher dimensional system are those and only those from which one can
post-select physical states of two-dimensional systems. The requirement that no
more than one bit of information (as quantified by the quadratic measure) is
contained in all possible post-selected two-dimensional systems is equivalent
to the positivity of density operator in quantum theory.Comment: 8 pages, 1 figure. This article is dedicated to Pekka Lahti on the
occasion of his 60th birthday. Found. Phys. (2009
Graviton Resonances in E+ E- -> MU+ MU- at Linear Colliders with Beamstrahlung and ISR Effects
Electromagnetic radiation emitted by the colliding beams is expected to play
an important role at the next generation of high energy e^+ e^- linear
collider(s). Focusing on the simplest process e+e- -> mu+ mu-, we show that
radiative effects like initial state radiation (ISR) and beamstrahlung can lead
to greatly-enhanced signals for resonant graviton modes of the Randall-Sundrum
model.Comment: 20 pages Latex, 7 eps figure
Equilibration processes in the Warm-Hot Intergalactic Medium
The Warm-Hot Intergalactic Medium (WHIM) is thought to contribute about 40-50
% to the baryonic budget at the present evolution stage of the universe. The
observed large scale structure is likely to be due to gravitational growth of
density fluctuations in the post-inflation era. The evolving cosmic web is
governed by non-linear gravitational growth of the initially weak density
fluctuations in the dark energy dominated cosmology. Non-linear structure
formation, accretion and merging processes, star forming and AGN activity
produce gas shocks in the WHIM. Shock waves are converting a fraction of the
gravitation power to thermal and non-thermal emission of baryonic/leptonic
matter. They provide the most likely way to power the luminous matter in the
WHIM. The plasma shocks in the WHIM are expected to be collisionless.
Collisionless shocks produce a highly non-equilibrium state with anisotropic
temperatures and a large differences in ion and electron temperatures. We
discuss the ion and electron heating by the collisionless shocks and then
review the plasma processes responsible for the Coulomb equilibration and
collisional ionisation equilibrium of oxygen ions in the WHIM. MHD-turbulence
produced by the strong collisionless shocks could provide a sizeable
non-thermal contribution to the observed Doppler parameter of the UV line
spectra of the WHIM.Comment: 13 pages, 4 figures, accepted for publication in Space Science
Reviews, special issue "Clusters of galaxies: beyond the thermal view",
Editor J.S. Kaastra, Chapter 8; work done by an international team at the
International Space Science Institute (ISSI), Bern, organised by J.S.
Kaastra, A.M. Bykov, S. Schindler & J.A.M. Bleeke
Factorizing the hard and soft spectator scattering contributions for the nucleon form factor F_1 at large Q^2
We investigate the soft spectator scattering contribution for the FF .
We focus our attention on factorization of the hard-collinear scale corresponding to transition from SCET-I to SCET-II. We compute the
leading order jet functions and find that the convolution integrals over the
soft fractions are logarithmically divergent. This divergency is the
consequence of the boost invariance and does not depend on the model of the
soft correlation function describing the soft spectator quarks. Using as
example a two-loop diagram we demonstrated that such a divergency corresponds
to the overlap of the soft and collinear regions. As a result one obtains large
rapidity logarithm which must be included in the correct factorization
formalism. We conclude that a consistent description of the factorization for
implies the end-point collinear divergencies in the hard and soft
spectator contributions, i.e. convolution integrals with respect to collinear
fractions are not well-defined. Such scenario can only be realized when the
twist-3 nucleon distribution amplitude has specific end-point behavior which
differs from one expected from the evolution of the nucleon distribution
amplitude. Such behavior leads to the violation of the collinear factorization
for the hard spectator scattering contribution. We suggest that the soft
spectator scattering and chiral symmetry breaking provide the mechanism
responsible for the violation of collinear factorization in case of form factor
.Comment: 25 pages, 6 figures, text is improved, few typos corrected, one
figure added, statement about end-point behavior of the nucleon DA is
formulated more accuratel
Magnetic fields in supernova remnants and pulsar-wind nebulae
We review the observations of supernova remnants (SNRs) and pulsar-wind
nebulae (PWNe) that give information on the strength and orientation of
magnetic fields. Radio polarimetry gives the degree of order of magnetic
fields, and the orientation of the ordered component. Many young shell
supernova remnants show evidence for synchrotron X-ray emission. The spatial
analysis of this emission suggests that magnetic fields are amplified by one to
two orders of magnitude in strong shocks. Detection of several remnants in TeV
gamma rays implies a lower limit on the magnetic-field strength (or a
measurement, if the emission process is inverse-Compton upscattering of cosmic
microwave background photons). Upper limits to GeV emission similarly provide
lower limits on magnetic-field strengths. In the historical shell remnants,
lower limits on B range from 25 to 1000 microGauss. Two remnants show
variability of synchrotron X-ray emission with a timescale of years. If this
timescale is the electron-acceleration or radiative loss timescale, magnetic
fields of order 1 mG are also implied. In pulsar-wind nebulae, equipartition
arguments and dynamical modeling can be used to infer magnetic-field strengths
anywhere from about 5 microGauss to 1 mG. Polarized fractions are considerably
higher than in SNRs, ranging to 50 or 60% in some cases; magnetic-field
geometries often suggest a toroidal structure around the pulsar, but this is
not universal. Viewing-angle effects undoubtedly play a role. MHD models of
radio emission in shell SNRs show that different orientations of upstream
magnetic field, and different assumptions about electron acceleration, predict
different radio morphology. In the remnant of SN 1006, such comparisons imply a
magnetic-field orientation connecting the bright limbs, with a non-negligible
gradient of its strength across the remnant.Comment: 20 pages, 24 figures; to be published in SpSciRev. Minor wording
change in Abstrac
Charged-Particle Multiplicities in Charged-Current Neutrino-- and Anti-Neutrino--Nucleus Interactions
The CHORUS experiment, designed to search for
oscillations, consists of a nuclear emulsion target and electronic detectors.
In this paper, results on the production of charged particles in a small sample
of charged-current neutrino-- and anti-neutrino--nucleus interactions at high
energy are presented. For each event, the emission angle and the ionization
features of the charged particles produced in the interaction are recorded,
while the standard kinematic variables are reconstructed using the electronic
detectors. The average multiplicities for charged tracks, the pseudo-rapidity
distributions, the dispersion in the multiplicity of charged particles and the
KNO scaling are studied in different kinematical regions. A study of
quasi-elastic topologies performed for the first time in nuclear emulsions is
also reported. The results are presented in a form suitable for use in the
validation of Monte Carlo generators of neutrino--nucleus interactions.Comment: 17 pages, 5 figure
Black Hole Spin via Continuum Fitting and the Role of Spin in Powering Transient Jets
The spins of ten stellar black holes have been measured using the
continuum-fitting method. These black holes are located in two distinct classes
of X-ray binary systems, one that is persistently X-ray bright and another that
is transient. Both the persistent and transient black holes remain for long
periods in a state where their spectra are dominated by a thermal accretion
disk component. The spin of a black hole of known mass and distance can be
measured by fitting this thermal continuum spectrum to the thin-disk model of
Novikov and Thorne; the key fit parameter is the radius of the inner edge of
the black hole's accretion disk. Strong observational and theoretical evidence
links the inner-disk radius to the radius of the innermost stable circular
orbit, which is trivially related to the dimensionless spin parameter a_* of
the black hole (|a_*| < 1). The ten spins that have so far been measured by
this continuum-fitting method range widely from a_* \approx 0 to a_* > 0.95.
The robustness of the method is demonstrated by the dozens or hundreds of
independent and consistent measurements of spin that have been obtained for
several black holes, and through careful consideration of many sources of
systematic error. Among the results discussed is a dichotomy between the
transient and persistent black holes; the latter have higher spins and larger
masses. Also discussed is recently discovered evidence in the transient sources
for a correlation between the power of ballistic jets and black hole spin.Comment: 30 pages. Accepted for publication in Space Science Reviews. Also to
appear in hard cover in the Space Sciences Series of ISSI "The Physics of
Accretion onto Black Holes" (Springer Publisher). Changes to Sections 5.2,
6.1 and 7.4. Section 7.4 responds to Russell et al. 2013 (MNRAS, 431, 405)
who find no evidence for a correlation between the power of ballistic jets
and black hole spi
- …