Recurrent selection of pumpkin (Cucurbita moschata) genotypes to increase fruit quality.

The aim of pumpkin breeding programs is to develop cultivars that meet the production, commercial quality, and nutritional value, considering several attributes. Therefore, the objective of this study was to select pumpkin genotypes with high fruit quality for future use in genetic breeding programs. Two production cycles were carried out. The evaluations included the determination of genetic parameters (the additive genetic variance, environmental variance between residual variances and individual phenotypic variance, accuracy of progeny selection, restricted heritability, coefcient of individual additive genetic variation, coefcient of genotypic variation between progenies, coefcient of residual variation, coefcient of relative variation, variance of the prediction error of the genotypic values of the progeny), the prediction of genetic gains, and the selection of genotypes with fruits of better physical and chemical quality attributes. The carotenoid and β-carotene contents of the pulp were distinguished by high values of additive genetic variance, residual variance and individual variance. Additionally, genetic gains were achieved for the main carotenoid compounds, as xanthophyll (68%), lycopene (58%) and β-carotene (49%). In the second production cycle, using the technique of Mulamba and Mock, a new ranking method was used to select the best genotypes. These genetic parameters, especially restricted heritability, indicate the potential for advancing new selection stages to develop pumpkin cultivars with superior qualities, including fruit length (0.976), skin thickness (0.951) and orange pigments, particularly β-carotene (0.894). Additionally, frmer fruits with high ascorbic acid content and a pulp hue angle epresenting an orange color were ranked among the best genotypes.On-line

Aspectos agronomicos, silviculturais e economicos de sistemas agroflorestais implantados em areas de pastagens degradadas.

Desenvolvimentos de tecnologias para recuperacao de areas de pastagens abandonadas e degradadas por meio de sistemas agroflorestais, baseados em praticas de baixo insumos, que possam proporcionar desenvolvimento social, economico e ecologicamente sustentavel para o agricultor em Manaus-AM (Brasil)

Concentração séricas de selênio em cabras suplementadas com selênio orgânico durante a estação de monta.

Edición de las Memorias de la 25a. Reunión de la Asociación Latinoamericana de Producción Animal (ALPA), 2016, Recife, Brasil

Genomic characterization, high-density mapping and anchoring of DArT markers to the reference genome of Eucalyptus

Genetic linkage maps have been essential tools to examine the inheritance of qualitative and quantitative traits, to carry out comparative mapping and to provide markers for molecular breeding applications. Linkage maps for species of Eucalyptus have been reported for several pedigrees using different molecular marker technologies [1]. However improved marker density, throughput and transferability across species are necessary to increase resolution of current maps for a variety of genomic applications. We report the development of a high density linkage map for Eucalyptus based on microsatellites and DArT (Diversity Arrays Technology) markers generated by a standardized genotyping microarray [2]. DNA probes that constitute the DArT microarray were sequenced and positioned on the reference Eucalyptus genome providing information about their sequence content, their distribution relative to annotated genes as well as the relationship between physical and recombination distance in the Eucalyptus genome. [Poster Presentation

Propolis potential activity against Candida tropicalis adhered cells and its biofilms

Objectives: Invasive fungal infections, such as candidiasis, represent a public health problem of major importance, and Candida tropicalis has been highlighted among the main agents of candidiasis. One of the major contributions to C. tropicalis virulence is its versatility in adapting to a variety of different habitats and the formation of surface attached microbial communities known as biofilms. Moreover, from the clinical perspective, the most important feature of Candida biofilms is its role in increasing tolerance to conventional antifungal therapy. This scenario encourages the search for alternative therapies. Natural matrixes, such as propolis, compromise a multitude of bioactive properties, in particular phenolic extracts have evidenced significant antimicrobial properties against a multiple of opportunist invaders, including Candida species. Thus, the main objective of the present work was to evaluate the potential antifungal effect of propolis against Candida tropicalis biofilms. Methods: This study was conducted with four strains of C. tropicalis and one reference strain, from the American Type Culture Collection (ATCC 40042). Biofilm formation were carried out on 96-well microplates containing a cellular suspension of 1x105 cells/mL and incubated for 24 h at 37°C. Pre-formed C. tropicalis biofilms were treated with propolis (ranging from 0.47 to 1.42 mg/ml), during 24 h at 37°C and its effect assessed through quantification of the number of colony forming unit (CFU). Results: It was evident that all C. tropicalis strains tested were able to form biofilm and that propolis was able to reduce around 40% and 50% of the pre-formed biofilm. Moreover, in general the propolis effect was similar among all the C. tropicalis clinical isolates strains Conclusions: These data are promising, since they open important perspectives regarding new antifungal agents, much more effective and safer than the currently available to treat and to prevent C. tropicalis infections

Caatingueiro - Uma variedade de milho para o semi-árido nordestino.

bitstream/item/38505/1/cot29.pd

Heterologous mesenchymal stem cells successfully treat femoral pseudarthrosis in rats

<p>Abstract</p> <p>Background</p> <p>This study evaluated the effectiveness of treating pseudarthrosis in rats by using bone marrow cell suspensions or cultures of bone marrow mesenchymal stromal cells</p> <p>Methods</p> <p>Thirty-eight specific pathogen-free (SPF) animals were randomly assigned to four groups: Group 1, Control, without surgical intervention; Group 2 (Placebo), experimental model of femoral pseudarthrosis treated only with saline solution; Group 3, experimental model of femoral pseudarthrosis treated with heterologous bone marrow cells suspension; Group 4, experimental model of femoral pseudarthrosis treated with cultures of heterologous mesenchymal stromal cells from bone marrow. When pseudarthrosis was confirmed by simple radiological studies, digital radiography and histopathology after a 120-day postoperative period, Groups 2, 3 and 4 were treated as above. At 30, 60 and 90 days after the treatment, all animals were evaluated by simple radiological studies, and at the end of the experiment, the animals were assessed by computed axial tomography and anatomopathological and histomorphometric examinations.</p> <p>Results</p> <p>Injected cells were detected in the areas affected by pseudarthrosis using scintigraphy within the first 24 hours after their administration. After 60 days, the animals of Group 3 showed callus formation while the animals of Group 4 presented periosteal reaction and had some consolidated areas. In contrast, Group 2 showed a predominance of fibro-osteoid tissue. After 90 days, bone consolidation and remodeling was observed in all animals from Group 3 whereas animals from Group 4 exhibited partial consolidation and those ones from Group 2 persisted with pseudarthrosis.</p> <p>Conclusion</p> <p>The treatment with heterologous bone marrow cells suspension proved to be effective in the treatment of pseudarthrosis whereas cultures of heterologous bone marrow mesenchymal stromal cells did not show the same potential to aid bone healing.</p

Avaliação de serviços em saúde mental no Brasil: revisão sistemática da literatura

ResumoA avaliação em saúde mental é um mecanismo capaz de produzir informações que contribuam positivamente para a tomada de decisão na área. Logo, é preciso apropriar-se das discussões existentes, refletindo sobre desafios e possibilidades na produção de conhecimento neste campo. Realizou-se uma revisão sistemática da produção científica brasileira sobre avaliação de serviços em saúde mental, identificando e discutindo métodos, perspectivas avaliativas e resultados. A busca de artigos ocorreu nas bases de dados IBECS, Lilacs e Scielo, com recorte temporal da publicação da lei 10.216. Foram encontrados 35 artigos por meio dos descritores e critérios de inclusão e exclusão utilizados. A produção da área concentrou-se nas regiões Sul e Sudeste, com diversos âmbitos e participantes, visando contribuir para o aprimoramento de serviços e decisões na área. Destacam-se os avanços no cuidado, com tratamento humanizado, participativo e comunitário, mas carecendo de maiores investimentos, qualificação profissional e melhorias organizacionais. Postula-se maior integração entre pesquisas, com as avaliações ultrapassando aspectos estruturais e a comparação com modelos hospitalocêntricos