FSTL5 expression is a marker of Group C metastatic medulloblastomas

INTRODUCTION: Medulloblastoma (MB) is the most commonmalignant brain tumor in children. Four different molecular subgroups are recognized, which differ in gene expression, genomic aberrations, histology, demographics and survival:WNT and SHH groups, having specific mutations in the homonymous pathway, and groups C and D having several genetic alternations not specific to a single pathway. The gene for follistatin-like protein 5, FSTL5, is overexpressed in nonSHH/nonWNT MBs poorly characterized. Highexpression of FSTL5 is significantly associated with reduced event-free and overall survival in non-WNT/non-SHHMBs. The major aim of this project is to study the FSTL5 expression level in pediatric MBs with metastasis at the onset. METHOD: We investigated the protein expression of biomarkers involved in metastatic pathways by IHC and FSTL5 expression level by RT-PCR in 26 metastatic MBs samples and correlated these data with the outcomes by Kaplan-Meier statistic analysis. RESULTS: 83% of Group C MBs showed high level of FSTL5 while none of these presented down-expression. Low-expression level of FSTL5 was find in 60% of SHH MBs and none showed over-expression. Kaplan-Meier test revealed that, in our cohort, highexpression ofFSTL5didnot correlatewithworse outcomewhile lowexpression of FSTL5 was associated with good prognosis and the co-presence of FSTL5 with other biomarkers correlated with poorer prognosis. CONCLUSION: FSTL5 is a marker of Group C in medulloblastomas with metastasis at the onset and the results highlighted decreased FSTL5 expression as a marker of good prognosis. Group C MBs have characteristic molecular features that confirm the poorest outcome also inMBs with metastasis at the onset

Current Status of Poultry Meat Abnormalities

Over the past decade, the poultry industry has faced an increasing occurrence of growth-related muscular abnormalities that mainly affect fast-growing genotypes selected for their production performances (high growth rate and breast yield). These abnormalities, termed white striping (WS), wooden breast (WB) and spaghetti meat (SM), primarily affect the superficial portion of pectoralis major muscles. Despite their distinctive phenotypes, WS, WB, and SM conditions entail common histological features, i.e., they might share common causative mechanisms underpinning their occurrence. Meat affected by growth-related abnormalities is harmless for human nutrition since no specific biological or chemical hazards have been found to be related to its consumption. However, WS, WB, and SM abnormalities negatively affect both quality traits and technological properties of raw and processed meat, causing relevant economic damages in the poultry industry. This paper aims to provide an update about the current status of poultry meat abnormalities, giving useful insights about their impact on meat quality, the possible causative mechanisms, methods for mitigation, and future perspectives

Spaghetti Meat Abnormality in Broilers: Current Understanding and Future Research Directions

open3noSpaghetti meat (SM) is a recent muscular abnormality that affects the Pectoralis major muscle of fast-growing broilers. As the appellative suggests, this condition phenotypically manifests as a loss of integrity of the breast muscle, which appears soft, mushy, and sparsely tight, resembling spaghetti pasta. The incidence of SM can reach up to 20% and its occurrence exerts detrimental effects on meat composition, nutritional value, and technological properties, accounting for an overall decreased meat value and important economic losses related to the necessity to downgrade affected meats. However, due to its recentness, the causative mechanisms are still partially unknown and less investigated compared to other muscular abnormalities (i.e., White Striping and Wooden Breast), for which cellular stress and hypoxia caused by muscle hypertrophy are believed to be the main triggering factors. Within this scenario, the present review aims at providing a clear and concise summary of the available knowledge concerning SM abnormality and concurrently presenting the existing research gaps, as well as the potential future developments in the field.openBaldi, Giulia; Soglia, Francesca; Petracci, MassimilianoBaldi, Giulia; Soglia, Francesca; Petracci, Massimilian

Sobolev-Gaffney type inequalities for differential forms on sub-Riemannian contact manifolds with bounded geometry

In this article, we establish a Gaffney type inequality, in Wℓ,p -Sobolev spaces, for differential forms on sub-Riemannian contact manifolds without boundary, having bounded geometry (hence, in particular, we have in mind noncompact manifolds). Here, p∈]1,∞[ and ℓ=1,2 depending on the order of the differential form we are considering. The proof relies on the structure of the Rumin’s complex of differential forms in contact manifolds, on a Sobolev-Gaffney inequality proved by Baldi-Franchi in the setting of the Heisenberg groups and on some geometric properties that can be proved for sub-Riemannian contact manifolds with bounded geometry

Nutritional behavior and attitudes in food allergic children and their mothers

BACKGROUND: Avoidance of food allergens requires adapting dietetic habits, changing nutritional approach. A restriction of food choice can result in a monotonous diet and impact social life. This study investigated the impact of food allergy on nutritional behavior and attitudes of patients and their families. METHODS: A survey involving mothers of food allergic children aged 0–16 years was carried out. We primarily studied the variables related to the child (age, gender, clinical history, food and social events attitudes). In addition, Spielberg Trait-Anxiety Inventory (STAI-T) test was applied to the mothers. We assessed separately the associations between characteristics of child-mother pairs and diet monotony, and attendance to social events, by means of proportional odds regression models. RESULTS: Nearly 10% of the 124 participants completely banned allergenic foods at home and 15.3% consumed their meals separately. More than one fourth attended parties rarely or never. Most of the participants reported a “monotonous diet”. Model results suggested significant associations between child age (p = 0.05), mother age (p = 0.05), number of excluded foods (p = 0.003) and monotony of the diet. The attendance of social events was inversely associated with the number of excluded foods (p = 0.04) and the mother’s STAI-T T-score (p = 0.04). CONCLUSIONS: The results highlighted the impact of food allergy in reducing interest about food and influencing patients’ approach to social life. It is important to support families in managing allergens avoidance

The role of histidine dipeptides on postmortem acidification of broiler muscles with different energy metabolism

It is generally held that the content of several free amino acids and dipeptides is closely related to the energy-supplying metabolism of skeletal muscles. Metabolic characteristics of muscles are involved in the variability of meat quality due to their ability to influence the patterns of energy metabolism not only in living animal but also during post-mortem time. Within this context, this study aimed at establishing whether the concentration of histidine dipeptides can affect muscle post-mortem metabolism, examining the glycolytic pathway of three chicken muscles (Pectoralis major, extensor iliotibialis lateralis and gastrocnemius internus as glycolytic, intermediate and oxidative-type, respectively) selected based on their histidine dipeptides content and ultimate pH. Thus, a total of 8 carcasses were obtained from the same flock of broiler chickens (Ross 308 strain, females, 49 days of age, 2.8 kg body weight at slaughter) and selected immediately after evisceration from the line of a commercial processing plant. Meat samples of about 1 cm3 were excised from bone-in muscles at 15, 60, 120 and 1,440 min post-mortem, instantly frozen in liquid nitrogen and used for the determination of pH, glycolytic metabolites, buffering capacity as well as histidine dipeptides content through 1H-NMR. Overall results suggest that glycolysis in leg muscles ceased already after 2 h post-mortem, while in breast muscle continued until 24 h, when it exhibited significantly lower pH values (P<0.05). However, considering its remarkable glycolytic potential, Pectoralis major muscle should have exhibited a greater and faster acidification, suggesting that its higher (P<0.05) histidine dipeptides\u2019 content might have prevented a potentially stronger acidification process. Accordingly, breast muscle also showed greater (P<0.05) buffering ability in the pH range 6.0-7.0. Therefore, anserine and carnosine, being highly positively correlated with muscle\u2019s buffering capacity (P<0.001), might play a role in regulating post-mortem pH decline, thus exerting an effect on muscle metabolism during pre-rigor phase and the quality of the forthcoming meat. Overall results also suggest that total histidine dipeptides content along with muscular ultimate pH represent good indicators for the energy-supplying metabolism of chicken muscles

Technical note: Estimation of real rabbit meat consumption in Italy

[EN] As in other livestock species, the annual per capita consumption of rabbit meat is currently estimated as the ratio of the total weight of carcasses available for consumption to the number of inhabitants of a certain region. The aim of this work was to establish conversion coefficients from carcass to dible lean meat and estimate real rabbit meat consumption in Italy. Accordingly, a total of 24 rabbits were slaughtered at 2 different ages to obtain carcasses representative of the main market categories in Northern Italy: medium-size (carcass weight of about 1.4 kg) and heavy-size (carcass weight of about 1.8 kg). Chilled carcasses were used to determine offal, dissectible fat, bone and meat weights and yields. Experimentally obtained conversion factors from carcass to edible lean meat and estimated meat waste percentage at retail and consumption levels were subsequently used to estimate the real per capita amount of rabbit meat consumed in Italy. The finding of this study revealed that, if compared to the medium-size group, heavy-size carcasses had higher lean meat yield for both intermediate (92.9 vs. 92.4%; P<0.05) and hind parts (84.3 vs. 79.1%; P<0.001). On the contrary, the meat yield of fore part was higher in the medium-size group (66.2 vs. 65.5%; P<0.001) compared to heavy-size carcasses. Eventually, overall meat yield was higher in heavy-size carcasses compared to medium-size ones (64.4 vs. 63.2%; P<0.001). By using these conversion factors and estimated overall losses at retailing and home-consumption (15%), we estimated that real per capita annual rabbit meat consumption is 0.50 kg in Italy, which is only 54% compared to the estimated apparent consumption (0.90 kg).Petracci, M.; Soglia, F.; Baldi, G.; Balzani, L.; Mudalal, S.; Cavani, C. (2018). Technical note: Estimation of real rabbit meat consumption in Italy. World Rabbit Science. 26(1):91-96. doi:10.4995/wrs.2018.7802SWORD919626

Exergy Recovery During Liquefied Natural Gas Regasification Using Methane as Working Fluid

The increased concerns about the effect of human activities on the climate have pushed natural gas among the most obvious solutions for the transition to a low-carbon economy. The growing importance and volumes of liquefied natural gas for transportation over long distances come as a consequence of this tendency. The liquefaction of natural gas requires a high amount of energy that can be recovered during the re-gasification phase. In this paper, a novel approach for this purpose is presented, where the main feature is the use of a combination of Rankine and Brayton cycles while retaining natural gas as the only working fluid of the system. The proposed system is optimized for cost and exergy efficiency using a bi-level multi-objective optimization procedure, where the master level is setup as a nonlinear optimization problem and solved using an evolutionary algorithm, while the slave level as a mixed integer-linear programming problem. The results of the optimization show that such system can potentially achieve high efficiencies (up to 60 % exergy efficiency for the power cycle and above 65 % plant thermal efficiency), at the cost of a significant capital investment for the heat exchanger network. By allowing a lower level of integration in the system a profitability of up to 98 kUSD/y can be achieved, while retaining significantly high performance

Uncovering the potential of blockchain in the agri-food supply chain. An interdisciplinary case study

This paper explores how Blockchain technology (BCT) can be integrated in the agri-food supply chain (ASC) and how BCT-based networks are formed. To do this, the paper describes a BCT solution, designed to enhance traceability, and analyses its adoption in two small firms. Adopting an interdisciplinary approach and the Actor-Network Theory (ANT), the findings have revealed that BCT improves how data is collected and has changed how firms interact with stakeholders and customers. Firms have enhanced their reputations and started targeting new domestic and international markets. Technical and economic challenges were found when persuading actors to participate in the BCT-based network