Remodeling of Fibrous Extracellular Matrices by Contractile Cells: Predictions from Discrete Fiber Network Simulations

Contractile forces exerted on the surrounding extracellular matrix (ECM) lead to the alignment and stretching of constituent fibers within the vicinity of cells. As a consequence, the matrix reorganizes to form thick bundles of aligned fibers that enable force transmission over distances larger than the size of the cells. Contractile force-mediated remodeling of ECM fibers has bearing on a number of physiologic and pathophysiologic phenomena. In this work, we present a computational model to capture cell-mediated remodeling within fibrous matrices using finite element based discrete fiber network simulations. The model is shown to accurately capture collagen alignment, heterogeneous deformations, and long-range force transmission observed experimentally. The zone of mechanical influence surrounding a single contractile cell and the interaction between two cells are predicted from the strain-induced alignment of fibers. Through parametric studies, the effect of cell contractility and cell shape anisotropy on matrix remodeling and force transmission are quantified and summarized in a phase diagram. For highly contractile and elongated cells, we find a sensing distance that is ten times the cell size, in agreement with experimental observations.Comment: Accepted for publication in the Biophysical Journa

General Relativistic Simulations of Magnetized Plasmas around Merging Supermassive Black Holes

Coalescing supermassive black hole binaries are produced by the mergers of galaxies and are the most powerful sources of gravitational waves accessible to space-based gravitational observatories. Some such mergers may occur in the presence of matter and magnetic fields and hence generate an electromagnetic counterpart. In this Letter, we present the first general relativistic simulations of magnetized plasma around merging supermassive black holes using the general relativistic magnetohydrodynamic code Whisky. By considering different magnetic field strengths, going from non-magnetically dominated to magnetically dominated regimes, we explore how magnetic fields affect the dynamics of the plasma and the possible emission of electromagnetic signals. In particular we observe a total amplification of the magnetic field of ~2 orders of magnitude which is driven by the accretion onto the binary and that leads to much stronger electromagnetic signals, more than a factor of 10^4 larger than comparable calculations done in the force-free regime where such amplifications are not possible.Comment: 7 pages, 5 figures. Minor changes to match version accepted for publication on The Astrophysical Journal Letter

Tall Buildings and Their Foundations: Three Examples

The influence of foundations on the design and behavior of tall buildings is explored by examining two built towers: Burj Khalifa, Trump International Hotel and Tower, and the partially built Plaza Rakyat, a 77 story tower in Malaysia. The paper reviews how foundation conditions were considered in the design of the buildings, and how the foundations were anticipated to influence the behavior of the towers

Editorial: Multi-Omics Technologies for Optimizing Synthetic Biomanufacturing

No abstract availabl

Micropatterned Multicolor Dynamically Adhesive Substrates to Control Cell Adhesion and Multicellular Organization

We present a novel technique to examine cell–cell interactions and directed cell migration using micropatterned substrates of three distinct regions: an adhesive region, a nonadhesive region, and a dynamically adhesive region switched by addition of a soluble factor to the medium. Combining microcontact printing with avidin–biotin capture chemistry, we pattern nonadhesive regions of avidin that become adhesive through the capture of biotinylated fibronectin. Our strategy overcomes several limitations of current two-color dynamically adhesive substrates by incorporating a third, permanently nonadhesive region. Having three spatially and functionally distinct regions allows for the realization of more complex configurations of cellular cocultures as well as intricate interface geometries between two cell populations for diverse heterotypic cell–cell interaction studies. We can now achieve spatial control over the path and direction of migration in addition to temporal control of the onset of migration, enabling studies that better recapitulate coordinated multicellular migration and organization in vitro. We confirm that cellular behavior is unaltered on captured biotinylated fibronectin as compared to printed fibronectin by examining the cells’ ability to spread, form adhesions, and migrate. We demonstrate the versatility of this approach in studies of migration and cellular cocultures, and further highlight its utility by probing Notch–Delta juxtacrine signaling at a patterned interface

Bone, Biomarker, Body Composition, and Performance Responses to 8 Weeks of ROTC Training

Context: Military personnel engage in vigorous exercise, often resulting in higher bone mineral density; however, lower leg bone injuries are common in this population. Predictors of change in tibial bone quality and strength need to be characterized in this high-risk population. Objective: This study aimed to examine the effects of an eight-week military training intervention on total body and site-specific bone density and tibial bone quality, serum biomarkers (parathyroid hormone and sclerostin), body composition, and physical performance. Additionally, we sought to investigate what outcome variables (biomarkers, body composition, physical performance) would be predictive of estimated tibial bone strength in college-aged Reserve Officers\u27 Training Corps (ROTC) members. Design: Prospective Cohort Study. Setting: XXX University. Patients of Other Participants: ROTC (n=14 male; n=4 female) were matched for sex, age, and body mass to physically active Controls (n=14 male; n=4 female). ROTC engaged in an eight-week training intervention, while physically active Controls made no changes to their exercise routines. Main outcome measures: Pre general health questionnaires and pre, mid, and post intervention bone scans (DXA, pQCT), serum blood draws (parathyroid hormone and sclerostin), and physical performance measures (muscle strength and aerobic capacity) were tested. Results: ROTC participants exhibited significantly increased hip bone density and content (all p≤0.03) after the eight-week intervention. Sclerostin, not PTH, was a significant positive correlate and predictor in all ROTC models for estimated bone strength at the fracture prone 38% tibial site. Both groups decreased total body and regional fat mass and ROTC increased aerobic capacity (all p≤0.05). Conclusions: All bone, body composition, and performance measures either improved or were maintained in response to ROTC training and sclerostin should be further investigated as a potential early indicator of changes in estimated tibial bone strength in military cohorts

Nasal Congestion on the International Space Station

No abstract availabl

Origin of two-band chorus in the radiation belt of Earth.

Naturally occurring chorus emissions are a class of electromagnetic waves found in the space environments of the Earth and other magnetized planets. They play an essential role in accelerating high-energy electrons forming the hazardous radiation belt environment. Chorus typically occurs in two distinct frequency bands separated by a gap. The origin of this two-band structure remains a 50-year old question. Here we report, using NASA's Van Allen Probe measurements, that banded chorus waves are commonly accompanied by two separate anisotropic electron components. Using numerical simulations, we show that the initially excited single-band chorus waves alter the electron distribution immediately via Landau resonance, and suppress the electron anisotropy at medium energies. This naturally divides the electron anisotropy into a low and a high energy components which excite the upper-band and lower-band chorus waves, respectively. This mechanism may also apply to the generation of chorus waves in other magnetized planetary magnetospheres

Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning

Persistent alteration of protein conformation due to interaction with isoflurane may be a novel molecular aspect of preconditioning. We preincubated human serum albumin with isoflurane, dialyzed to release agent, and assessed protein conformation. Susceptibility to chemical modification by methylglyoxal and nitrophenylacetate was also examined. Isoflurane had a persistent effect on protein conformation. An increase in the susceptibility of surface residues to chemical modification attended this change in conformation. Modification of isoflurane-treated HSA included intra- and intersubunit cross-linking that may be a consequence of anesthetic-induced changes in multimeric subpopulations. This irreversible effect of isoflurane may represent a mechanism for preconditioning

Engineering robust polar chiral clathrate crystals

This is the author's accepted manuscript. The final published article is available from the link below. Copyright @ Royal Society of Chemistry 2013.The R-(+)-enantiomeric form of Dianin's compound and the S-(+)-enantiomeric form of its direct thiachroman analogue both obtained chromatographically employing a cellulose tris(3,5-dimethylphenylcarbamate) column, are shown to undergo supramolecular assembly to form a polar clathrate lattice which is stable even in the absence of a consolidating guest component