3,034 research outputs found
Subunit rearrangement of the cyclin-dependent kinases is associated with cellular transformation
In normal human diploid fibroblasts, cyclins of the A, B, and D classes each associate with cyclin-dependent kinases (CDKs), proliferating cell nuclear antigen (PCNA), and p21, thereby forming multiple independent quaternary complexes. Upon transformation of diploid fibroblasts with the DNA tumor virus SV40, or its transforming tumor antigen (T), the cyclin D/p21/CDK/PCNA complexes are disrupted. In transformed cells, CDK4 totally dissociates from cyclin D, PCNA, and p21 and, instead, associates exclusively with a polypeptide of 16 kD (p16). Quaternary complexes containing cyclins A or B1 and p21/CDK/PCNA also undergo subunit rearrangement in transformed cells. Both PCNA and p21 are no longer associated with CDC2-cyclin B1 binary complexes. Cyclin A complexes no longer contain p21, and a new 19-kD polypeptide (p19) is found in association with cyclin A. The pattern of subunit rearrangement of cyclin-CDK complexes in SV40-transformed cells is also shared in those containing adeno- or papilloma viral oncoproteins. Rearrangement also occurs in p53-deficient cells derived from Li-Fraumeni patients that carry no known DNA tumor virus. These findings suggest a mechanism by which oncogenic proteins alter the cell cycle of transformed cells
Photovoltaics, The Solar Electric Solution
Direct conversion of solar energy to electricity by photovoltaic devices (solar cells) offers what may be the most promising of nearterm solutions to our energy problems. The federal government has been pushing the commercialization of photovoltaics by funding research on new materials and manufacturing processes.
Earliest commercialization is expected in residential systems where the power grid provides the backup. The Florida Solar Energy Center has been operating a 5 kw experimental residential facility for several months with encouraging results. Although the present payback rate is not attractive, the system is reliable and has produced 500 to 600 kilowatt- hours of energy in the poorer winter months at Cape Canaveral
Site dilution of quantum spins in the honeycomb lattice
We discuss the effect of site dilution on both the magnetization and the
density of states of quantum spins in the honeycomb lattice, described by the
antiferromagnetic Heisenberg spin-S model. For this purpose a real-space
Bogoliubov-Valatin transformation is used. In this work we show that for the
S>1/2 the system can be analyzed in terms of linear spin wave theory. For spin
S=1/2, however, the linear spin wave approximation breaks down. In this case,
we have studied the effect of dilution on the staggered magnetization using the
Stochastic Series Expansion Monte Carlo method. Two main results are to be
stressed from the Monte Carlo method: (i) a better value for the staggered
magnetization of the undiluted system, m=0.2677(6); (ii) a finite value of the
staggered magnetization of the percolating cluster at the classical percolation
threshold, showing that there is no quantum critical transition driven by
dilution in the Heisenberg model. In the solution of the problem using linear
the spin wave method we pay special attention to the presence of zero energy
modes. Using a combination of linear spin wave analysis and the recursion
method we were able to obtain the thermodynamic limit behavior of the density
of states for both the square and the honeycomb lattices. We have used both the
staggered magnetization and the density of states to analyze neutron scattering
experiments and Neel temperature measurements on quasi-two- -dimensional
honeycomb systems. Our results are in quantitative agreement with experimental
results on Mn_pZn_{1-p}PS_3 and on the Ba(Ni_pMg_{1-p})_2V_2O_8.Comment: 21 pages (REVTEX), 16 figure
p34cdc2-mediated phosphorylation at T124 inhibits nuclear import of SV-40 T antigen proteins
The nuclear import of transcription regulatory proteins appears to be used by the cell to trigger transitions in cell cycle, morphogenesis, and transformation. We have previously observed that the rate at which SV-40 T antigen fusion proteins containing a functional nuclear localization sequence (NLS; residues 126-132) are imported into the nucleus is enhanced in the presence of the casein kinase II (CK-II) site S111/112. In this study purified p34cdc2 kinase was used to phosphorylate T antigen proteins specifically at T124 and kinetic measurements at the single-cell level performed to assess its effect on nuclear protein import. T124 phosphorylation, which could be functionally simulated by a T-to-D124 substitution, was found to reduce the maximal extent of nuclear accumulation whilst negligibly affecting the import rate. The inhibition of nuclear import depended on the stoichiometry of phosphorylation. T124 and S111/112 could be phosphorylated independently of one another. Two alterative mechanisms were considered to explain the inhibition of nuclear import by T124 phosphorylation: inactivation of the NLS and cytoplasmic retention, respectively. Furthermore, we speculate that in vivo T124 phosphorylation may regulate the small but functionally significant amount of cytoplasmic SV-40 T antigen. A sequence comparison showed that many transcription regulatory proteins contain domains comprising potential CK-II-sites, cdc2-sites, and NLS. This raises the possibility that the three elements represent a functional unit regulating nuclear protein import
Team 6: Joint Capability Metamodel-Test-Metamodel Integration with Data Farming
from Scythe : Proceedings and Bulletin of the International Data Farming Community, Issue 2 Workshop 14US adversaries are continuously seeking new ways to
threaten US interests at home and abroad. In order to
counter these threats, now more than ever,
commanders must seek to leverage existing and
emerging joint capabilities effectively in a variety of
unique contexts. Achieving mission effectiveness in
today's joint operational environment demands robust
synergy among a wide array of mission-critical Service
systems and capabilities
A reliable Pade analytical continuation method based on a high accuracy symbolic computation algorithm
We critique a Pade analytic continuation method whereby a rational polynomial
function is fit to a set of input points by means of a single matrix inversion.
This procedure is accomplished to an extremely high accuracy using a novel
symbolic computation algorithm. As an example of this method in action we apply
it to the problem of determining the spectral function of a one-particle
thermal Green's function known only at a finite number of Matsubara frequencies
with two example self energies drawn from the T-matrix theory of the Hubbard
model. We present a systematic analysis of the effects of error in the input
points on the analytic continuation, and this leads us to propose a procedure
to test quantitatively the reliability of the resulting continuation, thus
eliminating the black magic label frequently attached to this procedure.Comment: 11 pages, 8 eps figs, revtex format; revised version includes
reference to anonymous ftp site containing example codes (MapleVr5.1
worksheets) displaying the implementation of the algorithm, including the
padematinv.m library packag
Peierls to superfluid crossover in the one-dimensional, quarter-filled Holstein model
We use continuous-time quantum Monte Carlo simulations to study retardation
effects in the metallic, quarter-filled Holstein model in one dimension. Based
on results which include the one- and two-particle spectral functions as well
as the optical conductivity, we conclude that with increasing phonon frequency
the ground state evolves from one with dominant diagonal order---2k_F charge
correlations---to one with dominant off-diagonal fluctuations, namely s-wave
pairing correlations. In the parameter range of this crossover, our numerical
results support the existence of a spin gap for all phonon frequencies. The
crossover can hence be interpreted in terms of preformed pairs corresponding to
bipolarons, which are essentially localised in the Peierls phase, and
"condense" with increasing phonon frequency to generate dominant pairing
correlations.Comment: 11 pages, 5 figure
Chiral magnetization textures stabilized by the Dzyaloshinskii-Moriya interaction during spin-orbit torque switching
We study the effect of the Dzyaloshinskii-Moriya interaction (DMI) on
current-induced magnetic switching of a perpendicularly magnetized
heavy-metal/ferromagnet/oxide trilayer both experimentally and through
micromagnetic simulations. We report the generation of stable helical
magnetization stripes for a sufficiently large DMI strength in the switching
region, giving rise to intermediate states in the magnetization confirming the
essential role of the DMI on switching processes. We compare the simulation and
experimental results to a macrospin model, showing the need for a micromagnetic
approach. The influence of the temperature on the switching is also discussed.Comment: Includes corrected acknowledgements and clarification of simulation
parameter
Investigation of the Dzyaloshinskii-Moriya interaction and room temperature skyrmions in W/CoFeB/MgO thin films and microwires
Recent studies have shown that material structures, which lack structural
inversion symmetry and have high spin-orbit coupling can exhibit chiral
magnetic textures and skyrmions which could be a key component for next
generation storage devices. The Dzyaloshinskii-Moriya Interaction (DMI) that
stabilizes skyrmions is an anti-symmetric exchange interaction favoring
non-collinear orientation of neighboring spins. It has been shown that material
systems with high DMI can lead to very efficient domain wall and skyrmion
motion by spin-orbit torques. To engineer such devices, it is important to
quantify the DMI for a given material system. Here we extract the DMI at the
Heavy Metal (HM) /Ferromagnet (FM) interface using two complementary
measurement schemes namely asymmetric domain wall motion and the magnetic
stripe annihilation. By using the two different measurement schemes, we find
for W(5 nm)/Co20Fe60B20(0.6 nm)/MgO(2 nm) the DMI to be 0.68 +/- 0.05 mJ/m2 and
0.73 +/- 0.5 mJ/m2, respectively. Furthermore, we show that this DMI stabilizes
skyrmions at room temperature and that there is a strong dependence of the DMI
on the relative composition of the CoFeB alloy. Finally we optimize the layers
and the interfaces using different growth conditions and demonstrate that a
higher deposition rate leads to a more uniform film with reduced pinning and
skyrmions that can be manipulated by Spin-Orbit Torques
Navigating noisy waters: A review of field studies examining anthropogenic noise effects on wild fish
Anthropogenic noise is globally increasing in aquatic ecosystems, and there is concern that it may have adverse consequences in many fish species, yet the effects of noise in field settings are not well understood. Concern over the applicability of laboratory-conducted bioacoustic experiments has led to a call for, and a recent increase in, fieldbased studies, but the results have been mixed, perhaps due to the wide variety of techniques used and species studied. Previous reviews have explored the behavioral, physiological, and/or anatomical costs of fish exposed to anthropogenic noise, but few, if any, have focused on the field techniques and sound sources themselves. This review, therefore, aims to summarize, quantify, and interpret field-based literature, highlight novel approaches, and provide recommendations for future research into the effects of noise on fish. VC 2023 Acoustical Society of America
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