Indicators of replicative damage in equine tendon fibroblast monolayers

<p>Background: Superficial digital flexor tendon (SDFT) injuries of horses usually follow cumulative matrix microdamage; it is not known why the reparative abilities of tendon fibroblasts are overwhelmed or subverted. Relevant in vitro studies of this process require fibroblasts not already responding to stresses caused by the cell culture protocols. We investigated indicators of replicative damage in SDFT fibroblast monolayers, effects of this on their reparative ability, and measures that can be taken to reduce it.</p> <p>Results: We found significant evidence of replicative stress, initially observing consistently large numbers of binucleate (BN) cells. A more variable but prominent feature was the presence of numerous gammaH2AX (γH2AX) puncta in nuclei, this being a histone protein that is phosphorylated in response to DNA double-stranded breaks (DSBs). Enrichment for injury detection and cell cycle arrest factors (p53 (ser15) and p21) occurred most frequently in BN cells; however, their numbers did not correlate with DNA damage levels and it is likely that the two processes have different causative mechanisms. Such remarkable levels of injury and binucleation are usually associated with irradiation, or treatment with cytoskeletal-disrupting agents.</p> <p>Both DSBs and BN cells were greatest in subconfluent (replicating) monolayers. The DNA-damaged cells co-expressed the replication markers TPX2/repp86 and centromere protein F. Once damaged in the early stages of culture establishment, fibroblasts continued to express DNA breaks with each replicative cycle. However, significant levels of cell death were not measured, suggesting that DNA repair was occurring. Comet assays showed that DNA repair was delayed in proportion to levels of genotoxic stress.</p> <p>Conclusions: Researchers using tendon fibroblast monolayers should assess their “health” using γH2AX labelling. Continued use of early passage cultures expressing initially high levels of γH2AX puncta should be avoided for mechanistic studies and ex-vivo therapeutic applications, as this will not be resolved with further replicative cycling. Low density cell culture should be avoided as it enriches for both DNA damage and mitotic defects (polyploidy). As monolayers differing only slightly in baseline DNA damage levels showed markedly variable responses to a further injury, studies of effects of various stressors on tendon cells must be very carefully controlled.</p&gt

Analgesia after total hip replacement: epidural versus psoas compartment block

Background The objective of this paper is to evaluate the effectiveness of a psoas compartment block, as compared with an epidural, for postoperative analgesia following total hip replacement surgery. The research design was a double-blinded randomised control trail, in the setting of a university hospital. Methods Patients scheduled for hip arthroplasty received either a psoas compartment or epidural infusion of bupivacaine. The outcome measures that were examined were  postoperative pain, local anaesthetic and morphine consumption, and side effects. Results  There was no significant difference between the two groups regarding postoperative pain. Local anaesthetic and opiate consumption was significantly higher in the psoas compartment block group. Postoperative morphine as covariate had a significant influence on the mean postoperative pain. There was no significant difference between side effects in each group. Conclusion  Epidural analgesia was more effective than the psoas compartment block after hip replacement surgery. Although pain did not differ significantly, local anaesthetic and opiate consumption was significantly higher in the psoas compartment group

Galois theory and Lubin-Tate cochains on classifying spaces

We consider brave new cochain extensions F(BG +,R) → F(EG +,R), where R is either a Lubin-Tate spectrum E n or the related 2-periodic Morava K-theory K n , and G is a finite group. When R is an Eilenberg-Mac Lane spectrum, in some good cases such an extension is a G-Galois extension in the sense of John Rognes, but not always faithful. We prove that for E n and K n these extensions are always faithful in the K n local category. However, for a cyclic p-group C p r, the cochain extension F(BC p r +,E n ) → F(EC p r +, E n ) is not a Galois extension because it ramifies. As a consequence, it follows that the E n -theory Eilenberg-Moore spectral sequence for G and BG does not always converge to its expected target

Open Heterotic Strings

We classify potential cosmic strings according to the topological charge measurable outside the string core. We conjecture that in string theory it is this charge that governs the stability of long strings. This would imply that the SO(32) heterotic string can have endpoints, but not the E_8 x E_8 heterotic string. We give various arguments in support of this conclusion.Comment: 15 pages. v.2: typos, references correcte

Type IIB Solutions with Interpolating Supersymmetries

We study type IIB supergravity solutions with four supersymmetries that interpolate between two types widely considered in the literature: the dual of Becker and Becker's compactifications of M-theory to 3 dimensions and the dual of Strominger's torsion compactifications of heterotic theory to 4 dimensions. We find that for all intermediate solutions the internal manifold is not Calabi-Yau, but has SU(3) holonomy in a connection with a torsion given by the 3-form flux. All 3-form and 5-form fluxes, as well as the dilaton, depend on one function appearing in the supersymmetry spinor, which satisfies a nonlinear differential equation. We check that the fields corresponding to a flat bound state of D3/D5-branes lie in our class of solutions. The relations among supergravity fields that we derive should be useful in studying new gravity duals of gauge theories, as well as possibly compactifications.Comment: 27pp, v2 REVTeX4, typographical fixes and minor clarifications, v3 added ref, modified discussion of RR axion slightl

The sTB-B Hierarchy

We construct a new supersymmetric two boson (sTB-B) hierarchy and study its properties. We derive the conserved quantities and the Hamiltonian structures (proving the Jacobi identity) for the system. We show how this system gives the sKdV-B equation and its Hamiltonian structures upon appropriate reduction. We also describe the zero curvature formulation of this hierarchy both in the superspace as well as in components.Comment: 15 pages, Te

Further constraints on electron acceleration in solar noise storms

We reexamine the energetics of nonthermal electron acceleration in solar noise storms. A new result is obtained for the minimum nonthermal electron number density required to produce a Langmuir wave population of sufficient intensity to power the noise storm emission. We combine this constraint with the stochastic electron acceleration formalism developed by Subramanian & Becker (2005) to derive a rigorous estimate for the efficiency of the overall noise storm emission process, beginning with nonthermal electron acceleration and culminating in the observed radiation. We also calculate separate efficiencies for the electron acceleration -- Langmuir wave generation stage and the Langmuir wave -- noise storm production stage. In addition, we obtain a new theoretical estimate for the energy density of the Langmuir waves in noise storm continuum sources.Comment: Accepted for publication in Solar Physic