Destructive and Non-Destructive Testing of Bridge J857 Phelps County, Missouri : Strengthening and Testing to Failure of Bridge Decks

Concrete bridges are conventionally reinforced with steel bars and/or prestressed with steel tendons. When subjected to aggressive environments, corrosion of the reinforcing and prestressing steel occurs and eventually leads to premature structural deterioration and loss of serviceability. In addition, the increasing service loads as well as seismic upgrade requirements result in a need to strengthen many of these bridges. The use of externally bonded steel plates for flexural and shear strengthening of concrete members is well established. However, corrosion related problems have limited the use of this technique for outdoor application. Fiber reinforced polymer (FRP) composites are corrosion resistant and exhibit several properties that make them suitable for repair/strengthening of reinforced concrete (RC) structures. However, the database for performance of FRP strengthened RC members is based on small-scale specimens that do not account for the variation of boundary conditions of a real structure. Fullscale field tests can demonstrate the actual behavior of a structure and can lead to a better understanding of the performance of the system and therefore strengthening design requirements. This part of the research program aimed at demonstrating the feasibility and effectiveness of strengthening bridge RC decks with two systems of externally bonded FRP reinforcement to increase their flexural strengths as well as verify design methodology and capacity improvement. Two of the three simply supported decks were strengthened and tested to failure. One span was strengthened using near-surface mounted (NSM) CFRP rods while the second span was strengthened using externally bonded CFRP strips. The objective of the strengthening scheme was to increase the flexural capacity by approximately 30%. Each of the three spans was tested to failure by applying quasi-static load cycles. Test results indicate that the actual capacity of the bridge decks were higher than anticipated due to higher actual material strengths. In addition, the decks had end fixities that were estimated by comparison of experimental and theoretical results. The experimental moment capacities compared well with theoretical values based on the actual material properties obtained from laboratory testing and the determined end fixity. Strengthened decks exhibited ductile behavior prior to FRP failure. The short-term behavior of FRP strengthening system applications has been experimentally evaluated. Research into longterm performance should be conducted even though FRP used in highway bridges is expected to perform for a long time. The final report consists of three volumes. Volume I depicts the strengthening and testing to failure of the three bridge decks. Volume II focuses on the laboratory and field dynamic tests. Volume III focuses on the strengthening and testing to failure of the bridge piers

Fatigue Assessment of Traffic Signal Mast Arms based on Field Test Data under Natural Wind Gusts

In recent years, several states including Missouri, Wyoming, California, and Texas experienced fracture failures of traffic signal mast arms. Almost all the failures are associated with the propagation of defects or cracks. It is therefore imperative to evaluate existing mast arms using a simple yet accurate procedure. A statistical methodology is proposed to predict the fatigue life of signal mast arm structures on the basis of field-measured strain data. The annual occurrence of various stress levels is determined using the historical wind speed data in the vicinity of a mast arm structure and the strain readings of the structure under specific wind gusts. For each stress level, the crack initiation and propagation lives are estimated with the strain-life approach and the Paris crack-growth-rate model. They are combined to account for variable stresses by means of Miner\u27s rule and the root-mean-square model, respectively. The stress concentration factor around the arm-post connection is determined using a finite element model. The parameters in the life prediction models are determined with ASTM flat tension and compact tension tests. The proposed methodology was applied to a 12.8-m (42-ft) long octagonal mast arm and a 16.5-m (54-ft) long circular mast arm in Missouri. It is concluded that signal structures in perfect condition will not crack under natural wind gusts during their service life. However, the 16.5-m-long arm is likely to be vulnerable to tiny defects around the weld connection, but the 12.8-m-long arm is safe unless a visible crack exists

Evolution of a Complex Locus: Exon Gain, Loss and Divergence at the Gr39a Locus in Drosophila

Background. Gene families typically evolve by gene duplication followed by the adoption of new or altered gene functions. A different way to evolve new but related functions is alternative splicing of existing exons of a complex gene. The chemosensory gene families of animals are characterised by numerous loci of related function. Alternative splicing has only rarely been reported in chemosensory loci, for example in 5 out of around 120 loci in Drosophila melanogaster. The gustatory receptor gene Gr39a has four large exons that are alternatively spliced with three small conserved exons. Recently the genome sequences of eleven additional species of Drosophila have become available allowing us to examine variation in the structure of the Gr39a locus across a wide phylogenetic range of fly species. Methodology/Principal Findings. We describe a fifth exon and show that the locus has a complex evolutionary history with several duplications, pseudogenisations and losses of exons. PAML analyses suggested that the whole gene has a history of purifying selection, although this was less strong in exons which underwent duplication. Conclusions/Significance. Estimates of functional divergence between exons were similar in magnitude to functional divergence between duplicated genes, suggesting that exon divergence is broadly equivalent to gene duplication.Publisher PDFPeer reviewe

Accurate Determination of Phenotypic Information from Historic Thoroughbred Horses by Single Base Extension

Historic DNA have the potential to identify phenotypic information otherwise invisible in the historical, archaeological and palaeontological record. In order to determine whether a single nucleotide polymorphism typing protocol based on single based extension (SNaPshot™) could produce reliable phenotypic data from historic samples, we genotyped three coat colour markers for a sample of historic Thoroughbred horses for which both phenotypic and correct geotypic information were known from pedigree information in the General Stud Book. Experimental results were consistent with the pedigrees in all cases. Thus we demonstrate that historic DNA techniques can produce reliable phenotypic information from museum specimens.© 2010 Campana et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Utility of the new Movement Disorder Society clinical diagnostic criteria for Parkinson's disease applied retrospectively in a large cohort study of recent onset cases

Objective: To examine the utility of the new Movement Disorder Society (MDS) diagnostic criteria in a large cohort of Parkinson's disease (PD) patients. Methods: Recently diagnosed (<3.5 years) PD cases fulfilling United Kingdom (UK) brain bank criteria in Tracking Parkinson's, a UK multicenter prospective natural history study were assessed by retrospective application of the MDS criteria. Results: In 2000 cases, 1835 (91.7%) met MDS criteria for PD, either clinically established (n = 1261, 63.1%) or clinically probable (n = 574, 28.7%), leaving 165 (8.3%) not fulfilling criteria. Clinically established cases were significantly more likely to have limb rest tremor (89.3%), a good l-dopa response (79.5%), and olfactory loss (71.1%), than clinically probable cases (60.6%, 44.4%, and 34.5% respectively), but differences between probable PD and ‘not PD’ cases were less evident. In cases not fulfilling criteria, the mean MDS UPDRS3 score (25.1, SD 13.2) was significantly higher than in probable PD (22.3, SD 12.7, p = 0.016) but not established PD (22.9, SD 12.0, p = 0.066). The l-dopa equivalent daily dose of 341 mg (SD 261) in non-PD cases was significantly higher than in probable PD (250 mg, SD 214, p < 0.001) and established PD (308 mg, SD 199, p = 0.025). After 30 months' follow-up, 89.5% of clinically established cases at baseline remained as PD (established/probable), and 86.9% of those categorized as clinically probable at baseline remained as PD (established/probable). Cases not fulfilling PD criteria had more severe parkinsonism, in particular relating to postural instability, gait problems, and cognitive impairment. Conclusion: Over 90% of cases clinically diagnosed as early PD fulfilled the MDS criteria for PD. Those not fulfilling criteria may have an atypical parkinsonian disorder or secondary parkinsonism that is not correctly identified by the UK Brain Bank criteria, but possibly by the new criteria

Forensic Investigation of Failed Mast Arms of Traffic Signal Supported Structures

In Missouri, 11 traffic signal mast arms fractured at the arm-post weld connection in 7 years. To reduce this fatigue failure, the Missouri Department of Transportation developed a fatigue-resistant weld profile that increases the weld leg and reduces the slope of the weld at the toe. This study investigated causes of the failed arms, compared performance of new and old weld profiles, and suggested retrofitting measures for further investigation. The scope included a metallurgical investigation of one failed field mast arm, laboratory fatigue testing of five prototype mast arms (two new and three old profiles), and laboratory failure analysis of one arm tested to cracking. Metallographic and fractographic analyses indicated that the fatigue crack in the failed mast arm initiates near the weld toe of the arm due to undercutting, creating a sharp local toe angle. Location of undercutting at the heat-affected zone of the base material, where the material is softest, further contributed to early fatigue failure. Tests showed that the new weld profile does not consistently increase fatigue strength. Premature fracture surfaces of one tested arm indicated that the fatigue cracks initiate in an area at the weld toe as observed in the failed mast arm. Therefore, changing the weld profile alone is unlikely to increase mast arm fatigue life. Pinning the weldment surface at the weld toe of mast arms is suggested to increase the life of mast arms

Vascular disease and vascular risk factors in relation to motor features and cognition in early Parkinson's disease

Funded by Parkinson's UK National Institute for Health Research (NIHR) DeNDRoN network NIHR Newcastle Biomedical Research Unit Newcastle University NIHR funded Biomedical Research Centre in CambridgePeer reviewedPublisher PD

Selective lability of ruthenium(II) arene amino acid complexes.

A series of organometallic complexes of the form [(PhH)Ru(amino acid)](+) have been synthesized using amino acids able to act as tridentate ligands. The straightforward syntheses gave enantiomerically pure complexes with two stereogenic centers due to the enantiopurity of the chelating ligands. Complexes were characterized in the solid-state and/or solution-state where the stability of the complex allowed. The propensity toward labilization of the coordinatively saturated complexes was investigated. The links between complex stability and structural features are very subtle. Nonetheless, H/D exchange rates of coordinated amino groups reveal more significant differences in reactivity linked to metallocycle ring size resulting in decreasing stability of the metallocycle as the amino acid side-chain length increases. The behavior of these systems in acid is unusual, apparently labilizing the carboxylate residue of the amino acid. This acid-catalyzed hemilability in an organometallic is relevant to the use of Ru(II) arenes in medicinal contexts due to the relatively low pH of cancerous cells.TGS and MO thank the EPSRC for Studentships EP/P505445/1 and EP/K503/009/1, respectively.This is the final version of the article. It was first published by ACS at http://pubs.acs.org/doi/abs/10.1021/ic502051

Adhesion Molecule Expression in Polymorphic Light Eruption

Endothelial leukocyte adhesion molecule-1 (ELAM-1), vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1) are cytokine-regulated cell-surface Ieukocyte adhesion molecules. We have investigated the in vivo kinetics and pattern of expression of these adhesion molecules in relation to tissue accumulation of leukocytes in the photodermatosis, polymorphic light eruption (PMLE), which is characterized by dense perivascular leukocytic infiltration. Immunohistology was performed on biopsies taken at varying time points from PMLE lesions induced in 11 subjects by suberythemal solar simulated irradiation. Vascular endothelial ELAM-1 expression was first observed at 5 h, maximal at 24 to 72 h, and remained elevated at 6 d. VCAM-1, minimally expressed in control skin, was induced above background levels on endothelium and some perivascular cells after 24h and maintained at 6 d. Endothelial cell ICAM-1 expression was increased above control levels at 72h and 6 d. Keratinocyte ICAM-1 expression, most marked overlying areas of dermal leukocytic infiltration, began at 5h and was strong at 72h and 6 d. In addition to lymphocytes, significant number of neutrophils of but not eosinophils were detected in the dermal leukocytic infiltrate that appeared at 5h and persisted at 6 d. The pattern of adhesion molecule expression that we have observed is similar to that seen in normal skin during a delayed hypersensitivity reaction: These observations support an immunologic basis for PMLE