An investigation on fish parasites in Hanna Wetland, Semirom, Isfahan Province

As a part of a major ecological study of Hanna Wetland, Semirum Region, Isfahan Province, parasitic infestation of 3 native and one introduced fish species were investigated. A total of 120 fish specimens were collected by both cast net and a series of gillnets between fall and winter 2007, and spring and summer 2008. Some of the observed parasites are reported for the first time as new host records of: Trichodina perforata on gill and skin of Carassius auratus auratus and three species of leeches namely: Glossiphonia heteroclite, Thromyzon tessulatum and Hemiclepsis marginata on the skin and fin bases of Capoeta damascina. Moreover, 14 internal and external parasites including: one protozoan; Ichthyophthirius multifiliis and two myxosporeans; Myxobolus varicorhini, Myxobolus sp., three monogeneans; Dactylogyrus lenkoran, Dactylogyrus intermedius and Gyrodactylus sp., two immature digeneans; Diplostomum spathaceum, Tylodephys sp., two mature digeneans; Allocreadium isoporum and A. layman, one unknown cyst, one Acanthocephalan; Acanthocephalorhynchoides sp., one cestoda; Khawia armeniaca. The highest prevalence of Diplostomum spathaceum (100%) was observed in Aphanius isfahanensis and Alburnus sp. However, the lowest prevalence was related to A. layman in Capoeta damascina in winter (0%), and autumn and spring (11%). Leeches infections were only observed in Capoeta damascina and Alburnus sp. in winter and summer, respectively. The maximum average (±SD) infection intensity belonged to K. armeniaca in intestine of C. damascina in spring (12.50±3.40, range: 8-16). Among the parasites identified in the region, D. spathaceum showed the highest mean (±SD) abundance (41.67±11.79) in C. auratus auratus in spring. In general, a relatively high diversity of fish parasitic fauna exists in this water body which could jeopardize fish populations and the whole ecosystem if the ecological status of the Hanna Wetland is neglected

Production of Sn02 nano-particles by hydrogel thermal decomposition method

SnO2 is an important functional material having a wide range of applications in gas sensors and optoelectronic devices. There is a great interest for finding new costeffective and straight-forward methods for production of these particles. In this research, hydrogel thermal decomposition method (HTDM) is used for production of high purity SnO2nano-particles. Cost effective reactants and green routs of production are the advantages of polysaccharide based hydrogel as starting material for this method. Visual observations indicated that there is very little tendency for agglomeration in the SnO2nano-particles produced by this method which can be considered as an advantage for this method over other methods for production of SnO2nano-particles. SnO2nanoparticles are also characterized by X-ray diffraction (XRD) in terms of purity and the sizes. It is found that high purity SnO2nano-particles in the size range of 25 – 36 nm can be produced by HTDM. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/2068

The association between menarche age and first offspring sex ratio

Background: Offspring sex ratio (OSR) serves as an important social factor, and various other factors are hypothesized to be associated with it, such as maternal diet, time of ovulation and insemination, environmental phenomena, parental age, and infertility treatment. Objectives: This research was performed to assess the association between mothers� menarche age, first pregnancy age, and sex ratio of first offspring. Patients and Methods: In this retrospective study, 2,000 Iranian women of reproductive age were recruited to assess their menarche age, first pregnancy age, and first OSR. Results: The mean age of the participants was 29.6 ± 7.09, their mean menarche age was 13.25 ± 1.21, and the mean age of their first pregnancy was 23.9 ± 4.35. Total OSR was 0.932. The OSR (calculated as the proportion of male to female offspring) was higher when the menarche age was younger (P < 0.05). In women of younger pregnancy age, the OSR was higher (P < 0.05). First pregnancy age was younger in women with a menarche age under 13 years (P < 0.05). Conclusions: Women of younger menarche age will have younger first pregnancy ages and a greater chance of having a male first offspring. © 2016, Shiraz University of Medical Sciences

Yield, fruit quality and physiological responses of melon cv. Khatooni under deficit irrigation

To evaluate the effect of water deficit stress on growth, yield, fruit quality and physiological traits of melon cv. Khatooni, field experiments were conducted in split plot randomized complete block design with three replications. In 2014, irrigation treatments consisted of two deficit irrigation regimes, 33% and 66% of ETc (crop evapotranspiration), and 100% ETc as the control (DI33, DI66 and I100). In 2015, irrigation treatments applied were: 40, 70 and 100% ETc (DI40, DI70 and I100). The results showed that plant height and leaf area decreased from treatment I100 to DI40 and DI33. The highest average fruit weigh and yield were obtained from irrigation 100% ETc for both years. The water use efficiency (WUE) significantly increased in response to increase water deficit stress. Deficit irrigation treatments significantly decreased leaf relative water content, vitamin C and fruit firmness, whereas antioxidant enzymes activity, proline and total soluble solid contents increased. These results suggest that the crop is sensitive to water deficits, that moderate water stress (DI70 and DI66) reduced yield by about 28.5-38.2% and severe water stress (DI40 and DI33) had a much more marked effect, reducing yield by 48.1-61.4%

The modalities of Iranian soft power: from cultural diplomacy to soft war

Through exploring Iran's public diplomacy at the international level, this article demonstrates how the Islamic Republic's motives should not only be contextualised within the oft-sensationalised, material or ‘hard’ aspects of its foreign policy, but also within the desire to project its cultural reach through ‘softer’ means. Iran's utilisation of culturally defined foreign policy objectives and actions demonstrates its understanding of soft power's potentialities. This article explores the ways in which Iran's public diplomacy is used to promote its soft power and craft its, at times, shifting image on the world stage

Assessing mastery through psychotherapy: Psychometric properties of the persian version of the mastery scale

Background: Considering the importance of assessing the range of phenomena that can change in psychodynamic therapy, and lack of appropriate assessment scale in Persian language, the present study assessed the reliability of the Persian mastery scale. The mastery scale is a comprehensive research tool for assessing a patient's mastery of transference-related interpersonal conflicts during the treatment process. Objectives: The aim of this research was to examine the psychometric properties of the Persian version of the Mastery Scale. Methods: After preparing the Persian version of the mastery scale through the forward-backward translation process, a sample of 36 relationship episodes was rated by three independent judges. Data were collected in Mashhad, Iran in 2015. The psychometric properties of the instrument, including inter-rater reliability and test-retest analysis were assessed. Results: The test-retest correlations of the 3 judges were (0.80, 0.91 and 0.95), highly significant at P < 0.0001 and the inter-rater reliability indicated a good level of agreement (The ICC (3, 3) 0.84, 95 CI 0.72, 0.90). Conclusions: The current study has provided evidence that the Persian mastery scale is a reliable instrument for assessing changes in inner capacities and resources through psychodynamic treatments. However, further studies are required to investigate the validity of this scale. ©2018, Iranian Journal of Psychiatry and Behavioral Sciences

A Novel Combination of Surfactant Addition and Persulfate-assisted Electrokinetic Oxidation for Remediation of Pyrene-Contaminated Soil

Effect of surfactant addition on persulfate-assisted electrokinetic remediation of pyrene-spiked soil was studied. The influence of effective factors including voltage, surfactant addition, moisture content, and persulfate concentration on the removal of initial pyrene concentration of 200 mg kg–1 were investigated. A complete pyrene removal was observed for voltage of 1 V cm–1, saturated conditions, Tween 80 concentration of 20 mL kg–1, and persulfate concentration of 100 mg kg–1 after 24 h, corresponding to pyrene mineralization of 61 %, based on TPH analysis. The experimental results were best fitted with pseudo-first-order kinetic model with correlation coefficient of 0.968 and rate constant of 0.191 min−1. The main intermediates of pyrene degradation were benzene o-toluic acid, acetic, azulene, naphthalene and decanoic acid. Finally, an unwashed hydrocarbon- contaminated soil was subjected to persulfate-assisted electrokinetic remediation, and a TPH removal of 38 % was observed for the initial TPH content of 912 mg kg–1, under the selected conditions. This work is licensed under a Creative Commons Attribution 4.0 International License

Biological activities of fusarochromanone: a potent anti-cancer agent

Background Fusarochromanone (FC101) is a small molecule fungal metabolite with a host of interesting biological functions, including very potent anti-angiogenic and direct anti-cancer activity. Results Herein, we report that FC101 exhibits very potent in-vitro growth inhibitory effects (IC50 ranging from 10nM-2.5 μM) against HaCat (pre-malignant skin), P9-WT (malignant skin), MCF-7 (low malignant breast), MDA-231 (malignant breast), SV-HUC (premalignant bladder), UM-UC14 (malignant bladder), and PC3 (malignant prostate) in a time-course and dose-dependent manner, with the UM-UC14 cells being the most sensitive. FC101 induces apoptosis and an increase in proportion of cells in the sub-G1 phase in both HaCat and P9-WT cell lines as evidenced by cell cycle profile analysis. In a mouse xenograft SCC tumor model, FC101 was well tolerated, non-toxic, and achieved a 30% reduction in tumor size at a dose of 8 mg/kg/day. FC101 is also a potent anti-angiogenenic agent. At nanomolar doses, FC101 inhibits the vascular endothelial growth factor-A (VEGF-A)-mediated proliferation of endothelial cells. Conclusions Our data presented here indicates that FC101 is an excellent lead candidate for a small molecule anti-cancer agent that simultaneously affects angiogenesis signaling, cancer signal transduction, and apoptosis. Further understanding of the underlying FC101’s molecular mechanism may lead to the design of novel targeted and selective therapeutics, both of which are pursued targets in cancer drug discovery

EVER Proteins, Key Elements of the Natural Anti-Human Papillomavirus Barrier, Are Regulated upon T-Cell Activation

Human papillomaviruses (HPV) cause a variety of mucosal and skin lesions ranging from benign proliferations to invasive carcinomas. The clinical manifestations of infection are determined by host-related factors that define the natural anti-HPV barrier. Key elements of this barrier are the EVER1 and EVER2 proteins, as deficiency in either one of the EVER proteins leads to Epidermodysplasia Verruciformis (EV), a genodermatosis associated with HPV-induced skin carcinoma. Although EVERs have been shown to regulate zinc homeostasis in keratinocytes, their expression and function in other cell types that may participate to the anti-HPV barrier remain to be investigated. In this work, we demonstrate that EVER genes are expressed in different tissues, and most notably in lymphocytes. Interestingly, in contrast to the skin, where EVER2 transcripts are hardly detectable, EVER genes are both abundantly expressed in murine and human T cells. Activation of CD4+ and CD8+ T cells via the TCR triggers a rapid and profound decrease in EVER expression, accompanied by an accumulation of free Zn2+ ions. Thus, EVER proteins may be involved in the regulation of cellular zinc homeostasis in lymphocytes. Consistent with this hypothesis, we show that the concentration of Zn2+ ions is elevated in lymphoblastoid cells or primary T cells from EVER2-deficient patients. Interestingly, we also show that Zn2+ excess blocks T-cell activation and proliferation. Therefore, EVER proteins appear as key components of the activation-dependent regulation of Zn2+ concentration in T cells. However, the impact of EVER-deficiency in T cells on EV pathogenesis remains to be elucidated

Whole genome sequencing reveals a 7 base-pair deletion in DMD exon 42 in a dog with muscular dystrophy

Dystrophin is a key cytoskeletal protein coded by the Duchenne muscular dystrophy (DMD) gene located on the X-chromosome. Truncating mutations in the DMD gene cause loss of dystrophin and the classical DMD clinical syndrome. Spontaneous DMD gene mutations and associated phenotypes occur in several other species. The mdx mouse model and the golden retriever muscular dystrophy (GRMD) canine model have been used extensively to study DMD disease pathogenesis and show efficacy and side effects of putative treatments. Certain DMD gene mutations in high-risk, the so-called hot spot areas can be particularly helpful in modeling molecular therapies. Identification of specific mutations has been greatly enhanced by new genomic methods. Whole genome, next generation sequencing (WGS) has been recently used to define DMD patient mutations, but has not been used in dystrophic dogs. A dystrophin-deficient Cavalier King Charles Spaniel (CKCS) dog was evaluated at the functional, histopathological, biochemical, and molecular level. The affected dog’s phenotype was compared to the previously reported canine dystrophinopathies. WGS was then used to detect a 7 base pair deletion in DMD exon 42 (c.6051-6057delTCTCAAT mRNA), predicting a frameshift in gene transcription and truncation of dystrophin protein translation. The deletion was confirmed with conventional PCR and Sanger sequencing. This mutation is in a secondary DMD gene hotspot area distinct from the one identified earlier at the 5′ donor splice site of intron 50 in the CKCS breed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00335-016-9675-2) contains supplementary material, which is available to authorized users