Constraints on the broad line region from regularized linear inversion: Velocity-delay maps for five nearby active galactic nuclei

Reverberation mapping probes the structure of the broad emission-line region (BLR) in active galactic nuclei (AGN). The kinematics of the BLR gas can be used to measure the mass of the central supermassive black hole. The main uncertainty affecting black hole mass determinations is the structure of the BLR. We present a new method for reverberation mapping based on regularized linear inversion (RLI) that includes modelling of the AGN continuum light curves. This enables fast calculation of velocity-resolved response maps to constrain BLR structure. RLI allows for negative response, such as when some areas of the BLR respond in inverse proportion to a change in ionizing continuum luminosity. We present time delays, integrated response functions, and velocity-delay maps for the $\rm{H}\,\beta$ broad emission line in five nearby AGN, as well as for $\rm{H}\,\alpha$ and $\rm{H}\,\gamma$ in Arp 151, using data from the Lick AGN Monitoring Project 2008. We find indications of prompt response in three of the objects (Arp 151, NGC 5548 and SBS 1116+583A) with additional prompt response in the red wing of $\rm{H}\,\beta$. In SBS 1116+583A we find evidence for a multimodal broad prompt response followed by a second narrow response at 10 days. We find no clear indications of negative response. The results are complementary to, and consistent with, other methods such as cross correlation, maximum entropy and dynamical modelling. Regularized linear inversion with continuum light curve modelling provides a fast, complementary method for velocity-resolved reverberation mapping and is suitable for use on large datasets.Comment: 20 pages, 13 figures, accepted to MNRA

Modeling reverberation mapping data II: dynamical modeling of the Lick AGN Monitoring Project 2008 dataset

We present dynamical modeling of the broad line region (BLR) for a sample of five Seyfert 1 galaxies using reverberation mapping data taken by the Lick AGN Monitoring Project in 2008. By modeling the AGN continuum light curve and H$\beta$ line profiles directly we are able to constrain the geometry and kinematics of the BLR and make a measurement of the black hole mass that does not depend upon the virial factor, $f$, needed in traditional reverberation mapping analysis. We find that the geometry of the BLR is generally a thick disk viewed close to face-on. While the H$\beta$ emission is found to come preferentially from the far side of the BLR, the mean size of the BLR is consistent with the lags measured with cross-correlation analysis. The BLR kinematics are found to be consistent with either inflowing motions or elliptical orbits, often with some combination of the two. We measure black hole masses of $\log_{10}(M_{\rm\,BH}/M_\odot)=6.62^{+0.10}_{-0.13}$ for Arp 151, $7.42^{+0.26}_{-0.27}$ for Mrk 1310, $7.51^{+0.23}_{-0.14}$ for NGC 5548, $6.42^{+0.24}_{-0.18}$ for NGC 6814, and $6.99^{+0.32}_{-0.25}$ for SBS 1116+583A. The $f$ factors measured individually for each AGN are found to correlate with inclination angle, although not with $M_{\rm\,BH}$, $L_{5100}$, or FWHM/$\sigma$ of the emission line profile.Comment: 21 pages, 24 figures, 3 tables, Accepted for publication in MNRAS, corrected masses for NGC 5548 and NGC 6814 in the abstrac

Etablierung DNA-basierender Nanostrukturen aus DNA-Tetraedern über Kissing-Loop-Annealing

In dieser Arbeit konnten auf DNA basierende Nanotetraeder durch bis zu vier DNA-Hairpin-Strukturen an den Tetraederecken funktionalisiert werden. Durch geschicktes Design der Loop-Sequenzen konnte gezeigt werden, dass die funktionalisierten DNA-Tetraeder mittels dieser Sequenzen, über sogenanntes Kissing-Loop-Annealing, miteinander interagieren können. Dieses Kissing-Loop-Annealing wurde in dieser Arbeit im Hinblick auf verschiedene Einflussfaktoren detailliert charakterisiert

Prospects for measuring supermassive black hole masses with future extremely large telescopes

The next generation of giant-segmented mirror telescopes ($>$ 20 m) will enable us to observe galactic nuclei at much higher angular resolution and sensitivity than ever before. These capabilities will introduce a revolutionary shift in our understanding of the origin and evolution of supermassive black holes by enabling more precise black hole mass measurements in a mass range that is unreachable today. We present simulations and predictions of the observations of nuclei that will be made with the Thirty Meter Telescope (TMT) and the adaptive optics assisted integral-field spectrograph IRIS, which is capable of diffraction-limited spectroscopy from $Z$ band (0.9 $\mu$m) to $K$ band (2.2 $\mu$m). These simulations, for the first time, use realistic values for the sky, telescope, adaptive optics system, and instrument, to determine the expected signal-to-noise ratio of a range of possible targets spanning intermediate mass black holes of $\sim10^4$ \msun to the most massive black holes known today of $>10^{10}$ $M_\odot$. We find that IRIS will be able to observe Milky Way-mass black holes out the distance of the Virgo cluster, and will allow us to observe many more brightest cluster galaxies where the most massive black holes are thought to reside. We also evaluate how well the kinematic moments of the velocity distributions can be constrained at the different spectral resolutions and plate scales designed for IRIS. We find that a spectral resolution of $\sim8000$ will be necessary to measure the masses of intermediate mass black holes. By simulating the observations of galaxies found in SDSS DR7, we find that over $10^5$ massive black holes will be observable at distances between $0.005 < z < 0.18$ with the estimated sensitivity and angular resolution provided by access to $Z$-band (0.9 $\mu$m) spectroscopy from IRIS and the TMT adaptive optics system. (Abridged)Comment: 19 pages, 20 figures, accepted to A

High-yield production of a super-soluble miniature spidroin for biomimetic high-performance materials

The mechanical properties of artificial spider silks are approaching a stage where commercial applications become realistic. However, the yields of recombinant silk proteins that can be used to produce fibers with good mechanical properties are typically very low and many purification and spinning protocols still require the use of urea, hexafluoroisopropanol, and/or methanol. Thus, improved production and spinning methods with a minimal environmental impact are needed. We have previously developed a miniature spider silk protein that is characterized by high solubility in aqueous buffers and spinnability in biomimetic set-ups. In this study, we developed a production protocol that resulted in an expression level of >20 g target protein per liter in an Escherichia coli fedbatch culture, and subsequent purification under native conditions yielded 14.5 g/l. This corresponds to a nearly six-fold increase in expression levels, and a 10-fold increase in yield after purification compared to reports for recombinant spider silk proteins. Biomimetic spinning using only aqueous buffers resulted in fibers with a toughness modulus of 74 MJ/m(3), which is the highest reported for biomimetically as-spun artificial silk fibers. Thus, the process described herein represents a milestone for the economic production of biomimetic silk fibers for industrial applications

DNA-DNA kissing complexes as a new tool for the assembly of DNA nanostructures

Kissing-loop annealing of nucleic acids occurs in nature in several viruses and in prokaryotic replication, among other circumstances. Nucleobases of two nucleic acid strands (loops) interact with each other, although the two strands cannot wrap around each other completely because of the adjacent doublestranded regions (stems). In this study, we exploited DNA kissing-loop interaction for nanotechnological application. We functionalized the vertices of DNA tetrahedrons with DNA stem-loop sequences. The complementary loop sequence design allowed the hybridization of different tetrahedrons via kissingloop interaction, which might be further exploited for nanotechnology applications like cargo transport and logical elements. Importantly, we were able to manipulate the stability of those kissing-loop complexes based on the choice and concentration of cations, the temperature and the number of complementary loops per tetrahedron either at the same or at different vertices. Moreover, variations in loop sequences allowed the characterization of necessary sequences within the loop as well as additional stability control of the kissing complexes. Therefore, the properties of the presented nanostructures make them an important tool for DNA nanotechnology