Mortality of fish subjected to explosive shock as applied to oil well severance on Georges Bank

A very extensive bibliography of papers on underwater explosions and their effects on marine life has been collected and summarized. When exposed to blast effects, vertebrates with swim bladders or lungs that contain gas are at least an order of magnitude more sensitive than other life. Regression analysis of several different experiments on explosive damage to fish has been combined with reports of fish concentrations and explosives used in oil well severance in order to estimate the probable extent of damage to fish populations from a limited number of severance explosions. Damage per explosion should not be significant and is probably considerably less than that caused by a one hour tow of a bottom trawl net.Prepared for the Technology Assessment and Research Program of the Minerals Management Service, Department of the Interior, under Contract 14-08-0001-18920

ERβ Binds N-CoR in the Presence of Estrogens via an LXXLL-like Motif in the N-CoR C-terminus

Nuclear receptors (NRs) usually bind the corepressors N-CoR and SMRT in the absence of ligand or in the presence of antagonists. Agonist binding leads to corepressor release and recruitment of coactivators. Here, we report that estrogen receptor β (ERβ) binds N-CoR and SMRT in the presence of agonists, but not antagonists, in vitro and in vivo. This ligand preference differs from that of ERα interactions with corepressors, which are inhibited by estradiol, and resembles that of ERβ interactions with coactivators. ERβ /N-CoR interactions involve ERβ AF-2, which also mediates coactivator recognition. Moreover, ERβ recognizes a sequence (PLTIRML) in the N-CoR C-terminus that resembles coactivator LXXLL motifs. Inhibition of histone deacetylase activity specifically potentiates ERβ LBD activity, suggesting that corepressors restrict the activity of AF-2. We conclude that the ER isoforms show completely distinct modes of interaction with a physiologically important corepressor and discuss our results in terms of ER isoform specificity in vivo

Random Tilings: Concepts and Examples

We introduce a concept for random tilings which, comprising the conventional one, is also applicable to tiling ensembles without height representation. In particular, we focus on the random tiling entropy as a function of the tile densities. In this context, and under rather mild assumptions, we prove a generalization of the first random tiling hypothesis which connects the maximum of the entropy with the symmetry of the ensemble. Explicit examples are obtained through the re-interpretation of several exactly solvable models. This also leads to a counterexample to the analogue of the second random tiling hypothesis about the form of the entropy function near its maximum.Comment: 32 pages, 42 eps-figures, Latex2e updated version, minor grammatical change

Evolutionary Novelty in a Butterfly Wing Pattern through Enhancer Shuffling.

An important goal in evolutionary biology is to understand the genetic changes underlying novel morphological structures. We investigated the origins of a complex wing pattern found among Amazonian Heliconius butterflies. Genome sequence data from 142 individuals across 17 species identified narrow regions associated with two distinct red colour pattern elements, dennis and ray. We hypothesise that these modules in non-coding sequence represent distinct cis-regulatory loci that control expression of the transcription factor optix, which in turn controls red pattern variation across Heliconius. Phylogenetic analysis of the two elements demonstrated that they have distinct evolutionary histories and that novel adaptive morphological variation was created by shuffling these cis-regulatory modules through recombination between divergent lineages. In addition, recombination of modules into different combinations within species further contributes to diversity. Analysis of the timing of diversification in these two regions supports the hypothesis of introgression moving regulatory modules between species, rather than shared ancestral variation. The dennis phenotype introgressed into Heliconius melpomene at about the same time that ray originated in this group, while ray introgressed back into H. elevatus much more recently. We show that shuffling of existing enhancer elements both within and between species provides a mechanism for rapid diversification and generation of novel morphological combinations during adaptive radiation.This work was funded by BBSRC grant H01439X/1, ERC grant MimEvol and ANR grant HybEvol to MJ.This is the final version of the article. It was first available from PLOS via http://dx.doi.org/10.1371/journal.pbio.100235

Density of states, Potts zeros, and Fisher zeros of the Q-state Potts model for continuous Q

The Q-state Potts model can be extended to noninteger and even complex Q in the FK representation. In the FK representation the partition function,Z(Q,a), is a polynomial in Q and v=a-1(a=e^-T) and the coefficients of this polynomial,Phi(b,c), are the number of graphs on the lattice consisting of b bonds and c connected clusters. We introduce the random-cluster transfer matrix to compute Phi exactly on finite square lattices. Given the FK representation of the partition function we begin by studying the critical Potts model Z_{CP}=Z(Q,a_c), where a_c=1+sqrt{Q}. We find a set of zeros in the complex w=sqrt{Q} plane that map to the Beraha numbers for real positive Q. We also identify tilde{Q}_c(L), the value of Q for a lattice of width L above which the locus of zeros in the complex p=v/sqrt{Q} plane lies on the unit circle. We find that 1/tilde{Q}_c->0 as 1/L->0. We then study zeros of the AF Potts model in the complex Q plane and determine Q_c(a), the largest value of Q for a fixed value of a below which there is AF order. We find excellent agreement with Q_c=(1-a)(a+3). We also investigate the locus of zeros of the FM Potts model in the complex Q plane and confirm that Q_c=(a-1)^2. We show that the edge singularity in the complex Q plane approaches Q_c as Q_c(L)~Q_c+AL^-y_q, and determine the scaling exponent y_q. Finally, by finite size scaling of the Fisher zeros near the AF critical point we determine the thermal exponent y_t as a function of Q in the range 2<Q<3. We find that y_t is a smooth function of Q and is well fit by y_t=(1+Au+Bu^2)/(C+Du) where u=u(Q). For Q=3 we find y_t~0.6; however if we include lattices up to L=12 we find y_t~0.50.Comment: to appear in Physical Review

The beginning of time? Evidence for catastrophic drought in Baringo in the early nineteenth century

New developments in the collection of palaeo-data over the past two decades have transformed our understanding of climate and environmental history in eastern Africa. This article utilises instrumental and proxy evidence of historical lake-level fluctuations from Baringo and Bogoria, along with other Rift Valley lakes, to document the timing and magnitude of hydroclimate variability at decadal to century time scales since 1750. These data allow us to construct a record of past climate variation not only for the Baringo basin proper, but also across a sizable portion of central and northern Kenya. This record is then set alongside historical evidence, from oral histories gathered amongst the peoples of northern Kenya and the Rift Valley and from contemporary observations recorded by travellers through the region, to offer a reinterpretation of human activity and its relationship to environmental history in the nineteenth century. The results reveal strong evidence of a catastrophic drought in the early nineteenth century, the effects of which radically alters our historical understanding of the character of settlement, mobility and identity within the Baringo–Bogoria basin

Effects of the nicotinic agonist varenicline, nicotinic antagonist r-bPiDI, and DAT inhibitor R-modafinil on co-use of ethanol and nicotine in female P rats.

Rationale: Co-users of alcohol and nicotine are the largest group of polysubstance users worldwide. Commonalities in mechanisms of action for ethanol (EtOH) and nicotine proposes the possibility of developing a single pharmacotherapeutic to treat co-use. Objectives: Toward developing a preclinical model of co-use, female alcohol-preferring (P) rats were trained for voluntary EtOH drinking and i.v. nicotine self-administration in three phases: (1) EtOH alone (0 vs. 15%, 2-bottle choice); (2) nicotine alone (0.03 mg/kg/infusion, active vs. inactive lever); and (3) concurrent access to both EtOH and nicotine. Using this model, we examined the effects of (1) varenicline, a nicotinic acetylcholine receptor (nAChR) partial agonist with high affinity for the α4β2 subtype; (2) r-bPiDI, a subtype-selective antagonist at α6β2* nAChRs; and (3) (R)-modafinil, an atypical inhibitor of the dopamine transporter (DAT). Results: In Phases 1 and 2, pharmacologically relevant intake of EtOH and nicotine was achieved. In the concurrent access phase (Phase 3), EtOH consumption decreased while nicotine intake increased relative to Phases 1 and 2. For drug pretreatments, in the EtOH access phase (Phase 1), (R)-modafinil (100 mg/kg) decreased EtOH consumption, with no effect on water consumption. In the concurrent access phase, varenicline (3 mg/kg), r-bPiDI (20 mg/kg), and (R)-modafinil (100 mg/kg) decreased nicotine self-administration, but did not alter EtOH consumption, water consumption, or inactive lever pressing. Conclusions: These results indicate that therapeutics which may be useful for smoking cessation via selective inhibition of α4β2 or α6β2* nAChRs, or DAT inhibition, may not be sufficient to treat EtOH and nicotine co-use