Ecological speciation in sympatric palms: 2. Pre- and post-zygotic isolation

We evaluated reproductive isolation in two species of palms (Howea) that have evolved sympatrically on Lord Howe Island (LHI, Australia). We estimated the strength of some pre- and post-zygotic mechanisms in maintaining current species boundaries. We found that flowering time displacement between species is consistent across in and ex situ common gardens and is thus partly genetically determined. On LHI, pre-zygotic isolation due solely to flowering displacement was 97% for Howea belmoreana and 80% for H. forsteriana; this asymmetry results from H. forsteriana flowering earlier than H. belmoreana and being protandrous. As expected, only a few hybrids (here confirmed by genotyping) at both juvenile and adult stages could be detected in two sites on LHI, in which the two species grow intermingled (the Far Flats) or adjacently (Transit Hill). Yet, the distribution of hybrids was different between sites. At Transit Hill, we found no hybrid adult trees, but 13.5% of younger palms examined there were of late hybrid classes. In contrast, we found four hybrid adult trees, mostly of late hybrid classes, and only one juvenile F1 hybrid in the Far Flats. This pattern indicates that selection acts against hybrids between the juvenile and adult stages. An in situ reciprocal seed transplant between volcanic and calcareous soils also shows that early fitness components (up to 36 months) were affected by species and soil. These results are indicative of divergent selection in reproductive isolation, although it does not solely explain the current distribution of the two species on LHI

Reconstruction of Black Hole Metric Perturbations from Weyl Curvature

Perturbation theory of rotating black holes is usually described in terms of Weyl scalars $\psi_4$ and $\psi_0$, which each satisfy Teukolsky's complex master wave equation and respectively represent outgoing and ingoing radiation. On the other hand metric perturbations of a Kerr hole can be described in terms of (Hertz-like) potentials $\Psi$ in outgoing or ingoing {\it radiation gauges}. In this paper we relate these potentials to what one actually computes in perturbation theory, i.e $\psi_4$ and $\psi_0$. We explicitly construct these relations in the nonrotating limit, preparatory to devising a corresponding approach for building up the perturbed spacetime of a rotating black hole. We discuss the application of our procedure to second order perturbation theory and to the study of radiation reaction effects for a particle orbiting a massive black hole.Comment: 6 Pages, Revtex

Clinical features, antimicrobial susceptibility patterns and genomics of bacteria causing neonatal sepsis in a children's hospital in Vietnam: protocol for a prospective observational study.

INTRODUCTION: The clinical syndrome of neonatal sepsis, comprising signs of infection, septic shock and organ dysfunction in infants ≤4 weeks of age, is a frequent sequel to bloodstream infection and mandates urgent antimicrobial therapy. Bacterial characterisation and antimicrobial susceptibility testing is vital for ensuring appropriate therapy, as high rates of antimicrobial resistance (AMR), especially in low-income and middle-income countries, may adversely affect outcome. Ho Chi Minh City (HCMC) in Vietnam is a rapidly expanding city in Southeast Asia with a current population of almost 8 million. There are limited contemporary data on the causes of neonatal sepsis in Vietnam, and we hypothesise that the emergence of multidrug resistant bacteria is an increasing problem for the appropriate management of sepsis cases. In this study, we aim to investigate the major causes of neonatal sepsis and assess disease outcomes by clinical features, antimicrobial susceptibility profiles and genome composition. METHOD AND ANALYSIS: We will conduct a prospective observational study to characterise the clinical and microbiological features of neonatal sepsis in a major children's hospital in HCMC. All bacteria isolated from blood subjected to whole genome sequencing. We will compare clinical variables and outcomes between different bacterial species, genome composition and AMR gene content. AMR gene content will be assessed and stratified by species, years and contributing hospital departments. Genome sequences will be analysed to investigate phylogenetic relationships. ETHICS AND DISSEMINATION: The study will be conducted in accordance with the principles of the Declaration of Helsinki and the International Council on Harmonization Guidelines for Good Clinical Practice. Ethics approval has been provided by the Oxford Tropical Research Ethics Committee 35-16 and Vietnam Children's Hospital 1 Ethics Committee 73/GCN/BVND1. The findings will be disseminated at international conferences and peer-reviewed journals. TRIAL REGISTRATION NUMBER: ISRCTN69124914; Pre-results

Local Hidden Variables Underpinning of Entanglement and Teleportation

Entangled states whose Wigner functions are non-negative may be viewed as being accounted for by local hidden variables (LHV). Recently, there were studies of Bell's inequality violation (BIQV) for such states in conjunction with the well known theorem of Bell that precludes BIQV for theories that have LHV underpinning. We extend these studies to teleportation which is also based on entanglement. We investigate if, to what extent, and under what conditions may teleportation be accounted for via LHV theory. Our study allows us to expose the role of various quantum requirements. These are, e.g., the uncertainty relation among non-commuting operators, and the no-cloning theorem which forces the complete elimination of the teleported state at its initial port.Comment: 24 pages, 1 figure, accepted Found. Phy

Five-loop renormalization-group expansions for the three-dimensional n-vector cubic model and critical exponents for impure Ising systems

The renormalization-group (RG) functions for the three-dimensional n-vector cubic model are calculated in the five-loop approximation. High-precision numerical estimates for the asymptotic critical exponents of the three-dimensional impure Ising systems are extracted from the five-loop RG series by means of the Pade-Borel-Leroy resummation under n = 0. These exponents are found to be: \gamma = 1.325 +/- 0.003, \eta = 0.025 +/- 0.01, \nu = 0.671 +/- 0.005, \alpha = - 0.0125 +/- 0.008, \beta = 0.344 +/- 0.006. For the correction-to-scaling exponent, the less accurate estimate \omega = 0.32 +/- 0.06 is obtained.Comment: 11 pages, LaTeX, no figures, published versio

The CIPAZ study protocol: an open label randomised controlled trial of azithromycin versus ciprofloxacin for the treatment of children hospitalised with dysentery in Ho Chi Minh City, Vietnam

Background: Diarrhoeal disease remains a common cause of illness and death in children <5 years of age. Faecal-oral infection by Shigella spp. causing bacillary dysentery is a leading cause of moderate-to-severe diarrhoea, particularly in low and middle-income countries. In Southeast Asia, S. sonnei predominates and infections are frequently resistant to first-line treatment with the fluoroquinolone, ciprofloxacin. While resistance to all antimicrobials is increasing, there may be theoretical and clinical benefits to prioritizing treatment of bacillary dysentery with the azalide, azithromycin. In this study we aim to measure the efficacy of treatment with azithromycin compared with ciprofloxacin, the current standard of care, for the treatment of children with bacillary dysentery. Methods and analysis: We will perform a multicentre, open-label, randomized controlled trial of two therapeutic options for the antimicrobial treatment of children hospitalised with dysentery. Children (6–60 months of age) presenting with symptoms and signs of dysentery at Children’s Hospital 2 in Ho Chi Minh City will be randomised (1:1) to treatment with either oral ciprofloxacin (15mg/kg/twice daily for 3 days, standard-of-care) or oral azithromycin (10mg/kg/daily for 3 days). The primary endpoint will be the proportion of treatment failure (defined by clinical and microbiological parameters) by day 28 (+3 days) and will be compared between study arms by logistic regression modelling using treatment allocation as the main variable. Ethics and dissemination: The study protocol (version 1.2 dated 27th December 2018) has been approved by the Oxford Tropical Research Ethics Committee (47–18) and the ethical review boards of Children's Hospital 2 (1341/NĐ2-CĐT). The study has also been approved by the Vietnamese Ministry of Health (5044/QĐ-BYT). Trial registration: Clinicaltrials.gov: NCT03854929 (February 26th 2019)