Assembly and budding of influenza virus.

Influenza viruses are causative agents of an acute febrile respiratory disease called influenza (commonly known as "flu") and belong to the Orthomyxoviridae family. These viruses possess segmented, negative stranded RNA genomes (vRNA) and are enveloped, usually spherical and bud from the plasma membrane (more specifically, the apical plasma membrane of polarized epithelial cells). Complete virus particles, therefore, are not found inside infected cells. Virus particles consist of three major subviral components, namely the viral envelope, matrix protein (M1), and core (viral ribonucleocapsid [vRNP]). The viral envelope surrounding the vRNP consists of a lipid bilayer containing spikes composed of viral glycoproteins (HA, NA, and M2) on the outer side and M1 on the inner side. Viral lipids, derived from the host plasma membrane, are selectively enriched in cholesterol and glycosphingolipids. M1 forms the bridge between the viral envelope and the core. The viral core consists of helical vRNP containing vRNA (minus strand) and NP along with minor amounts of NEP and polymerase complex (PA, PB1, and PB2). For viral morphogenesis to occur, all three viral components, namely the viral envelope (containing lipids and transmembrane proteins), M1, and the vRNP must be brought to the assembly site, i.e. the apical plasma membrane in polarized epithelial cells. Finally, buds must be formed at the assembly site and virus particles released with the closure of buds. Transmembrane viral proteins are transported to the assembly site on the plasma membrane via the exocytic pathway. Both HA and NA possess apical sorting signals and use lipid rafts for cell surface transport and apical sorting. These lipid rafts are enriched in cholesterol, glycosphingolipids and are relatively resistant to neutral detergent extraction at low temperature. M1 is synthesized on free cytosolic polyribosomes. vRNPs are made inside the host nucleus and are exported into the cytoplasm through the nuclear pore with the help of M1 and NEP. How M1 and vRNPs are directed to the assembly site on the plasma membrane remains unclear. The likely possibilities are that they use a piggy-back mechanism on viral glycoproteins or cytoskeletal elements. Alternatively, they may possess apical determinants or diffuse to the assembly site, or a combination of these pathways. Interactions of M1 with M1, M1 with vRNP, and M1 with HA and NA facilitate concentration of viral components and exclusion of host proteins from the budding site. M1 interacts with the cytoplasmic tail (CT) and transmembrane domain (TMD) of glycoproteins, and thereby functions as a bridge between the viral envelope and vRNP. Lipid rafts function as microdomains for concentrating viral glycoproteins and may serve as a platform for virus budding. Virus bud formation requires membrane bending at the budding site. A combination of factors including concentration of and interaction among viral components, increased viscosity and asymmetry of the lipid bilayer of the lipid raft as well as pulling and pushing forces of viral and host components are likely to cause outward curvature of the plasma membrane at the assembly site leading to bud formation. Eventually, virus release requires completion of the bud due to fusion of the apposing membranes, leading to the closure of the bud, separation of the virus particle from the host plasma membrane and release of the virus particle into the extracellular environment. Among the viral components, M1 contains an L domain motif and plays a critical role in budding. Bud completion requires not only viral components but also host components. However, how host components facilitate bud completion remains unclear. In addition to bud completion, influenza virus requires NA to release virus particles from sialic acid residues on the cell surface and spread from cell to cell. Elucidation of both viral and host factors involved in viral morphogenesis and budding may lead to the development of drugs interfering with the steps of viral morphogenesis and in disease progression

Gaseous Planets, Protostars And Young Brown Dwarfs : Birth And Fate

We review recent theoretical progress aimed at understanding the formation and the early stages of evolution of giant planets, low-mass stars and brown dwarfs. Calculations coupling giant planet formation, within a modern version of the core accretion model, and subsequent evolution yield consistent determinations of the planet structure and evolution. Because of the uncertainties in the initial conditions, however, it is not possible to say whether young planets are faint or bright compared with low-mass young brown dwarfs. We review the effects of irradiation and evaporation on the evolution of short period planets and argue that substantial mass loss may have occurred for these objects. Concerning star formation, geometrical effects in protostar core collapse are examined by comparing 1D and 3D calculations. Spherical collapse is shown to overestimate the core inner density and temperature and thus to yield incorrect initial conditions for PMS or young brown dwarf evolution. Accretion is also shown to occur over a very limited fraction of the protostar surface. Accretion affects the evolution of young brown dwarfs and yields more compact structures for a given mass and age, thus fainter luminosities. This can lead to severe misinterpretations of the mass and/or age of young accreting objects from their location in the HR diagram. We argue that newborn stars and brown dwarfs should appear rapidly over an extended area in the HR diagram, depending on their accretion history, rather than on a well defined birth line. Finally, we suggest that the distinction between planets and brown dwarfs be based on an observational diagnostic, reflecting the different formation mechanisms between these two distinct populations, rather than on an arbitrary, confusing definition.Comment: Invited Review, Protostars and Planets V (Hawai, October 2005

Influenza virus morphogenesis and budding.

Influenza viruses are enveloped, negative stranded, segmented RNA viruses belonging to Orthomyxoviridae family. Each virion consists of three major sub-viral components, namely (i) a viral envelope decorated with three transmembrane proteins hemagglutinin (HA), neuraminidase (NA) and M2, (ii) an intermediate layer of matrix protein (M1), and (iii) an innermost helical viral ribonucleocapsid [vRNP] core formed by nucleoprotein (NP) and negative strand viral RNA (vRNA). Since complete virus particles are not found inside the cell, the processes of assembly, morphogenesis, budding and release of progeny virus particles at the plasma membrane of the infected cells are critically important for the production of infectious virions and pathogenesis of influenza viruses as well. Morphogenesis and budding require that all virus components must be brought to the budding site which is the apical plasma membrane in polarized epithelial cells whether in vitro cultured cells or in vivo infected animals. HA and NA forming the outer spikes on the viral envelope possess apical sorting signals and use exocytic pathways and lipid rafts for cell surface transport and apical sorting. NP also has apical determinant(s) and is probably transported to the apical budding site similarly via lipid rafts and/or through cortical actin microfilaments. M1 binds the NP and the exposed RNAs of vRNPs, as well as to the cytoplasmic tails (CT) and transmembrane (TM) domains of HA, NA and M2, and is likely brought to the budding site on the piggy-back of vRNP and transmembrane proteins. Budding processes involve bud initiation, bud growth and bud release. The presence of lipid rafts and assembly of viral components at the budding site can cause asymmetry of lipid bilayers and outward membrane bending leading to bud initiation and bud growth. Bud release requires fusion of the apposing viral and cellular membranes and scission of the virus buds from the infected cellular membrane. The processes involved in bud initiation, bud growth and bud scission/release require involvement both viral and host components and can affect bud closing and virus release in both positive and negative ways. Among the viral components, M1, M2 and NA play important roles in bud release and M1, M2 and NA mutations all affect the morphology of buds and released viruses. Disassembly of host cortical actin microfilaments at the pinching-off site appears to facilitate bud fission and release. Bud scission is energy dependent and only a small fraction of virus buds present on the cell surface is released. Discontinuity of M1 layer underneath the lipid bilayer, absence of outer membrane spikes, absence of lipid rafts in the lipid bilayer, as well as possible presence of M2 and disassembly of cortical actin microfilaments at the pinching-off site appear to facilitate bud fission and bud release. We provide our current understanding of these important processes leading to the production of infectious influenza virus particles

Phase-Dependent Properties of Extrasolar Planet Atmospheres

Recently the Spitzer Space Telescope observed the transiting extrasolar planets, TrES-1 and HD209458b. These observations have provided the first estimates of the day side thermal flux from two extrasolar planets orbiting Sun-like stars. In this paper, synthetic spectra from atmospheric models are compared to these observations. The day-night temperature difference is explored and phase-dependent flux densities are predicted for both planets. For HD209458b and TrES-1, models with significant day-to-night energy redistribution are required to reproduce the observations. However, the observational error bars are large and a range of models remains viable.Comment: 8 pages, 7 figures, accepted for publication in the Astrophysical Journa

Performance Analysis of Non-linear Jacketed CSTR Based on Different Control Strategies

This paper aims at finding the optimum controller for a jacketed Continuous Stirred Tank Reactor (CSTR) under non-ideal conditions. Various conventional control methods show poor response for non-linear processes. This paper outlines the design procedure of the Internal Model Controller (IMC) and Model Reference Adaptive Control (MRAC). The performance of the jacketed CSTR process is analyzed based on Internal Model Control and adaptive control. Simulation results have been compared with conventional PID contro

Sweet cherry cultivars influenced the growth and productivity under HDP

In a field experiment, to identify the best sweet cherry varieties for high density orcharding, maximum canopy volume (18.94 cm3) was recorded in variety ‘Steela’ and minimum in ‘Lambert’ while, ‘Bigarreau Napoleon’ had maximum TCSA (213 cm2). Trees grown under HDP have lower TCSA in comparison to normal density. Primary and secondary branch girth were maximum in ‘Bigarreau Napoleon’ whereas, annual extension growth and shoot thickness were high in ‘Steela’. Yield, yield efficiency and cumulative yield efficiency were registered maximum in ‘Bigarreau Napoleon’ and ‘Bigarreau Noir Grossa’ cultivars. Largest fruit weight, fruit length and fruit diameter were found maximum (10.16 g/fruit), (25.51 mm) (25.20 mm) respectively in ‘Bigarreau Napoleon’. Total soluble solids were found maximum in ‘Bigarreau Noir Grossa’ (17.30 0Brix) among the studied cultivars. Correlation matrix showed that TCSA had positive correlation with canopy volume, primary branch girth and secondary branch girth and fruit weight showed positive correlation with fruit length and fruit diameter

Landfill Leaching: an Experimental Investigation Using Column Apparatus

Source: ICHE Conference Archive - https://mdi-de.baw.de/icheArchiv