Sublethal effects induced by morphine to the freshwater biological model Dreissena polymorpha

Opioids are considered as emerging contaminants in aquatic ecosystems, mainly due to their large illicit consume worldwide. Morphine (MOR) is the main opiate and it was commonly found at measurable concentrations in freshwaters. Even though its occurrence is well documented, just limited information is available regarding its hazard to nontarget organisms. The aim of this study was of the evaluation of sublethal effects induced by MOR to the freshwater bivalve Dreissena polymorpha. We exposed mussels to two MOR concentrations (0.05 \ub5g/L and 0.5 \ub5g/L) for 14 days and we investigated the sublethal effects by a suite of biomarkers. The Neutral Red Retention Assay (NRRA) was used as a test of cytotoxicity, while the oxidative stress was evaluated by the activity of antioxidant and detoxifying enzymes, namely catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione-S-transferase (GST), and by measuring the levels of lipid peroxidation (LPO) and protein carbonylation (PCC). The genetic damage was assessed by the Single Cell Gel Electrophoresis (SCGE) assay, the DNA diffusion assay and the micronucleus test (MN test). Finally, the filtration rate of D. polymorpha was evaluated in order to investigate possible physiological effects. Both tested concentrations reduced the lysosome membrane stability of bivalves, but only the highest MOR concentration induced significant changes in the activity of antioxidant enzymes (SOD, CAT, and GPx) and increase in lipid peroxidation levels. Slight increase in primary DNA fragmentation was noticed, while no fixed genetic damage and alterations of the filtering rate were found

Manipulation of ovarian and uterine function to increase conception rates in cattle.

In cattle, embryonic and fetal losses are the major causes of reproductive failureo Losses associated with failure in the process of maternal recognition of pregnancy can amount to as much as 30%0 Successful establishment of pregnancy depends on a delicate balance between luteolytic mechanisms inherent to the endometrium at the end of diestrus and antiluteolytic mechanisms, orchestrated by the conceptus to change endometrial function and, ultimately, block luteolysis. Antiluteolytic strategies are pharmacological, mechanical, nutritional and management manipulations of the process of maternal recognition of pregnancy that aim to increase the probability of successful gestations. Objective of the present paper is to review the recent literature on antiluteolytic strategies in cattle. Specifically, we focused on strategies to stimulate production of progesterone, strategies to reduce production of estradiol and other sttategies. Future directions for research in this area are proposedo

Estratégias para reduzir a mortalidade embrionária em bovinos: I. Alternativas farmacológicas para otimizar a função luteínica de vacas de corte.

A mortalidade embrionária precoce é a maior causa de perdas reprodutivas em bovinos europeus criados no hemisfério norte. Uma vez que a eficiência reprodutiva do gado de corte nacional é baixa, conjetura-se que tais perdas também ocorram com bovinos de corte zebu criados no Brasil. Para aprofundar os conhecimentos sobre esse tema e propor possíveis estratégias que minimizem as perdas de prenhez, foi desenvolvido na Embrapa Pecuária Sudeste o projeto ?Mortalidade embrionária em bovinos e estratégias para a sua redução? que, além de ter como objetivo principal a determinação das taxas e da cronologia das perdas reprodutivas em vacas nelore inseminadas artificialmente, estabeleceu como meta o desenvolvimento de uma alternativa farmacológica de otimização da função luteínica de vacas de corte. Para tanto, avaliou-se o efeito de diferentes protocolos de suporte hormonal sobre as funções luteínicas e foliculares de vacas das raças Nelore e Red Angus. O alcance dessa meta, ou seja, o desenvolvimento de um protocolo de otimização da função luteínica de vacas de corte disponibiliza uma prática pecuária inovadora, a qual está descrita detalhadamente no presente Boletim de Pesquisa e Desenvolvimento.bitstream/item/43525/1/Boletim26.pd

Direction Specific Analysis of Psychoacoustics Parameters inside Car Cockpit: A Novel Tool for NVH and Sound Quality

Psychoacoustics parameters are widely employed in automotive field for objective evaluation of Sound Quality (SQ) of vehicle cabins and their components. The standard approach relies on binaural recordings from which numerical values and curves are calculated. In addition, head-locked binaural listening playback can be performed. The Virtual Reality (VR) technology recently started to diffuse also in automotive field, bringing new possibilities for enhanced and immersive listening sessions, thanks to the usage of massive microphone arrays instead of binaural microphones. In this paper, we combine both solutions: the principal SQ parameters are derived from multichannel recordings. This allows computing a map of direction-dependent values of SQ parameters. The acquisition system consists in a spherical microphone array with 32 capsules and a multiple-lens camera for capturing a panoramic equirectangular background image. The audio recording is encoded into High Order Ambisonics (HOA) format for being compared with a classic omnidirectional microphone and into Spatial PCM Sampling (SPS) format for producing 360° equirectangular color maps. The SPS encoding is used to plot over the background image the distribution of SPL values in dB (A) and of the SQ parameters: by adding to them the directional information, it results into a novel 360° diagnostic tool for localizing the most annoying sources. Furthermore, the playback of the HOA soundtrack can be performed both on a loudspeaker rig inside an Ambisonics listening room or on binaural headphones attached to a Head Mounted Display (HMD), benefiting from head-tracking and personalized Head Related Transfer Functions (HRTFs), allowing to make quick subjective evaluations with a degree of realism unattainable with the older static binaural approach

Removal of metallic elements from real wastewater using zebra mussel bio-filtration process

The metallic element pollution is a serious environmental problem but still unsolved since these contaminants are released mainly by human activity, reaching all the environmental compartments. Traditional wastewater treatment plants are very efficient in removing metallic elements only when their concentration is in the order of mg/L, but are not able to remove them until \u3bcg/L, as it would be needed to cope with the water quality standards in low flow receptors. Therefore, the aim of our study was to evaluate the potential removal of some recalcitrant metallic elements to the classical treatments, by the natural process of bio-filtration performed by the invasive zebra mussel (Dreissena polymorpha). For this purpose we built a pilot-plant at the Milano-Nosedo wastewater treatment plant, where we placed about 40,000 D. polymorpha specimens appointed to the wastewater bio-filtration. The metallic element removal due to zebra mussel activity was evaluated in the treated wastewater with a plasma optical emission spectrometry (ICP-OES). Data obtained in these experiments showed an encouraging metallic element removal due to D. polymorpha activity; in particular, the total abatement (100%) of Cr after one day of bio-filtration exposure is remarkable. Therefore, this study encourages further research related with the use of bivalves as a new tool for the wastewater depuration process; in this regard, the contaminated mollusks used in the bio-filtration could be incinerated or stored in special landfills, as is also the case of traditional sewage sludge