Clinical and magnetic resonance imaging characteristics of thoracolumbar intervertenral disk extrusions and protrusions in large breed dogs

It has recently been shown that the fat-derived hormone adiponectin has the ability to decrease hyperglycemia and to reverse insulin resistance. However, bacterially produced full-length adiponectin is functionally inactive. Here, we show that endogenous adiponectin secreted by adipocytes is post-translationally modified into eight different isoforms, as shown by two-dimensional gel electrophoresis. Carbohydrate detection revealed that six of the adiponectin isoforms are glycosylated. The glycosylation sites were mapped to several lysines (residues 68, 71, 80, and 104) located in the collagenous domain of adiponectin, each having the surrounding motif of GXKGE(D). These four lysines were found to be hydroxylated and subsequently glycosylated. The glycosides attached to each of these four hydroxylated lysines are possibly glucosylgalactosyl groups. Functional analysis revealed that full-length adiponectin produced by mammalian cells is much more potent than bacterially generated adiponectin in enhancing the ability of subphysiological concentrations of insulin to inhibit gluconeogenesis in primary rat hepatocytes, whereas this insulin-sensitizing ability was significantly attenuated when the four glycosylated lysines were substituted with arginines. These results indicate that full-length adiponectin produced by mammalian cells is functionally active as an insulin sensitizer and that hydroxylation and glycosylation of the four lysines in the collagenous domain might contribute to this activity.link_to_subscribed_fulltex

Candidate Rotating Toroids around High-Mass (Proto)Stars

Using the OVRO, Nobeyama, and IRAM mm-arrays, we searched for ``disk''-outflow systems in three high-mass (proto)star forming regions: G16.59-0.05, G23.01-0.41, and G28.87+0.07. These were selected from a sample of NH3 cores associated with OH and H2O maser emission and with no or very faint continuum emission. Our imaging of molecular line (including rotational transitions of CH3CN and 3mm dust continuum emission revealed that these are compact, massive, and hot molecular cores (HMCs), that is likely sites of high-mass star formation prior to the appearance of UCHII regions. All three sources turn out to be associated with molecular outflows from CO and/or HCO+ J=1--0 line imaging. In addition, velocity gradients of 10 -- 100 km/s per pc in the innermost densest regions of the G23.01 and G28.87 HMCs are identified along directions roughly perpendicular to the axes of the corresponding outflows. All the results suggest that these cores might be rotating about the outflow axis, although the contribution of rotation to gravitational equilibrium of the HMCs appears to be negligible. Our analysis indicates that the 3 HMCs are close to virial equilibrium due to turbulent pressure support. Comparison with other similar objects where rotating toroids have been identified so far shows that in our case rotation appears to be much less prominent; this can be explained by the combined effect of unfavorable projection, large distance, and limited angular resolution with the current interferometers.Comment: Accepted by ApJ main journal, the paper with the original quality figures are available from http://subarutelescope.org/staff/rsf/publication.htm

Gause's exclusion principle revisited: artificial modified species and competition

Gause's principle of competition between two species is studied when one of them is sterile. We study the condition for total extinction in the niche, namely, when the sterile population exterminates the native one by an optimal use of resources. A mathematical Lotka-Volterra non linear model of interaction between a native and sterile species is proposed. The condition for total extinction is related to the initial number $M_{o}$ of sterile individuals released in the niche. In fact, the existence of a critical sterile-population value $M_{c}$ is conjectured from numerical analysis and an analytical estimation is found. When spatial diffusion (migration) is considered a critical size territory is found and, for small territory, total extinction exist in any case. This work is motived by the extermination agriculture problem of fruit flies in our region.Comment: 11 pages. Published in Jour.Phys.A Math.Gen. 33, 4877 (2000

Strongly quadrature-dependent noise in superconducting micro-resonators measured at the vacuum-noise limit

We measure frequency- and dissipation-quadrature noise in superconducting lithographed microwave resonators with sensitivity near the vacuum noise level using a Josephson parametric amplifier. At an excitation power of 100~nW, these resonators show significant frequency noise caused by two-level systems. No excess dissipation-quadrature noise (above the vacuum noise) is observed to our measurement sensitivity. These measurements demonstrate that the excess dissipation-quadrature noise is negligible compared to vacuum fluctuations, at typical readout powers used in micro-resonator applications. Our results have important implications for resonant readout of various devices such as detectors, qubits and nano-mechanical oscillators.Comment: 13 pages, 4 figure

Phase preserving amplification near the quantum limit with a Josephson Ring Modulator

Recent progress in solid state quantum information processing has stimulated the search for ultra-low-noise amplifiers and frequency converters in the microwave frequency range, which could attain the ultimate limit imposed by quantum mechanics. In this article, we report the first realization of an intrinsically phase-preserving, non-degenerate superconducting parametric amplifier, a so far missing component. It is based on the Josephson ring modulator, which consists of four junctions in a Wheatstone bridge configuration. The device symmetry greatly enhances the purity of the amplification process and simplifies both its operation and analysis. The measured characteristics of the amplifier in terms of gain and bandwidth are in good agreement with analytical predictions. Using a newly developed noise source, we also show that our device operates within a factor of three of the quantum limit. This development opens new applications in the area of quantum analog signal processing

System-L amino acid transporters play a key role in pancreatic b-cell signalling and function

The branched-chain amino acids (BCAA) leucine, isoleucine and valine, are essential amino acids that play a critical role in cellular signalling and metabolism. They acutely stimulate insulin secretion and activate the regulatory serine/threonine kinase mammalian target of rapamycin complex 1 (mTORC1), a kinase that promotes increased β-cell mass and function. The effects of BCAA on cellular function are dependent on their active transport into mammalian cells via amino acid transporters and thus the expression and activity of these transporters likely influences β-cell signalling and function. In this report we show that the System-L transporters are required for BCAA uptake into clonal β-cell lines and pancreatic islets and that these are essential for signalling to mTORC1. Further investigation revealed that the System-L transporter LAT1 is abundantly expressed in islets and that knock-down of LAT1 using siRNA inhibits mTORC1 signalling, leucine-stimulated insulin secretion and islet cell proliferation. In summary, we show that the System-L transporter LAT1 is required for regulating β-cell signaling and function in islets and thus may be a novel pharmacological/nutritional target for the treatment and prevention of type-2 diabetes

evidence for polyclonal origin of multifocal clear cell renal cell carcinoma

Purpose: Renal cell carcinomas are often multifocal. We investigated the genomic signatures of multifocal clear cell renal cell carcinoma to determine whether multiple tumors in the same kidney bear a clonal relationship to one another. Experimental Design: A total of 62 tumors from 26 patients who underwent radical nephrectomy were examined. All patients had multiple separate clear cell renal carcinomas. Loss of heterozygosity analyses were done using five microsatellite polymorphic markers that represent putative tumor suppressor genes on chromosome 3p14 (D3S1300), 7q31 (D7S522), 8p22 (D8S261), 9p21 (D9S171), and 17p13 ( TP53 ). X chromosome inactivation analyses were also done on the renal tumors from the 10 female patients. Chromosome 3p deletion status was determined by dual color interphase fluorescence in situ hybridization analysis in all tumors. Results: Nineteen of the 26 (73%) patients with multifocal clear cell renal cell carcinoma showed allelic loss in at least 1 of 5 microsatellite loci in separate tumors analyzed. A disconcordant pattern of allelic loss between coexisting kidney tumors was observed in 7 cases. Six cases showed discordant 3p deletion patterns by dual color interphase fluorescence in situ hybridization analysis. Of the eight informative female cases studied by X chromosome inactivation, one showed a discordant nonrandom pattern of X chromosome inactivation. Overall, evidence of independent origin of the multifocal renal tumors was observed in 12 of 26 cases (46%). Conclusions: Our data suggest that in a significant number of cases of multifocal clear cell renal cell carcinoma, the spatially separate tumors are of different clonal origin and arise independently

Evaluation of the fineness of degummed bast fibers

Fiber fineness characteristics are important for yarn production and quality. In this paper, degummed bast fibers such as hemp, flax and ramie have been examined with the Optical Fiber Diameter Analyzer (OFDA100 and OFDA2000) systems for fiber fineness, in comparison with the conventional image analysis and the Wira airflow tester. The correlation between the results from these measurements was analysed. The results indicate that there is a significant linear co-relation between the fiber fineness measurement results obtained from those different systems. In addition, the mean fiber width and its coefficient of variation obtained from the OFDA100 system are smaller than those obtained from the OFDA2000 system, due to the difference in sample preparation methods. The OFDA2000 system can also measure the fiber fineness profile along the bast fiber plants, which can be useful for plant breeding. <br /

A Monte Carlo simulation of the Sudbury Neutrino Observatory proportional counters

The third phase of the Sudbury Neutrino Observatory (SNO) experiment added an array of 3He proportional counters to the detector. The purpose of this Neutral Current Detection (NCD) array was to observe neutrons resulting from neutral-current solar neutrino-deuteron interactions. We have developed a detailed simulation of the current pulses from the NCD array proportional counters, from the primary neutron capture on 3He through the NCD array signal-processing electronics. This NCD array Monte Carlo simulation was used to model the alpha-decay background in SNO's third-phase 8B solar-neutrino measurement.Comment: 38 pages; submitted to the New Journal of Physic