Could someone please drain my soils? I want to grow pulses

Non-Peer Reviewe

Candidate effectors contribute to race differentiation and virulence of the lentil anthracnose pathogen Colletotrichum lentis

RT-qPCR primers used to quantify expression of Colletotrichum lentis candidate effectors in an infection time-course on lentil cultivar Eston. (XLSX 10 kb

Resistance to Aphanomyces euteiches infection in wild lentil species

Non-Peer Reviewe

What in the soil is going on with prairie field pea production?

Non-Peer Reviewe

Sclerotinia stem rot control in small-seeded lentil production in the Black Soil Zone

Non-Peer ReviewedLentil production in the black soil zone is limited by the susceptibility of the crop to diseases such as sclerotinia stem rot. The objective of this project was to determine stem rot control strategies in small-seeded lentil by examining the impact of plant density, cultivar, fungicide treatment and timing of application on stem rot severity and yield. The relationship between flower petal infection and stem rot severity was also examined. Randomized complete block field experiments of three replicates were established at Rosthern and Melfort, SK and assessed for flower petal infection, disease severity and yield in 2000. Results of this single year of data indicated that fungicides reduced stem rot severity at both locations but increased yield only at Melfort, where later timing of application resulted in greater yield than early. Stem rot severity varied with cultivar however the cultivar with the greatest severity also had the greatest yield. Plant density had no effect on stem rot severity, but the lower plant density resulted in lower yield than the higher plant density. Flower petal infection was positively correlated with final disease rating only at Rosthern

Plant genomics in lentil breeding: development of a cDNA-based rapid screening method for Ascochyta blight resistance in lentil (Lens culinaris L.)

Non-Peer ReviewedAscochyta blight causes severe crop losses in temperate lentil production areas around the world, including Saskatchewan. At present there is no reliable system available to breeders for identifying blight resistant lentil lines. We are trying to develop a reliable, high throughput and low cost cDNA based system for screening lentil germplasm for polygenic resistance to Ascochyta blight. We have chosen the Ascochyta blight susceptible lentil variety Richlea and the closely related resistant breeding line 1156-2-17A for this study. Lentil plants were inoculated with spore suspensions of Ascochyta under conditions that clearly showed the difference in disease resistance between these lines. Tissue collected from resistant and susceptible lines at different times after inoculation will be used for extraction of total RNA–representing all the genes expressed by the plants in response to Ascochyta infection. Complementary DNA (cDNA) made from these RNA samples along with mock-inoculated controls will be visualized on poly acrylamide gels using the technique of ‘differential display’. We intend to identify the sequences of lentil genes expressed only by the resistant lines in response to Ascochyta infection and use them for developing molecular markers for the resistance trait. cDNA samples made from these plants will also be useful in developing a cDNA library of lentil tissue for future EST projects

Modifizierung von Platin-Oberflächen mittels Ionenstrahlen und deren elektrokatalytische Untersuchung

Die Modifizierung von Platinoberflächen unter Verwendung zweier ausgewählter Ionenstrahltechniken, mit dem Ziel, die elektrokatalytische Aktivität zu erhöhen. Mit Hilfe der Cyclovoltammetrie wurden die veränderten elektrokatalytischen Eigenschaften bei der Ameisensäureoxidationsreaktion untersucht

Reverse Genetics for Functional Genomics of Phytopathogenic Fungi and Oomycetes

Sequencing of over 40 fungal and oomycete genomes has been completed. The next major challenge in modern fungal/oomycete biology is now to translate this plethora of genome sequence information into biological functions. Reverse genetics has emerged as a seminal tool for functional genomics investigations. Techniques utilized for reverse genetics like targeted gene disruption/replacement, gene silencing, insertional mutagenesis, and targeting induced local lesions in genomes will contribute greatly to the understanding of gene function of fungal and oomycete pathogens. This paper provides an overview on high-throughput reverse genetics approaches to decode fungal/oomycete genomes

Evaluation of harvest aids application timing for lentil dry down

Non-Peer ReviewedHarvesting stage is a critical step for lentil producers to maintain high seed yield and good quality. Desiccating lentil with desiccants/harvest aids can dry down lentil evenly and quickly, and control late-growing green weeds, which enhances lentil harvest efficiency and allows early harvesting. Since the harvest aids are applied at a late growth stage, high herbicide residue in seeds may cause commercial issues with marketing lentil. Application timing of harvest aids is critical for producers. Improper application timing may reduce yield and thousand seed weight, but increase herbicide residue in seeds. Therefore, the objective of the harvest aids application timing (% seed moisture) trial was to evaluate the responses of lentil to different herbicide application timings at Saskatoon and Scott, Saskatchewan, over 2 years (2012 and 2013). For this trial, glyphosate (900 g a.e. ha-1), saflufenacil (50 g a.i. ha-1), and the combination of glyphosate plus saflufenacil (900 g a.e. ha-1 and 36 g a.i. ha-1) were applied when seed moisture content was 60%, 50%, 40%, 30% and 20%. Apart from these herbicide treatments, there was also an untreated control, which is desiccated naturally. Significant relationships between evaluated variables and application timing on the basis of seed moisture content were detected. Also, this trial indicated that early application timing (60% application seed moisture) could result in reductions in lentil yield and thousand seed weight. Glyphosate residue in seeds was less than 4 mg kg-1 when glyphosate was applied alone at 30% and 20% average seed moisture. Glyphosate residue decreased when adding saflufenacil to glyphosate. Saflufenacil residue consistently increased with earlier application timing of the harvest aids

Rethinking pulse crop production in the era of Aphanomyces root rot

Non-Peer Reviewe