Ertüchtigung der Flachschleifmaschine ELB SF 30

Aufbauend auf dem Praktikumsbericht wird in der vorliegenden Bachelorarbeit, das Thema „Ertüchtigung der Flachschleifmaschine ELB“ weiter behandelt. Es wird dabei speziell die Einführung eines neuen Bedienpanels an der Maschine konkretisiert. Zu Beginn erfolgt die Auswahl eines geeigneten Panels, welches zu der vorhandenen Steuerung kompatibel ist. Daraufhin folgt die Vorstellung der Visualisierungssoftware, sowie der Software zum Testen der projektierten Oberfläche des Bediengerätes. Im weiteren Verlauf finden sich Erläuterungen zur Programmierung des Panels, bezogen auf das ELB-Projekt wieder. Das letzte Kapitel bezieht sich auf die konkrete SPS-Lösung zwei ausgewählter Steuerungsfunktionen, zum einen die Magnetsteuerung und zum anderen die Querantriebsteuerung

CHANGES IN PATTERNS OF ALKALINE SERINE PROTEASE AND BACILYSIN FORMATION CAUSED BY COMMON EFFECTORS OF SPORULATION IN BACILLUS-SUBTILIS-168

Bacilysin biosynthesis and alkaline serine protease production in Bacillus subtilis 168 were monitored and compared in batch cultures when various effectors of sporulation were added at different stages of growth in a medium containing sucrose and glutamate. Depending on the time of addition, glucose affected sporulation and serine protease formation to the same extent, but had no effect on bacilysin production. Ammonium and L-alanine additions suppressed all three processes. Casamino acids severely interfered with bacilysin formation and sporulation, but not with protease formation. Decoyinine, a well-known inducer of sporulation, induced protease formation as well, but did not affect bacilysin biosynthesis. The extent of the observed effects depended largely on the time of metabolite additions. The results are discussed with reference to a possible coregulation of sporulation and the formation of bacilysin and alkaline serine protease in B. subtilis

Immune response to 17 beta-estradiol involved in polymer gels: Antigen specificity and affinity of hybridoma clones

The immunogenic properties of 17 beta -estradiol, immobilized in negatively charged polymer gels, were investigated, and the specificity of antibodies produced was analyzed. The polymer gels developed were composed of a hydrophobic estradiol core surrounded by hydrophilic polyanions as corona. As an immunogen, it was conceived to function via a dual mode, that is as a hapten-delivery system (prolongation effect) and as a polyelectrolyte adjuvant. Polymer gels containing estradiol appeared to possess a high estradiol-specific immunogenicity even without the addition of traditional adjuvants. A comparative study of estradiol trapped in polymer gels versus estradiol conjugated to bovine serum albumin (BSA.E) + Incomplete Freund's Adjuvant (IFA) mixtures revealed similar immunogenic properties in terms of induction of specific antibodies. Following a short immunization procedure based on the use of 17 beta -estradiol immobilized in polymer gels, we developed 10 specific monoclonal antibodies with K-d values ranging between 1.2 X 10(-7) and 8 X 10(-8) M

Immune response to 17 beta-estradiol in polyelectrolyte complex: Antigen specificity and affinity of hybridoma clones

Complex formation between synthetic polyelectrolytes (PE) [poly-4-vinyl-N-ethyl (cetyl), pyridine bromides-PVP(R(2),R(16))], bovine serum albumin (BSA), or 17 beta-estradiol-BSA conjugate (BSA.E) was studied in neutral water, Weakly water-soluble (colloidal) complex was formed upon addition of BSA.E to PVP (R(2),R(16)) solution at pH 7, A nonrandom distribution of the protein molecules between the coils of polycations and self-assembly in the nonstochiometric polycomplex particles took place, The immunogenic properties of PVP (R(2),R(16))-BSA.E polycomplex were investigated and the specificities of produced antibodies analyzed, 17 beta-Estradiol introduced in polyelectrolyte complexes (PE-BSA) was found to invoke considerable increases in the steroid-specific immunoresponse, However, a comparative study of immunogenic activity of polycomplexes versus BSA.E + incomplete Freund's adjuvant (IFA) mixtures revealed some differences in regards to the specificity of antibody production, In contrast to IFA + BSA.E systems, polycomplexes were able to generate estradiol-as well as BSA-specific antibodies, Such a carrier-directed response may be determined by increase in inmunogenicity of weak antigenic determinants and/or by the exposure of internally located determinants upon complex formation with polyelectrolytes, Fusions following the two different immunization procedures resulted in the growth of comparable numbers of estradiol-specific monoclonal antibodies with apparently similar antigen affinities, Thus, immunizations using antigens in PEC appear to provide an efficient alternative to IFA

New amphiphilic immunogens by poly(N-isopropylacrylamide)-modified bovine serum albumin

Using poly (N-isopropylacrylamide) co-oligomer with N,N-dimethylacrylamide (DMAAm) (IDc) we prepared polymer-protein conjugates of bovine serum albumin (IDc-BSA). The conjugate underwent reversible hydration-dehydration changes in response to temperature changes and revealed phase separation at body temperature. Mice were immunized by the intravenous, intramuscular or intradermal routes with the IDc-BSA. The intravenous route of single immunization without adjuvants evoked increased primary and secondary specific immune responses to IDc-BSA, whereas the intramuscular and intradermal did not elicit higher antibody production. The intradermal administration of BSA and IDc-BSA together with IFA both gave rise to high immunological activity. It is suggested that the hydrophobic chain aggregation site of the IDc-BSA conjugate at body temperature would increase the adsorptive capacity of BSA on the immunocompetent cells. The efficiency of such ''forced'' interactions of conjugate aggregates (high epitope density) are high enough for the immune response. Copyright (C) 1996 Published by Elsevier Science Lt