Histological and MRI brain atlas of the common shrew, Sorex araneus, with brain region-specific gene expression profiles

The common shrew, Sorex araneus, is a small mammal of growing interest in neuroscience research, as it exhibits dramatic and reversible seasonal changes in individual brain size and organization (a process known as Dehnel’s phenomenon). Despite decades of studies on this system, the mechanisms behind the structural changes during Dehnel’s phenomenon are not yet understood. To resolve these questions and foster research on this unique species, we present the first combined histological, magnetic resonance imaging (MRI), and transcriptomic atlas of the common shrew brain. Our integrated morphometric brain atlas provides easily obtainable and comparable anatomic structures, while transcriptomic mapping identified distinct expression profiles across most brain regions. These results suggest that high-resolution morphological and genetic research is pivotal for elucidating the mechanisms underlying Dehnel’s phenomenon while providing a communal resource for continued research on a model of natural mammalian regeneration. Morphometric and NCBI Sequencing Read Archive are available at https://doi.org/10.17617/3.HVW8ZN

Ensayo comparativo de rendimiento de maíz de siembra temprana : resultados de los ensayos realizados en la Unidad Demostrativa Agrícola de INTA San Antonio de Areco, campaña 2020/2021

El objetivo de este trabajo es la identificación de híbridos comerciales de maíz de genética moderna, estables y con alto potencial de rendimiento en el área de influencia de la localidad de San Antonio de Areco (norte de la provincia de Buenos Aires) que ayude a asesores y productores en la elección de los materiales. La elección de un cultivar depende de la consideración de un conjunto de características: ciclo, velocidad de secado de grano, comportamiento sanitario, resistencia al quebrado y al vuelco de la caña, rendimientos y la relación precio/rentabilidad. Este informe pretende ser una herramienta para colaborar en la toma de decisiones del sector productivo.EEA PergaminoFil: Jecke, Fernando Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural San Antonio de Areco; ArgentinaFil: Mousegne, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural San Antonio de Areco; ArgentinaFil: Paolilli, María Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Desarrollo Rural; ArgentinaFil: Neselis, Mariano. Universidad del Salvador - USAL; ArgentinaFil: Baldoni, César. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural Rojas; Argentin

Histological and MRI brain atlas of the common shrew, Sorex araneus, with brain region-specific gene expression profiles

The common shrew, Sorex araneus, is a small mammal of growing interest in neuroscience research, as it exhibits dramatic and reversible seasonal changes in individual brain size and organization (a process known as Dehnel’s phenomenon). Despite decades of studies on this system, the mechanisms behind the structural changes during Dehnel’s phenomenon are not yet understood. To resolve these questions and foster research on this unique species, we present the first combined histological, magnetic resonance imaging (MRI), and transcriptomic atlas of the common shrew brain. Our integrated morphometric brain atlas provides easily obtainable and comparable anatomic structures, while transcriptomic mapping identified distinct expression profiles across most brain regions. These results suggest that high-resolution morphological and genetic research is pivotal for elucidating the mechanisms underlying Dehnel’s phenomenon while providing a communal resource for continued research on a model of natural mammalian regeneration. Morphometric and NCBI Sequencing Read Archive are available at https://doi.org/10.17617/3.HVW8ZNpublishe

Avances en la adopción de buenas prácticas agrícolas y manejo integrado de plagas en quintas hortícolas de Baradero (Bs.As.)

Con el fin de incentivar a ocho productores de hortalizas en la implementación de BPA y evaluación del impacto ambiental, económico y social de sus producciones se ejecutó en conjunto entre INTA y SENASA el Plan Piloto “Horticultura Responsable”. Desde agosto del año 2015 se organizaron capacitaciones y encuentros en las quintas; los productores realizaron un registro de las labores y agroquímicos utilizados; se analizó la calidad agronómica del suelo y el agua, así como la carga microbiológica del agua de consumo y riego; también se monitoreó los residuos de plaguicidas y contaminación microbiológica de las hortalizas obtenidas. Se diseñaron afiches, volantes y etiquetas para cada productor, siguiendo los requisitos de la normativa. Los productores se inscribieron en el Renspa y mejoraron la presentación de sus productos utilizando envases de plástico y una heladera exhibidora. La iniciativa contó con el apoyo del Programa Cambio Rural y de Pro Huerta, a través de un Proyecto Especial que ha financiado inversiones para avanzar con BPA y MIP y la compra de una chipeadora para mejorar la calidad de los suelos a partir de la incorporación de materia orgánica. En la campaña 2017/2018 los productores experimentaron el monitoreo de plagas y enfermedades, el uso de trampas de feromonas para el control de polilla de tomate, la utilización de aceite de neem y la técnica de biosolarización. Finalizada esta experiencia decidieron adoptar el trampeo masivo para reducir el uso de plaguicidas en el cultivo de tomate realizando una compra en conjunto de los insumos necesarios.EEA San PedroFil: Mitidieri, Mariel Silvina . Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Delprino, María Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Fortunato, Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Pagliaricci, Leandro Osvaldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Fernández, Analía. Senasa. Servicio Nacional de Sanidad y Calidad Agroalimentaria; ArgentinaFil: Sampietro, Esteban. Senasa. Servicio Nacional de Sanidad y Calidad Agroalimentaria; ArgentinaFil: D´Angelcola, María Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Gerencia de Control de Gestión; ArgentinaFil: Cuellas, Marisol Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Área Metropolitana de Buenos Aires; ArgentinaFil: Valenzuela, Osvaldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Bernardez, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Piris, Estela Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Barbieri, Martín Osvaldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Brambilla, María Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Del Pardo, Cecilia Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Ciaponi, Mirta Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Taquichiri, E. GAL Altiplano. Baradero; ArgentinaFil: Aricoma, Alfredo. GAL Altiplano. Baradero; ArgentinaFil: Yucra, Mario. GAL Altiplano. Baradero; ArgentinaFil: Yucra, Franco. GAL Altiplano. Baradero; ArgentinaFil: Calva, L. GAL Altiplano. Baradero; ArgentinaFil: Sánchez, María Florencia. ProHuerta-GAL; ArgentinaFil: Heguiabeheri, Adolfo Ricardo. Secretaría de Agroindustria; ArgentinaFil: Baldoni, César Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Agencia de Extensión Rural Rojas; ArgentinaFil: Piola, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Peña, Lorena Claudina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentin

Factors Affecting the Regeneration, via Organogenesis, and the Selection of Transgenic Calli in the Peach Rootstock Hansen 536 (Prunus persica × Prunus amygdalus) to Express an RNAi Construct against PPV Virus

Prunus spp. is one of the most recalcitrant fruit tree species in terms of in vitro regeneration and transformation, mostly when mature tissues are used as explants. The present study describes the in vitro regeneration via indirect organogenesis, and Agrobacterium tumefaciens-mediated transformation of the peach rootstock Hansen 536 (Prunus persica × Prunus amygdalus) through the use of meristematic bulks (MBs) as starting explants. Efficient adventitious shoot regeneration was obtained when Hansen 536 MBs were cultured on an optimized medium consisting of modified McCown Woody Plant medium (WPM) enriched with 4.4 μM 6-Benzyladenine (BA), 0.1 μM 1-Naphthaleneacetic acid (NAA) and 6.0 g L−1 plant agar S1000 (B&V). MB slices were used later as starting explants for Agrobacterium-mediated transformation to introduce an RNAi construct “ihp35S-PPV194” against PPV virus. Transgenic events were identified by both green fluorescent protein (GFP) screening and kanamycin selection at different concentrations (0, 17 or 42 μM). GFP-fluorescent proliferating callus lines were selected and confirmed to stably express the ihp35S-PPV194::eGFP gene construct by molecular analysis. Although shoot regeneration from these transgenic calli has not been obtained yet, this represents one of the few examples of successful attempts in peach genetic transformation from somatic tissues, and also serves as a useful in vitro system for future gene functional analysis in peach

Impact of Bioaugmentation on the Bioremediation of Saline-Produced Waters Supplemented with Anaerobic Digestate

Bioremediation of produced waters has been widely investigated in the last decades. More recently, microalgae-based treatments have been developed to produce biomass. The objective of this study was to determine, at lab scale, the remediation efficiency of three origin of microorganisms: a consortium of three halotolerant and halophilic microalgae and their associated bacteria, bacteria from liquid digestate, and aromatic-degrading bacteria selected to perform bioaugmentation. The medium was composed of artificial oil-produced water and seawater, and contained nutrients from liquid digestate. In order to identify what plays a role in nitrogen, chemical oxygen demand, and aromatics compounds elimination, and to determine the effectiveness of bioaugmentation to treat this mix of waters, 16S rRNA analyses were performed. Combination of microorganisms from different origins with the selected aromatic-degrading bacteria were also realized, to determine the effectiveness of bioaugmentation to treat these waters. Each population of microorganisms achieved similar percentage of removal during the biological treatment, with 43–76%, 59–77%, and 86–93% of elimination for ammonium, chemical oxygen demand, and aromatic compounds (with 50% of volatilization), respectively, after 7 days, and up to with 100%, 77%, and 99% after 23 days, demonstrating that in the case of this produced water, bioaugmentation with the specialized aromatic-degrading bacteria had no significant impact on the treatment. Regarding in detail the populations present and active during the tests, those from genus Marinobacter always appeared among the most active microorganisms, with some strains of this genus being known to degrade aromatic compounds

Intra- and interspecific variation in self-control capacities of parrots in a delay of gratification task

Forgoing immediate satisfaction for higher pay-offs in the future (delayed gratification) could be adaptive in situations that wild animals may encounter. To explain species-differences in self-control, hypotheses based on social complexity, feeding ecology, brain size and metabolic rate have been proposed. To explore these hypotheses in a comparative setting, we tested three macaw species (neotropical parrots)-great green macaws (N = 8), blue-throated macaws (N = 6), blue-headed macaws (N = 6)-and the distantly related African grey parrots (afrotropical parrots; N = 8) in a modified rotating tray task, in which subjects are required to inhibit consuming a constantly available low-quality reward in favour of a high-quality reward that becomes available only after an increasing delay (min. 5 s, max. 60 s). All four species successfully waited for a minimum of 8.3 s ± 11.7 s (group level mean ± SD) with African greys reaching a delay of 29.4 ± 15.2 s, and great green macaws-as best performing macaw species-tolerating delays of 20 s ± 8 s. The best performing African grey individual reached a maximum delay of 50 s, whereas, a great green and a blue-throated macaw tolerated a delay of 30 s max. Females tolerated higher maximum delays than males. Engaging in distraction behaviours enhanced waiting performance across species and all birds were able to anticipate the waiting duration. Our results suggest that both feeding and socio-ecological complexity may be a factor in self-control, but further systematically collected comparative data on self-control of different (parrot) species are required to test the evolutionary hypotheses rigorously.publishe

Cross-front variations in adult abundance and recruitment of Patagonian scallop (Zygochlamys patagonica) at the SW Atlantic Shelf Break Front

We studied cross-front variations in adult abundance and recruitment of Patagonian scallop ( Zygochlamys patagonica ) and their relationship with the Shelf Break Front and satellite-derived chlorophyll a in the Southwest Atlantic between 38 8 S and 39 8 30 0 S. Integrated data from commercial fleets (CF, 1996–2005), research cruises (RC, 1998–2005), sea surface temperature (SST; 1985– 1997), and satellite-derived chlorophyll a (CSAT; 1998–2004) were included in the analysis. One-way ANOVA was used to assess differ- ences in recruitment and scallop abundance in a cross-front direction. The abundance of scallops was greatest (RC . 200 kg h 2 1 ) closer to the onshore side of the front or beneath it, and the East–West extension of scallop beds ( 40 km) matched the seasonal zonal displacement of the front ( 37 km). The highest CSAT gradients were west of or matched the position of the front. The annual mean position of the front correlated with the spatial variability in recruitment within areas where the species recruited successfully (RC . 2000 h 2 1 ). The spatial variability in adult abundance and recruitment of scallops is strongly related to the spatial variability in the position of the front and with the high CSAT concentrations associated with it.Fil: Mauna, Andrea Cecilia. Instituto Nacional de Investigaciones y Desarrollo Pesquero; ArgentinaFil: Franco, Barbara Cristie. Ministerio de Defensa. Armada Argentina. Servicio de Hidrografía Naval. Centro Argentino de Oceanografía; ArgentinaFil: Baldoni, Ana. Instituto Nacional de Investigaciones y Desarrollo Pesquero; ArgentinaFil: Acha, Eduardo Marcelo. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Lasta, Mario L.. Instituto Nacional de Investigaciones y Desarrollo Pesquero; ArgentinaFil: Iribarne, Oscar Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentin

Lenguajes I. Cuadernos de cátedra I : escrituras, análisis y experiencias socio-semióticas

La edición de este conjunto de textos en formato libro –no impreso, digital– reúne una gran parte de la experiencia educativa que llevamos adelante docentes y estudiantes de la materia Lenguajes I durante los últimos años. El desarrollo de las clases y la elaboración sucesiva de diferentes programas de aprendizaje cimentan estas escrituras que traen los ecos dialógicos de una conversación que se inició en el momento fundacional de la carrera en “Comunicación Social” en 1974, dos años antes del inicio de la fatídica dictadura cívico-militar que asoló a nuestro país entre el 24 de marzo de 1976 y el 10 de diciembre de 1983 y que, entre otras consecuencias nefastas, produjo el cierre de la licenciatura durante un año.Fil: Echecopar, Cecilia. Universidad Nacional de Rosario. Facultad de Ciencia Política y Relaciones Internacionales. Escuela de Comunicación Social; Argentina

Histological and MRI brain atlas of the common shrew, Sorex araneus, with brain region-specific gene expression profiles

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