Open Superbranes

Open branes ending on other branes, which may be referred to as the host branes, are studied in the superembedding formalism. The open brane, host brane and the target space in which they are both embedded are all taken to be supermanifolds. It is shown that the superspace constraints satisfied by the open brane are sufficient to determine the corresponding superspace constraints for the host branes, whose dynamics are determined by these constraints. As a byproduct, one also obtains information about the boundary of the open brane propagating in the host brane.Comment: 12 pages, late

Optimized exosome isolation protocol for cell culture supernatant and human plasma.

Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis of disease. However, there is currently limited information elucidating the most efficient methods for obtaining high yields of pure exosomes, a subset of extracellular vesicles, from cell culture supernatant and complex biological fluids such as plasma. To this end, we comprehensively characterize a variety of exosome isolation protocols for their efficiency, yield and purity of isolated exosomes. Repeated ultracentrifugation steps can reduce the quality of exosome preparations leading to lower exosome yield. We show that concentration of cell culture conditioned media using ultrafiltration devices results in increased vesicle isolation when compared to traditional ultracentrifugation protocols. However, our data on using conditioned media isolated from the Non-Small-Cell Lung Cancer (NSCLC) SK-MES-1 cell line demonstrates that the choice of concentrating device can greatly impact the yield of isolated exosomes. We find that centrifuge-based concentrating methods are more appropriate than pressure-driven concentrating devices and allow the rapid isolation of exosomes from both NSCLC cell culture conditioned media and complex biological fluids. In fact to date, no protocol detailing exosome isolation utilizing current commercial methods from both cells and patient samples has been described. Utilizing tunable resistive pulse sensing and protein analysis, we provide a comparative analysis of 4 exosome isolation techniques, indicating their efficacy and preparation purity. Our results demonstrate that current precipitation protocols for the isolation of exosomes from cell culture conditioned media and plasma provide the least pure preparations of exosomes, whereas size exclusion isolation is comparable to density gradient purification of exosomes. We have identified current shortcomings in common extracellular vesicle isolation methods and provide a potential standardized method that is effective, reproducible and can be utilized for various starting materials. We believe this method will have extensive application in the growing field of extracellular vesicle research

Compactification with Flux on K3 and Tori

We study compactifications of Type IIB string theory on a K3 \times T^2/Z_2 orientifold in the presence of RR and NS flux. We find the most general supersymmetry preserving, Poincare invariant, vacua in this model. All the complex structure moduli and some of the Kahler moduli are stabilised in these vacua. We obtain in an explicit fashion the restrictions imposed by supersymmetry on the flux, and the values of the fixed moduli. Some T-duals and Heterotic duals are also discussed, these are non-Calabi-Yau spaces. A superpotential is constructed describing these duals.Comment: Discussion of susy breaking vacua significantly altere

Type IIB Solutions with Interpolating Supersymmetries

We study type IIB supergravity solutions with four supersymmetries that interpolate between two types widely considered in the literature: the dual of Becker and Becker's compactifications of M-theory to 3 dimensions and the dual of Strominger's torsion compactifications of heterotic theory to 4 dimensions. We find that for all intermediate solutions the internal manifold is not Calabi-Yau, but has SU(3) holonomy in a connection with a torsion given by the 3-form flux. All 3-form and 5-form fluxes, as well as the dilaton, depend on one function appearing in the supersymmetry spinor, which satisfies a nonlinear differential equation. We check that the fields corresponding to a flat bound state of D3/D5-branes lie in our class of solutions. The relations among supergravity fields that we derive should be useful in studying new gravity duals of gauge theories, as well as possibly compactifications.Comment: 27pp, v2 REVTeX4, typographical fixes and minor clarifications, v3 added ref, modified discussion of RR axion slightl

New Supersymmetric String Compactifications

We describe a new class of supersymmetric string compactifications to 4d Minkowski space. These solutions involve type II strings propagating on (orientifolds of) non Calabi-Yau spaces in the presence of background NS and RR fluxes. The simplest examples have descriptions as cosets, generalizing the three-dimensional nilmanifold. They can also be thought of as twisted tori. We derive a formula for the (super)potential governing the light fields, which is generated by the fluxes and certain ``twists'' in the geometry. Detailed consideration of an example also gives strong evidence that in some cases, these exotic geometries are related by smooth transitions to standard Calabi-Yau or G2 compactifications of M-theory.Comment: 43 pages, harvmac bi

Future observational and modelling needs identified on the basis of the existing shelf data

NOWESP has compiled a vast quantity of existing data from the North-West European Shelf. Such a focused task is without precedence. It is now highly recommended that one, or a few national and international data centres or agencies should be chosen and properly supported by the EU, where all available observational data, including the NOWESP data, are collected, stored, regularly updated by the providers of the data, and made available to the researchers. International agreement must be reached on the quality control procedures and quality standards for data to be stored in these data bases. Proper arrangements should be made to preserve the economic value of the data for their "owners" without compromising use of the data by researchers or duplicating data collecting efforts. The continental shelf data needed are concentration fields of temperature, salinity, nutrients, suspended matter and chlorophyll, which can be called "climatological" fields. For this purpose at least one monthly survey on the whole European shelf is needed at least during five years, with a proper spatial resolution e.g. 1 degree by 1 degree, and at least in those areas where climatological data are now totally lacking. From the modelling point of view an alternative would be the availability of data from sufficiently representative fixed stations on the shelf, with weekly sampling for several years

Moduli Stabilization from Fluxes in a Simple IIB Orientifold

We study novel type IIB compactifications on the T^6/Z_2 orientifold. This geometry arises in the T-dual description of Type I theory on T^6, and one normally introduces 16 space-filling D3-branes to cancel the RR tadpoles. Here, we cancel the RR tadpoles either partially or fully by turning on three-form flux in the compact geometry. The resulting (super)potential for moduli is calculable. We demonstrate that one can find many examples of N=1 supersymmetric vacua with greatly reduced numbers of moduli in this system. A few examples with N>1 supersymmetry or complete supersymmetry breaking are also discussed.Comment: 49 pages, harvmac big; v2, corrected some typo

Flux Compactifications on Calabi-Yau Threefolds

The presence of RR and NS three-form fluxes in type IIB string compactification on a Calabi-Yau orientifold gives rise to a nontrivial superpotential W for the dilaton and complex structure moduli. This superpotential is computable in terms of the period integrals of the Calabi-Yau manifold. In this paper, we present explicit examples of both supersymmetric and nonsupersymmetric solutions to the resulting 4d N=1 supersymmetric no-scale supergravity, including some nonsupersymmetric solutions with relatively small values of W. Our examples arise on orientifolds of the hypersurfaces in $WP^{4}_{1,1,1,1,4}$ and $WP^{4}_{1,1,2,2,6}$. They serve as explicit illustrations of several of the ingredients which have played a role in the recent proposals for constructing de Sitter vacua of string theory.Comment: 30 pages, harvmac big; refs and minor comments adde