A RECEPTOR FOR ANTIBODY ON B LYMPHOCYTES : I. METHOD OF DETECTION AND FUNCTIONAL SIGNIFICANCE

Evidence is presented for the existence on all B lymphocytes, but not on T lymphocytes, of a membrane-associated receptor for antibody. The receptor was detected by a radioautographic technique in which lymphoid cells were incubated with antibody followed by the corresponding radioiodinated antigen. The ease with which antibody eluted during washing indicated that the bond between antibody and cell was weak. The formation of an antibody-antigen complex on the cell surface, however, stabilized the bond and permitted accurate quantitation of cells with adherent antibody. The ability of several combinations of antibody and antigen to adhere to the cells demonstrated the nonspecificity of the phenomenon and emphasized the need for care in interpretation of antigen-binding studies particularly when immune cells are being used. The identity of antibody-binding lymphocytes was established by two different approaches. In the first, mouse lymphocyte populations greatly enriched for either T cells or B cells were examined. Their T cell content was assessed by means of well-established markers such as the θ C3H isoantigen. When this was compared with the number of antibody-binding cells, an inverse relationship was obtained in each instance; thus almost all thoracic duct cells from athymic mice labeled with an immune complex although none were θ positive. The striking reduction in antibody-binding cells observed in bursectomized chickens provided a second and independent line of evidence suggesting that B cells, not T cells, bind antibody. The ability of B cells from primed animals to bind antibody in vivo made it important to test whether this phenomenon was related to the carriage of immunological memory. No correlation was, however, found between membrane-bound antibody and memory. It was proposed that the existence of a receptor of this kind may provide a rational explanation for antibody-dependent killing of target cells and may prove of importance in antigen concentration particularly during the secondary response

A tool for fast ground truth generation for object detection and tracking from video

Object detection and tracking is one of the most important components in computer vision applications. To carefully evaluate the performance of detection and tracking algorithms, it is important to develop benchmark data sets. One of the most tedious and error-prone aspects when developing benchmarks, is the generation of the ground truth. This paper presents FAST-GT (FAst Semi-automatic Tool for Ground Truth generation), a new generic framework for the semiautomatic generation of ground truths. FAST-GT reduces the need for manual intervention thus speeding-up the ground-truthing process

RASW: A run-time adaptive sliding window to improve Viola-Jones object detection

Abstract—In recent years accurate algorithms for detecting objects in images have been developed. Among these algorithms, the object detection scheme proposed by Viola and Jones gained great popularity, especially after the release of high-quality face classifiers by the OpenCV group. However, as any other slidingwindow based object detector, it is affected by a strong increase in the computational cost as the size of the scene grows. Especially in real-time applications, a search strategy based on a sliding window can be computationally too expensive. In this paper, we propose an efficient approach to adapt at run time the sliding window step size in order to speed-up the detection task without compromising the accuracy. We demonstrate the effectiveness of the proposed Run-time Adaptive Sliding Window (RASW) in improving the performance of Viola-Jones object detection by providing better throughput-accuracy tradeoffs. When comparing our approach with the OpenCV face detection implementation, we obtain up to 2.03x speedup in frames per second without any loss in accuracy

Critical Behavior of Disordered Systems with a Free Surface

The behavior of homogeneous and disordered systems with a free boundary is described on the basis of group theory in the two-loop approximation directly in three-dimensional space. The effect of the free boundary on the regime of the bulk critical behavior is revealed. It is shown that the boundedness of the system slightly affects the regime of the bulk critical behavior in the case of the ordinary transition, whereas this effect is more noticeable in the case of the special transition. Surface critical phenomena are described for homogeneous and disordered systems, and the critical exponents are calculated in the two-loop approximation. It is shown that the effect of impurities is insignificant in the special phase transition, whereas it is more noticeable in the ordinary phase transition. The derived critical exponents are compared with the computer-simulation results.Comment: 19 pages, 2 figure