BioCultural Landscapes per la rigenerazione innovativa dei territori di montagna

Simplification of agricultural systems, farmland abandonment, uncontrolled urbanization, together with global scale drivers, determine fast and unpredictable phenomena of hydrogeological instability, biodiversity decline and identity loss. Men should hence promote socio-economic resilience and place identity, using biocultural landscapes as a resource. These landscapes are the outcome of the historical relationship between man and nature, resulting from complex interactions between biodiversity (at all levels, including species richness, ecosystem and biotope diversity) and cultural diversity, including material and immaterial aspects (architectural heritage, traditions, customs, local traditional agricultural practices, dialectal culture). Traditional water use is one of the main defining factors of traditional agricultural landscapes, characterized by significantly multiplied and enhanced functions and by the highest degree of biocultural diversity. The integrity of traditional agricultural landscapes is an indicator of environmental sustainability, socio-economic growth and habitat resilience. Land management and planning should promote the multi-functionality of biocultural landscapes through new interdisciplinary frameworks and innovative strategies, aimed at landscape conservation, enhancement and creative management

Ambienti umidi effimeri e naturalità del paesaggio in Sicilia

This paper analyzes the relationship between vegetal communities conservation state and surrounding landscape naturalness. Expert-based assessment, based on phytosociological releves, has been used to define the qualitative condition of vegetal coenoses. Landscape naturalness has been assessed using Naturalness Evaluation Index (NEI). Analysis showed strong correlation between landscape naturalness and assemblages conservation state. Ephemeral wetlands survival is tied to proper conservation of surrounding territory

Improvement of FAO-56 Model to Estimate Transpiration Fluxes of Drought Tolerant Crops under Soil Water Deficit: Application for Olive Groves

[EN] Agro-hydrological models are considered an economic and simple tool for quantifying crop water requirements. In the last two decades, agro-hydrological physically based models have been developed to simulate mass and energy exchange processes in the soil-plant-atmosphere system. Although very reliable, because of the high number of required variables, simplified models have been proposed to quantify crop water consumes. The main aim of this paper is to propose an amendment of the Food and Agricultural Organization (FAO) of the United Nations FAO-56 spreadsheet program to introduce a more realistic shape of the stress function, valid for mature olive orchards (Olea europaea L.). The modified model is successively validated by means of the comparison between measured and simulated soil water contents and actual transpiration fluxes. These outputs are finally compared with those obtained with the original version of the model. Experiments also allowed assessing the ability of simulated crop water stress coefficients to explain the actual water stress conditions evaluated on the basis of measured relative transpirations and midday stem water potentials. The results show that the modified model significantly improves the estimation of actual crop transpiration fluxes and soil water contents under soil water deficit conditions, according to the RMSEs associated with the revised model, resulting in significantly higher than the corresponding values obtained with the original version. (C) 2014 American Society of Civil Engineers.Rallo, G.; Baiamonte, G.; Manzano Juarez, J.; Provenzano, G. (2014). Improvement of FAO-56 Model to Estimate Transpiration Fluxes of Drought Tolerant Crops under Soil Water Deficit: Application for Olive Groves. Journal of Irrigation and Drainage Engineering. 140(9):1-8. doi:10.1061/(ASCE)IR.1943-4774.0000693S18140

Mapping floristic diversity: a case study in Sicily

This paper presents an operational methodology to map and analyze the floristic richness of “target species” in Natura 2000 sites, making use of G.I.S. tools and procedures. A Floristic diversity map (scale 1:50,000), covering an area of 612 km2, was produced by a team of experts as part of the management plans of “Madonie Mountains” Sites of Community Importance (SCIs), located in Sicily (Italy). The primary grid map represents the richness of “target species”, which include species of Community interests, taxa on the National Red List, endemic and threatened, species protected under International Conventions, taxa of phytogeographic importance. Secondary data frames include a three-dimensional map representing the number of species present in each cell, a coarser species richness distribution (scale 1:400,000) and a reference map of endemism rate in the Mediterranean area. Such a cartographic document has proven to be an effective tool in biodiversity conservation planning. Furthermore, the knowledge of floristic richness and distribution is not only important for the management of protected areas, but it is also important for the sustainable management of cultural landscapes

Multicavity halloysite-amphiphilic cyclodextrin hybrids for co-delivery of natural drugs into thyroid cancer cells

Multicavity halloysite nanotube materials were employed as simultaneous carriers for two different natural drugs, silibinin and quercetin, at 6.1% and 2.2% drug loadings, respectively. The materials were obtained by grafting functionalized amphiphilic cyclodextrin onto the HNT external surface. The new materials were characterized by FT-IR spectroscopy, SEM, thermogravimetry, turbidimetry, dynamic light scattering and ζ-potential techniques. The interaction of the two molecules with the carrier was studied by HPLC measurements and fluorescence spectroscopy, respectively. The release of the drugs from HNT-amphiphilic cyclodextrin, at two different pH values, was also investigated by means of UV-vis spectroscopy. Biological assays showed that the new complex exhibits anti-proliferative activity against human anaplastic thyroid cancer cell lines 8505C. Furthermore, fluorescence microscopy was used to evaluate whether the carrier was uptaken into 8505C thyroid cancer cell lines. The successful results revealed that the synthesized multicavity system is a material of suitable size to transport drugs into living cells

Endurance training: Is it bad for you?

Endurance exercise training exerts many positive effects on health, including improved metabolism, reduction of cardiovascular risk, and reduced all-cause and cardiovascular mortality. Intense endurance exercise causes mild epithelial injury and inflammation in the airways, but does not appear to exert detrimental effects on respiratory health or bronchial reactivity in recreational/ non-elite athletes. Conversely, elite athletes of both summer and winter sports show increased susceptibility to development of asthma, possibly related to environmental exposures to allergens or poor conditioning of inspired air, so that a distinct phenotype of “sports asthma” has been proposed to characterise such athletes, who more often practise aquatic and winter sports. Overall, endurance training is good for health but may become deleterious when performed at high intensity or volume

Correlation of tensile properties of Arc-Sprayed coatings and easy testing methods

Different techniques are usually employed to evaluate the mechanical properties of arc-sprayed coatings. In many situations, comparing properties is complex, and values extracted from tensile tests are required for structural projects. X6CrNi18-8 stainless steel and molybdenum were sprayed onto a mild steel substrate using the electric arc thermal spray technique to discuss this issue. After a detailed microstructure characterization, tensile tests were performed on both coatings to determine the yield strength and total elongation. Easy techniques were also applied: Vickers hardness and Charpy impact test. Tensile tests have shown that applying coatings increased the steel substrate’s total elongation. Molybdenum coating presented a higher impact resistance than the X6CrNi18-8 one, resulting in no correlation between elongation and Charpy values. On the other hand, correlations between hardness and yield strength were identified, opening a discussion on the effects of the microstructure and type of test used

Structure alteration of a sandy-clay soil by biochar amendments

The aim of the present study was to investigate structure alterations of a sandy-clay soil upon addition of different amounts of biochar (f (bc) ). All the f (bc) samples were analyzed by high energy moisture characteristic (HEMC) technique and H-1 nuclear magnetic resonance (NMR) relaxometry. HEMC was applied in order to evaluate aggregate stability of biochar-amended soil samples. H-1 NMR relaxometry experiments were conducted for the evaluation of the pore distributions through the investigation of water dynamics of the same samples. The HEMC technique revealed improvement in aggregate stability through measurements of the amount of drainable pores and the stability ratio. The latter increased as the amount of biochar was raised up. The H-1 NMR relaxometry revealed a unimodal T (1) distribution for both the sole sandy-clay soil and the biochar. Conversely, a bimodal T (1) distribution was acquired for all the different f (bc) samples. Improvement in aggregate stability was obtained as biochar was progressively added to the sandy-clay soil. A dual mechanism of water retention has been hypothesized. In particular, intra-aggregate porosity was indicated as the main responsible for molecular water diffusion when f (bc) comprised between 0 and 0.33. Conversely, inter-aggregate porosity resulted predominant, through swelling processes, when f (bc) overcame 0.33

Identification of Novel Wsf1 Mutations in a Sicilian Child with Wolfram Syndrome

Wolfram Syndrome (WS) is a rare hereditary disease with autosomal recessive inheritance with incomplete penetrance. It is characterized by diabetes mellitus associated with progressive optic atrophy. The diagnosis is essentially clinical and mutation analysis is used to confirm the diagnosis. In the present study we describe the clinical and molecular features of a diabetic child carrying two novel WFS1 mutations. The Sicilian proband and his non-affected family were studied. Ophthalmologic examination included: visual acuity determination and funduscopy, optical coherent tomography, retinal fluorangiography, perimetry and electroretinogram. Molecular methods: automatic sequencing of PCR amplified WFS1 gene fragments and qRT-PCR analysis of WFS1 transcripts. 3 WSF1 mutations have been identified in the proband. One allele carries 2 paternally inherited mutations (c.1332 C>G and c.1631C>G) in exon-8, never annotated before, in heterozygosis with one “de novo” classic mutation (c.505 G>A) in exon-5. In addition, we report an unexpected molecular feature: higher WFS1 mRNA levels in the proband compared to the father

Design and Synthesis of Novel Thieno[3,2-c]quinoline Compounds with Antiproliferative Activity on RET-Dependent Medullary Thyroid Cancer Cells

RET kinase gain-of-function mutations represent the main cause of the high aggressiveness and invasiveness of medullary thyroid cancer (MTC). The selective inhibition of the RET kinase is a suitable strategy for the treatment of this endocrine neoplasia. Herein, we performed an innovative ligand-based virtual screening protocol using the DRUDITonline web service, focusing on the RET kinase as a biological target. In this process, thieno[3,2-c]quinolines 6a-e and 7a-e were proposed as new potential RET inhibitors. The selected compounds were synthetized by appropriate synthetic strategies, and in vitro evaluation of antiproliferative properties conducted on the particularly aggressive MTC cell line TT(C634R) identified compounds 6a-d as promising anticancer agents, with IC50 values in the micromolar range. Further structure-based computational studies revealed a significant capability of the most active compounds to the complex RET tyrosine kinase domain. The interesting antiproliferative results supported by in silico predictions suggest that these compounds may represent a starting point for the development of a new series of small heterocyclic molecules for the treatment of MTC