Chemical composition, in vitro dry matter digestibility and Gas production of four browse species and their combinations used as feed for small ruminants

Browse species as complete feed for ruminants is uncommon. This may be due to low dry matter (DM) and high condensed tannins (CT) contents limiting its potential to influence weight gain. Drying however improves DM content and reduces CT levels and its astringency. The objective of this study was to determine chemical composition, in vitro parameters and to evaluate the potential benefits of feeding small ruminants on dried browse leaves and their combinations. The browse species were Albizzia lebbek, Gliricidia sepium, Moringa oleifera and Millettia thoningii. Rumen fluid was obtained from two fistulated forest type wethers for the in vitro evaluation. The DM, crude protein (CP), ash, CT, neutral detergent fibre (NDF), acid detergent fibre (ADF), cellulose and lignin were 866-916 g/kg, 101-303 g/kg DM, 74.7-200 g/kg DM, 0.9-1.3 g/kg DM, 202-552 g/kg DM, 205-520 g/kg DM, 94-381 g/kg DM and 105-192 g/kg DM respectively. The organic matter digestibility (OMD), in vitro dry matter digestibility (IVDMD), in vitro gas production (IVGP), metabolisable energy (ME) and short chain fatty acids (SCFA) of the four browse species and their combinations recorded were 314.9-721.6 g/kg DM, 515.4-721 ml/g DM, 139-602 ml/g DM, 314.6-1406.9 ml/g DM and 3.1-14.4 ml/g DM respectively. There were positive associative effects shown by the combined browse leaves between IVDMD and IVGP. The regression analyses revealed that relationships between IVGP and CP and IVDMD and CT and all relationships between OMD, SCFA, ME and IVGP were significant (p<0.05). All the browse species and their combinations had IVDMD values of more than 500 g/kg DM and low gas production. The high CP and ash contents, low CTs, low to moderate fibre components, moderate to high IVDMD and low IVGP of the four browse species and their combinations make them potentially valuable as feed resources for small ruminant production

Assessment of the potential effect of some streams properties on the isolated Aeromonas hydrophila strains susceptibility against some β-Lactams and Sulfamids

The isolation and characterization of A. hydrophila strains were carried out a given stream located in the equatorial zone in Cameroon (Central Africa), during the mild rainy season and the mild dry season. The water physicochemical parameters measured were temperature, pH, electrical conductivity, color, turbidity and suspended solids. It has been noted that bacterial abundances as well as the values of physicochemical parameters undergoes temporal variations. Antimicrobial susceptibility tests showed that the means diameters of inhibition with Oxacillin, Ceftriazone, Penicellin, Sulfamethoxazol-trimethoprim, Chloramphenicol, Imipenem, Amoxicillin-Clavulanic acid and Cefazolin were 6.44, 21, 6.63, 9.58, 15.37, 22.18, 14.29 and 10.26 mm respectively. 100% of strains tested were resistant to Penicillin, 89.47 % resistant to Oxacillin, 68.42% resistant to Sulphamethoxazole-trimetroprim and 63.15% were resistant to Cefazolin. There were 54.60% cases of antibiotic resistance, 24.34 % of intermediate sensitivity and 21.05% of sensitive strains against the tested antibiotics. The encoded redundancy analysis indicates that the percentage of the variation explained on the canonical axes is of 47.8% for the F1 axis and 25.9% for the F2 axis, for an accumulated percentage of 73.7%. The suspended solids, the Ceftriazon and Chloramphenicol are correlated to the F1 axis in positive coordinates, and the Temperature and Amoxicillin-Clavulanic acid in negative coordinates. The correlation test between the water physicochemical characteristics and the inhibition diameters of antibiotics showed some significant correlations (P<0.05). The regulation of bacterial genes would be regulated by complex mechanisms. Although many factors implied are linked to the bacterial cell, others may belong to the closed environment

Team dynamics in emergency surgery teams: results from a first international survey

Background: Emergency surgery represents a unique context. Trauma teams are often multidisciplinary and need to operate under extreme stress and time constraints, sometimes with no awareness of the trauma\u2019s causes or the patient\u2019s personal and clinical information. In this perspective, the dynamics of how trauma teams function is fundamental to ensuring the best performance and outcomes. Methods: An online survey was conducted among the World Society of Emergency Surgery members in early 2021. 402 fully filled questionnaires on the topics of knowledge translation dynamics and tools, non-technical skills, and difficulties in teamwork were collected. Data were analyzed using the software R, and reported following the Checklist for Reporting Results of Internet E-Surveys (CHERRIES). Results: Findings highlight how several surgeons are still unsure about the meaning and potential of knowledge translation and its mechanisms. Tools like training, clinical guidelines, and non-technical skills are recognized and used in clinical practice. Others, like patients\u2019 and stakeholders\u2019 engagement, are hardly implemented, despite their increasing importance in the modern healthcare scenario. Several difficulties in working as a team are described, including the lack of time, communication, training, trust, and ego. Discussion: Scientific societies should take the lead in offering training and support about the abovementioned topics. Dedicated educational initiatives, practical cases and experiences, workshops and symposia may allow mitigating the difficulties highlighted by the survey\u2019s participants, boosting the performance of emergency teams. Additional investigation of the survey results and its characteristics may lead to more further specific suggestions and potential solutions

Effects of Ag presence on phases separation and order-disorder transitions in Cu-xAl-Mn alloys

The effects of Ag additions on phases separation and order-disorder transitions in the Cu-7Al-10Mn, Cu-9Al-10Mn and Cu-11Al-10Mn alloys have been studied using electrical resistivity measurements with temperature, differential scanning calorimetry, scanning electron and transmission electron microscopies, X-ray diffractometry and electron diffraction analysis. The results indicated that the decrease in Al content reduces the Cu 2 AlMn phase amount produced on slow cooling. It was also verified that the variation in Al fraction and Ag addition modify the order-disorder transitions temperatures. A schematic phase diagram showing the shift of phases field with addition of Ag has been proposed.Fil: Santos, C.M.A.. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Adorno, A.T.. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Stipcich, Marcelo Fernando. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Exactas. Instituto de Física de Materiales; Argentina. Universidade Federal de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; ArgentinaFil: Cuniberti, Adela Maria. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Exactas. Instituto de Física de Materiales; Argentina. Universidade Federal de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; ArgentinaFil: Souza, J.S.. Universidade Federal de Sao Paulo; BrasilFil: Bessa, C.V.X.. Universidade Federal de Sao Paulo; BrasilFil: Silva, R.A.G.. Universidade Federal de Sao Paulo; Brasi

Sensibilidade e resistência de amostras de Salmonella Typhimurium isoladas de suínos abatidos no Rio Grande do Sul/Brasil frente aos desinfetantes químicos quaternário de amônio e iodofor Sensitivity and resistance of samples of Salmonella Typhimurium isolated in slaughter swines in the state Rio Grande do Sul/Brazil, front to disinfectants quaternary ammonium and iodophor

Na prevenção da ocorrência ou na interrupção da evolução de enfermidades infecto-transmissíveis comuns aos animais e aos seres humanos, como é o caso da salmonelose, o uso de um desinfetante capaz de agir sobre o agente causal quando em vida livre, no ambiente, exerce grande importância. No entanto, a resistência microbiana, intrínseca ou adquirida, pode apresentar-se como um limitante no uso deste instrumento sanitário. Objetivando monitorar a sensibilidade da Salmonella Typhimurium, 96 amostras isoladas de suínos abatidos no Estado do Rio Grande do Sul,Brasil, foram confrontadas com dois compostos químicos desinfetantes (origem comercial) de uso freqüente em ambientes de produção animal e de transformação de seus subprodutos: um quaternário de amônio e o iodofor. Foram usadas as concentrações indicadas pelo fabricante e uma menor para simular possível situação de sub-concentração. O método de verificação foi o de diluição através do teste de suspensão, observando a inativação bacteriana nos tempos de contato 5, 15, 30 e 60 minutos. Como resultados obtidos, todas as amostras foram inativadas quando utilizado o composto quaternário de amônio, em ambas as concentrações. Frente ao iodofor, 4 (quatro) amostras mostraram-se resistentes a este composto na concentração indicada e 59 frente à sub-concentração. Conclui-se ser necessário, seja para a eleição ou para o monitoramento da eficácia, o confronto dos desinfetantes/anti-sépticos com bactérias presentes nos ambientes específicos de produção animal ou mesmo nos de transformação de seus subprodutos.<br>For prevention of infectious diseases common to man and animals such as salmonellosis, the successful use of disinfectants is of great importance. However, intrinsic or acquired resistance presented by microorganism against these compounds may constitute a limiting aspect in disinfections protocols. This study was aimed at monitoring the sensitivity of 96 Salmonella Typhimurium strains isolated from slaughter pigs in the state of Rio Grande do Sul, Brazil. The isolates were tested against quaternary ammonium and iodophor, which represent two commercial disinfectants commonly used in animal production. The tested disinfectants were used in the concentration recommended by the fabricant and in a sub-concentration in order to simulate a possible field situation. Dilution suspension tests were conducted, observing the inactivation of each S.Typhimurium isolate after 5, 15, 30 and 60 minutes of contact with each compound. All tested isolates were inactivated by the quaternary ammonium compound in both concentrations. Four isolates revealed resistant to iodophor in the recommended concentration and 59 isolates when a sub-concentration was tested. The testing of resistance against disinfectants in microorganisms present on farm and in food processing plants might be an important step on monitoring the effectiveness of adopted disinfections protocols