Expression signatures of cisplatin- and trametinib-treated early-stage medaka melanomas

Small aquarium fish models provide useful systems not only for a better understanding of the molecular basis of many human diseases, but also for first-line screening to identify new drug candidates. For testing new chemical substances, current strategies mostly rely on easy to perform and efficient embryonic screens. Cancer, however, is a disease that develops mainly during juvenile and adult stage. Long-term treatment and the challenge to monitor changes in tumor phenotype make testing of large chemical libraries in juvenile and adult animals cost prohibitive. We hypothesized that changes in the gene expression profile should occur early during anti-tumor treatment, and the disease-associated transcriptional change should provide a reliable readout that can be utilized to evaluate drug-induced effects. For the current study, we used a previously established medaka melanoma model. As proof of principle, we showed that exposure of melanoma developing fish to the drugs cisplatin or trametinib, known cancer therapies, for a period of seven days is sufficient to detect treatment-induced changes in gene expression. By examining whole body transcriptome responses we provide a novel route toward gene panels that recapitulate anti-tumor outcomes thus allowing a screening of thousands of drugs using a whole-body vertebrate model. Our results suggest that using disease-associated transcriptional change to screen therapeutic molecules in small fish model is viable and may be applied to pre-clinical research and development stages in new drug discovery

Premarital sex in Vietnam: Is the current concern with adolescent reproductive health warranted?

To the extent that research on Vietnamese adolescents has been conducted, it has been concerned with unprotected and unsanctioned sexual activity and its health consequences, namely abortion and sexually transmitted diseases, especially HIV. The question we pose is whether this concern is warranted. Is the population community justified in focusing its attention on early sexual activity and HIV risk? Even if the sexual behavior of young people can be considered problematic, are there perhaps other aspects of young people’s lives that should give us greater pause? The paper reviews the literature on adolescent sexual behavior in Vietnam and analyzes data on premarital sex and reproductive behavior from a 1999 survey conducted in six provinces among nearly 1,500 adolescent boys and girls aged 15-22. Data on other aspects of young people’s lives are summarized, in particular schooling and work, in order to put the sexual activity data in perspective. We conclude that the lack of adequate employment opportunities may be more of a threat to adolescent reproductive health than risky sexual behaviors per se—a situation that effective economic policies can remedy

The changing social environment for adolescents in Kassena-Nankana District of northern Ghana: Implications for reproductive behavior

This study reports on a primarily qualitative investigation of adolescent reproductive behavior in the Kassena-Nankana District, a rural isolated area in northern Ghana, where traditional patterns of marriage, family formation, and social organization persist. The study is based on in-depth interviews and focus groups with adolescents, parents, chiefs and traditional leaders, teachers, youth leaders, and health workers, supplemented by quantitative data from the 1996 wave of a panel survey of reproductive-aged women conducted by the Navrongo Health Research Centre. The social environment faced by adolescent boys and girls in the Kassena-Nankana District and its links to reproductive behavior are described. The principal question is whether even in this remote, rural area, the environment has been altered in ways that have undermined traditional sexual and reproductive patterns. The survey data indicate a considerable increase in educational attainment among younger women. In addition, it appears that the incidence of early marriage has begun to decline. The qualitative data suggest that social institutions, systems, and practices such as female circumcision, which previously structured the lives of adolescent boys and girls, have eroded, leading to an apparent increase in premarital sexual activity. The implications of these developments for adolescent health and well-being in the District are discussed

Premarital sex and school dropout in Kenya: Can schools make a difference?

Although an overall decline has occurred in adolescent fertility in Kenya, the proportion of births to teenagers that takes place prior to marriage is rising. At the same time that premarital sex and childbearing have increased, educational participation has expanded considerably, especially for girls. Using data from nearly 600 adolescents aged 12-19 in combination with data collected from 33 primary schools that the adolescents attended, this paper explores whether certain aspects of the school environment affect the likelihood of early and unprotected sex among adolescent girls and boys in three districts of Kenya. Because of the concern with “schoolgirl pregnancy” in Kenya, the paper also explores the temporal relationship between premarital sex and pregnancy, and school dropout. The results suggest that, although neither the school nor the home influences whether boys engage in premarital sex, for girls, a school characterized by girl-friendly teachers and a gender-neutral atmosphere, and a home containing female role models and the extra support that two parents can provide, reduce the risk of premarital sex. On the other hand, girls are more likely to engage in premarital sex if they attend schools where considerable pressure to have sex is reported. The school environment also appears to have an impact on whether or not sexually active boys choose to use contraceptives. A gender-neutral environment leads to greater contraceptive use among boys, as do schools where students have greater knowledge of reproduction. Finally, even if certain school characteristics significantly affect the risk of premarital sex for girls, the data indicate that pregnancy is not the primary reason that girls leave school early

Rapid Circumstellar Disk Evolution and an Accelerating Star Formation Rate in the Infrared Dark Cloud M17 SWex

We present a catalog of 840 X-ray sources and first results from a 100 ks Chandra X-ray Observatory imaging study of the filamentary infrared dark cloud G014.225$-$00.506, which forms the central regions of a larger cloud complex known as the M17 southwest extension (M17 SWex). In addition to the rich population of protostars and young stellar objects with dusty circumstellar disks revealed by Spitzer Space Telescope archival data, we discover a population of X-ray-emitting, intermediate-mass pre--main-sequence stars (IMPS) that lack infrared excess emission from circumstellar disks. We model the infrared spectral energy distributions of this source population to measure its mass function and place new constraints on the inner dust disk destruction timescales for 2-8 $M_{\odot}$ stars. We also place a lower limit on the star formation rate (SFR) and find that it is quite high ($\dot{M}\ge 0.007~M_{\odot}$ yr$^{-1}$), equivalent to several Orion Nebula Clusters in G14.225$-$0.506 alone, and likely accelerating. The cloud complex has not produced a population of massive, O-type stars commensurate with its SFR. This absence of very massive (${\ge}20~M_{\odot}$) stars suggests that either (1) M17 SWex is an example of a distributed mode of star formation that will produce a large OB association dominated by intermediate-mass stars but relatively few massive clusters, or (2) the massive cores are still in the process of accreting sufficient mass to form massive clusters hosting O stars.Comment: 29 pages, 9 figures, accepted to Ap

Postharvest dehydration of Syrah grapes (Vitis vinifera L.) under controlled temperature conditions with real-time monitoring of mass loss

The partial dehydration of grapes after harvest aimed at winemaking has been shown to be a process that brings increased concentration of sugar and phenolic compounds in the must, which affects the quality of the wines produced. However, the works developed so far have studied the process for temperatures up to a maximum of 25°C and air velocity lower than 1 m.s-1. This study aimed to analyze the physical-chemical changes concentration of total soluble solids (TSS) and phenolic compounds (PC) after partial dehydration of 'Syrah' grapes subjected to two treatments combining two temperatures and one air velocity (T1= 22.9°C/1.79 m.s-1 and T2 = 37.1°C/1.79 m.s-1) and relative humidity of 40%. The water loss of the grapes was approximately 14% and the drying process lasted between 34 and 68 h for treatments T2 and T1, respectively. We experimentally and statistically verified that the treatments promoted significant increase in TSS and PC; however, for PC at the temperature of 37.1°C, the increase accounted for approximately 27%, whereas, for the temperature of 22.9°C, it was only 12%. For TSS, the increase was on average 12.47±0.9% between both treatments. The results demonstrate that it is possible to moderately dry grapes, which consequently results in improvements in their chemical composition and can improve the quality of wine.104229234COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPSem informação2012/20236-6; 2010/15663-

Partial dehydration of 'Niagara Rosada' GRAPES (Vitis labrusca L.) targeting increased concentration of phenolic compounds and soluble solids

The partial dehydration of grapes after harvest and aimed wine-making, has been shown to be a process that brings increased concentration of sugar and phenolic compounds in the must, which results in the quality of the wines produced. However, the works developed so far studied the process for temperatures up to a maximum of 25°C and air velocity less than 1 m.s-1. This study aimed to analyze the physical and chemical changes (concentration of total soluble solids (TSS) and phenolic compounds (CPC)) after partial dehydration of 'Niagara Rosada' grapes at the temperature subjected to two treatments combining two temperatures and one air velocity (T1= 22.9°C/1.79 m.s-1 and T2 = 37.1°C/1.79 m.s-1), and relative humidity of approximately 40%. The loss of water in the grapes was approximately 14% and the drying process lasted between 20 to 50 h for the treatments T1 and T2, respectively. We experimentally and statistically verified that the treatments promoted significant increase in TSS and CPC; however, for CPC at the temperature of 37.1°C, the increase accounted for approximately 29%, whereas, for the temperature of 22.9°C, it was only 5%. For TSS, the increase was on average 14.4 ± 3.9% between both treatments.Key words: Chaptalization, temperature, air velocity, winemaking

Weed identification in sugarcane plantation through images taken from remotely piloted aircraft (RPA) and kNN classifier

The sugarcane is one of the most important crops in Brazil, the world´s largest sugar producer and the second largest ethanol producer. The presence of weeds in the sugarcane plantation can cause losses up to 90% of the production, caused by the competition for light, water and nutrients, between the crop and the weeds. Usually sugarcane plantations occupy large fields, and due to this, the weeds control is mostly chemical, which is more practical and cheaper than mechanical control. In the chemical control, the dosage and type of herbicides has been calculated by sampling, which causes problems of waste and misapplication of herbicides, since the degree of infestation may be variant from one location to another, as well as the species presents in the plantation. In order to avoid unnecessary waste in the herbicides application, there are some studies about weed identification using images taken from satellites, solution that have proved to have the advantage of covering the whole plantation, solving the problems of sample surveying, nevertheless, this method its dependent of a high weed density to ensure a good pattern recognition and its affected by the influence of clouds in the imagery quality. This work proposes a system for weed identification based on pattern recognition in imagery taken from a Remotely Piloted Aircraft (RPA). The RPA is able to fly at low altitude, so it is possible to take images closer to the plants and make the weed identification even in low infestation levels. In an initial evaluation, the system reached an overall accuracy of 83.1% and kappa coefficient of 0.775, using k-Nearest Neighbors (kNN) classifier.5621121

The decline of female circumcision in Egypt: Evidence and interpretation

Female circumcision is widespread in Egypt. Research suggests that the practice persists because of a belief that circumcision will moderate female sexuality, that it will assure a girl’s marriageability, and that it is sanctioned by Islam. Using data from a nationally representative survey of adolescents, this paper investigates the prevalence and social correlates of circumcision among girls aged 10-19, the circumstances surrounding the procedure, and the attitudes of adolescents towards it. While the vast majority of adolescents are circumcised, a life table analysis indicates that girls today are at least 10 percentage points less likely to undergo female circumcision than were their mothers. Circumcision may have begun to decline prior to the time when the current cohort of girls were at risk; however, the data hint at a temporal association between the decline and the 1994 International Conference on Population and Development (ICPD) in Cairo, a time when the campaign against circumcision gained momentum. Over half of circumcised girls reported that the procedure was performed by a physician or nurse rather than a traditional practitioner. This represents a substantial increase over rates of “medicalized” circumcision found among earlier cohorts of Egyptian women. Even among circumcised girls, support for the practice is by no means universal, with 14 percent saying they think the procedure is unnecessary and a further 28 percent expressing ambivalence. A multivariate analysis indicates that girls who have been or are currently in school, who live in urban governorates, and who are older are more likely to believe that circumcision is not obligatory. When the analysis includes boys as well as circumcised girls, a large gender gap emerges, with boys considerably more supportive of the practice than are their female counterparts

The decline of female circumcision in Egypt: Evidence and interpretation [Arabic]

Female circumcision is widespread in Egypt. Research suggests that the practice persists because of a belief that circumcision will moderate female sexuality, that it will assure a girl’s marriageability, and that it is sanctioned by Islam. Using data from a nationally representative survey of adolescents, this paper investigates the prevalence and social correlates of circumcision among girls aged 10-19, the circumstances surrounding the procedure, and the attitudes of adolescents towards it. While the vast majority of adolescents are circumcised, a life table analysis indicates that girls today are at least 10 percentage points less likely to undergo female circumcision than were their mothers. Circumcision may have begun to decline prior to the time when the current cohort of girls were at risk; however, the data hint at a temporal association between the decline and the 1994 International Conference on Population and Development (ICPD) in Cairo, a time when the campaign against circumcision gained momentum. Over half of circumcised girls reported that the procedure was performed by a physician or nurse rather than a traditional practitioner. This represents a substantial increase over rates of “medicalized” circumcision found among earlier cohorts of Egyptian women. Even among circumcised girls, support for the practice is by no means universal, with 14 percent saying they think the procedure is unnecessary and a further 28 percent expressing ambivalence. A multivariate analysis indicates that girls who have been or are currently in school, who live in urban governorates, and who are older are more likely to believe that circumcision is not obligatory. When the analysis includes boys as well as circumcised girls, a large gender gap emerges, with boys considerably more supportive of the practice than are their female counterparts