Heterochronic faecal transplantation boosts gut germinal centres in aged mice

Ageing is a complex multifactorial process associated with a plethora of disorders, which contribute significantly to morbidity worldwide. One of the organs significantly affected by age is the gut. Age-dependent changes of the gut-associated microbiome have been linked to increased frailty and systemic inflammation. This change in microbial composition with age occurs in parallel with a decline in function of the gut immune system, however it is not clear if there is a causal link between the two. Here we report that the defective germinal centre reaction in Peyer’s patches of aged mice can be rescued by faecal transfers from younger adults into aged mice and by immunisations with cholera toxin, without affecting germinal centre reactions in peripheral lymph nodes. This demonstrates that the poor germinal centre reaction in aged animals is not irreversible, and that it is possible to improve this response in older individuals by providing appropriate stimuli

Suppressing bladder artifacts in bone SPECT of the pelvis.

OBJECTIVE: Bladder-filling reconstruction artifacts have a detrimental effect on the image quality of pelvic bone single photon emission computed tomography (SPECT). Using a simple protocol consisting of forced diuresis coupled with intravenous (IV) hydration, this study was undertaken to obtain an artifact-free pelvic SPECT after discarding the residual urinary activity. METHODS: Thirty patients were enrolled. In group I, pelvic SPECT was performed directly after normal void, whereas in group II, SPECT was preceded by IV injection of 0.5 mg/kg furosemide (maximum 40 mg) coupled with IV infusion of 500 cc of physiologic saline. Bladder-filling reconstruction artifacts were analyzed in group I patients, who had their images reconstructed using both filtered backprojection and iterative algorithms, both qualitatively and quantitatively by means of regions of interest (ROIs) drawn around the artifact-bearing bone areas as well as the corresponding contralateral sites. For group II patients, besides visual analysis, ROIs were placed over the sites corresponding to those of the group I patients. In every patient, total counts of each ROI were normalized to a reference ROI placed over the sacrum, and a ratio was created. RESULTS: Using filtered backprojection, two forms of artifacts were identified in group I patients: first, a streak pattern that extended to the sacro-iliac joint in nine (60%) patients, the hip joint in five (33%), the superior pubic rami in four (27%), the sacrum in three (20%), and the ischium in one (6%); second, a count loss subtype which extended to the hip joints in nine (60%) patients. Corresponding values after iterative reconstruction were two (13%) for the sacro-iliac joint, three (20%) for the hip joint, one (6%) for the superior pubic ramus, and one (6%) for the sacrum. In five (33%) patients, residual count loss artifacts were still identifiable after iterative reconstruction. However in group II, no such effects were observed because the bladder activity reached near background level in 14 (93%) of 15 patients after three successive voids with a 3.5-fold decrease in the mean value of total bladder count in comparison with group I patients. A statistically significant difference was found between artifact- and non-artifact-harboring ROIs in group I whichever the method used for reconstruction, whereas the values of right and left hemi-pelvis ROIs/sacrum in group II were almost identical. CONCLUSIONS: Forced diuresis coupled with parenteral hydration facilitates the acquisition of an artifact-free pelvic SPECT. Especially for clinical questions that focus on femoral heads and pubic bones, applying the aforementioned protocol may improve the diagnostic accuracy of pelvic bone SPECT

The ionizing photon production efficiency at z ∼6 for Lyman-Alpha emitters using JEMS and MUSE

We study the ionizing photon production efficiency at the end of the Epoch of Reionization (z ∼5.4-6.6) for a sample of 30 Ly α emitters. This is a crucial quantity to infer the ionizing photon budget of the universe. These objects were selected to have reliable spectroscopic redshifts, assigned based on the profile of their Ly α emission line, detected in the MUSE deep fields. We exploit medium-band observations from the JWST Extragalactic Medium-band Survey (JEMS) to find the flux excess corresponding to the redshifted Hα emission line. We estimate the ultraviolet (UV) luminosity by fitting the full JEMS photometry, along with several HST photometric points, with Prospector. We find a median UV continuum slope of, indicating young stellar populations with little-To-no dust attenuation. Supported by this, we derive ζion,0 with no dust attenuation and find a median value of log. If we perform dust attenuation corrections and assume a Calzetti attenuation law, our values are lowered by ∼0.1 dex. Our results suggest Ly α emitters at the Epoch of Reionization have slightly enhanced ζion,0 compared to previous estimations from literature, in particular, when compared to the non-Ly α emitting population. This initial study provides a promising outlook on the characterization of ionizing photon production in the early universe. In the future, a more extensive study will be performed on the entire data set provided by the JWST Advanced Deep Extragalactic Survey (JADES). Thus, for the first time, allowing us to place constraints on the wider galaxy populations driving reionization

At the coalface and the cutting edge: general practitioners’ accounts of the rewards of engaging with HIV medicine

The interviews we conducted with GPs suggest that an engagement with HIV medicine enables clinicians to develop strong and long-term relationships with and expertise about the care needs of people living with HIV ‘at the coalface’, while also feeling connected with a broader network of medical practitioners and other professionals concerned with and contributing to the ever-changing world of science: ‘the cutting edge’. The general practice HIV prescriber is being modelled here as the interface between these two worlds, offering a rewarding opportunity for general practitioners to feel intimately connected to both community needs and scientific change

JEMS: A Deep Medium-band Imaging Survey in the Hubble Ultra Deep Field with JWST NIRCam and NIRISS

We present JWST Extragalactic Medium-band Survey, the first public medium-band imaging survey carried out using JWST/NIRCam and NIRISS. These observations use ∼2 and ∼4 μm medium-band filters (NIRCam F182M, F210M, F430M, F460M, F480M; and NIRISS F430M and F480M in parallel) over 15.6 arcmin2 in the Hubble Ultra Deep Field (UDF), thereby building on the deepest multiwavelength public data sets available anywhere on the sky. We describe our science goals, survey design, NIRCam and NIRISS image reduction methods, and describe our first data release of the science-ready mosaics, which reach 5σ point-source limits (AB mag) of ∼29.3-29.4 in 2 μm filters and ∼28.2-28.7 at 4 μm. Our chosen filters create a JWST imaging survey in the UDF that enables novel analysis of a range of spectral features potentially across the redshift range of 0.3 1 mag) across redshifts 1.5 < z < 9.3, most prominently Hα+[N ii] and [O iii]+Hβ. We present our first data release including science-ready mosaics of each medium-band image available to the community, adding to the legacy value of past and future surveys in the UDF. This survey demonstrates the power of medium-band imaging with JWST, informing future extragalactic survey strategies using JWST observations

Inside the bubble: exploring the environments of reionisation-era Lyman- α emitting galaxies with JADES and FRESCO*

We present a study of the environments of 17 Lyman-α emitting galaxies (LAEs) in the reionisation-era (5.8 5%) observed in our sample of LAEs, suggesting the presence of ionised hydrogen along the line of sight towards at least eight out of 17 LAEs. We find minimum physical 'bubble'sizes of the order of Rion ∼ 0.1- 1pMpc are required in a patchy reionisation scenario where ionised bubbles containing the LAEs are embedded in a fully neutral IGM. Around half of the LAEs in our sample are found to coincide with large-scale galaxy overdensities seen in FRESCO at z ∼ 5.8- 5.9 and z ∼ 7.3, suggesting Lyman-α transmission is strongly enhanced in such overdense regions, and underlining the importance of LAEs as tracers of the first large-scale ionised bubbles. Considering only spectroscopically confirmed galaxies, we find our sample of UV-faint LAEs (MUV ≳ -20mag) and their direct neighbours are generally not able to produce the required ionised regions based on the Lyman-α transmission properties, suggesting lower-luminosity sources likely play an important role in carving out these bubbles. These observations demonstrate the combined power of JWST multi-object and slitless spectroscopy in acquiring a unique view of the early Universe during cosmic reionisation via the most distant LAEs

A recently quenched galaxy 700 million years after the Big Bang

Local and low-redshift (z  1010 M⊙) and relatively old. Here we report a (mini-)quenched galaxy at z = 7.3, when the Universe was only 700 Myr old. The JWST/NIRSpec spectrum is very blue (U–V = 0.16 ± 0.03 mag) but exhibits a Balmer break and no nebular emission lines. The galaxy experienced a short starburst followed by rapid quenching; its stellar mass (4–6 × 108 M⊙) falls in a range that is sensitive to various feedback mechanisms, which can result in perhaps only temporary quenching

JADES: Probing interstellar medium conditions at z ∼ 5.5-9.5 with ultra-deep JWST/NIRSpec spectroscopy

We present emission-line ratios from a sample of 27 Lyman-break galaxies from z∼ 5.5-9.5 with-17.0 5.5 spectra. We find that the emission-line ratios exhibited by these z∼ 5.5-9.5 galaxies occupy clearly distinct regions of line-ratio space compared to typical z∼ 0-3 galaxies, instead being more consistent with extreme populations of lower-redshift galaxies. This is best illustrated by the [O III]/[O II] ratio, tracing interstellar medium (ISM) ionisation, in which we observe more than half of our sample to have [O III]/[O II] > 10. Our high signal-to-noise spectra reveal more than an order of magnitude of scatter in line ratios such as [O II]/Hβ and [O III]/[O II], indicating significant diversity in the ISM conditions within the sample. We find no convincing detections of [N II] λ6583 in our sample, either in individual galaxies, or a stack of all G395M/F290LP spectra. The emission-line ratios observed in our sample are generally consistent with galaxies with extremely high ionisation parameters (log U∼-1.5), and a range of metallicities spanning from ∼0.1 × Z⊙ to higher than ∼0.3 × Z⊙, suggesting we are probing low-metallicity systems undergoing periods of rapid star formation, driving strong radiation fields. These results highlight the value of deep observations in constraining the properties of individual galaxies, and hence probing diversity within galaxy population

Carbonaceous dust grains seen in the first billion years of cosmic time

Large dust reservoirs (up to approximately 108 M ⊙) have been detected1–3 in galaxies out to redshift z ≃ 8, when the age of the Universe was only about 600 Myr. Generating substantial amounts of dust within such a short timescale has proven challenging for theories of dust formation4,5 and has prompted the revision of the modelling of potential sites of dust production6–8, such as the atmospheres of asymptotic giant branch stars in low-metallicity environments, supernova ejecta and the accelerated growth of grains in the interstellar medium. However, degeneracies between different evolutionary pathways remain when the total dust mass of galaxies is the only available observable. Here we report observations of the 2,175 Å dust attenuation feature, which is well known in the Milky Way and galaxies at z ≲ 3 (refs. 9–11), in the near-infrared spectra of galaxies up to z ≃ 7, corresponding to the first billion years of cosmic time. The relatively short timescale implied for the formation of carbonaceous grains giving rise to this feature12 suggests a rapid production process, possibly in Wolf–Rayet stars or supernova ejecta