Smoothelin-like 2 Inhibits Coronin-1B to Stabilize the Apical Actin Cortex during Epithelial Morphogenesis

The actin cortex is involved in many biological processes and needs to be significantly remodeled during cell differentiation. Developing epithelial cells construct a dense apical actin cortex to carry out their barrier and exchange functions. The apical cortex assembles in response to three-dimensional (3D) extracellular cues, but the regulation of this process during epithelial morphogenesis remains unknown. Here, we describe Smoothelin-like 2 (SMTNL2) function, a member of the smooth-muscle related Smoothelin protein family, in apical cortex maturation. SMTNL2 is induced during the development of multiple epithelial tissues and localizes to the apical and junctional actin cortex in intestinal and kidney epithelial cells. SMTNL2 deficiency leads to membrane herniations in the apical domain of epithelial cells, indicative of cortex abnormalities. We find that SMTNL2 binds to actin filaments and is required to slow down the turnover of apical actin. We also characterize the SMTNL2 proximal interactome and find that SMTNL2 executes its functions partly through inhibition of Coronin-1B. While Coronin-1B-mediated actin dynamics are required for early morphogenesis, its sustained activity is detrimental for the mature apical shape. SMTNL2 binds to Coronin-1B through its N-terminal coiled-coil region and negates its function to stabilize the apical cortex. In sum, our results unveil a mechanism for regulating actin dynamics during epithelial morphogenesis, providing critical insights on the developmental control of the cellular corte

HTLV-1 infection in solid organ transplant donors and recipients in Spain

HTLV-1 infection is a neglected disease, despite infecting 10-15 million people worldwide and severe illnesses develop in 10% of carriers lifelong. Acknowledging a greater risk for developing HTLV-1 associated illnesses due to immunosuppression, screening is being widely considered in the transplantation setting. Herein, we report the experience with universal HTLV testing of donors and recipients of solid organ transplants in a survey conducted in Spain. All hospitals belonging to the Spanish HTLV network were invited to participate in the study. Briefly, HTLV antibody screening was performed retrospectively in all specimens collected from solid organ donors and recipients attended since the year 2008. A total of 5751 individuals were tested for HTLV antibodies at 8 sites. Donors represented 2312 (42.2%), of whom 17 (0.3%) were living kidney donors. The remaining 3439 (59.8%) were recipients. Spaniards represented nearly 80%. Overall, 9 individuals (0.16%) were initially reactive for HTLV antibodies. Six were donors and 3 were recipients. Using confirmatory tests, HTLV-1 could be confirmed in only two donors, one Spaniard and another from Colombia. Both kidneys of the Spaniard were inadvertently transplanted. Subacute myelopathy developed within 1 year in one recipient. The second recipient seroconverted for HTLV-1 but the kidney had to be removed soon due to rejection. Immunosuppression was stopped and 3 years later the patient remains in dialysis but otherwise asymptomatic. The rate of HTLV-1 is low but not negligible in donors/recipients of solid organ transplants in Spain. Universal HTLV screening should be recommended in all donor and recipients of solid organ transplantation in Spain. Evidence is overwhelming for very high virus transmission and increased risk along with the rapid development of subacute myelopathy

Anales de Edafología y Agrobiología Tomo 32 Número 9-10

Aportaciones para un mejor conocimiento de los suelos desarrollados sobre materiales calizos consolidados, por J. L. Moreno Alvarez y T. Badorrey Peracho.-- Estudio biológico y químico de Erica umbellata L., por M. Consolación Salas, Antonio Ballester y Ernesto Vieitez.--Contenido en metales de la nitrito reductasa del alga Chlorella, por J. Cárdenas, F. D. Pineda, F. F. de la Rosa, J. L. Barea y J. Rivas.-- Andosuelos de la provincia de Gerona. I. Estudio de sustancias minerales amorfas, por J. Rodríguez Sanchidrián y F Monturiol Rodríguez.-- Minerales de la arcilla en suelos de la provincia de Granada, por J. L. Martín Vivaldi, E. Galán Huertos y F. López Aguayo.-- Influence of foliar application of macro-nutrient on the plant growth and rnultiplication of viruses. I. Foliar application of nitrogen nutrient to tomato and its effect on host growth and potato virus X multiplication, by Rajendra Singh and Tej Pratap Mall.-- Heterodera Schachtii Schmidt, 1871 (Nematoda: Heteroderidae) en los suelos de las Islas Canarias, por A. Bello y Mª. D. Romero.-- Utiliización de una nueva fórmula de cálculo para la evaporación de Piché en regiones húmedas de España, por Jesús Seco, Angela Calvo y José Garmendía.-- Contribución al estudio de la técnica de Walkley y Black para la determinación de carbono orgánico de suelos, por Luisa Prat Pérez y Benito Sánchez.-- Estudio de la dinámica del crecimiento del limón en el sureste español, por O. Carpena, F. Romojaro, C. Alearaz y S. Llorente.-- Efecto de la aplicación de acetileno y ácido 2-cloroetilfosfónico sobre la fructificación y cosecha del olivo, por A. J. Sánchez-Raya, J. P. Donaire y L. Recalde.—Notas científicas.-- Estudio de fosfatasa ácida en plantas de A tropa belladona L. recogidas en otoño, por M. R. Felipe Antón y N. Velázquez Sánchez.—Notas.-- Nombramiento de Presidente del C. S. I. C.-- Constitución de la nueva Junta de Gobierno del Patronato Alonso de Herrera.-- Nombramiento de Consejeros de Número.-- Nombramiento de Consejeros Adjuntos.-- Designación del Comité Nacional de la Unión Internacional de Investigaciones sobre el Cuaternario.-- Rector de la Universidad de La Laguna.-- Distinción al Pro f. Zorita.-- European Grassland Federation (5th General Meeting).-- Reuniones nacionales.-- 1ª Reunión Nacional del Grupo de Trabajo del Cuaternario.-- Laboratorio de Micromorfología de Suelos.-- Próximo Congreso de Micromorfología.-- Reunión en La Mayora.-- BibliografíaPeer reviewed2019-08.- CopyBook.- Libnova.- Biblioteca ICA

A comprehensive fluorescent sensor for spatiotemporal cell cycle analysis in Arabidopsis

Assessing cell proliferation dynamics is crucial to understand the spatiotemporal control of organogenesis. Here we have generated a versatile fluorescent sensor, PlaCCI (plant cell cycle indicator) on the basis of the expression of CDT1a-CFP, H3.1-mCherry and CYCB1;1-YFP, that identifies cell cycle phases in Arabidopsis thaliana. This tool works in a variety of organs, and all markers and the antibiotic resistance are expressed from a single cassette, facilitating the selection in mutant backgrounds. We also show the robustness of PlaCCI line in live-imaging experiments to follow and quantify cell cycle phase progressionEuropean Union, and by institutional grants from Banco de Santander and Fundación Ramon Areces to the Centro de Biología Molecular Severo Ochoa.Peer reviewe

Sfrp3 modulates stromal–epithelial crosstalk during mammary gland development by regulating Wnt levels

Mammary stroma is essential for epithelial morphogenesis and development. Indeed, postnatal mammary gland (MG) development is controlled locally by the repetitive and bi-directional cross-talk between the epithelial and the stromal compartment. However, the signalling pathways involved in stromal–epithelial communication are not entirely understood. Here, we identify Sfrp3 as a mediator of the stromal–epithelial communication that is required for normal mouse MG development. Using Drosophila wing imaginal disc, we demonstrate that Sfrp3 functions as an extracellular transporter of Wnts that facilitates their diffusion, and thus, their levels in the boundaries of different compartments. Indeed, loss of Sfrp3 in mice leads to an increase of ductal invasion and branching mirroring an early pregnancy state. Finally, we observe that loss of Sfrp3 predisposes for invasive breast cancer. Altogether, our study shows that Sfrp3 controls MG morphogenesis by modulating the stromal-epithelial cross-talk during pubertal development.MINECO (BFU2015-71244-ERC; BFU2014-52125-REDT; BFU2014-57831), and Fundacion Ramón Areces to F.M-B. M.D.B. was supported by a MINECOFPI 2015 PhD fellowship. M.H. was supported by a MINECO-FPI 2012 PhD fellowship. M.B.F. was supported by a La-Caix

Mini-Multi Interface Box Simulator (MMIBS)

Article number 012100 Code 166960Today’s military and commercial aircraft avionics and general systems are built around complex networks of intelligent devices. Maintenance and troubleshooting of these systems often require access to the data bus or networks connecting the system components. Whether it is on the integration test bench, Final Assembly Line (FAL) or at the Maintenance and Repair Organization (MRO), these tasks are usually performed by means of many, expensive, heavy and intrusive equipment. The MMIBS (Mini-Multi Interface Box Simulator) is a very compact, portable, multipurpose, lightweight, low-power, and easy-of-use electronics test means intended to improve digital aircraft ground testing and maintenance troubleshooting. It is capable of perform functional testing and simulating aeronautical equipment through most common aeronautical communication interfaces, such as AFDX, CAN BUS, ARINC 429, MIL-STD-1553, RS-422, RS-232, RS-485, Discrete and Analog I/O and RVDT (Rotary Variable Differential Transformer) reading capabilities. Besides, the system is highly configurable and reprogrammable by means of intuitive Python-based scripting. System is also able to be controlled with an intuitive, user-friendly and dedicated HMI (Human Machine Interface). In this mode, the user can conform a MMIBS WIFI network, allowing multiple MMIBS operating in parallel to cover complex testing scenarios. The MMIBS device integrates all the aeronautical communication and signaling in a volume of 12x12x12cm and weight of 1200g and provides an autonomy of 5 hours. In this way, our solution combines performance, reliability, low maintenance costs, and ease-of-use to support efficient ground maintenance operations

Anales de Edafología y Agrobiología Tomo 33 Número 11-12

Efecto de la etiolación en la rizogénesis de Castanea sativa Mili., por A. M. Vieitez y E. Vieitez.-- Efecto de los déficits hídricos en el contenido en hidratos de carbono y en la producción de tubérculos de patata (Solanum tuberosum L.), por M. Sánchez-Díaz, J. Salaverri y J. Grau.--Inhibición de la síntesis de caoiinita vor diversos extractos acuosos de restos vegetales, por F. Guitián Ojea y V. Coladas Calvo.-- Gibsita en suelos sobre granitos del sistema central, por M. Sánchez Camazano.-- El nematodo de los cítricos, Tylenchulus semipenetrans Cobb., en España, por M. P. Alvira.-- Estudio de la migración de arcilla en Jos suelos «Praderas arenosas gris-amarillentas desarrollados sobre las areniscas de Tacuarembo (Uruguay), por A. van Wambeke y D. Barrera Saquieres.-- Crecimiento y materia seca en maíz (Zea mays L.), por Benito Sánchez y Gerardo.-- Regulación de la NADH-nitrato reductasa de Chlamydomonas reinhardi: inactivación reversible in vivo e in vitro, por J. L. Barea, F. M. Sosa y T. Ortega.-- Andosuelos desarrollados sobre íihtas en Ulzama (Navarra), por J. Iñiguez y E. Barragán.-- Mineralogía de las terrazas del río Duero desde Gormaz hasta Peñafiel, por M. A. Hoyos Guerrero, S. Leguey y J. Rodríguez Martínez.-- Estudio de suelos desarrollados sobre rocas cristalinas. I. Zona Ojén-Mijas, por J. Fernándes, J. lñgues y J. Aguilar.-- Estudio de suelos desarrollados sobre rocas cristalinas. II. Zona de Antequera, por J. Fernándes, J. lñiguez y J. Aguilar.—Notas.-- Fallecimiento del Consejero de Número del Patronato Excmo. Sr. D. Miguel Odriozola Pietá.-- El Prof. Primo Yúfera, nuevo Presidente del C. S. I. C.-- Nombramiento de Consejeros de Número.-- Nombramiento de Presidente y Secretario de la División de Ciencias.-- Cese del Presidente del Patronato Alonso de Herrera.-- Propuesta de nuevo Presidente del Patronato Alonso de Herrera.-- Cambio de nombre del Instituto de Investigaciones Geológicas, Edafológicas y Agrobiológicas de Galicia.-- Nombramiento de representante del Patronato Alonso de Herrera» en el Consejo de Dirección del I.N.I. A.-- Cambio en la dirección del Jardín Botánico.-- Nombramiento de Vocales del Crida de la Coruña.-- Nombramiento de Vicedirector del Instituto de Alimentación y Productividad Animal .-- Vocales del Consejo Técnico Administrativo de la Estación Experimental La Mayora.-- Becas ofrecidas por el I. N. I. A.-- Nuevo Prof. Agregado de la Universidad Autónoma de Madrid.-- Autorizaciones para impartir docencia.-- Congresos, reuniones y desplazamientos al extranjero y nacionales.-- Reunión del Grupo de Trabajo de Normalización de Métodos Analíticos.-- La micromorfología del suelo en Polonia.-- Real Academia de Farmacia.-- Instituto Palinológico de León.-- BibliografíaPeer reviewe