Soortgelijkheid van waren of diensten

(A) β2 neutralization reduces CRC metastatic development in the liver. (B and C) β2 neutralization reduces the migratory and adhesive potential of MC38 cells. (D) β1 neutralization does not reduce the adhesive potential of C26 cells. (DOCX 22 kb

Flow cytometry profiles of the cell surface glycan structures of the pancreatic adenocarcionoma cells.

<p><b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012524#pone-0012524-g002" target="_blank">Figure 2A</a></b>. Capan-1 (continous dot outline: ………), CP (spaced dot outline: . . . . . ), C31 (bold outline:_______), C32 (plain outline:______). <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012524#pone-0012524-g002" target="_blank">Figure 2B</a></b>. MDAPanc-28 (continous dot outline: ………), MP (spaced dot outline: . . . . . ), M34 (bold outline:_______), M33 (plain outline:______). Experiments were performed for triplicate. Representative cytometry histograms are shown. Anti-Le^x MAb binds to Galβ1,4[Fucα1,3]GlcNAc-; anti-SLe^x MAb binds to NeuAcα2-3Galβ1,4[Fucα1,3]GlcNAc-; anti-H2 MAb binds to [Fucα1,2]Galβ1,4GlcNAc-; anti-Le^y MAb binds to [Fucα1,2]Galβ1,4[Fucα1,3]GlcNAc-; SNA lectin (<i>Sambucus nigra</i> agglutinin) binds to NeuAcα2–6Galβ- structures.</p

ST3Gal III expression normalized to β-actin of the pancreatic adenocarcionoma cells.

<p>MDAPanc-28 parental cells, MP: MDAPanc-28 mock cells, M34 and M33: MDAPanc-28 cells transfected with the ST3Gal III gene. Capan-1<b>:</b> parental cells, CP: Capan-1 mock cells, C31 and C32: Capan-1 cells transfected with the ST3Gal III gene. Data represents the mean ± SD of 3 separate experiments, each in six replicates (n = 18). * Significantly different (<i>P<0.001</i>).</p

Cell migration assay.

<p>Capan-1 variant cells (Capan-1,CP and C31) <i>(</i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012524#pone-0012524-g005" target="_blank"><i>Figure 5A</i></a><i>)</i> and MDAPanc-28 variant cells (MDAPanc-28, MP and M34) <i>(</i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012524#pone-0012524-g005" target="_blank"><i>Figure 5B</i></a><i>)</i> were seeded onto 8 µm-pores-Type I-Collagen coated inserts, placed on top of wells containing DMEM-1% FBS and incubated at 37°C (6 h for Capan-1 model and 18 h for MDAPanc-28 model). Non-migrated cells were eliminated and migrated cells fixed, stained and counted. Results are expressed as migrated cells per well. Data represents the mean ± SD of the values obtained in 3 separate experiments, (n = 9). * Significantly different (<i>P<0.001</i>).</p

E-selectin induction on Primary Cultured Hepatic Sinusoidalendothelium (HSE) cells (Figure 4A).

<p>HSE cells were incubated with  =  anti-murine CD62 E (E-selectin) MAb or  =  isotype-matched control antibody. Parental Capan-1 cells were labelled with calcein and added to HSE control cells, TNF-α stimulated HSE cells, LPS stimulated HSE cells or IL-1β stimulated HSE cells. Results are expressed as the % Specific adhesion to HSE cells <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012524#pone.0012524-VidalVanaclocha2" target="_blank">[78]</a>. Data represents the mean ± SD of 3 separate experiments, each in three replicates (n = 9). * Significantly different (<i>P<0.001</i>) when comparing anti-E-selectin incubated HSEC to control cells. # Significantly different (<i>P<0.001</i>) when comparing treatments. <b>Tumour cell adhesion assay to Primary Cultured HSE cells (</b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012524#pone-0012524-g004" target="_blank"><b>Figure 4B</b></a><b>).</b> Capan-1 variant cells were labelled with Calcein and added to IL-1β stimulated HSE cells or (<b>−</b>) not stimulated HSE control cells. >  =  anti-murine CD62 E (E-selectin) MAb was added to IL-1β HSE cells or <b>-</b> HSE cells before tumour cell addition. * Significantly different (<i>P<0.001</i>) when comparing Capan-1, CP, and C31 cells. # Significantly different (<i>P<0.001</i>) when comparing each clone (Capan-1, CP and C31) adhesion for the different HSE cell treatments.</p

Binding assay to rh-E-selectin.

<p>Capan-1 variant cells (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012524#pone-0012524-g003" target="_blank"><b>Figure 3A</b></a>) and MDAPanc-28 variant cells (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0012524#pone-0012524-g003" target="_blank"><b>Figure 3B</b></a>), previously incubated with PBS-1% BSA (light bars) or anti-SLe^x MAb (dark bars), were added to 96-well microplates coated with rh E-selectin or PBS-1% BSA (negative control). Adherent cells were estimated with a MTT-based colorimetric assay. Results are expressed as the Specific binding to E-selectin (O.D. 570 nm of cells bounded to E-selectin – O.D. 570 nm of cells bonded to PBS-1% BSA) <i>versus</i> cells previously incubated or not with anti-SLe^x MAb. Data represents the mean ± SD of 3 separate experiments, each in five replicates (n = 15). * Significantly different (<i>P<0.001</i>).</p

Kaplan-Meier plots of estimated survival after injection of MP (MDAPanc-28 mock cells) and M34 (MDAPanc-28 ST3Gal III transfected cells).

<p>Cells (7×10⁶) were intrasplenically injected in nude mice on day 1 of the experiment. Mice were daily examined and sacrificed when they looked sick. The differences between groups were assessed by the long-rank test (<i>P</i> = 0.019; n = 7–8/group).</p

Study of the carbohydrate/protein composition in different strains and morphologies.

<p>(A) Carbohydrate/protein ratio in the crude extracts of the different strains of <i>Candida albicans</i>: Blastoconidia (□) and germ tubes (▪). The results shown correspond to the mean ± SD of three independent experiments. Statistically significant differences between different morphologies are indicated by two asterisks (**) (p<0.05). (B). Regression line for the B16 melanoma (B16M) cell adhesion to hepatic sinusoidal endothelial (HSE) cells induced by the strains and morphologies of <i>C. albicans</i> that lead a significant effect <i>versus</i> their carbohydrate/protein ratio.</p

Effect of the mannose receptor (MR) inhibition.

<p>Effect of the anti-mannose receptor antibodies (anti-MR) on the inhibition of the synthesis of IL-18 (A) and tumor cell adhesion to hepatic sinusoidal endothelial (HSE) cells (B) induced by <i>Candida albicans</i> UPV1413 and its mannoprotein-enriched fraction (MPF): basal medium (□), 30 µg/ml anti-MR (▪). The results shown correspond to the mean ± SD of three independent experiments. Statistically significant differences with respect to the controls or to the cells without antibodies are indicated by one (*) and two asterisks (**) (p<0.05), respectively.</p

Results obtained from the killing treatments.

<p>(A–D) Effect of heat and metaperiodate treatments on <i>Candida albicans</i> cell surface studied by incubation with Con A-FITC followed by flow cytometry: cell complexity (side scatter, SSC), cell size (Forward Scatter, FSC), and fluorescence (FL1). R1: live cells. R2: cells killed with heat. R3: cells killed with a 20 mM metaperiodate. R4: cells killed with 50 mM metaperiodate. (E) Effect of heat- and sodium metaperiodate (50 mM)-treated <i>C. albicans</i> blastoconidia on the increase of B16 melanoma (B16M) cell adhesion to HSE. The results shown correspond to the mean ± SD of three independent experiments. Statistically significant differences with respect to the control or between cell conditions are indicated by one and two asterisks (**) (p<0.05), respectively.</p