Association Of Nitric Oxide Synthase And Matrix Metalloprotease Single Nucleotide Polymorphisms With Preeclampsia And Its Complications.

Preeclampsia is one of the leading causes of maternal and neonatal morbidity and mortality in the world, but its appearance is still unpredictable and its pathophysiology has not been entirely elucidated. Genetic studies have associated single nucleotide polymorphisms in genes encoding nitric oxide synthase and matrix metalloproteases with preeclampsia, but the results are largely inconclusive across different populations. To investigate the association of single nucleotide polymorphisms (SNPs) in NOS3 (G894T, T-786C, and a variable number of tandem repetitions VNTR in intron 4), MMP2 (C-1306T), and MMP9 (C-1562T) genes with preeclampsia in patients from Southeastern Brazil. This prospective case-control study enrolled 77 women with preeclampsia and 266 control pregnant women. Clinical data were collected to assess risk factors and the presence of severe complications, such as eclampsia and HELLP (hemolysis, elevated liver enzymes, and low platelets) syndrome. We found a significant association between the single nucleotide polymorphism NOS3 T-786C and preeclampsia, independently from age, height, weight, or the other SNPs studied, and no association was found with the other polymorphisms. Age and history of preeclampsia were also identified as risk factors. The presence of at least one polymorphic allele for NOS3 T-786C was also associated with the occurrence of eclampsia or HELLP syndrome among preeclamptic women. Our data support that the NOS3 T-786C SNP is associated with preeclampsia and the severity of its complications.10e013669

PERFIL DE PRÁTICAS DOS PROFESSORES DE EDUCAÇÃO FÍSICA RELACIONADAS À MONTAGEM DE TREINOS NAS ACADEMIAS DE GINÁSTICA DE GOIÂNIA

A intervenção do professor de educação física nos diferentes espaços deveria ter como direção a formação de seres humanos críticos, independente do espaço no qual eles atuam. Apesar de haverem diferentes posições sobre as academias de ginástica as mesmas podem ser compreendidas como ambiente de educação não formal. Partindo desta premissa, questiona-se: Como os treinos são montados nas Academias de Ginástica de Goiânia? Partindo desta questão central, o presente trabalho tem como objetivo geral realizar um breve relato sobre as práticas que os professores de Educação Física se apropriam para prescrever treinos dentro das Academias de Ginástica de Goiânia. A metodologia adotada é o estudo descritivo, realizado através de entrevistas estruturadas em academias de ginástica de Goiânia

Antimicrobial activity and bioactive compounds of portuguese wild edible mushrooms methanolic extracts

The antimicrobial properties of phenolic extracts of Portuguese wild edible mushroom species (Lactarius deliciosus, Sarcodon imbricatus and Tricholoma portentosum) against pathogens were investigated. The minimal inhibitory concentrations (MICs) were evaluated for the entire mushroom, the cap and the stipe, separately; the portion of the mushroom used proved to be influenced in the results obtained, which are directly correlated with the content of total phenols and flavonoids in the extracts. The growth of Grampositive bacteria (Bacillus cereus, B. subtilis,) was well inhibited by these mushrooms, while Escherichia coli (Gramnegative bacteria) was resistant. The study on the antifungal effect of these mushrooms revealed that Candida albicans and Cryptococcus neoformans were differently inhibited for the mushrooms used

Angiogenesis‐related genes in endothelial progenitor cells may be involved in sickle cell stroke

The clinical aspects of sickle cell anemia (SCA) are heterogeneous, and different patients may present significantly different clinical evolutions.Almost all organs can be affected, particularly the central nervous system. Transient ischemic events, infarcts, and cerebral hemorrhage can be observed and affect ≈25% of the patients with SCA. Differences in the expression of molecules produced by endothelial cells may be associated with the clinical heterogeneity of patients affected by vascular diseases. In this study, we investigated the differential expression of genes involved in endothelial cell biology in SCA patients with and without stroke. Endothelial progenitor cells from 4 SCA patients with stroke and 6 SCA patients without stroke were evaluated through the polymerase chain reaction array technique. The analysis of gene expression profiling identified 29 differentially expressed genes. Eleven of these genes were upregulated, and most were associated with angiogenesis (55%), inflammatory response (18%), and coagulation (18%) pathways. Downregulated expression was observed in 18 genes, with the majority associated with angiogenesis (28%), apoptosis (28%), and cell adhesion (22%) pathways. Remarkable overexpression of the MMP1 (matrix metalloproteinase 1) gene in the endothelial progenitor cells of all SCA patients with stroke (fold change: 204.64; P+O46=0.0004) was observed. Our results strongly suggest that angiogenesis is an important process in sickle cell stroke, and differences in the gene expression profile of endothelial cell biology, especially MMP1, may be related to stroke in SCA patients9

Genotype frequencies of NOS3 VNTR in intron 4 for the study population.

<p>VNTR, variable number of tandem repetitions; ND, not determined</p><p>Genotype frequencies of NOS3 VNTR in intron 4 for the study population.</p

<i>Odds ratios</i> for risk factors for preeclampsia.

<p>CI, confidence interval; values in bold were statistically significant.</p><p><i>Odds ratios</i> for risk factors for preeclampsia.</p

Number of preeclamptic patients according to severity score and genotype distributions for <i>NOS3</i> T-786C.

<p>Number of preeclamptic patients according to severity score and genotype distributions for <i>NOS3</i> T-786C.</p