CIVIC EPISTEMOLOGIES – Development of a Roadmap for Citizen Researchers in the Age of Digital Culture

The CIVIC EPISTEMOLOGIES project investigates citizen science and crowdsourcing in the domain of the research in Digital Cultural Heritage and Humanities (DCHH). The ultimate aim is to produce a validated Roadmap indicating the suggested direction that the deployment of services and infrastructures should take, in order to support the participation of citizens in the research processes and the participation of creative industries in the exploitation of digital cultural content. The case of DCHH is particularly relevant because of the major cross-cutting role that the humanities play in European research and innovation, recently acknowledged in a clear way in the Horizon2020 Community Programme for Research and Innovation. Cultural heritage and humanities also represent a subject area in which citizens are particularly active, counting several – still spread - experiences of their involvement in recording, annotating and cataloguing activities on an individual or group basis, as volunteers and amateurs. The case of broadening e-Infrastructure deployment to support the participation of citizens to DCHH research, even if holding a strong impact potential for social cohesion and job development, is not yet fully explored. The paper discusses about the multidisciplinary approach to citizen science and how this method can contribute to the benefit of many scientific domains, research communities, and technology advancements as well as delivering novel social and economic impact

A common core RNP structure shared between the small nuclear box C/D RNPs and the spliceosomal U 4 snRNP.

AbstractThe box C/D snoRNAs function in directing 2′-O-methylation and/or as chaperones in the processing of ribosomal RNA. We show here that Snu13p (15.5kD in human), a component of the U4/U6.U5 tri-snRNP, is also associated with the box C/D snoRNAs. Indeed, genetic depletion of Snu13p in yeast leads to a major defect in RNA metabolism. The box C/D motif can be folded into a stem-internal loop-stem structure, almost identical to the 15.5kD binding site in the U4 snRNA. Consistent with this, the box C/D motif binds Snu13p/15.5kD in vitro. The similarities in structure and function observed between the U4 snRNP (chaperone for U6) and the box C/D snoRNPs raises the interesting possibility that these particles may have evolved from a common ancestral RNP

The Localization of ss-Wave and Quantum Effective Potential of a Quasi-Free Particle with Position-Dependent Mass

The properties of the s-wave for a quasi-free particle with position-dependent mass(PDM) have been discussed in details. Differed from the system with constant mass in which the localization of the s-wave for the free quantum particle around the origin only occurs in two dimensions, the quasi-free particle with PDM can experience attractive forces in $D$ dimensions except D=1 when its mass function satisfies some conditions. The effective mass of a particle varying with its position can induce effective interaction which may be attractive in some cases. The analytical expressions of the eigenfunctions and the corresponding probability densities for the s-waves of the two- and three-dimensional systems with a special PDM are given, and the existences of localization around the origin for these systems are shown.Comment: 12pages, 8 figure

Tumors escape immunosurveillance by overexpressing the proteasome activator PSME3

The success of CD8+ T cell-based cancer immunotherapy emphasizes the importance of understanding the mechanisms of generation of MHC-I peptide ligands and the possible pathways of tumor cell escape from immunosurveillance. Recently, we showed that peptides generated in the nucleus during a pioneer round of mRNA translation (pioneer translation products, or PTPs) are an important source of tumor specific peptides which correlates with the aberrant splicing and transcription events associated with oncogenesis. Here we show that up-regulation of PSME3 proteasome activator in cancer cells results in increased destruction of PTP-derived peptides in the nucleus thus enabling cancer cell to subvert immunosurveillance. These findings unveil a previously unexpected role for PSME3 in antigen processing and identify PSME3 as a druggable target to improve the efficacy of cancer immunotherapy

Protein fingerprints of cultured CA3-CA1 hippocampal neurons: comparative analysis of the distribution of synaptosomal and cytosolic proteins

<p>Abstract</p> <p>Background</p> <p>All studies aimed at understanding complex molecular changes occurring at synapses face the problem of how a complete view of the synaptic proteome and of its changes can be efficiently met. This is highly desirable when synaptic plasticity processes are analyzed since the structure and the biochemistry of neurons and synapses get completely reshaped. Because most molecular studies of synapses are nowadays mainly or at least in part based on protein extracts from neuronal cultures, this is not a feasible option: these simplified versions of the brain tissue on one hand provide an homogeneous pure population of neurons but on the other yield only tiny amounts of proteins, many orders of magnitude smaller than conventional brain tissue. As a way to overcome this limitation and to find a simple way to screen for protein changes at cultured synapses, we have produced and characterized two dimensional electrophoresis (2DE) maps of the synaptic proteome of CA3-CA1 hippocampal neurons in culture.</p> <p>Results</p> <p>To obtain 2D maps, hippocampal cultures were mass produced and after synaptic maturation, proteins were extracted following subfractionation procedures and separated by 2D gel electrophoresis. Similar maps were obtained for the crude cytosol of cultured neurons and for synaptosomes purified from CA3-CA1 hippocampal tissue. To efficiently compare these different maps some clearly identifiable reference points were molecularly identified by mass spectrometry and immunolabeling methods. This information was used to run a differential analysis and establish homologies and dissimilarities in these 2D protein profiles.</p> <p>Conclusion</p> <p>Because reproducible fingerprints of cultured synapses were clearly obtained, we believe that our mapping effort could represent a simple tool to screen for protein expression and/or protein localization changes in CA3-CA1 hippocampal neurons following plasticity.</p

Medidas lineares preliminares de cascos de diferentes categorias de cavalos pantaneiros premiadas em pista de julgamento.

Os cascos constituem a base de sustentação de qualquer raça de cavalos, portanto, medidas biométricas dos cascos para as diferentes raças são registros importantes como referência e futuras avaliações quantitativas do equilíbrio do casco. Este estudo teve como objetivo avaliar cavalos Pantaneiros de diferentes categorias premiados em pistas de julgamento da raça em Corumbá, MS, em setembro de 2010. The hooves are the sustentation base of any horse breed, thus biometric measures for the different breeds are important records for reference and future quantitative assessments of the hoof balance. This study aimed to evaluate different categories of winners horses in judging in Corumbá city, in September 2010

Critérios visuais subjetivos na avaliação da performance funcional de cavalos Pantaneiros durante julgamento.

A obtenção de critérios (escores) subjetivos confiáveis na avaliação do desempenhofuncional dos cavalos Pantaneiros pode auxiliar os juízes e também os criadores na seleção e treinamento da raça. Este estudo teve como objetivo avaliar o uso de escores subjetivos na avaliação de cavalos Pantaneiros, durante julgamento realizado em Corumbá, MS, em setembro de 2010. Reliable subjective criterions (scores) for assessing functional performance of the Pantaneiros horses can help judges and breeders in selecting and training the breed. This work aimed to evaluate the use of subjective scores (classifiers) in the evaluation of adult Pantaneiros horses during Judgment in Corumbá, MS, in September 2010

Effect of a 40-weeks multicomponent exercise program and branched chain amino acids supplementation on functional fitness and mental health in frail older persons

BACKGROUND: The ageing process implies several physiological and psychological changes that hence affect the general health, mood states, and quality of life of older persons. Exercise and adequate nutrition are renowned non-pharmacological strategies that significantly delay and alleviate the adverse consequences of the ageing process. This study aimed to evaluate the effects of branched-chain amino acid (BCAA) supplementation and a multicomponent exercise program (ME) on the physical frailty and mood states of older persons. METHODS: 35 participants (women and men; 83&nbsp;±&nbsp;3&nbsp;years old) from residential care homes were submitted to a 40-week exercise-washout-retraining intervention (16&nbsp;weeks of the elastic band based exercise and/or supplementation, 8&nbsp;weeks of washout, and 16&nbsp;weeks of multicomponent exercise and/or resupplementing), with or without BCAA supplementation. The experimental groups were: (i) ME plus BCAA supplementation (ME+BCAA); (ii) ME; (iii) BCAA supplementation (BCAA), and (iv) control group (CG). Fried's phenotype was used to assess frailty prevalence. Geriatric Depression Scale (GDS), Profile of Mood State (POMS), Mini-Mental State Examination (MMSE), were used to access mental health and cognition. The Short Physical Performance Battery (SPPB) was used to access functional capacity. Salivary testosterone levels (ST) were also determined to access the anabolic effects of the intervention. RESULTS: Exercise was effective in improving functional capacity and prevented the increase in frailty that occurred in the non-exercising CG, where the frailty scores increased over time (p&nbsp;&lt;&nbsp;0.01). BCAAs supplement alone had no impact on functional fitness, but in a short time (16&nbsp;weeks) contributed to diminishing frailty and combined with exercise may have the potential to reduce the effect of a detraining period on functional capacity. Salivary testosterone levels correlated with handgrip strength and could be a useful indicator of susceptibility to frailty. No effects were found for mood states, cognition, and depression. CONCLUSION: This study showed that a long-term exercise program, independent of being multicomponent or strength elastic band-based, was effective in improving functional capacity and prevented an increase in frailty in frail and pre-frail older persons living in residential care homes

IRSp53 controls plasma membrane shape and polarized transport at the nascent lumen in epithelial tubules

It is unclear whether the establishment of apical–basal cell polarity during the generation of epithelial lumens requires molecules acting at the plasma membrane/actin interface. Here, we show that the I-BAR-containing IRSp53 protein controls lumen formation and the positioning of the polarity determinants aPKC and podocalyxin. Molecularly, IRSp53 acts by regulating the localization and activity of the small GTPase RAB35, and by interacting with the actin capping protein EPS8. Using correlative light and electron microscopy, we further show that IRSp53 ensures the shape and continuity of the opposing plasma membrane of two daughter cells, leading to the formation of a single apical lumen. Genetic removal of IRSp53 results in abnormal renal tubulogenesis, with altered tubular polarity and architectural organization. Thus, IRSp53 acts as a membrane curvature-sensing platform for the assembly of multi-protein complexes that control the trafficking of apical determinants and the integrity of the luminal plasma membrane