Caprine arthritis encephalitis virus disease modelling review

Mathematical modelling is used in disease studies to assess the economical impacts of diseases, as well as to better understand the epidemiological dynamics of the biological and environmental factors that are associated with disease spreading. For an incurable disease such as Caprine Arthritis Encephalitis (CAE), this knowledge is extremely valuable. However, the application of modelling techniques to CAE disease studies has not been significantly explored in the literature. The purpose of the present work was to review the published studies, highlighting their scope, strengths and limitations, as well to provide ideas for future modelling approaches for studying CAE disease. The reviewed studies were divided into the following two major themes: Mathematical epidemiological modelling and statistical modelling. Regarding the epidemiological modelling studies, two groups of models have been addressed in the literature: With and without the sexual transmission component. Regarding the statistical modelling studies, the reviewed articles varied on modelling assumptions and goals. These studies modelled the dairy production, the CAE risk factors and the hypothesis of CAE being a risk factor for other diseases. Finally, the present work concludes with further suggestions for modelling studies on CAE

Crosstalk between MLO-Y4 osteocytes and C2C12 muscle cells is mediated by the Wnt/β-catenin pathway

We examined the effects of osteocyte secreted factors on myogenesis and muscle function. MLO-Y4 osteocyte-like cell conditioned media (CM) (10%) increased ex vivo soleus muscle contractile force by ∼25%. MLO-Y4 and primary osteocyte CM (1-10%) stimulated myogenic differentiation of C2C12 myoblasts, but 10% osteoblast CMs did not enhance C2C12 cell differentiation. Since WNT3a and WNT1 are secreted by osteocytes, and the expression level of Wnt3a is increased in MLO-Y4 cells by fluid flow shear stress, both were compared, showing WNT3a more potent than WNT1 in inducing myogenesis. Treatment of C2C12 myoblasts with WNT3a at concentrations as low as 0.5ng/mL mirrored the effects of both primary osteocyte and MLO-Y4 CM by inducing nuclear translocation of β-catenin with myogenic differentiation, suggesting that Wnts might be potential factors secreted by osteocytes that signal to muscle cells. Knocking down Wnt3a in MLO-Y4 osteocytes inhibited the effect of CM on C2C12 myogenic differentiation. Sclerostin (100ng/mL) inhibited both the effects of MLO-Y4 CM and WNT3a on C2C12 cell differentiation. RT-PCR array results supported the activation of the Wnt/β-catenin pathway by MLO-Y4 CM and WNT3a. These results were confirmed by qPCR showing up-regulation of myogenic markers and two Wnt/β-catenin downstream genes, Numb and Flh1. We postulated that MLO-Y4 CM/WNT3a could modulate intracellular calcium homeostasis as the trigger mechanism for the enhanced myogenesis and contractile force. MLO-Y4 CM and WNT3a increased caffeine-induced Ca2+ release from the sarcoplasmic reticulum (SR) of C2C12 myotubes and the expression of genes directly associated with intracellular Ca2+ signaling and homeostasis. Together, these data show that in vitro and ex vivo, osteocytes can stimulate myogenesis and enhance muscle contractile function and suggest that Wnts could be mediators of bone to muscle signaling, likely via modulation of intracellular Ca2+ signaling and the Wnt/ β-Catenin pathway

Sarcopenia: pharmacology of today and tomorrow

ABSTRACT Sarcopenia remains largely undiagnosed and undertreated because of the lack of a universally accepted definition, effective ways to measure it, and identification of the outcomes that should guide treatment efficacy. An ever-growing number of clinicians and researchers along with funding and regulatory agencies have gradually recognized that sarcopenia is a human condition that requires both prevention and treatment. In this article, we review sarcopenia and its common and less known pharmacological treatments, attempt to define sarcopenia in its broader context, and present some new ideas for potential future treatment for this devastating condition

Certifying forest-based ecosystem services: results from the world-first application of the FSC ecosystem service procedure in Italy

In recent years, several mechanisms have been put in practice to value forest-based ecosystem services in order to maintain or enhance their provision. Mechanisms range from more traditional - e.g. regulation of land use, taxes and subsidies - to more innovative market-based ones such as Payments for Ecosystem Services (PES). More recently, the use of voluntary third-party forest certification schemes based on standards and procedures specifically aimed at considering the provision of ecosystem services has gained interests. Certification schemes have already been established for specific ecosystem services, in particular focusing on carbon, in some cases including biodiversity conservation as a co-benefit. The Forest Stewardship Council\uae (FSC) is currently the only scheme promoting the certification of multiple ecosystem services by demonstrating the positive impacts of FSC-certified forest management practices on five regulating and cultural services. A dedicated procedure has been developed, that allows the assessment of the impact of forest management on carbon sequestration and storage, biodiversity conservation, watershed services, soil conservation and recreational services, by means of specific methodologies and indicators. We critically analyzed the world-first application of the FSC ecosystem services certification procedure with reference to the forest certification group Waldplus and Associazione Forestale di Pianura (Lowland Forest Association) in northern Italy. The case study includes 1,043 hectares of different forest types, from semi-natural productive forests to conservation forests, traditionally managed to deliver multiple benefits. Our objective is to give insights that could inform and improve future developments in the field of certification, promotion and marketing of ecosystem services

Instruments politiques pour la préservation ou la restauration des espaces boisés et l’amélioration de la fourniture de biens et services forestiers

Dans la région méditerranéenne, les décideurs et les gestionnaires d’espaces forestiers cherchent des instruments politiques afin de combler l’écart entre la rentabilité financière et la rentabilité économique dans la gestion forestière. L’article passe en revue ces instruments politiques en se concentrant sur les instruments fondés sur le marché et plus particulièrement sur les paiements pour services environnementaux (PSE). Il est question notamment des problèmes de mise en œuvre des systèmes de PSE, en reconsidérant les obstacles et les opportunités offertes à leur développement dans la région méditerranéenne

Policy instruments to preserve or restore woodlands and to improve the supply of forest goods and services

In the Mediterranean region, policy makers and forest managers are seeking for policy instruments to fill the gap between financial and economic profitability in forest management. This paper reviews these policy instruments focusing on the market-based instruments and specifically on payments for environmental service (PES). We focus on the implementation problems of PES schemes looking at barriers and opportunities offered to their development in the Mediterranean region

Fibroblast Growth Factor 23 Does Not Directly Influence Skeletal Muscle Cell Proliferation and Differentiation or Ex Vivo Muscle Contractility

Skeletal muscle dysfunction accompanies the clinical disorders of chronic kidney disease (CKD) and hereditary hypophosphatemic rickets. In both disorders, fibroblast growth factor 23 (FGF23), a bone-derived hormone regulating phosphate and vitamin D metabolism, becomes chronically elevated. FGF23 has been shown to play a direct role in cardiac muscle dysfunction; however, it is unknown whether FGF23 signaling can also directly induce skeletal muscle dysfunction. We found expression of potential FGF23 receptors ( Fgfr1-4) and α-Klotho in muscles of two animal models (CD-1 and Cy/+ rat, a naturally occurring rat model of chronic kidney disease-mineral bone disorder) as well as C2C12 myoblasts and myotubes. C2C12 proliferation, myogenic gene expression, oxidative stress marker 8-OHdG, intracellular Ca2+ ([Ca2+]i), and ex vivo contractility of extensor digitorum longus (EDL) or soleus muscles were assessed after treatment with various amounts of FGF23. FGF23 (2-100 ng/ml) did not alter C2C12 proliferation, expression of myogenic genes, or oxidative stress after 24- to 72-h treatment. Acute or prolonged FGF23 treatment up to 6 days did not alter C2C12 [Ca2+]i handling, nor did acute treatment with FGF23 (9-100 ng/ml) affect EDL and soleus muscle contractility. In conclusion, although skeletal muscles express the receptors involved in FGF23-mediated signaling, in vitro FGF23 treatments failed to directly alter skeletal muscle development or function under the conditions tested. We hypothesize that other endogenous substances may be required to act in concert with FGF23 or apart from FGF23 to promote muscle dysfunction in hereditary hypophosphatemic rickets and CKD

Sindbad: A new operational service for a safer leisure and boating navigation

The SINDBAD- Leisure and Boating Safety Navigation - project goal is the development of an advanced operational service to support navigation in a specific area. The first prototype covers the Ligurian Sea (a very busy touristic area in the North Mediterranean Sea) It develops an ICT Service Infrastructure to provide innovative intelligent automation functions and to develop customized services, accessible by your mobile device, for conducting a boat and avoiding any kind of risk ensuring the best degree of comfort

Biomedical Tissue Engineering - Where We Go in the Future

Researchers and entrepreneurs examine breakthroughs in tissue engineering and regeneration that enhance healing, remodeling, and recovery