Transcriptional profiling of olfactory system development identifies distal antenna as a regulator of subset of neuronal fates

Drosophila uses 50 different olfactory receptor neuron (ORN) classes that are clustered within distinct sensilla subtypes to decipher their chemical environment. Each sensilla subtype houses 1–4 ORN identities that arise through asymmetric divisions of a single sensory organ precursor (SOP). Despite a number of mutational studies investigating the regulation of ORN development, a majority of the transcriptional programs that lead to the different ORN classes in the developing olfactory system are unknown. Here we use transcriptional profiling across the time series of antennal development to identify novel transcriptional programs governing the differentiation of ORNs. We surveyed four critical developmental stages of the olfactory system: 3rd instar larval (prepatterning), 8 hours after puparium formation (APF, SOP selection), 40 hrs APF (neurogenesis), and adult antennae. We focused on the expression profiles of olfactory receptor genes and transcription factors—the two main classes of genes that regulate the sensory identity of ORNs. We identify distinct clusters of genes that have overlapping temporal expression profiles suggesting they have a key role during olfactory system development. We show that the expression of the transcription factor distal antenna (dan) is highly similar to other prepatterning factors and is required for the expression of a subset of ORs

A Functionally Conserved Gene Regulatory Network Module Governing Olfactory Neuron Diversity

Sensory neuron diversity is required for organisms to decipher complex environmental cues. In Drosophila, the olfactory environment is detected by 50 different olfactory receptor neuron (ORN) classes that are clustered in combinations within distinct sensilla subtypes. Each sensilla subtype houses stereotypically clustered 1–4 ORN identities that arise through asymmetric divisions from a single multipotent sensory organ precursor (SOP). How each class of SOPs acquires a unique differentiation potential that accounts for ORN diversity is unknown. Previously, we reported a critical component of SOP diversification program, Rotund (Rn), increases ORN diversity by generating novel developmental trajectories from existing precursors within each independent sensilla type lineages. Here, we show that Rn, along with BarH1/H2 (Bar), Bric-à-brac (Bab), Apterous (Ap) and Dachshund (Dac), constitutes a transcription factor (TF) network that patterns the developing olfactory tissue. This network was previously shown to pattern the segmentation of the leg, which suggests that this network is functionally conserved. In antennal imaginal discs, precursors with diverse ORN differentiation potentials are selected from concentric rings defined by unique combinations of these TFs along the proximodistal axis of the developing antennal disc. The combinatorial code that demarcates each precursor field is set up by cross-regulatory interactions among different factors within the network. Modifications of this network lead to predictable changes in the diversity of sensilla subtypes and ORN pools. In light of our data, we propose a molecular map that defines each unique SOP fate. Our results highlight the importance of the early prepatterning gene regulatory network as a modulator of SOP and terminally differentiated ORN diversity. Finally, our model illustrates how conserved developmental strategies are used to generate neuronal diversity

Coherent Bayesian inference on compact binary inspirals using a network of interferometric gravitational wave detectors

Presented in this paper is a Markov chain Monte Carlo (MCMC) routine for conducting coherent parameter estimation for interferometric gravitational wave observations of an inspiral of binary compact objects using data from multiple detectors. The MCMC technique uses data from several interferometers and infers all nine of the parameters (ignoring spin) associated with the binary system, including the distance to the source, the masses, and the location on the sky. The Metropolis-algorithm utilises advanced MCMC techniques, such as importance resampling and parallel tempering. The data is compared with time-domain inspiral templates that are 2.5 post-Newtonian (PN) in phase and 2.0 PN in amplitude. Our routine could be implemented as part of an inspiral detection pipeline for a world wide network of detectors. Examples are given for simulated signals and data as seen by the LIGO and Virgo detectors operating at their design sensitivity.Comment: 10 pages, 4 figure

Patterns of transcriptional parallelism and variation in the developing olfactory system of Drosophila species

Organisms have evolved strikingly parallel phenotypes in response to similar selection pressures suggesting that there may be shared constraints limiting the possible evolutionary trajectories. For example, the behavioral adaptation of specialist Drosophila species to specific host plants can exhibit parallel changes in their adult olfactory neuroanatomy. We investigated the genetic basis of these parallel changes by comparing gene expression during the development of the olfactory system of two specialist Drosophila species to that of four other generalist species. Our results suggest that the parallelism observed in the adult olfactory neuroanatomy of ecological specialists extends more broadly to their developmental antennal expression profiles, and to the transcription factor combinations specifying olfactory receptor neuron (ORN) fates. Additionally, comparing general patterns of variation for the antennal transcriptional profiles in the adult and developing olfactory system of the six species suggest the possibility that specific, non-random components of the developmental programs underlying the Drosophila olfactory system harbor a disproportionate amount of interspecies variation. Further examination of these developmental components may be able to inform a deeper understanding of how traits evolve

Short GRB and binary black hole standard sirens as a probe of dark energy

Observations of the gravitational radiation from well-localized, inspiraling compact object binaries can measure absolute source distances with high accuracy. When coupled with an independent determination of redshift through an electromagnetic counterpart, these standard sirens can provide an excellent probe of the expansion history of the Universe and the dark energy. Short gamma-ray bursts, if produced by merging neutron star binaries, would be standard sirens with known redshifts detectable by ground-based GW networks such as LIGO-II, Virgo, and AIGO. Depending upon the collimation of these GRBs, a single year of observation of their gravitational waves can measure the Hubble constant to about 2%. When combined with measurement of the absolute distance to the last scattering surface of the cosmic microwave background, this determines the dark energy equation of state parameter w to 9%. Similarly, supermassive binary black hole inspirals will be standard sirens detectable by LISA. Depending upon the precise redshift distribution, 100 sources could measure w at the 4% level.Comment: 8 pages, submitted to PR

Measurement of the B-Meson Inclusive Semileptonic Branching Fraction and Electron-Energy Moments

We report a new measurement of the B-meson semileptonic decay momentum spectrum that has been made with a sample of 9.4/fb of electron-positron annihilation data collected with the CLEO II detector at the Y(4S) resonance. Electrons from primary semileptonic decays and secondary charm decays were separated by using charge and angular correlations in Y(4S) events with a high-momentum lepton and an additional electron. We determined the semileptonic branching fraction to be (10.91 +- 0.09 +- 0.24)% from the normalization of the electron-energy spectrum. We also measured the moments of the electron energy spectrum with minimum energies from 0.6 GeV to 1.5 GeV.Comment: 36 pages postscript, als available through http://w4.lns.cornell.edu/public/CLNS/, Submitted to PRD (back-to-back with preceding preprint hep-ex/0403052

Detector Description and Performance for the First Coincidence Observations between LIGO and GEO

For 17 days in August and September 2002, the LIGO and GEO interferometer gravitational wave detectors were operated in coincidence to produce their first data for scientific analysis. Although the detectors were still far from their design sensitivity levels, the data can be used to place better upper limits on the flux of gravitational waves incident on the earth than previous direct measurements. This paper describes the instruments and the data in some detail, as a companion to analysis papers based on the first data.Comment: 41 pages, 9 figures 17 Sept 03: author list amended, minor editorial change

Setting upper limits on the strength of periodic gravitational waves from PSR J1939+2134 using the first science data from the GEO 600 and LIGO detectors

Data collected by the GEO 600 and LIGO interferometric gravitational wave detectors during their first observational science run were searched for continuous gravitational waves from the pulsar J1939+2134 at twice its rotation frequency. Two independent analysis methods were used and are demonstrated in this paper: a frequency domain method and a time domain method. Both achieve consistent null results, placing new upper limits on the strength of the pulsar's gravitational wave emission. A model emission mechanism is used to interpret the limits as a constraint on the pulsar's equatorial ellipticity

Analysis of LIGO data for gravitational waves from binary neutron stars

We report on a search for gravitational waves from coalescing compact binary systems in the Milky Way and the Magellanic Clouds. The analysis uses data taken by two of the three LIGO interferometers during the first LIGO science run and illustrates a method of setting upper limits on inspiral event rates using interferometer data. The analysis pipeline is described with particular attention to data selection and coincidence between the two interferometers. We establish an observational upper limit of $\mathcal{R}<$1.7 \times 10^{2}$per year per Milky Way Equivalent Galaxy (MWEG), with 90coalescence rate of binary systems in which each component has a mass in the range 1--3$M_\odot$.Comment: 17 pages, 9 figure