531 research outputs found
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Complete Genome Sequence of Pseudomonas coronafaciens pv. oryzae 1_6
Pseudomonas coronafaciens pv. oryzae 1_6 was originally isolated as a phytopathogen of rice. Here, we report a complete genome sequence for this strain, containing a circular chromosome and one circular plasmid, assembled using a hybrid approach combining Illumina paired-end reads and longer reads sequenced on an Oxford Nanopore Flongle flow cell.Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
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Just the Two of Us? A Family of Pseudomonas Megaplasmids Offers a Rare Glimpse into the Evolution of Large Mobile Elements
Pseudomonads are ubiquitous group of environmental proteobacteria, well known for their roles in biogeochemical cycling, in the breakdown of xenobiotic materials, as plant growth promoters, and as pathogens of a variety of host organisms. We have previously identified a large megaplasmid present within one isolate of the plant pathogen Pseudomonas syringae, and here we report that a second member of this megaplasmid family is found within an environmental Pseudomonad isolate most closely related to Pseudomonas putida. Many of the shared genes are involved in critical cellular processes like replication, transcription, translation, and DNA repair. We argue that presence of these shared pathways sheds new light on discussions about the types of genes that undergo horizontal gene transfer (i.e., the complexity hypothesis) as well as the evolution of pangenomes. Furthermore, although both megaplasmids display a high level of synteny, genes that are shared differ by over 50% on average at the amino acid level. This combination of conservation in gene order despite divergence in gene sequence suggests that this Pseudomonad megaplasmid family is relatively old, that gene order is under strong selection within this family, and that there are likely many more members of this megaplasmid family waiting to be found in nature.US Department of Agriculture (USDA) [NIFA 2016-67014-24805]Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
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Complete Genome Sequence of Luteibacter pinisoli MAH-14
Diverse strains of Luteibacter (Gammaproteobacteria) have been isolated from a variety of environments, most frequently in association with both plants and fungi. Motivated by the lack of genomic information for strains throughout the genus Luteibacter, we report here a complete genome sequence for Luteibacter pinisoli strain MAH-14.Indigo AgricultureOpen access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
The shape of health to come: prospective study of the determinants of 30-year health trajectories in the Alameda County Study
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/57268/1/Kaplan GA et al The shape of health to come prospective study of the determinants of 30 year health trajectories in the Alameda County Study.pd
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A Novel, Highly Related Jumbo Family of Bacteriophages That Were Isolated Against Erwinia
Erwinia amylovora is a plant pathogen from the Erwiniaceae family and a causative agent of the devastating agricultural disease fire blight. Here we characterize eight lytic bacteriophages of E. amylovora that we isolated from the Wasatch front (Utah, United States) that are highly similar to vB_EamM_Ea35-70 which was isolated in Ontario, Canada. With the genome size ranging from 271 to 275 kb, this is a novel jumbo family of bacteriophages. These jumbo bacteriophages were further characterized through genomic and proteomic comparison, mass spectrometry, host range and burst size. Their proteomes are highly unstudied, with over 200 putative proteins with no known homologs. The production of 27 of these putative proteins was confirmed by mass spectrometry analysis. These bacteriophages appear to be most similar to bacteriophages that infect Pseudomonas and Ralstonia rather than Enterobacteriales bacteria by protein similarity, however, we were only able to detect infection of Erwinia and the closely related strains of Pantoea.United States Dairy Association; University of Arizona; Brigham Young University; Department of Microbiology and Molecular Biology; College of Life SciencesOpen access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
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Future-Proofing Your Microbiology Resource Announcements Genome Assembly for Reproducibility and Clarity.
Descriptions of resources, like the genome assemblies reported in Microbiology Resource Announcements, are often frozen at their time of publication, yet they will need to be interpreted in the midst of continually evolving technologies. It is therefore important to ensure that researchers accessing published resources have access to all of the information required to repeat, interpret, and extend these original analyses. Here, we provide a set of suggestions to help make certain that published resources remain useful and repeatable for the foreseeable future
Socioeconomic Position, Stress, and Cortisol in Relation to Waist Circumference in African American and White Women
Objective—Abdominal fat deposition has been shown to be related to hypertension,
dyslipidemia and diabetes. Studies have shown a correlation between cortisol (a stress hormone)
and abdominal fat deposition. Low socioeconomic position (SEP) has also been shown to be
related to abdominal fat deposition. It is hypothesized that chronic stress associated with low SEP
leads to high cortisol levels which in turn lead to abdominal fat deposition. Previous research in
this area has included mainly European subjects. The purpose of this study was to examine the
evidence for the SEP-chronic stress-cortisol-abdominal fat hypothesis in a sample of African
American and White American women.
Design—Data from the Regional Assessment Health Surveillance Study (RAHSS), a survey and
physical examination of a representative sample of African American and White adults residing in
six counties in Georgia, were utilized. The study population included 111 African American and
119 White women. Abdominal fat deposition was measured by waist circumference (inches).
Education and income were the measures of SEP. Other exposures examined included serum
cortisol, self-reported daily stress level, cigarette smoking, marital status, and number of children.
Associations were examined using multiple linear regression models adjusted for age and body
mass index (BMI).
Results—Among White women, less-educated women had a waist circumference 2.22 inches
larger (P<.05) than more highly educated women. Among African American women, separated or
divorced women (+2.29 in, P<.05) and widowed women (+3.13 in, P<.01) had larger waist
circumferences than married women. No other factors were significantly associated with waist
circumference. Conclusions—The SEP-chronic stress abdominal fat accumulation hypothesis was only
partially supported by the data. Different stressors and pathways may be important in producing
abdominal fat accumulation in African American and White women
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