Traumatic neuroma of the mandible: a case report with spontaneous remission

Traumatic neuroma is a well-known disorder involving peripheral nerves, which occurs following trauma or sur - gery. The lesion develops most commonly in the soft tissues of the mental foramen area, lower lip and tongue. Intra- osseous lesions arising in jawbones are very uncommon. In this paper, we report a new case of an intra-osseous traumatic neuroma, discovered incidentally on a panoramic radiograph obtained for orthodontic documentation. In addition, the case herein described developed spontaneous remission, a situation not previously reported in the literature. Finally, we discuss relevant demographic, clinical, microscopic, immunohistochemical and treatment aspects of traumatic neuromas

ASSOCIAÇÃO DO TESTE DE EXPOSIÇÃO DE CURTA DURAÇÃO (STE) COM O TESTE DE PERMEABILIDADE E OPACIDADE DA CÓRNEA BOVINA (BCOP) PARA A AVALIAÇÃO DO POTENCIAL DE IRRITAÇÃO OCULAR DE PRAGUICIDAS

Introdução e objetivos: Os praguicidas, no Brasil, são submetidos a teste de toxicidade ocular com o uso de animais, a exemplo o Teste de Draize. Considerações do bem-estar animal e proibição de ensaios toxicológicos em animais para cosméticos, fomentaram a redução e substituição de experimentos em animais por métodos alternativos. Entretanto, somente um ensaio alternativo não pode substituir completamente o Teste de Draize. Neste contexto, o objetivo do trabalho foi avaliar o potencial de irritação ocular de 12 praguicidas, correspondente a 6 herbicidas, 3 inseticidas, 2 fungicidas e 1 acaricida, através da associação do Teste de Exposição de curta duração (STE) com o teste de permeabilidade e opacidade da córnea bovina (BCOP). Metodologia: O STE foi realizado de acordo com o Draft OECD de 2014¹. O BCOP foi realizado conforme o protocolo OECD 437 de 2013², seguido de análises histopatológicas com coloração hematoxilina e eosina. Os praguicidas classificados como irritante no STE foram submetidos ao BCOP. Resultados e discussões: Dos 12 praguicidas avaliados no STE, 4 foram classificados como Categoria 1 (irritante), 5 como incapaz de classificar e 3 como sem categoria (não irritante). No BCOP, 1 praguicida foi classificado como Categoria 1 (irritante) e 3 como nenhuma previsão pode ser feita (sem classificação neste método)³. Porém, nas análises histopatológicas foram observados que todos os praguicidas causaram danos a córnea. Conclusões: Portanto, o herbicida ariloxialcanóico, inseticida organofosforado, fungicida triazólico e inseticida biológico foram classificados como irritante no STE. E no BCOP somente o inseticida organofosforado foi classificado como irritante. A associação do STE com BCOP apresentou limitações na avaliação de toxicidade ocular de praguicidas

Combined effect of bumetanide, bromide, and GABAergic agonists: An alternative treatment for intractable seizures

Univ Fed Sao Joao del Rei, Lab Neurociencia Expt & Computac, Programa Inst Bioengn, Sao Joao Del Rei, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Expt Neurol, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Fisiol, São Paulo, BrazilPontificia Univ Catolica Rio Grande do Su, Inst Cerebro, Porto Alegre, RS, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Expt Neurol, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Fisiol, São Paulo, BrazilWeb of Scienc

Environmental Shaping of Sponge Associated Archaeal Communities

Archaea are ubiquitous symbionts of marine sponges but their ecological roles and the influence of environmental factors on these associations are still poorly understood.We compared the diversity and composition of archaea associated with seawater and with the sponges Hymeniacidon heliophila, Paraleucilla magna and Petromica citrina in two distinct environments: Guanabara Bay, a highly impacted estuary in Rio de Janeiro, Brazil, and the nearby Cagarras Archipelago. For this we used metagenomic analyses of 16S rRNA and ammonia monooxygenase (amoA) gene libraries. Hymeniacidon heliophila was more abundant inside the bay, while P. magna was more abundant outside and P. citrina was only recorded at the Cagarras Archipelago. Principal Component Analysis plots (PCA) generated using pairwise unweighted UniFrac distances showed that the archaeal community structure of inner bay seawater and sponges was different from that of coastal Cagarras Archipelago. Rarefaction analyses showed that inner bay archaeaoplankton were more diverse than those from the Cagarras Archipelago. Only members of Crenarchaeota were found in sponge libraries, while in seawater both Crenarchaeota and Euryarchaeota were observed. Although most amoA archaeal genes detected in this study seem to be novel, some clones were affiliated to known ammonia oxidizers such as Nitrosopumilus maritimus and Cenarchaeum symbiosum.The composition and diversity of archaeal communities associated with pollution-tolerant sponge species can change in a range of few kilometers, probably influenced by eutrophication. The presence of archaeal amoA genes in Porifera suggests that Archaea are involved in the nitrogen cycle within the sponge holobiont, possibly increasing its resistance to anthropogenic impacts. The higher diversity of Crenarchaeota in the polluted area suggests that some marine sponges are able to change the composition of their associated archaeal communities, thereby improving their fitness in impacted environments

Metagenomic analysis of a tropical composting operation at the São Paulo Zoo Park reveals diversity of biomass degradation functions and organisms.

Composting operations are a rich source for prospection of biomass degradation enzymes. We have analyzed the microbiomes of two composting samples collected in a facility inside the Sao Paulo Zoo Park, in Brazil. All organic waste produced in the park is processed in this facility, at a rate of four tons/day. Total DNA was extracted and sequenced with Roche/454 technology, generating about 3 million reads per sample. To our knowledge this work is the first report of a composting whole-microbial community using high-throughput sequencing and analysis. The phylogenetic profiles of the two microbiomes analyzed are quite different, with a clear dominance of members of the Lactobacillus genus in one of them. We found a general agreement of the distribution of functional categories in the Zoo compost metagenomes compared with seven selected public metagenomes of biomass deconstruction environments, indicating the potential for different bacterial communities to provide alternative mechanisms for the same functional purposes. Our results indicate that biomass degradation in this composting process, including deconstruction of recalcitrant lignocellulose, is fully performed by bacterial enzymes, most likely by members of the Clostridiales and Actinomycetales orders.FAPESP 2009/52030-5RCNPqCAPE