1,004 research outputs found
Characterization of folding intermediates of a domain-swapped protein by solid-state NMR spectroscopy
Moleculare-level secondary structure, polymorphism, and dynamics of full-length alpha-synuclein fibrils studies by solid-state NMR
Π ΠΎΠ·ΡΠΎΠ±ΠΊΠ° ΠΌΠΎΠ±ΡΠ»ΡΠ½ΠΎΡ ΡΠ΅Ρ Π½ΠΎΠ»ΠΎΠ³ΡΡ ΡΠ΅ΠΌΠΎΠ½ΡΡ Π½ΠΈΠ·ΡΠΊΠΎΠ²ΡΠ³Π»Π΅ΡΠ΅Π²ΠΈΡ ΡΡΠ°Π»ΡΠ½ΠΈΡ ΡΡΡΠ± Π½ΠΈΠ·ΡΠΊΠΎΠ³ΠΎ ΡΠ° Π²ΠΈΡΠΎΠΊΠΎΠ³ΠΎ ΡΠΈΡΠΊΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΠ°ΠΉΠΊΠΈ Π½ΠΈΠ·ΡΠΊΠΎΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ½ΠΈΠΌΠΈ ΠΏΡΠΈΠΏΠΎΡΠΌΠΈ
Π ΠΎΠ·ΡΠΎΠ±Π»Π΅Π½ΠΎ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΡΡ Π±Π΅Π·Π²ΠΎΠ³Π½Π΅Π²ΠΎΠ³ΠΎ ΡΠ΅ΠΌΠΎΠ½ΡΡ Π΄Π΅ΡΠ΅ΠΊΡΡΠ² ΠΊΠΎΠ½ΡΡΡΡΠΊΡΡΠΉ Π· Π½ΠΈΠ·ΡΠΊΠΎΠ²ΡΠ³Π»Π΅ΡΠ΅Π²ΠΈΡ
ΡΡΠ°Π»Π΅ΠΉ ΡΠ»ΡΡ
ΠΎΠΌ ΠΏΠ°ΠΉΠΊΠΈ Π½ΠΈΠ·ΡΠΊΠΎΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ½ΠΈΠΌΠΈ ΠΏΡΠΈΠΏΠΎΡΠΌΠΈ. Π ΠΎΠ·ΡΠΎΠ±Π»Π΅Π½ΠΎ ΡΠ° Π·Π°ΡΠ²Π»Π΅Π½ΠΎ Π½ΠΎΠ²ΠΈΠΉ ΡΠΊΠ»Π°Π΄ Π»Π΅Π³ΠΊΠΎΠΏΠ»Π°Π²ΠΊΠΎΠ³ΠΎ ΠΏΡΠΈΠΏΠΎΡ Π½Π° Π±Π°Π·Ρ ΠΎΠ»ΠΎΠ²Π° Π· Π΄ΠΎΠΌΡΡΠΊΠ°ΠΌΠΈ ΠΌΡΠ΄Ρ ΡΠ° Π²ΡΡΠΌΡΡΡ. ΠΡΠΈΠΏΡΠΉ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΠΎ ΠΏΠΎΡΠ΄Π½ΡΡ Π²Π»Π°ΡΡΠΈΠ²ΠΎΡΡΡ ΡΡΠ΄ΠΊΠΎΠΏΠ»ΠΈΠ½Π½ΠΎΡΡΡ, ΠΊΠΎΡΠΎΠ·ΡΠΉΠ½ΠΎΡ ΡΡΡΠΉΠΊΠΎΡΡΡ, ΠΌΡΡΠ½ΠΎΡΡΡ ΡΠ° Π°Π΄Π³Π΅Π·ΡΠΉΠ½ΠΎΡ ΠΌΡΡΠ½ΠΎΡΡΡ. Π’Π΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΡΡ ΠΏΠ°ΠΉΠΊΠΈ ΡΡΠ°Π»Π΅ΠΉ Π½ΠΈΠ·ΡΠΊΠΎΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ½ΠΈΠΌΠΈ ΠΏΡΠΈΠΏΠΎΡΠΌΠΈ ΡΠ· Π·Π°ΡΡΠΎΡΡΠ²Π°Π½Π½ΡΠΌ Π²ΠΈΡΠΎΠΊΠΎΡΠ°ΡΡΠΎΡΠ½ΠΎΠ³ΠΎ Π³Π΅Π½Π΅ΡΠ°ΡΠΎΡΠ° Π΅Π»Π΅ΠΊΡΡΠΈΡΠ½ΠΎΠ³ΠΎ ΡΡΡΡΠΌΡ Π΄ΠΎΠ²Π΅Π΄Π΅Π½ΠΎ Π΄ΠΎ ΡΡΠ°Π½Ρ ΠΏΡΠ°ΠΊΡΠΈΡΠ½ΠΎΠ³ΠΎ Π·Π°ΡΡΠΎΡΡΠ²Π°Π½Π½Ρ.Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π° ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡ Π±Π΅Π·ΠΎΠ³Π½Π΅Π²ΠΎΠ³ΠΎ ΡΠ΅ΠΌΠΎΠ½ΡΠ° Π΄Π΅ΡΠ΅ΠΊΡΠΎΠ² ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΠΉ ΠΈΠ· Π½ΠΈΠ·ΠΊΠΎΡΠ³Π»Π΅ΡΠΎΠ΄ΠΈΡΡΡΡ
ΡΡΠ°Π»Π΅ΠΉ ΠΏΡΡΠ΅ΠΌ ΠΏΠ°ΠΉΠΊΠΈ Π½ΠΈΠ·ΠΊΠΎΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ½ΡΠΌΠΈ ΠΏΡΠΈΠΏΠΎΡΠΌΠΈ. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΠΈ Π·Π°ΡΠ²Π»Π΅Π½ Π½ΠΎΠ²ΡΠΉ ΡΠΎΡΡΠ°Π² Π»Π΅Π³ΠΊΠΎΠΏΠ»Π°Π²ΠΊΠΎΠ³ΠΎ ΠΏΡΠΈΠΏΠΎΡ Π½Π° Π±Π°Π·Π΅ ΠΎΠ»ΠΎΠ²Π°, Ρ Π΄ΠΎΠ±Π°Π²ΠΊΠ°ΠΌΠΈ ΠΌΠ΅Π΄ΠΈ ΠΈ Π²ΠΈΡΠΌΡΡΠ°. ΠΡΠΈΠΏΠΎΠΉ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΠΎ ΠΎΠ±ΡΠ΅Π΄ΠΈΠ½ΡΠ΅Ρ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΠΆΠΈΠ΄ΠΊΠΎΡΠ΅ΠΊΡΡΠ΅ΡΡΠΈ, ΠΊΠΎΡΡΠΎΠ·ΠΈΠΎΠ½Π½ΠΎΠΉ ΡΡΠΎΠΉ ΠΊΠΎΡΡΠΈ, ΠΏΡΠΎΡΠ½ΠΎΡΡΠΈ ΠΈ Π°Π΄Π³Π΅Π·ΠΈΠΎΠ½Π½ΠΎΠΉ ΠΏΡΠΎΡΠ½ΠΎΡΡΠΈ. Π’Π΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡ ΠΏΠ°ΠΉΠΊΠΈ ΡΡΠ°Π»Π΅ΠΉ Π½ΠΈΠ·ΠΊΠΎΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ½ΡΠΌΠΈ ΠΏΡΠΈΠΏΠΎΡΠΌΠΈ Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ Π²ΡΡΠΎΠΊΠΎΡΠ°ΡΡΠΎΡΠ½ΠΎΠ³ΠΎ Π³Π΅Π½Π΅ΡΠ°ΡΠΎΡΠ° ΡΠ»Π΅ΠΊΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΎΠΊΠ° Π΄ΠΎΠ²Π΅Π΄Π΅Π½ΠΎ Π΄ΠΎ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ.The technology of out fire defects repair in low carbon steel constructions by soldering with low- temperature solders is developed. The new fusible tin rich solder composition containing copper and bismuth additives is developed and declared. The solder has optimum properties of liquid stream, corrosion resistance, durability and adhesion strength. The low carbon steel soldering technology with application of high-frequency electric current generator is carried to practical application
Secondary chemical shifts in immobilized peptides and membrane proteins: A qualitative basis for structure refinement under Magic Angle Spinning.
ΠΡΠ΅Π΄ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²Π°Ρ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡ ΠΌΠΎΠ»ΠΎΡΠ½ΡΡ ΠΆΠ΅Π»Π΅Π· Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΡΡΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΠΎΠΏΡΠ»ΡΡΠΈΡΡ Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΠΡΡΠΌΠ°
Π‘Π΅ΡΠ΅Π΄ ΠΆΡΠ½ΠΎΡΠΎΠ³ΠΎ Π½Π°ΡΠ΅Π»Π΅Π½Π½Ρ ΠΠ Π Π²ΠΈΡΠ²Π»Π΅Π½Ρ ΠΏΠΎΠΏΡΠ»ΡΡΡΡ ΡΠ· ΡΠΊΡΠ°ΠΉ Π²ΠΈΡΠΎΠΊΠΎΡ
Π·Π°Ρ
Π²ΠΎΡΡΠ²Π°Π½ΡΡΡΡ Π ΠΠ (Π°ΡΠΌΡΠ½ΠΊΠΈ - 114,25); Π· Π²ΠΈΡΠΎΠΊΠΎΡ Π·Π°Ρ
Π²ΠΎΡΡΠ²Π°Π½ΡΡΡΡ (ΡΠ»ΠΎΠ²βΡΠ½ΠΊΠΈ -
65,21); Π° ΡΠ°ΠΊΠΎΠΆ Π· Π²ΡΠ΄Π½ΠΎΡΠ½ΠΎ Π½ΠΈΠ·ΡΠΊΠΎΡ Π·Π°Ρ
Π²ΠΎΡΡΠ²Π°Π½ΡΡΡΡ (ΠΊΡΠΈΠΌΡΡΠΊΡ ΡΠ°ΡΠ°ΡΠΊΠΈ - 41,99 Π½Π°
100 ΡΠΈΡ. Π²ΡΠ΄ΠΏΠΎΠ²ΡΠ΄Π½ΠΎΠ³ΠΎ ΠΆΡΠ½ΠΎΡΠΎΠ³ΠΎ Π½Π°ΡΠ΅Π»Π΅Π½Π½Ρ; Ρ < 0,001). ΠΠΎΡΡΠ²Π½ΡΠ»ΡΠ½ΠΈΠΉ Π°Π½Π°Π»ΡΠ·
ΠΏΠΎΡΠΈΡΠ΅Π½ΠΎΡΡΡ Π΄ΠΎΠ±ΡΠΎΡΠΊΡΡΠ½ΠΎΡ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΡΡ Π² 531 ΠΏΠ°ΡΡΡΠ½ΡΠΊΠΈ (399 ΡΠ»ΠΎΠ²βΡΠ½ΠΎΠΊ, 69 ΡΠ°ΡΠ°ΡΠΎΠΊ, 63
Π°ΡΠΌΡΠ½ΠΊΠΈ) ΠΏΠΎΠΊΠ°Π·Π°Π², ΡΠΎ Π²ΡΡΠΎΠ³ΡΠ΄Π½ΠΎ Π½Π°ΠΉΠ±ΡΠ»ΡΡ ΡΠ°ΡΡΠΈΠΌΠΈ Π·Π°Ρ
Π²ΠΎΡΡΠ²Π°Π½Π½ΡΠΌΠΈ Ρ Π²ΡΡΠΌΠ΅Π½ΡΡΠΊΡΠΉ
ΠΏΠΎΠΏΡΠ»ΡΡΡΡ Π· Π²ΠΈΡΠΎΠΊΠΎΡ Π·Π°Ρ
Π²ΠΎΡΡΠ²Π°Π½ΡΡΡΡ Π ΠΠ Ρ ΠΊΠΈΡΡΡ ΠΌΠΎΠ»ΠΎΡΠ½ΠΈΡ
Π·Π°Π»ΠΎΠ· (Π =0,033) Ρ
Π²ΡΠ·Π»ΠΎΠ²Π° ΡΠΎΡΠΌΠ° ΡΠΈΠ±ΡΠΎΠ·Π½ΠΎ-ΠΊΠΈΡΡΠΎΠ·Π½ΠΎΠΉ Ρ
Π²ΠΎΡΠΎΠ±ΠΈ (Π =0,040), ΡΠΊΡ, ΠΎΡΠ΅Π²ΠΈΠ΄Π½ΠΎ, ΠΌΠΎΠΆΠ½Π°
Π²ΡΠ΄Π½ΠΎΡΠΈΡΠΈ Π΄ΠΎ ΠΏΡΠ΅Π΄ΡΠ°ΠΊΠΎΠ²ΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΡΡ.In Crimean woman there are populations with very high Breast Cancer incidence (Armenians - 114,25); with high incidence (Slavs - 65,21); and with lowest incidence (Tatars - 41,99 in 100 000 woman populations; Ρ < 0,001). In 531 patients (399 Slavs, 69 Tatars, 63 Armenians) more freqwently was diagnosed Breast Cysts (Π =0,033) and Nodular Fibrocystic disease (Π =0,040) in Armenian ethnic group with very high Breast Cancer incidence. Breast Cysts and Nodular Fibrocystic disease only are precancerous diseases of the Breast
Solid-state NMR reveals structural differences between fibrils of wild-type and disease-related A53T mutant Ξ±-synuclein.
Inhibition of IGF1-R overcomes IGFBP7-induced chemotherapy resistance in T-ALL
Background T-cell acute lymphoblastic leukemia (T-ALL) is a genetically
heterogeneous disease with the need for treatment optimization. Previously,
high expression of Insulin-like growth factor binding protein 7 (IGFBP7), a
member of the IGF system, was identified as negative prognostic factor in
adult T-ALL patients. Since aberrant IGFBP7 expression was observed in a
variety of neoplasia and was relevant for prognosis in T-ALL, we investigated
the functional role of IGFBP7 in Jurkat and Molt-4 cells as in vitro models
for T-ALL. Methods Jurkat and Molt-4 cells were stably transfected with an
IGFBP7 over-expression vector or the empty vector as control. Proliferation of
the cells was assessed by WST-1 assays and cell cycle status was measured by
flow-cytometry after BrDU/7-AAD staining. The effect of IGFBP7 over-expression
on sensitivity to cytostatic drugs was determined in AnnexinV/7-AAD assays.
IGF1-R protein expression was measured by Western Blot and flow-cytometric
analysis. IGF1-R associated gene expression profiles were generated from
microarray gene expression data of 86 T-ALL patients from the Microarrays
Innovations in Leukemia (MILE) multicenter study. Results IGFBP7-transfected
Jurkat cells proliferated less, leading to a longer survival in a
nutrientβlimited environment. Both IGFBP7-transfected Jurkat and Molt-4 cells
showed an arrest in the G0/G1 cell cycle phase. Furthermore, Jurkat
IGFBP7-transfected cells were resistant to vincristine and asparaginase
treatment. Surface expression and whole protein measurement of IGF1-R protein
expression showed a reduced abundance of the receptor after IGFBP7
transfection in Jurkat cells. Interestingly, combination of the IGF1-R
inhibitor NPV-AEW541 restored sensitivity to vincristine in IGFBP7-transfected
cells. Additionally, IGF1-R associated GEP revealed an up-regulation of
important drivers of T-ALL pathogenesis and regulators of chemo-resistance and
apoptosis such as NOTCH1, BCL-2, PRKCI, and TP53. Conclusion This study
revealed a proliferation inhibiting effect of IGFBP7 by G0/G1 arrest and a
drug resistance-inducing effect of IGFBP7 against vincristine and asparaginase
in T-ALL. These results provide a model for the previously observed
association between high IGFBP7 expression and chemotherapy failure in T-ALL
patients. Since the resistance against vincristine was abolished by IGF1-R
inhibition, IGFBP7 could serve as biomarker for patients who may benefit from
therapies including IGF1-R inhibitors in combination with chemotherapy
Π‘ΡΡΡΠΊΡΡΡΠ½ΡΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ Π³Π΅ΠΌΠΎΡΡΠ°Π·Π° Ρ Π±ΠΎΠ»ΡΠ½ΡΡ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΠΎΠΉ ΠΊΠΎΠΆΠΈ Π½Π° ΡΠ°Π½Π½Π΅ΠΉ ΡΡΠ°Π΄ΠΈΠΈ Π΅Π΅ ΡΠ°Π·Π²ΠΈΡΠΈΡ
ΠΠ΅ΡΠ° Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ β ΠΎΡΡΠ½ΠΊΠ° ΠΏΠΎΠΊΠ°Π·Π½ΠΈΠΊΡΠ² ΡΠΈΡΡΠ΅ΠΌΠΈ Π³Π΅ΠΌΠΎΡΡΠ°Π·Ρ Ρ 27 Ρ
Π²ΠΎΡΠΈΡ
Π½Π° ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ ΡΠΊΡΡΠΈ. ΠΠΈΡΠ²Π»Π΅Π½ΠΎ ΠΏΠΎΠ΄ΠΎΠ²ΠΆΠ΅Π½Π½Ρ Π°ΠΊΡΠΈΠ²ΠΎΠ²Π°Π½ΠΎΠ³ΠΎ ΡΠ°ΡΡΠΊΠΎΠ²ΠΎΠ³ΠΎ ΡΡΠΎΠΌΠ±ΠΎΠΏΠ»Π°ΡΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΡΠ°ΡΡ, ΠΏΠΎΠ΄ΠΎΠ²ΠΆΠ΅Π½Π½Ρ ΡΠ°ΡΡΠΊΠΎΠ²ΠΎΠ³ΠΎ ΡΡΠΎΠΌΠ±ΠΎΠΏΠ»Π°ΡΡΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΡΠ°ΡΡ, ΠΏΠΎΠ΄ΠΎΠ²ΠΆΠ΅Π½Π½Ρ ΡΠ°ΡΡ ΡΠ΅ΠΊΠ°Π»ΡΡΠΈΡΡΠΊΠ°ΡΡΡ, Π·Π½ΠΈΠΆΠ΅Π½Π½Ρ ΡΡΠ²Π½Ρ Π°Π½ΡΠΈΡΡΠΎΠΌΠ±ΡΠ½Ρ III, ΠΏΡΠ΄Π²ΠΈΡΠ΅Π½Π½Ρ ΡΡΠ²Π½Ρ ΡΠΎΠ·ΡΠΈΠ½Π½ΠΈΡ
ΡΡΠ±ΡΡΠ½-ΠΌΠΎΠ½ΠΎΠΌΠ΅ΡΠ½ΠΈΡ
ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡΠ². Π’Π°ΠΊΠΈΠΌ ΡΠΈΠ½ΠΎΠΌ, ΠΌΠ°ΡΡΡ ΠΌΡΡΡΠ΅ ΡΠ΅ΡΠΉΠΎΠ·Π½Ρ ΡΡΡΡΠΊΡΡΡΠ½Ρ Π·ΠΌΡΠ½ΠΈ ΡΠΈΡΡΠ΅ΠΌΠ½ΠΎΠ³ΠΎ Π³Π΅ΠΌΠΎΡΡΠ°Π·Ρ - Π³ΡΠΏΠΎΠΊΠΎΠ°Π³ΡΠ»ΡΡΡΡ Ρ Π³ΡΠΏΠ΅ΡΡΡΠ±ΡΠΈΠ½ΠΎΠ»ΡΠ·. ΠΡΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ° ΡΠ° ΠΊΠΎΡΠ΅ΠΊΡΡΡ ΡΠΈΡ
Π·ΠΌΡΠ½ Π΄ΠΎΠΏΠΎΠΌΠΎΠΆΠ΅ ΠΏΠΎΠ»ΡΠΏΡΠΈΡΠΈ ΠΊΠ»ΡΠ½ΡΡΠ½ΠΈΠΉ ΠΏΠ΅ΡΠ΅Π±ΡΠ³ Ρ ΠΏΡΠΎΠ³Π½ΠΎΠ· Π·Π°Ρ
Π²ΠΎΡΡΠ²Π°Π½Π½Ρ.A research aim is an estimation of indexes of the system of hemostasis at 27 patients by the melanoma of skin. Lengthening of the activated partial tromboplastin time, lengthening of partial tromboplastin time, lengthening of time of recalcification, decline of level of antithrombin of III, increase of level of soluble fibrinmonomers complexes is educed. Thus, the serious structural changes in the system of hemostasis - incoagulability and hyperfibrinolysis take place. Diagnostics and correction of these changes will help to improve a clinical flow and prognosis of disease
Analysis of microsatellite instability in colorectal carcinoma by microfluidic-based chip electrophoresis
Microsatellite analysis is an important tool in clinical research and molecular diagnostics because microsatellite instability (MSI) occurs frequently in various types of cancer. Approximately 10β15% of colorectal, gastric and endometrial carcinomas are associated with MSI, and this has an impact on clinical prognosis. The microsatellite loci Bat25, Bat26, D2S123, D5S346 and D17S250, recommended by the Bethesda guidelines, were analysed by microfluidic-based on-chip electrophoresis in 40 cases of colon carcinoma with known MSI status. In all cases, microfluidic separation of the PCR amplicons resulted in highly resolved, distinct patterns of each of the five microsatellite loci. Detection of MSI could be demonstrated by microsatellite-loci-associated, well-defined deviations in the electropherogram profiles of tumour and non-tumour material, and confirmed the classification of MSI cases performed by conventional technology. In conclusion, microfluidic chip technology is a simple and reliable approach for MSI detection that allows label-free and very fast analysis of microsatellite amplicons
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