開拓使札幌本庁管内一覧概表 [書写資料] / 札幌本庁

統計 / 明治12年kosh

Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale.

Mass spectrometry (MS)-based ubiquitinomics provides system-level understanding of ubiquitin signaling. Here we present a scalable workflow for deep and precise in vivo ubiquitinome profiling, coupling an improved sample preparation protocol with data-independent acquisition (DIA)-MS and neural network-based data processing specifically optimized for ubiquitinomics. Compared to data-dependent acquisition (DDA), our method more than triples identification numbers to 70,000 ubiquitinated peptides in single MS runs, while significantly improving robustness and quantification precision. Upon inhibition of the oncology target USP7, we simultaneously record ubiquitination and consequent changes in abundance of more than 8,000 proteins at high temporal resolution. While ubiquitination of hundreds of proteins increases within minutes of USP7 inhibition, we find that only a small fraction of those are ever degraded, thereby dissecting the scope of USP7 action. Our method enables rapid mode-of-action profiling of candidate drugs targeting DUBs or ubiquitin ligases at high precision and throughput

Evaluation of secure authentication products in a Windows environment

In this report hardware based alternatives to password authentication in a Windows domain are evaluated for the needs of a certain company. In order to investigate the demands on such alternatives interviews with people concerned have been carried out. The demands which resulted from the interviews have been used to select types of hardware tokens for evaluation. Two products which offer authentication with smart cards and USB tokens have been selected and closer evaluated. These are RSA Passage which offers both hardware options and Rainbow iKey which uses USB tokens. Both products are evaluated based on the demands and additional evaluation criteria. The information from the evaluations is used to compare the products

Tjänstekvalitet inom bankbranschen : En studie om företagskunders förväntningar och upplevelser i en föränderlig marknad

Banker har i stor utsträckning övergått från traditionella interpersonella leveranser till digitala alternativ, genom användandet av självbetjäningsteknik (SST) (Johns & Perrot, 2008). Detta har medfört minskning av antalet bankkontor, framförallt i Norden (Dallerup et al. 2018; Svenska Bankföreningen, 2020). Samtidigt visar forskning att företagskunder önskar en nära och personlig relation vid anskaffandet av komplexa tjänster (Chalmers & Stubs 2019). Studien adresserade därför detta genom att öka kunskapen om företagskunders uppfattning av tjänstekvalitet vid nyttjandet av banktjänster och interaktioner med deras bank genom följande forskningsfrågor: FF1: Hur kan företagskunders förväntningar på banktjänster beskrivas? FF2: Hur kan företagskunders upplevelser av banktjänster beskrivas? Metoden som nyttjades för detta var en deduktiv kvalitativ ansats med kvantitativa inslag, där semi-strukturerade intervjuer genomfördes baserade på SERVQUAL (Parasuraman et al., 1985) och SSTQUAL (Radomir och Nistor, 2012) kvalitetsdimensioner, följt av en tematisk analys. Studiens resultat pekade på följande faktorer som viktiga för hög tjänstekvalitet i banksektorn: God, tillitsfull och personlig relation Tillgänglighet och snabba beslut/handläggning Lokal närvaro/kontor Vidare sågs digital självbetjäningsteknik (SST) som ett fundamentalt behov hos företagskunderna och påverkade upplevd tjänstekvalitet vid banktjänster i liten utsträckning. Konklusionen är därför att det idag är svårt att fullt ut ersätta ansikte-mot-ansikte interaktioner med digitala SST-tjänster för företagskunder. Vid de tillfällen lokala bankkontor stängts ned uppfattade samtliga av studiens respondenter det som negativt och har som en följd antingen bytt bank eller funderar på att göra det. Banker rekommenderas att anpassa sin verksamhetsstyrning i linje med de viktigaste faktorerna för upplevd tjänstekvalitet hos företagskunder.  Framtida studier rekommenderas att undersöka; (1) Om väsentlig ökad utbredning av videomöten under 2020-2021 över tid kan ersätta behovet för lokal förankring och fysiska möten, (2) Huruvida bankers leveransmodell mot företagskunder behöver anpassas och bli mer proaktiv, för att överbrygga de få interaktioner som finns i dagens digitala värld. Detta för att bygga upp de nödvändiga relationerna och tilliten som värdesätts så högt. Banks has largely shifted from interpersonal service deliveries to digital alternatives through the  use of self-service-technologies (SST) (Johns and Perrot, 2008). This has led to a reduction of bank branches, especially in the Nordic countries (Dallerup et al. 2018; Svenska Bankföreningen, 2020). Simultaneously research shows that banks B2B-customers wants a close and personal relationship as well as interpersonal meetings, especially in the procurement of complex services (Chalmers & Stubs 2019). The study addressed this by increasing the knowledge of B2B-customers perceptions of service quality when using bank services and interactions with their bank through the following research questions:  RQ1: How can B2B-customers expectations of banking services be described? RQ2: How can B2B-customers perceptions of banking services be described?  The method used was a deductive qualitative approach with quantitative elements. Semi-structured interviews based on the quality dimensions of SERVQUAL (Parasuraman et al., 1985) and SSTQUAL (Radomir and Nistor, 2012) were conducted, followed by a thematic analysis. The study’s results deemed the following factors as important for high service quality in the banking sector:  Good, trusting, and personal relationship Availability and quick decisions/processing Local presence/branches  Furthermore, digital self-service-technologies (SST) was seen as a fundamental need among the B2B-customers and affected their perceptions of service quality in banking services to a small extent. The conclusion is that its difficult to fully replace face-to-face interactions with digital SST services for B2B-customers. At the times when local bank branches were closed, the study’s respondents perceived it as negative, and as a result they have either changed their bank or were considering doing so. It is recommended for banks to adapt their managerial strategy in line with the most important factors for perceived service quality among B2B-customers. Future studies are recommended to investigate the following: (1) If the significant increase of digital meetings during 2020-2021 over time can replace the need for local presence and physical meetings, (2) If banks delivery model towards B2B-customers needs to become more proactive to increase the number of interactions between the bank and its B2B-customers. To enhance the necessary relationships and trust that they highly value

Colonization of patients, healthcare workers, and the environment with healthcare-associated Staphylococcus epidermidis genotypes in an intensive care unit : a prospective observational cohort study

Background: During the last decades, healthcare-associated genotypes of methicillin-resistant Staphylococcus epidermidis (HA-MRSE) have been established as important opportunistic pathogens. However, data on potential reservoirs on HA-MRSE is limited. The aim of the present study was to investigate the dynamics and to which extent HA-MRSE genotypes colonize patients, healthcare workers (HCWs) and the environment in an intensive care unit (ICU). Methods: Over 12 months in 2006-2007, swab samples were obtained from patients admitted directly from the community to the ICU and patients transferred from a referral hospital, as well as from HCWs, and the ICU environment. Patients were sampled every third day during hospitalization. Antibiotic susceptibility testing was performed according to EUCAST guidelines. Pulsed-field gel electrophoresis and multilocus sequence typing were used to determine the genetic relatedness of a subset of MRSE isolates. Results: We identified 620 MRSE isolates from 570 cultures obtained from 37 HCWs, 14 patients, and 14 environmental surfaces in the ICU. HA-MRSE genotypes were identified at admission in only one of the nine patients admitted directly from the community, of which the majority subsequently were colonized by HA-MRSE genotypes within 3 days during hospitalization. Almost all (89%) of HCWs were nasal carriers of HA-MRSE genotypes. Similarly, a significant proportion of patients transferred from the referral hospital and fomites in the ICU were widely colonized with HA-MRSE genotypes. Conclusions: Patients transferred from a referral hospital, HCWs, and the hospital environment serve as important reservoirs for HA-MRSE. These observations highlight the need for implementation of effective infection prevention and control measures aiming at reducing HA-MRSE transmission in the healthcare setting

A dynamic gradient of Wnt signaling controls initiation of neurogenesis in the mammalian cortex and cellular specification in the hippocampus

AbstractNeurogenesis in the developing neocortex is a strictly regulated process of cell division and differentiation. Here we report that a gradual retreat of canonical Wnt signaling in the cortex from lateral-to-medial and anterior-to-posterior is a prerequisite of neurogenesis. Ectopic expression of a β-catenin/LEF1 fusion protein maintains active canonical Wnt signaling in the developing cortex and delays the expression onset of the neurogenic factors Pax6, Ngn2 and Tbr2 and subsequent neurogenesis. Contrary to this, conditional ablation of β-catenin accelerates expression of the same neurogenic genes. Furthermore, we show that a sustained canonical Wnt activity in the lateral cortex gives rise to cells with hippocampal characteristics in the cortical plate at the expense of the cortical fate, and to cells with dentate gyrus characteristics in the hippocampus. This suggests that the dose of canonical Wnt signaling determines cellular fate in the developing cortex and hippocampus, and that recession of Wnt signaling acts as a morphogenetic gradient regulating neurogenesis in the cortex

El Diario de Pontevedra : periódico liberal: Ano XXVI Número 7469 - 1909 abril 3

The dynamics of the Mig1-quotient. (PDF 464 kb

Additional file 9: Table S2. of Single-cell study links metabolism with nutrient signaling and reveals sources of variability

S. cerevisiae strains used in this study. (PDF 26 kb