Arachidonic Acid as a Possible Negative Feedback Inhibitor of Nicotinic Acetylcholine Receptors on Neurons

Neuronal acetylcholine receptors, being highly permeable to calcium, are likely to regulate calcium-dependent events in neurons. Arachidonic acid is a membrane-permeant second messenger that can be released from membrane phospholipids by phospholipases in a calcium-dependent manner. We show here that activation of neuronal acetylcholine receptors triggers release of 3H-arachidonic acid in a calcium-dependent manner from neurons preloaded with the fatty acid. Moreover, low concentrations of arachidonic acid reversibly inhibit the receptors and act most efficiently on receptors likely to have the highest permeability to calcium, namely receptors containing α7 subunits. Low concentrations of arachidonic acid also reversibly inhibit α7- containing receptors expressed in Xenopus oocytes following injection of α7 cRNA. The oocyte results indicate following injection of α7 cRNA. The oocyte results indicate that the inhibition is a feature of the receptors rather than a consequence of neuron-specific machinery. The inhibition is not mediated by specific metabolites of arachidonic acid because the effects can be mimicked by other fatty acids; their effectiveness correlates with their content of double bonds. In contrast to arachidonic effects on calcium currents, inhibition of neuronal nicotinic receptors by the fatty acid cannot be prevented by blocking production of free radicals or by inhibiting protein kinase C. An alternative mechanism is that arachidonic acid binds directly to the receptors or perturbs the local environment in such a manner as to constrain receptor function

A fast numerical solution to the general mass-conservation equation for solutes and solids in aquatic sediments

Mathematical modeling of species transformations in aquatic sediments is usually based on numerical solutions to the same general one-dimensional mass-conservation equation and is likely to require substantial computation time. In this paper we present a fast numerical solution to this equation. The solution is suited for both single and multi-component models and it is based on an implicit control volume discretization of the general mass-conservation equation. The solution consists of two algorithms, one that decomposes the discretization matrix once and one that subsequently produces multiple solutions with minimal computational effort. A unique feature of these algorithms is that values of boundary conditions can vary as a simulation progresses without requiring new decompositions of the discretization matrix. This feature can reduce computation time significantly relative to commonly used procedures for modeling dynamic systems. Finally, we present four examples in which the numerical solution is applied to specific problems. From these examples guidelines are derived for the discretization in space and time required to obtain precise solutions of the general mass-conservation equation

Abū Qatāda al-Filasṭīnī versus IS: De ontwikkeling van een radicaal islamitisch geleerde

Door middel van een literatuurstudie is inzicht verkregen in de ideeën van Abū Qatāda al-Filasṭīnī en met name hoe deze ideeën zich verhouden tot zijn standpunt ten aanzien van IS. Dit is voornamelijk onderzocht met behulp van wetenschappelijke literatuur en Arabischtalige krantenartikelen. Het is gebleken dat hij de strijd van IS tegen de mujahidīn verwerpt omdat hiermee verdeeldheid onder moslims veroorzaakt wordt terwijl het juist eenheid zou moeten nastreven. Volgens Abū Qatāda is IS zijn naam, Islamitische Staat, niet waardig en kan dus gezien worden als één van de interne vijanden van de islam die bestreden dient te worden

Animal handling and stress-related behaviour at mobile slaughter of cattle

By avoiding animal transportation, mobile slaughter may have the potential to reduce animal stress. In a cross-sectional study with elements of cohort design, we investigated relationships between animal handling and stress-related animal behaviours in connection with slaughter at two Swedish slaughter plants: a newly started small-scale on-farm mobile abattoir and a relatively large-scale stationary slaughterhouse. To the stationary plant, the animals were transported on average 99 km from farms, and one third of these animals spent one night in lairage before slaughter. Data were collected during processing of 298 animals at both plants during one year. Stockperson actions and animal behaviours were observed in the driveways (2.4-7.3m long) to the stun box. Data on season, hour of day, air temperature, animal breed, animal category, animal age, carcass weight and stockperson category (plant or farm) were also collected. We used Spearman rank correlation, principal-component analysis and confirmatory factor analysis to develop a final generalised structural equation model, which contained six variables that represented observed animal backing; turning; slipping; eliminating; vocalising; and violent behaviour, regressed on a latent variable representing animal stress level. Stress level and animal behaviours were also regressed on five variables representing observed stockperson actions (touching, patting or hitting with hand; touching, patting or hitting with a tool; pushing; tail-twisting; and prodding with an electric goad) and on background variables including plant identity. The animal behaviours were modelled as counts per driveway length, clustered on farm identity. Marginal effects of stockperson actions and predictive margins of plants were calculated. The animals displayed backing and violent behaviour (kicking, goring or violent fighting) at significantly lower frequencies at the mobile plant than at the stationary one. In general, stockperson actions were significantly positively associated with animal behaviours, i.e. increased actions were associated with more frequent behaviours. Stockperson moving with tool, pushing and using electric goad were indirectly significantly associated with all animal behaviours via animal stress level. This study shows the importance of adequate cattle handling to limit pre-slaughter stress

Djurvälfärd vid mobil slakt av nötkreatur

Stark eller långvarig stress före slakt innebär sänkt djurvälfärd. En mobil slakterianläggning skulle kunna begränsa stressen bland annat genom att transporten till ett centralt beläget slakteri undviks. Under ett drygt år studerades djurvälfärden och köttkvaliteten vid slakt av nötkreatur i ett mobilt slakteri med slakt på ett storskaligt konventionellt slakteri som referens. De påvisade skillnaderna var huvudsakligen specifika för anläggningarna, snarare än generella för mobil och stationär slakt i allmänhet

Properties of Random Graphs with Hidden Color

We investigate in some detail a recently suggested general class of ensembles of sparse undirected random graphs based on a hidden stub-coloring, with or without the restriction to nondegenerate graphs. The calculability of local and global structural properties of graphs from the resulting ensembles is demonstrated. Cluster size statistics are derived with generating function techniques, yielding a well-defined percolation threshold. Explicit rules are derived for the enumeration of small subgraphs. Duality and redundancy is discussed, and subclasses corresponding to commonly studied models are identified.Comment: 14 pages, LaTeX, no figure

Scale-free networks without growth

In this letter, we proposed an ungrowing scale-free network model, wherein the total number of nodes is fixed and the evolution of network structure is driven by a rewiring process only. In spite of the idiographic form of $G$, by using a two-order master equation, we obtain the analytic solution of degree distribution in stable state of the network evolution under the condition that the selection probability $G$ in rewiring process only depends on nodes' degrees. A particular kind of the present networks with $G$ linearly correlated with degree is studied in detail. The analysis and simulations show that the degree distributions of these networks can varying from the Possion form to the power-law form with the decrease of a free parameter $\alpha$, indicating the growth may not be a necessary condition of the self-organizaton of a network in a scale-free structure.Comment: 4 pages and 3 figure

Long-term CO₂ production following permafrost thaw

Thawing permafrost represents a poorly understood feedback mechanism of climate change in the Arctic, but with a potential impact owing to stored carbon being mobilized1–5. We have quantified the long-term loss of carbon (C) from thawing permafrost in Northeast Greenland from 1996 to 2008 by combining repeated sediment sampling to assess changes in C stock and>12 years of CO2 production in incubated permafrost samples. Field observations show that the active-layer thickness has increased by>1 cm yr−1 but thawing has not resulted in a detectable decline in C stocks. Laboratory mineralization rates at 5 ◦C resulted in a C loss between 9 and 75%, depending on drainage, highlighting the potential of fast mobilization of permafrost C under aerobic conditions, but also that C at near-saturated conditions may remain largely immobilized over decades. This is confirmed by a three-pool

Formation of forest gaps accelerates C, N and P release from foliar litter during 4 years of decomposition in an alpine forest

Relative to areas under canopy, the soils in forest gaps receive more irradiance and rainfall (snowfall); this change in microclimate induced by forest gaps may influence the release of carbon (C) and nutrients during litter decomposition. However, great uncertainty remains about the effects of forest gaps on litter decomposition. In this study, we incubated foliar litters from six tree and shrub species in forest gaps and canopy plots and measured the release of C, nitrogen (N) and phosphorus (P) in different snow cover periods in an alpine forest from 2012 to 2016. We found that N was retained by 24-46% but that P was immediately released during an early stage of decomposition. However, forest gaps decreased litter N retention, resulting in more N and P being released from decomposing litters for certain species (i.e., larch, birch and willow litters). Moreover, the release of C and nutrients during litter decomposition stimulated by forest gaps was primarily driven by warmer soil temperature in this high-altitude forest. We conclude that gap formation during forest regeneration may accelerate C turnover and nutrient cycling and that this stimulation might be regulated by the litter species in this seasonally snow-covered forest.Peer reviewe