Jensen, Marlene Mark

Mutlu, A. Gizem

Smets, Barth F.

Vangsgaard, Anna Katrine

Online Research Database In Technology

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Gizem; Vangsgaard, Anna Katrine; Jensen, Marlene Mark; Smets, Barth F.Publication date:2012Link back to DTU OrbitCitation (APA):Mutlu, A. G., Vangsgaard, A. K., Jensen, M. M., & Smets, B. F. (2012). Architecture evolution of biomassaggregates in single stage nitritation/anammox reactors. Poster session presented at 14th InternationalSymposium on Microbial Ecology, Copenhagen, Denmark.• Completely autotrophic nitrogen removal (CANR) from wastewater in a single reactor requires simultaneous presence of aerobic and anaerobic ammonium oxidizing bacteria, AOB and AnAOB, performing nitritation and anammox, respectively.  • For CANR systems, aggregation is a common concept, providing favourable conditions for self-immobilizing these slow-growing microorganisms within their required redox microenvironments. • Different biomass architectures are observed in different systems, and not necessarily with spatial segregation of the two guilds via stratification. Single-stage nitritation/anammox CANR  • Increasing substrate loading rate under intermittent operation lead to aggregation of floccular CANR biomass.  • Biomass develops into 2 distinct size groups, mainly of small AOB and large AnAOB homo-aggregates. • Compositions are governed by time-segregation of redox conditions and not spatial-segregation (stratification). Architecture evolution of biomass aggregates in  single-stage nitritation/anammox reactors A. Gizem Mutlu1, A. Katrine Vangsgaard2, Marlene M. Jensen1, Barth F. Smets1                     agzm@env.dtu.dk Particle size distribution w.r.t.  Substrate loading rate Conclusions Day  134 Day  147 Day  193 Day  255 Day  288 Day  453 qFISH w.r.t. Particle size distribution Questions on CANR aggregation Lab-scale (4L) sequencing batch reactor (SBR) performing single-stage nitritation/anammox with intermittent aeration • Ammonium loading rate is increased from 300 gNH4+-N/m3.d to 750 gNH4+-N/m3.d keeping overall O2/NH4+-N loading the same over approx. 150 days. • Increased TN removal rate is accompanied by increasing volume fraction of particles dp<40µm and dp>200µm • At constant high substrate loading rates, the volume fraction of lower size particles dominate the system  Upon increasing substrate loading rate: •Particle size distribution converts to a bimodal curve with peaks at 13µm and 120µm •Two peaks correspond to two major aggregate formations Flocs to Aggregates Timeline Before increasing substrate loading:   Floccular biomass of mixed AOB AnAOB microcolonies and single cells Upon increasing substrate loading:   Compact red AnAOB aggregates of dp>40 µm, some with diffuse outer layer of AOB start to form  Further increase in substrate loading:   Red AnAOB aggregates with dp>40 µm grow larger up to 700µm, with no clear stratification; whereas a separate group of  AOB based  white aggregates of dp<40µm develop AOB AnAOB EUB AOBmix - Cy3 Nmo218 Nso190 Cluster 6a192  + Competitor AnAOB – Cy5 Amx820 EUBmix - FLUOS EUB 338 I EUB 338 II EUB 338 III  0          µm        400  0          µm        400  0          µm        400 AOB AnAOB NOB AOB AOB AnAOB NOB Flocs developing into big red and small white aggregates This project is funded by Danish Science Foundation (DSF) project EcoDesign MBR  Bulk particle size distribution measured with laser diffractometer over time 0 100 200 300 400 500 600 700 0 10 20 30 40 50 60 70 80 90 100 134 147 195 257 271 285 293 313 327 341 355 369 383 404 418 453 TN Removal Rate (gN/m3.d) % Volume Fraction Time of Operation (days) dp<40µm 40µm<dp<200µm dp>200µm TN removal rate X  vol. fraction dp < 40µm X  vol. fraction dp > 40µm Estimated total microbial community distribution qFISH reveals: • 13µm peak dominated by AOB microcolonies • 120µm peak  dominated by AnAOB aggregates • This bimodal homo-aggregation is the base for steering the architecture towards compact stratified hetero-aggregation for robust nitritation/anammox microbial community performing at higher treatment efficiencies. • The CANR system can be managed by exploiting the operational conditions from time-segregated for homo-aggregates to spatially-segregated for hetero-aggregates. Outlook Day 1Department of Environmental Engineering, Technical University of Denmark 2Department of Chemical and Biochemical Engineering, Technical University of Denmark  How can we control/impose aggregate architecture in CANR systems?  • Does increasing substrate loading under intermittent operation, through stringent feast/famine conditions, impose aggregation? • Is aggregation necessarily accompanied by spatial stratification?    How does the aggregation architecture evolve in CANR systems?  • What type of aggregates develop?  • What are their physical characteristics? • What is their microbial community structure?  heterotrophic denitrifying bacteria?  0          µm        400  0          µm        400  0          µm        400 

Architecture evolution of biomass aggregates in single stage nitritation/anammox reactors

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Architecture evolution of biomass aggregates in single stage nitritation/anammox reactors

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