93 research outputs found
Comparison of Wavelet Transform and Fourier Transform based methods of Phasor Estimation for Numerical Relaying
Abstract-Estimatio
Re-engineering of bicistronic plasmid pGPD/IFN to construct fusion gene co-expressing Glyceraldehyde 3-phosphate dehydrogenase gene (GAPDH) of Edwardsiella tarda and Interferon-gamma (IFN-γ) gene of Labeo rohita (Hamilton) and its in vitro functional analysis
Edwardsiella septicemia disease in the cultured Indian major carps is caused by the fish pathogen Edwardsiella tarda and it is preventable by DNA vaccination. Here, we tried to develop a bicistronic DNA vaccine pGPD/IFN expressing the Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene of Edwardsiella tarda and Interferon-gamma (IFN-γ) gene of Labeo rohita. The vaccine showed high protective efficiency in our previous studies; however as a limitation of bicistronic construct the expression of gene cloned in second frame (B) is poor. To overcome this limitation we re-engineered the construct and designed a fusion gene co-expressing the GAPDH and IFN-γ genes as one frame with an aim to get the optimum expression of both the genes. For this purpose, a fusion insert comprising GAPDH and IFN-γ coding sequences was cloned in to pcDNA3.1(+) plasmid vector. The fusion genes' in vitro expression was confirmed in the striped snakehead fish cell line (SSN-1). Successful expression of the re-engineered fusion gene DNA vaccine in the cell line was achieved at 48h post-transfection, which was confirmed by amplifying the expression transcripts of GAPDH and IFN-γ genes. Thus, the study concludes that the re-engineered fusion vaccine pcGPD/IFN (pcDNA3.1(+) plasmid having fusion GPD/IFN) is functional and can be effectively utilized to vaccinate rohu (Labeo rohita) as it contains the species-specific immune gene (IFN-γ) as an adjuvant
Efficiency Theory: a Unifying Theory for Information, Computation and Intelligence
The paper serves as the first contribution towards the development of the
theory of efficiency: a unifying framework for the currently disjoint theories
of information, complexity, communication and computation. Realizing the
defining nature of the brute force approach in the fundamental concepts in all
of the above mentioned fields, the paper suggests using efficiency or
improvement over the brute force algorithm as a common unifying factor
necessary for the creation of a unified theory of information manipulation. By
defining such diverse terms as randomness, knowledge, intelligence and
computability in terms of a common denominator we are able to bring together
contributions from Shannon, Levin, Kolmogorov, Solomonoff, Chaitin, Yao and
many others under a common umbrella of the efficiency theory
Multi-layered genome defences in bacteria
This is the final version. Available on open access from Elsevier via the DOI in this recordData Availability: No data were used for the research described in the article.Bacteria have evolved a variety of defence mechanisms to protect against mobile genetic elements, including restriction-modification systems and CRISPR-Cas. In recent years, dozens of previously unknown defence systems (DSs) have been discovered. Notably, diverse DSs often coexist within the same genome, and some co-occur at frequencies significantly higher than would be expected by chance, implying potential synergistic interactions. Recent studies have provided evidence of defence mechanisms that enhance or complement one another. Here, we review the interactions between DSs at the mechanistic, regulatory, ecological and evolutionary levels.Biotechnology and Biological Sciences Research Council (BBSRC)Medical Research Council (MRC)Engineering and Physical Sciences Research Council (EPSRC
Peri-operative interventions producing better functional outcomes and enhanced recovery following total hip and knee arthroplasty: an evidence-based review
Antidiabetic effects of natural plant extracts via inhibition of carbohydrate hydrolysis enzymes with emphasis on pancreatic alpha amylase
<span style="font-size:11.0pt;line-height:115%; font-family:"Calibri","sans-serif";mso-ascii-theme-font:minor-latin;mso-fareast-font-family: "Times New Roman";mso-fareast-theme-font:minor-fareast;mso-hansi-theme-font: minor-latin;mso-bidi-font-family:"Times New Roman";mso-ansi-language:EN-US; mso-fareast-language:EN-US;mso-bidi-language:AR-SA">The diurnal variation of H<sub>2</sub>S levels inside a heavy water plant-Results from field testing of an indigenously made H<sub>2</sub>S monitor</span>
34-38<span style="font-size:11.0pt;line-height:115%;
font-family:" calibri","sans-serif";mso-ascii-theme-font:minor-latin;mso-fareast-font-family:="" "times="" new="" roman";mso-fareast-theme-font:minor-fareast;mso-hansi-theme-font:="" minor-latin;mso-bidi-font-family:"times="" roman";mso-ansi-language:en-us;="" mso-fareast-language:en-us;mso-bidi-language:ar-sa"="">Continuous measurement of H2S
concentration around a heavy water plant has been done during summer period
using indigenously developed H2S monitor. Diurnal spectrum of hourly
mean and maximum H2S levels has been studied and data have been
divided into 2 periods of the day. Hourly mean data have been analysed
statistically with log normal distribution and hourly maximum data with extreme
value distribution. The modal value of hourly maxima for summer period, which
works out to be 387 ppb, is much below the TLV of H2S for
occupational exposure.</span
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