60 research outputs found

    A theoretical model for template-free synthesis of long DNA sequence

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    This theoretical scheme is intended to formulate a potential method for high fidelity synthesis of Nucleic Acid molecules towards a few thousand bases using an enzyme system. Terminal Deoxyribonucleotidyl Transferase, which adds a nucleotide to the 3′OH end of a Nucleic Acid molecule, may be used in combination with a controlled method for nucleotide addition and degradation, to synthesize a predefined Nucleic Acid sequence. A pH control system is suggested to regulate the sequential activity switching of different enzymes in the synthetic scheme. Current practice of synthetic biology is cumbersome, expensive and often error prone owing to the dependence on the ligation of short oligonucleotides to fabricate functional genetic parts. The projected scheme is likely to render synthetic genomics appreciably convenient and economic by providing longer DNA molecules to start with

    A framework for priority-setting in climate smart agriculture research

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    Climate-smart agriculture (CSA) is widely promoted as an approach for reorienting agricultural development under the realities of climate change. Prioritising research-for-development activities is crucial, given the need to utilise scarce resources as effectively as possible. However, no framework exists for assessing and comparing different CSA research investments. Several aspects make it challenging to prioritise CSA research, including its multi-dimensional nature (productivity, adaptation and mitigation), the uncertainty surrounding many climate impacts, and the scale and temporal dependencies that may affect the benefits and costs of CSA adoption. Here we propose a framework for prioritising agricultural research investments across scales and review different approaches to setting priorities among agricultural research projects. Many priority-setting case studies address the short- to medium-term and at relatively local scales. We suggest that a mix of actions that span spatial and temporal time scales is needed to be adaptive to a changing climate, address immediate problems and create enabling conditions for enduring change

    Alupent® zur pharmakodynamischen Diagnostik kindlicher Herzfehler

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    Travel book: transportation international ...

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    Compilers: 1915-16, H. W. Phillips.--1917, O. Baedeker.--1918-24, H. W. Phillips.--1925- O. Baedeker and associates.Imprint varies: 1915-18, Washington, D.C., Independent printing companies of U.S.--1919-21, New York, H. W. Phillips,--1922-24, Baltimore, American travel & hotel directory co.--1925- Washington, D.C. [etc.] International transportation association.Mode of access: Internet.Vols. for include: Hotel book, and: Trade developer, also available separately
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