Dipicolinic Acid Release and the Germination of Alicyclobacillus acidoterrestris Spores under Nutrient Germinants

The presence of Alicyclobacillus, a thermoacidophilic and spore-forming bacterium, in acidic fruit juices poses a serious problem for the processing industry. A typical sign of spoilage in contaminated juices is a characteristic phenolic off-flavour associated with the produc­tion of guaiacol. Spores are formed in response to starvation and in a natural environment re-access the nutrients, e.g.: L-alanine and AGFK – a mixture of asparagine, glucose, fructose and potassium, triggers germination. The aim of this study was to estimate the impact of L-alanine and AGFK on the germination of the spores of two Alicyclobacillus acidoterrestris strains and to evaluate the relationship of the germination rate with dipicolinic acid (DPA) release. The spores were suspended in apple juice or in buffers at pH 4 and pH 7, followed by the addition of L-alanine and AGFK. Suspensions were or were not subjected, to a temperature of 80°C/10 min and incubated for various periods of time at 45°C. Optical density (OD660) was used to estimate the number of germinated spores. The amount of DPA released was determined using HPLC. The results indicate that the degree of germination of A. acidoterrestris spores depended on the strain and time of incubation and the nutritious compounds used. The data obtained show that the amount of DPA released correlated to the number of A. acidoterrestris spores germinated

Diagnostyka i leczenie zachowawcze łokcia tenisisty = Diagnosis and conservative treatment of tennis elbow

Wójcik Gustaw, Stawińska Teresa, Szulc Anna, Sokołowska Barbara. Diagnostyka i leczenie zachowawcze łokcia tenisisty = Diagnosis and conservative treatment of tennis elbow. Journal of Education, Health and Sport. 2016;6(11):98-107. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.164191http://ojs.ukw.edu.pl/index.php/johs/article/view/3969   The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).755 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2016;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 22.10.2016. Revised 30.10.2016. Accepted: 31.10.2016.   DIAGNOSTYKA I LECZENIE ZACHOWAWCZE ŁOKCIA TENISISTYDIAGNOSIS AND CONSERVATIVE TREATMENT OF TENNIS ELBOW Wójcik Gustaw1,2, Stawińska Teresa3, Szulc Anna3, Sokołowska Barbara1  1 Państwowa Szkoła Wyższa im. Papieża Jana Pawła II w Białej Podlaskiej, Wydział Nauk o Zdrowiu i Nauk Społecznych.2 Uniwersytet Medyczny w Lublinie, Katedra Rehabilitacji, Fizjopterapii i Balneoterapii, Zakład Balneoterapii.3 Uniwersytet Medyczny w Lublinie, Katedra Rehabilitacji, Fizjoterapii i Balneoterapii, Zakład Rehabilitacji.   dr n. med. Wójcik Gustaw1,2dr n. o zdr. Stawińska Teresa3dr n. o zdr. Szulc Anna3dr n. med. Sokołowska Barbara1 Słowa kluczowe: łokieć tenisisty, nadkłykieć boczny, tendinopatie STRESZCZENIEWstęp Łokieć tenisisty jest stosunkowo częstym zaburzeniem układu mięśniowo-szkieletowego, dla którego skuteczna strategia leczenia jest nadal nieznana. Odpowiedzialny jest za znaczny ból i utratę funkcji chorej kończyny. Charakterystyczne dla schorzenia są degeneracyjne zmiany w strukturze kolagenu oraz nieprawidłowości w ukrwieniu związane z procesem neowaskularyzacji. Patologia jest wynikiem powtarzających się mikrourazów prowadzących do uszkodzenia tkanek. Czułość diagnostyki obrazowej zależna jest od czasu trwania schorzenia. Pierwszy okres choroby zazwyczaj wywoływany jest urazem, któremu może towarzyszyć stan zapalny, dlatego najbardziej skuteczną metodą leczenia może być terapia przeciwzapalna i unieruchomienie kończyny. Okres późniejszy to zmiany zwyrodnieniowe, a leczenie polega głównie na zwalczaniu bolesnych objawów. Stany oporne na leczenie zachowawcze kwalifikowane są do leczenia chirurgicznego.Cel pracy Przedstawienie aktualnej wiedzy na temat diagnostyki i leczenia entezopatii nadkłykcia bocznego kości ramiennej.Materiał i metody Praca oparta na przeglądzie piśmiennictwa z Baz PubMed, Scopus oraz Web of Science.Wnioski Podstawowym badaniem obrazowym w diagnostyce łokcia tenisisty jest badanie ultrasonograficzne. Klasyczna radiologia jest raczej wykorzystywana do różnicowania innych chorób dotyczących  łokcia. Współczesne metody leczenia łokcia tenisisty oparte są na terapii zmian zwyrodnieniowych. Leczenie przeciwzapalne jest nieskuteczne w powyższej jednostce chorobowej. Key words: tennis elbow, lateral epicondylitis, tendinopathy ABSTRACT Introduction. Tennis elbow is a relatively common disorder of the musculoskeletal system, for which effective treatment strategy is still unknown. It is responsible for considerable pain and loss of function of the affected limb. Characteristic for the diseases are degenerative changes in the structure of collagen and abnormalities in blood supply associated with the process of neovascularization. Pathology is due to cycles of repetitive stress leading to tissue damage. The sensitivity of imaging depends on the duration of the disease. The first period of the disease is usually caused by trauma, which may be accompanied by inflammation, which is why the most effective treatment may be anti-inflammatory therapy and immobilization of a limb. The subsequent period  is connected with degenerative changes, and treatment relies mainly on combating the painful symptoms. Conditions refractory to conservative treatment are eligible for surgery.The objective of the study. Presentation of current knowledge about diagnosis and treatment of enthesopathy of the lateral epicondyle of the humerus.Materials and method. The work is based on a review of literature of Baz PubMed, Scopus and Web of Science.Conclusions. The primary imaging technique for the diagnosis of tennis elbow is ultrasound. Classic radiology is rather used for the differentiation of other diseases related to the elbow. Modern methods of treatment of tennis elbow are based on the treatment of degenerative changes. Anti-inflammatory treatment is ineffective in the above disease

Analysis of selected factors predisposing to cardiovascular diseases in patients over 40 years old in districts of Biała Podlaska and Parczew

Wstęp. Choroby układu krążenia stanowią istotne zagrożenie zdrowia i życia. Odpowiadają one za 23% zgonów rocznie. Cel pracy. Ocena wybranych czynników predysponujących do wystąpienia chorób sercowo-naczyniowych u osób powyżej 40. roku życia. Materiał i metody. Badaniami objęto 205 mieszkańców powiatu bialskiego i parczewskiego. Narzędziem badawczym był autorski kwestiona­riusz ankiety. Wyniki. U 29,5% respondentów z powiatu bialskiego i 30% z powiatu parczewskiego rozpoznano otyłość. Aktywny tryb życia prowadziło 56,2% respondentów z powiatu bialskiego i 63% z powiatu parczewskiego; 22,9% badanych z powiatu bialskiego oraz 21% z powiatu parczewskiego nałogowo paliło papierosy. Tylko 1,9% mieszkańców powiatu bialskiego oraz 11% mieszkańców powiatu parczewskiego deklarowało częste spożywanie alkoholu. Wnioski. 1. Wśród czynników ryzyka wystąpienia chorób sercowo-naczyniowych u mieszkańców powiatu bialskiego i parczewskiego dominuje otyłość i nadwaga. 2. W grupie badanych konieczna jest modyfikacja trybu życia i sposobu odżywiania się oraz zwiększenie aktywności fizycznej i wyeliminowanie nałogu palenia tytoniu.Introduction. Cardiovascular diseases are a major threat to the health and life of the inhabitants of the world. In developed and developing countries they are responsible for 23% of deaths over the course of one year. Aim of the study. Evaluation of selected factors predisposing to cardiovascular diseases in people over 40 years of age. Material and methods. The survey was conducted among 205 people aged over 40, living in the district of Biała Podlaska and Parczew. A ques­tionnaire was used for the survey. Results. 29.5% of the respondents from Biała Podlaska region, and 30% of Parczew region were diagnosed with obesity. Keeping an active lifestyle was declared by 56.2% of respondents from Biała Podlaska region and 63% of Parczew region. 22.9% of respondents from Biała Podlaska region and 21% of Parczew region habitually smoke cigarettes. Only 1.9% of Biała Podlaska region, and 11% of the population of Parczew region admitted to frequent consumption of alcohol. Conclusions. 1. Amongst risk factors of the appearance of cardiovascular diseases in residents of Biała Podlaska region and Parczew region obe­sity and overweight dominate. 2. In the examined group an alteration of the lifestyle and eating habits is necessary as well as increasing physical activity and eliminating tobacco addiction

Assessment of workplace pathogens by the nursing staff : a research study

PURPOSE: The paper presents the results of an empirical study showing the current assessment of pathogens by nurses at their workplace. It identifies the problem areas in the professional work of the study group, including exposure to pathogens.DESIGN/METHODOLOGY/APPROACH: The study involved a group of 310 persons: nurses employed in various hospitals and medical institutions. The subjects were aged 23-50 years and above and had work experience ranging from 1 year of service to 35 years and above. The study used a diagnostic survey method based on a survey technique using a proprietary survey questionnaire. The research was administered in an online format in 2023.FINDINGS: There are pathogenic factors at nurses’ work including such factors as stress, work overload and biological factors. Regardless of the workplace, the vast majority of nursing staff described their exposure to harmful factors at very high levels. They showed a failure of active involvement and discussion concerning improving working conditions and a lack of conversation about pathogens.PRACTICAL IMPLICATIONS: The article presents the current knowledge of pathogenic factors in medical facilities and nurses' assessment of these factors. The factors mentioned in the article hinder the professional activity of medical personnel and may lead to the occurrence of diseases. The presented results from our own research may serve some initiative in initiating activity among this professional group, including a dialogue between the medical staff and management in the reduction of the listed factors. Furthermore, it may prompt a discussion on the problems diagnosed in the research. The lack of active involvement of nurses in improving working conditions should change.ORIGINALITY/VALUE: The authors present original research that has shown the threat from pathogenic factors in the workplace of medical staff, which significantly hinders professional activity and often causes illnesses and even results in a career change. The presentation of this topic is timely in view of the society expecting high quality medical services. The research conducted provides a basis for outlining new scientific challenges in nursing.peer-reviewe