Antigen presentation to B cells

B cells are capable of mounting responses to a bewildering range of potentially pathogenic antigens through the production of high-affinity antibodies and the establishment of immunological memory. Thus, regulated B-cell activation is critical for protection against a variety of bacterial and viral infections, as well as cancers. Here, we discuss a number of recent imaging studies that have provided new insights into the variety of mechanisms by which B-cell activation is initiated in the lymph node in vivo

A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes

Cells such as fibroblasts and endothelial cells migrate through the coordinated responses of discrete integrin-containing focal adhesions and complexes. In contrast, little is known about the organization of integrins on the highly motile T lymphocyte. We have investigated the distribution, activity, and cytoskeletal linkage of the integrin lymphocyte function associated antigen-1 (LFA-1) on human T lymphocytes migrating on endothelial cells and on ligand intercellular adhesion molecule-1 (ICAM-1). The pattern of total LFA-1 varies from low expression in the lamellipodia to high expression in the uropod. However, high affinity, clustered LFA-1 is restricted to a mid-cell zone that remains stable over time and over a range of ICAM-1 densities. Talin is essential for the stability and formation of the LFA-1 zone. Disruption of the talin–integrin link leads to loss of zone integrity and a substantial decrease in speed of migration on ICAM-1. This adhesive structure, which differs from the previously described integrin-containing attachments displayed by many other cell types, we have termed the “focal zone.

Daughter B cells are not created equal: Asymmetric segregation of antigen during B cell division

Comment on: Thaunat O, et al. Science 2012; 335:475-9

Phospholipase C-γ2 and Vav cooperate within signaling microclusters to propagate B cell spreading in response to membrane-bound antigen

B cell receptor (BCR) recognition of membrane-bound antigen initiates a spreading and contraction response, the extent of which is controlled through the formation of signaling-active BCR-antigen microclusters and ultimately affects the outcome of B cell activation. We followed a genetic approach to define the molecular requirements of BCR-induced spreading and microcluster formation. We identify a key role for phospholipase C-γ2 (PLCγ2), Vav, B cell linker, and Bruton's tyrosine kinase in the formation of highly coordinated “microsignalosomes,” the efficient assembly of which is absolutely dependent on Lyn and Syk. Using total internal reflection fluorescence microscopy, we examine at high resolution the recruitment of PLCγ2 and Vav to microsignalosomes, establishing a novel synergistic relationship between the two. Thus, we demonstrate the importance of cooperation between components of the microsignalosome in the amplification of signaling and propagation of B cell spreading, which is critical for appropriate B cell activation

Neutrophils—the unexpected helpers of B‐cell activation

A specific subpopulation of neutrophils, termed N(BH), has been shown recently to provide help for the differentiation and function of B cells and plasma cells. These novel findings are put in the context of our current understanding of B-cell help

Dynamic cortical actin remodeling by ERM proteins controls BCR microcluster organization and integrity

By dynamically remodeling the cortical actin network, ezrin and moesin control BCR microcluster formation, organization, and integrity

The S-PLUS Fornax Project (S+FP): a first 12-band glimpse of the Fornax galaxy cluster

The Fornax galaxy cluster is the richest nearby (D ∼ 20 Mpc) galaxy association in the southern sky. As such, it provides a wealth of oportunities to elucidate on the processes where environment holds a key role in transforming galaxies. Although it has been the focus of many studies, Fornax has never been explored with contiguous homogeneous wide-field imaging in 12 photometric narrow- and broad-bands like those provided by the Southern Photometric Local Universe Survey (S-PLUS). In this paper we present the S-PLUS Fornax Project (S+FP) that aims to comprehensively analyse the galaxy content of the Fornax cluster using S-PLUS. Our data set consists of 106 S-PLUS wide-field frames (FoV∼1.4 ×1.4 deg2) observed in five SDSS-like ugriz broad-bands and seven narrow-bands covering specific spectroscopic features like [OII], CaII H+K, Hδ, G-band, Mg b triplet, Hα, and the CaII triplet. Based on S-PLUS specific automated photometry, aimed at correctly detecting Fornax galaxies and globular clusters in S-PLUS images, our dataset provides the community with catalogues containing homogeneous 12-band photometry for ∼3 × 106 resolved and unresolved objects within a region extending over ∼208 deg2 (∼5 Rvir in RA) around Fornax’ central galaxy, NGC 1399. We further explore the eagle and IllustrisTNG cosmological simulations to identify 45 Fornax-like clusters and generate mock images on all 12 S-PLUS bands of these structures down to galaxies with M⋆ ≥ 108 M⊙. The S+FP dataset we put forward in this first paper of a series will enable a variety of studies some of which are briefly presented.Fil: Smith Castelli, Analia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Cortesi, A. Universidade Federal do Rio de Janeiro; BrasilFil: Haack, Rodrigo Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Lopes, A. R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Thainá Batista, J.. Universidade Federal de Santa Catarina; BrasilFil: Cid Fernandes, R.. Universidade Federal de Santa Catarina; BrasilFil: Lomelí Núñez, L.. Universidade Federal do Rio de Janeiro; BrasilFil: Ribeiro, U.. Centro Brasileiro de Pesquisas Físicas; BrasilFil: de Bom, C. R.. Centro Brasileiro de Pesquisas Físicas; BrasilFil: Cernic, V.. Universidade Do Sao Paulo. Instituto Astronomia, Geofísica E Ciencias Atmosfericas. Departamento de Astronomia; BrasilFil: Sodré, Laerte. Universidade Do Sao Paulo. Instituto Astronomia, Geofísica E Ciencias Atmosfericas. Departamento de Astronomia; BrasilFil: Zenocratti, Lucas Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: de Rossi, Maria Emilia. Universidad de Buenos Aires; ArgentinaFil: Calderón, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Herpich, F.. Cambridge Survey Astronomical Unit; Reino UnidoFil: Telles, E.. Ministério de Ciencia, Tecnologia e Innovacao. Observatorio Nacional; BrasilFil: Saha, K.. Inter University Centre For Astronomy And Astrophysics; IndiaFil: Lopes, P. A. A.. Universidade Federal do Rio de Janeiro; BrasilFil: Lopes Silva, V. H.. Universidade Federal do Rio de Janeiro; BrasilFil: Gonçalves, T. S.. Universidade Federal do Rio de Janeiro; BrasilFil: Bambrila, D.. Universidade Federal do Rio de Janeiro; BrasilFil: Cardoso, N. M.. Universidade de Sao Paulo; BrasilFil: Buzzo, M. L.. Swinburne University; AustraliaFil: Astudillo Sotomayor, P.. Universidad de Concepción; ChileFil: Demarco, R.. Universidad Andrés Bello; ChileFil: Leigh, N.. Universidad de Concepción; ChileFil: Sarzi, M.. Armagh Observatory And Planetarium, College Hill; Reino UnidoFil: Menéndez Delmestre, K.. Universidade Federal do Rio de Janeiro; BrasilFil: Faifer, Favio Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Gutierrez Soto, Luis Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; Argentin