Phosphoric Acid Increases the Porosity and Extends the Contact Area of Dental Osseo integrated Implants

The surface treatments are performed in dental implants in order to increase the chemical and mechanical connection between the implant and bone, favoring the stability of implant-supported prostheses. The aim of this study was to characterize dental implant surfaces treated with 37% phosphoric acid. Implant surfaces were evaluated divided into groups of fifty samples being distributed in: porcelain samples without treatment; metal samples without treatment; porcelain samples with treatment with 37% phosphoric acid for 30 seconds; metal samples treatment with phosphoric acid at 37% for 30 seconds; porcelain samples with treatment with 37% phosphoric acid for 60 seconds; metal samples with treatment with 37% phosphoric acid for 60 seconds. The samples were characterized by Scanning Electron Microscopy. After the phosphoric acid treatment porosity changes were observed and expanding the contact area. The results show benefits of using phosphoric acid, as a surface with increased roughness; this is desired to occur matrix deposition and growth of bone tissue and facilitates the fixation of implant-supported prostheses

Caracterização socioeconômica das comunidades Chané/Bonfim, sub-região do Paraguai, Corumbá, MS.

Esse estudo caracteriza-se por ser um zoneamento das comunidades ribeirinhas da sub-região do Paraguai.bitstream/CPAP-2009-09/56843/1/CT82.pd

Produtividade de clones da variedade vitoria sob sistema de cultivo sequeiro no vale de Jequitinhonha-MG.

A variedade vitória que se caracteriza pela tolerância à seca, alta adaptabilidade e estabilidade de produção, surge como uma alternativa ao cultivo sequeiro na região do Vale do Jequitinhonha-MG. Entretanto, como os clones possuem heterogeneidade genética pode haver variação na sua capacidade de aclimatação. Assim, esse trabalho objetivou avaliar a produtividade inicial de clones da variedade vitória sob cultivo sequeiro no vale do Jequitinhonha. Para tanto, foram implantados 13 clones da variedade Vitória no delineamento foi blocos casualizados, com quatro repetições e parcelas constituídas por seis plantas e espaçamento de 3,0 x 1,0 m. A média de produtividade de todos os clones que compõem a variedade no biênio 2011/2012 foi de 51,53 sc.benef./há, sendo que a segunda colheita apresentou produtividade média 85% maior em relação a primeira. Os clones que mais se destacaram foram os 7V, 9V e 10V, com produtividades médias do primeiro biênio oscilando entre 69,53 a 82,11 sc. benef./ha. Houve variabilidade genética e ambiental entre os clones quanto às características relação coco/beneficiado, percentagem de frutos chochos, percentagem de grãos com peneira média superior a 13 e percentagem de grãos tipo moca, porém a maioria dos clones apresentaram esses parâmetros dentro de limites aceitáveis para a variedade Vitória. De maneira geral, a variedade Vitória possui elevada capacidade produtiva inicial nas condições no Vale do Jequitinhonha

Elevated alpha-synuclein caused by SNCA gene triplication impairs neuronal differentiation and maturation in Parkinson's patient-derived induced pluripotent stem cells

We have assessed the impact of α-synuclein overexpression on the differentiation potential and phenotypic signatures of two neural-committed induced pluripotent stem cell lines derived from a Parkinson´s disease patient with a triplication of the human SNCA genomic locus. In parallel, comparative studies were performed on two control lines derived from healthy individuals and lines generated from the patient iPS-derived neuroprogenitor lines infected with a lentivirus incorporating a small hairpin RNA to knock down the SNCA mRNA. The SNCA triplication lines exhibited a reduced capacity to differentiate into dopaminergic or GABAergic neurons and decreased neurite outgrowth and lower neuronal activity compared with control cultures. This delayed maturation phenotype was confirmed by gene expression profiling, which revealed a significant reduction in mRNA for genes implicated in neuronal differentiation such as delta-like homolog 1 (DLK1), gamma-aminobutyric acid type B receptor subunit 2 (GABABR2), nuclear receptor related 1 protein (NURR1), G-protein-regulated inward-rectifier potassium channel 2 (GIRK-2) and tyrosine hydroxylase (TH). The differentiated patient cells also demonstrated increased autophagic flux when stressed with chloroquine. We conclude that a two-fold overexpression of α-synuclein caused by a triplication of the SNCA gene is sufficient to impair the differentiation of neuronal progenitor cells, a finding with implications for adult neurogenesis and Parkinson´s disease progression, particularly in the context of bioenergetic dysfunction.Fil: Oliveira, L. M. A.. Max-Planck-Institut für biophysikalische Chemie; AlemaniaFil: Falomir Lockhart, Lisandro Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina. Max-Planck-Institut für biophysikalische Chemie; AlemaniaFil: Botelho, M. G.. Max-Planck-Institut für biophysikalische Chemie; Alemania. Universidade Federal do Rio de Janeiro; BrasilFil: Lin, K. H.. Max-Planck-Institut für biophysikalische Chemie; AlemaniaFil: Wales, P.. Universität Göttingen; AlemaniaFil: Koch, J. C.. Universität Göttingen; AlemaniaFil: Gerhardt, Elizabeth. Universität Göttingen; AlemaniaFil: Taschenberger, H.. Max-Planck-Institut für biophysikalische Chemie; AlemaniaFil: Outeiro, T. F.. Universität Göttingen; AlemaniaFil: Lingor, P.. Universität Göttingen; AlemaniaFil: Schüele, B.. The Parkinson’s Institute; Estados UnidosFil: Arndt Jovin, D. J.. Max-Planck-Institut für biophysikalische Chemie; AlemaniaFil: Jovin, T. M.. Max-Planck-Institut für biophysikalische Chemie; Alemani