Organisation en réseau et durabilité systémique de deux filières alimentaires

La durabilité des filières alimentaires est questionnée par les pouvoirs publics et les consommateurs, qui tendent à défendre des formes alternatives de production, respectueuses de l'environnement, économiquement et socialement acceptables. Ces formes sont toutefois confrontées à des freins techniques, économiques et institutionnels d'où un questionnement sur leur capacité à se développer et à se maintenir, et donc sur leur durabilité. A partir d'une étude systémique de deux filières alternatives, le petit épeautre de Haute Provence et le riz biologique de Camargue, nous nous sommes attachés à comprendre comment l'organisation des acteurs peut influer sur les trois piliers du développement durable. Nous exposons une manière de considérer le développement durable qui met en avant les relations entre les acteurs, à l'aide de l'outil de l'analyse de réseau, et des entretiens menés avec un échantillon de différents acteurs des filières. Nous montrons que la volonté de pérennisation de la filière influe sur le choix du type d'organisation au sein de la filière, qui peut être orienté pour plus ou moins favoriser la prise en compte des piliers du développement durable. Nos observations montrent l'importance de considérer les réseaux dans une vision systémique de la durabilité. Il reste toutefois difficile d'établir des relations de causalité entre d'une part le type d'organisation et d'autre part la durabilité

Description of 'Bacillus dakarensis' sp. nov., 'Bacillus sinesaloumensis' sp. nov., 'Gracilibacillus timonensis' sp. nov., 'Halobacillus massiliensis' sp. nov., 'Lentibacillus massiliensis' sp. nov., 'Oceanobacillus senegalensis' sp. nov., 'Oceanobacillus timonensis' sp. nov., 'Virgibacillus dakarensis' sp. nov. and 'Virgibacillus marseillensis' sp. nov., nine halophilic new species isolated from human stool.

International audienceWe report the main characteristics of 'Bacillus dakarensis' P3515(T) sp. nov., 'Bacillus sinesaloumensis' P3516(T) sp. nov., 'Gracilibacillus timonensis' P2481(T) sp. nov., 'Halobacillus massiliensis' P3554(T) sp. nov., 'Lentibacillus massiliensis' P3089(T) sp. nov., 'Oceanobacillus senegalensis' P3587(T) sp. nov., 'Oceanobacillus timonensis' P3532(T) sp. nov., 'Virgibacillus dakarensis' P3469(T) sp. nov. and 'Virgibacillus marseillensis' P3610(T) sp. nov., that were isolated in 2016 from salty stool samples (\textgreater/=1.7% NaCl) from healthy Senegalese living at Dielmo and N'diop, two villages in Senegal

Oceanobacillus timonensis sp. nov. and Oceanobacillus senegalensis sp. nov., two new moderately halophilic, Gram-stain positive bacteria isolated from stools sample of healthy young Senegalese

Oceanobacillus timonensis Marseille-P3532(T) (CSUR P3532, CCUG 70981) and Oceanobacillus senegalensis Marseille-P3587(T) (CSUR P3587, CCUG 70613), are the type strains of O. timonensis sp. nov. and O. senegalensis sp. nov., respectively. They are moderately halophilic, aerobic, motile and Gram-stain positive bacteria. The strains P3532(T) and P3587(T) were isolated from stools with 3.8% and 2.1% sodium chloride (NaCl) of healthy 10 year old female and male 7-year-old children, respectively and living respectively at Dielmo and N'diop two villages in Senegal (West Africa). This study aimed to describe the genome and phenotypic characteristics of O. timonensis Marseille-P3532(T) and O. senegalensis Marseille-P3587(T). The genomes are 4,485,335bp long for O. timonensis and 4,300,331 bp for O. senegalensis with 38.78% and 36.92% G+C content, respectively. They contain 4306 and 3979 protein-coding and 87 and 273 RNAs genes, respectively

Leptospirosis, one neglected disease in rural Senegal

A serological study was carried out in two Senegalese villages located in the Sine-Saloum region in order to estimate the presence of anti-leptospiral antibodies in humans and animals, and to identify the predominant serogroups. Seven hundred and forty-nine serum samples were collected from humans (n = 545), dogs (n = 33), donkeys (n = 20), goats (n = 52), sheep (n = 43) and N'Dama cattle (n = 56), all originated from Dielmo and Ndiop villages. All samples were tested for different serovars of pathogenic Leptospira species by the microscopic agglutination test. Considering titres >= 1:100, 7.7% [CI 95:5.5 to 9.9] on the 545 human blood samples tested and 42.2% [CI95:35.4 to 48.9] on the 204 animal blood samples tested were found to be positive to one or more serovars. The results obtained indicate that the Australis serogroup is the most prevalent serogroup in human (67.3%) and cattle (27.3%). Serogroup Icterohaemorhagiae is the most frequent serogroup in goat (55.6%) and donkey (37.5%). Canicola (23.4%), Icterohaemorhagiae (21.1%) and Australis (12.5%) serogroups are the most prevalent serogroups in dogs. This study shows that diverse Leptospira serovars occur in a wide range of wild and domestic mammal species, as well as in humans in Senegal. However, further studies are needed to better understand the complexity of Leptospira epidemiology in Africa, identify the reservoirs of different serogroups and estimate its impact on livestock. Understanding the multi-host epidemiology of leptospirosis is essential to control and prevent the disease

Non-additive gene regulation in a citrus allotetraploid somatic hybrid between C. reticulata Blanco and C. limon (L.) Burm

Polyploid plants often produce new phenotypes, exceeding the range of variability existing in the diploid gene pool. Several hundred citrus allotetraploid hybrids have been created by somatic hybridization. These genotypes are interesting models to study the immediate effects of allopolyploidization on the regulation of gene expression. Here, we report genome-wide gene expression analysis in fruit pulp of a Citrus interspecific somatic allotetraploid between C. reticulata cv ‘Willowleaf mandarin’+C. limon cv ‘Eureka lemon’, using a Citrus 20K cDNA microarray. Around 4% transcriptome divergence was observed between the two parental species, and 212 and 160 genes were more highly expressed in C. reticulata and C. limon, respectively. Differential expression of certain genes was confirmed by quantitative real-time RT-PCR. A global downregulation of the allotetraploid hybrid transcriptome was observed, as compared with a theoretical mid parent, for the genes displaying interspecific expression divergence between C. reticulata and C. limon. The genes underexpressed in mandarin, as compared with lemon, were also systematically repressed in the allotetraploid. When genes were overexpressed in C. reticulata compared with C. limon, the distribution of allotetraploid gene expression was far more balanced. Cluster analysis on the basis of gene expression clearly indicated the hybrid was much closer to C. reticulata than to C. limon. These results suggest there is a global dominance of the mandarin transcriptome, in consistence with our previous studies on aromatic compounds and proteomics. Interspecific differentiation of gene expression and non-additive gene regulation involved various biological pathways and different cellular components

Quality of sterile male tsetse after long distance transport as chilled, irradiated pupae

Background Tsetse flies transmit trypanosomes that cause human and African animal trypanosomosis, a debilitating disease of humans (sleeping sickness) and livestock (nagana). An area-wide integrated pest management campaign against Glossina palpalis gambiensis has been implemented in Senegal since 2010 that includes a sterile insect technique (SIT) component. The SIT can only be successful when the sterile males that are destined for release have a flight ability, survival and competitiveness that are as close as possible to that of their wild male counterparts..Methodology/Principal Findings Tests were developed to assess the quality of G.p. gambiensis males that emerged from pupae that were produced and irradiated in Burkina Faso and Slovakia (irradiation done in Seibersdorf, Austria) and transported weekly under chilled conditions to Dakar, Senegal. For each consignment a sample of 50 pupae was used for a quality control test (QC group). To assess flight ability, the pupae were put in a cylinder filtering emerged flies that were able to escape the cylinder. The survival of these flyers was thereafter monitored under stress conditions (without feeding). Remaining pupae were emerged and released in the target area of the eradication programme (RF group). The following parameter values were obtained for the QC flies: average emergence rate more than 69%, median survival of 6 days, and average flight ability of more than 35%. The quality protocol was a good proxy of fly quality, explaining a large part of the variances of the examined parameters.Conclusions/Significance The quality protocol described here will allow the accurate monitoring of the quality of shipped sterile male tsetse used in operational eradication programmes in the framework of the Pan-African Tsetse and Trypanosomosis Eradication Campaign

Common epidemiology of Rickettsia felis infection and malaria, Africa

This study aimed to compare the epidemiology of Rickettsia felis infection and malaria in France, North Africa, and sub-Saharan Africa and to identify a common vector. Blood specimens from 3,122 febrile patients and from 500 nonfebrile persons were analyzed for R. felis and Plasmodium spp. We observed a significant linear trend (p<0.0001) of increasing risk for R. felis infection. The risks were lowest in France, Tunisia, and Algeria (1%), and highest in rural Senegal (15%). Co-infections with R. felis and Plasmodium spp. and occurrences of R. felis relapses or reinfections were identified. This study demonstrates a correlation between malaria and R. felis infection regarding geographic distribution, seasonality, asymptomatic infections, and a potential vector. R. felis infection should be suspected in these geographical areas where malaria is endemic. Doxycycline chemoprophylaxis against malaria in travelers to sub-Saharan Africa also protects against rickettsioses; thus, empirical treatment strategies for febrile illness for travelers and residents in sub-Saharan Africa may require reevaluation