Effect of training in SSG on the ability to repeat sprints in young football players

Introduction: Football is one of the most popular team sports worldwide. During a match, players perform varying acyclic activities at differing intensities in order to face the complex and dynamic competition requirements. For this reason, the aim of this study was demonstrate that training in small sided is an effective method for developing the ability to repeat sprints in young football players. Material and Method: Fifty-four male soccer players (U13, U15 and U17, n=18 in which group) at the youth level participated in this study. A program based on Small Sided Games (3vs3) during 6 weeks in a space of 30x25m was applied. A contrast of means, pre and post intervention, was carried out between control and the experimental group and segregating the sample by age group. Results: When analyzing the degree of relationship between RSA fatigue index and jump loss, no significant differences were found. We also studied the relationship between the jump power (CMJ) and the power of the best sprint performed at RSA. Discusion: These results are consistent with those obtained in previous studies (Clemente et al., 2014; Dellal et al., 2012; Owen et al., 2012) on elite players. Conclusion: It can be concluded that this program improves the capacity of recovery before high intensity efforts and allows, at the same time, to work on technical and tactical elements and to develop the physical profile of the players. This type of games provokes improvements in the vertical jump, generate more power in the (CMJ) and the capacity to repeat explosive efforts

Physiological Responses at 15 Minutes of Recovery after a Session of Functional Fitness Training in Well-Trained Athletes

The aim of this study was to analyse muscle fatigue and metabolic stress at 15 min of recovery after performing two independent sessions of functional fitness training (FFT): a session of strength functional fitness training (FFTstrength) and a session of endurance functional fitness training (FFTendurance). Methods: eighteen well-trained men conducted two protocols, separated by one week of rest: FFTstrength (3 sets of 21, 15 and 9 repetitions of Thruster with bar + Pull ups) and FFTendurance (3 sets × (30 kcal rowing + 15 kcal assault air bike)). Neuromuscular fatigue and metabolic stress were measured right before, right after and at 10 and 15 min after completing the FFT workout, as well as the mean heart rate (HRmean) and the rating of perceived exertion (RPE) at the end of the FFT. Results: FFTendurance recovered the velocity loss values after 15 min of recovery. On the other hand, FFTstrength only recovered velocity in the 1 m·s−1 Tests in squat (SQ), since the velocity levels were 7% lower in the 1 m·s−1 Tests in military press exercise (MP) after 15 min. Conclusions: These data indicate that there are specific recovery patterns not only as a function of the exercise and the body regions involved, but also regarding the recovery of neuromuscular and metabolic factors, since both FFT workouts obtained high blood lactate concentrations.Sección Deptal. de Radiología, Rehabilitación y Fisioterapia (Enfermería)Fac. de Enfermería, Fisioterapia y PodologíaTRUEAlfonso X el Sabio University Foundationpu

Do Totiviruses of the veil of Flor yeasts provide an evolutionary advantage?

The main objective of this work was to isolate and characterize viruses that infect both Saccharomyces and other Flor veil-forming genera and suggest the possible consequences they can have on the processes of formation of the Flor veil of typical D.O. Jerez-Xèrés-Sherry wines. Results obtained show that different Flor veil yeast strains isolated in Fino wines and in Amontillados presented a great resistance to ethanol and all of them presented infection by Totiviruses. These were identified after the extraction of double-stranded RNA and amplified by multiplex PCR with specific primers for the most common viruses involved in the killer factor. Subsequently, the same strains of both the S. cerevisiae species and Wickerhamomyces anomalus were subjected to an antiviral treatment with ribavirin. After microbiological analysis with Methylene Blue (MB) plates and by rt-PCR, it was determined that most of them did not show killer factor and the viruses involved in it had been correctly eliminated under the treatment with ribavirin. Subsequently, competition and implantation experiments of the treated and untreated yeasts were carried out against their controls. The wines inoculated with yeasts infected by Totiviruses showed to be dominant in all cases

Taxonomic singularities of the veil of Flor yeasts in Fino Wines from the D.O. Jerez-Xérèz-Sherry

The veil of “Flor yeasts” are responsible for the biological aging process of wines produced in the D.O. of Jerez and Sanlúcar de Barrameda (Finos and Manzanillas) in the province of Cádiz and Fino in the D.O. Montilla-Moriles in the province of Córdoba, as well as in other wine-growing regions of the world, such as France, South Africa, California, Sardinia or Hungary. The first attempts to classify these flor veil yeasts were based on biochemical assays of sugar assimilation and fermentation. Thus, it was concluded that the yeasts involved in biological aging belonged all to Saccharomyces cerevisiae, distinguishing four subspecies or physiological races: beticus, cheresiensis, montuliensis and rouxii. On the other hand, molecular techniques, which allow the characterization of oenological yeasts and discriminate between the different strains, are usually pulsed field electrophoresis (PFGE), the analysis of the polymorphism for the length of the restriction fragments (RFLP) of mitochondrial DNA, and multiplex PCR of microsatellites. Recent studies in our laboratory show the existence of at least 9 different genotypes of S. cerevisiae veil of flor species, based on the study of microsatellites (SSR-PCR). In addition, we have detected the presence of some species of non-Saccharomyces yeasts not described previously

Metodologías Activas para los “Cuidados de enfermería en el adulto con problemas en la eliminación urinaria y digestiva” de la asignatura Enfermería del Adulto III. Realidad Virtual para el marcaje del estoma

Dentro de la asignatura Enfermería de Adulto III hay un contenido denominado “Cuidados de enfermería en el adulto con problemas en la eliminación urinaria y digestiva”. El cuidado del estoma digestivo es una intervención principalmente de enfermería, por lo tanto, su formación y capacitación en este campo es imprescindible para garantizar la autonomía en los pacientes con estoma digestivo. Así pues, se hace necesaria la búsqueda y el establecimiento de las mejores prácticas pedagógicas para mejorar el aprendizaje y potenciar la formación de los alumnos de Grado en Enfermería para un desempeño autónomo y pertinente a las demandas sociales vinculadas al autocuidado de los pacientes con estoma digestivo. Por esta razón se elabora el presente proyecto, cuyo objetivo principal consiste en implementar las metodologías activas (MA) como estrategia didáctica para mejorar el nivel de enseñanza/aprendizaje en los estudiantes de Grado en Enfermería en el abordaje de las personas con estoma digestivo y analizar si estas metodologías mejoran su aprendizaje. Dentro de la gran variedad de métodos de aprendizaje activo, se ha seleccionado el Flipped Classroom (aula invertida), la Gamificación y la Realidad Virtual. El interés en estas MA se debe a su carácter innovador y por estar en pleno apogeo didáctico, además de los resultados relevantes mostrados en diferentes acciones formativas y los múltiples beneficios para el desarrollo del aprendizaje del alumno. Para alcanzar los objetivos propuestos, se organizará un seminario que se impartirá en los Campus Universitarios de Ceuta, Melilla y Granada. En una primera sesión se presentará el proyecto a los docentes encargados de la asignatura y se tendrá una primera toma de contacto con los alumnos, explicándoles cómo se va a desarrollar la acción formativa, así como la posibilidad de otorgar o no su consentimiento de participación. Tras finalizar la experiencia se llevará a cabo la evaluación de los resultados obtenidos, así como el impacto que han tenido las sesiones en los estudiantes a través de una encuesta de satisfacción

Effects of Creatine Supplementation after 20 Minutes of Recovery in a Bench Press Exercise Protocol in Moderately Physically Trained Men

The aims of this study were to analyse the effect of creatine supplementation on the performance improvement in a bench pressing (BP) strength test of muscle failure and to evaluate muscle fatigue and metabolic stress 20 min after the exercise. Methods: Fifty young and healthy individuals were randomly assigned to a creatine group (n = 25) or a placebo group (n = 25). Three exercise sessions were carried out, with one week of rest between them. In the first week, a progressive load BP test was performed until the individuals reached the one repetition maximum (1RM) in order to for us obtain the load-to-velocity ratio of each participant. In the second week, the participants conducted a three-set BP exercise protocol against 70% 1RM, where they performed the maximum number of repetitions (MNR) until muscle failure occurred, with two minutes of rest between the sets. After one week, and following a supplementation period of 7 days, where half of the participants consumed 0.3 g·kg−1·day−1 of creatine monohydrate (CR) and the other half consumed 0.3 g·kg−1·day−1 of placebo (PLA, maltodextrin), the protocol from the second week was repeated. After each set, and up to 20 min after finishing the exercise, the blood lactate concentrations and mean propulsive velocity (MPV) at 1 m·s−1 were measured. Results: The CR group performed a significantly higher number of repetitions in Set 1 (CR = 14.8 repetitions, PLA = 13.6 repetitions, p = 0.006) and Set 2 (CR = 8 repetitions, PLA = 6.7 repetitions, p = 0.006) after supplementation, whereas no significant differences were seen in Set 3 (CR = 5.3 repetitions, PLA = 4.7 repetitions, p = 0.176). However, there was a significant increase in blood lactate at minute 10 (p = 0.003), minute 15 (p = 0.020), and minute 20 (p = 0.015) after the exercise in the post-supplementation period. Similarly, a significant increase was observed in the MPV at 1 m·s−1 in the CR group with respect to the PLA group at 10, 15, and 20 min after the exercise. Conclusions: Although the creatine supplementation improved the performance in the strength test of muscle failure, the metabolic stress and muscle fatigue values were greater during the 20 min of recovery

Cellular and humoral immunogenicity of the mRNA-1273 SARS-CoV-2 vaccine in patients with hematologic malignancies

Recent studies have shown a suboptimal humoral response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) messenger RNA (mRNA) vaccines in patients diagnosed with hematologic malignancies; however, data about cellular immunogenicity are scarce. The aim of this study was to evaluate both the humoral and cellular immunogenicity 1 month after the second dose of the mRNA-1273 vaccine. Antibody titers were measured by using the Elecsys and LIAISON anti–SARS-CoV-2 S assays, and T-cell response was assessed by using interferon-γ release immunoassay technology. Overall, 76.3% (184 of 241) of patients developed humoral immunity, and the cellular response rate was 79% (184 of 233). Hypogammaglobulinemia, lymphopenia, active hematologic treatment, and anti-CD20 therapy during the previous 6 months were associated with an inferior humoral response. Conversely, age >65 years, active disease, lymphopenia, and immunosuppressive treatment of graft-versus-host disease (GVHD) were associated with an impaired cellular response. A significant dissociation between the humoral and cellular responses was observed in patients treated with anti-CD20 therapy (the humoral response was 17.5%, whereas the cellular response was 71.1%). In these patients, B-cell aplasia was confirmed while T-cell counts were preserved. In contrast, humoral response was observed in 77.3% of patients undergoing immunosuppressive treatment of GVHD, whereas only 52.4% had a cellular response. The cellular and humoral responses to the SARS-CoV-2 mRNA-1273 vaccine in patients with hematologic malignancies are highly influenced by the presence of treatments such as anti-CD20 therapy and immunosuppressive agents. This observation has implications for the further management of these patients.The authors also thank the Cellex Foundation for providing research facilities and equipment and the CERCA Programme/Generalitat de Catalunya for institutional support

Spontaneous reperfusion enhances succinate concentration in peripheral blood from stemi patients but its levels does not correlate with myocardial infarct size or area at risk

Cardiovascular biology; Diagnostic markers; Prognostic markersBiología cardiovascular; Marcadores de diagnóstico; Marcadores pronósticosBiologia cardiovascular; Marcadors diagnòstics; Marcadors pronòsticsSuccinate is enhanced during initial reperfusion in blood from the coronary sinus in ST-segment elevation myocardial infarction (STEMI) patients and in pigs submitted to transient coronary occlusion. Succinate levels might have a prognostic value, as they may correlate with edema volume or myocardial infarct size. However, blood from the coronary sinus is not routinely obtained in the CathLab. As succinate might be also increased in peripheral blood, we aimed to investigate whether peripheral plasma concentrations of succinate and other metabolites obtained during coronary revascularization correlate with edema volume or infarct size in STEMI patients. Plasma samples were obtained from peripheral blood within the first 10 min of revascularization in 102 STEMI patients included in the COMBAT-MI trial (initial TIMI 1) and from 9 additional patients with restituted coronary blood flow (TIMI 2). Metabolite concentrations were analyzed by 1H-NMR. Succinate concentration averaged 0.069 ± 0.0073 mmol/L in patients with TIMI flow ≤ 1 and was significantly increased in those with TIMI 2 at admission (0.141 ± 0.058 mmol/L, p < 0.05). However, regression analysis did not detect any significant correlation between most metabolite concentrations and infarct size, extent of edema or other cardiac magnetic resonance (CMR) variables. In conclusion, spontaneous reperfusion in TIMI 2 patients associates with enhanced succinate levels in peripheral blood, suggesting that succinate release increases overtime following reperfusion. However, early plasma levels of succinate and other metabolites obtained from peripheral blood does not correlate with the degree of irreversible injury or area at risk in STEMI patients, and cannot be considered as predictors of CMR variables. Trial registration: Registered at www.clinicaltrials.gov (NCT02404376) on 31/03/2015. EudraCT number: 2015-001000-58.This work was supported by the Spanish Ministry of Economy and Competitiveness, Instituto de Salud Carlos III (Grants PI17/01397 and CIBERCV) and the Spanish Society of Cardiology (Proyectos de la FEC para Investigación Básica en Cardiología 2018, Sociedad Española de Cardiología), and was cofinanced by the European Regional Development Fund (ERDF-FEDER, a way to build Europe). Antonio Rodríguez-Sinovas has a consolidated Miguel Servet contract