Comunicación corta. Aflatoxina y ochratoxina A totales en hígado, riñones y plasma de pollos contaminados experimentalmente

Mycotoxicosis, particularly that caused by aflatoxins and ochratoxins, is a serious problem for the poultry production industry. The aim of this study was to determine the total aflatoxin and ochratoxin A levels in liver and plasma, and kidneys and plasma, respectively, of chickens for fattening fed experimentally contaminated diets, and to assess the impact of these toxins on body weight increase and the feed conversion ratio (FCR). Forty eight 21 day-old Ross breeder chickens (n = 12 x four treatments) were fed diets containing different levels of mycotoxins: grower diet + ochratoxin A (200 mg/kg of feed) + total aflatoxin in different concentrations (T1 = 60 microg/kg of feed; T = 50 microg/kg; T3 = 30 microg/kg). Control chickens (T4) were fed only the grower diet. No significant differences were seen in the weight increase of chickens subjected to the different treatments. However, lower FCRs were seen in those exposed to T1 and T2. In general, the amount of total aflatoxin and ochratoxin A found in the liver, kidneys and plasma (determined by ELISA) were directly related to the amounts added to the experimental diets and feed consumption. Plasma ochratoxin A was always higher than kidney levels; this relationship was not seen for total aflatoxin.Las micotoxicosis, en particular las aflatoxicosis y ocratoxicosis, son un serio problema sanitario en la producción avícola. El objetivo de este trabajo fue determinar el nivel de aflatoxinas y ochratoxinas A en hígado y plasma, y riñones y plasma, respectivamente, de pollos alimentados con dietas experimentalmente contaminadas e investigar el impacto de estas toxinas en el peso corporal y el cociente de conversión del alimento (FCR). Se alimentaron 48 pollos Ross de 21 días de edad (n = 12 x cuatro tratamientos) con dietas conteniendo diferentes niveles de micotoxinas: una dieta de crecimiento + 200 mg/kg de ochratoxina A + aflatoxina total en diferentes concentraciones (T1 = 60 microg/kg; T2 = 50 microg/kg; T3 = 30 microg/kg). Lo pollos control (T4) fueron solamente alimentados con la dieta de engorde. No hubo diferencias significativas en el aumento de peso de los pollos sometidos a los diferentes tratamientos; sin embargo, se detectaron FCRs menores para los expuestos a T1 y T2. En general, la cantidad de aflatoxina total y ochratoxina A detectadas por ELISA en hígado, riñones y plasma fueron directamente proporcionales a los niveles de las micotoxinas añadidas a las dietas experimentales. La ochratoxina A en plasma fue siempre superior a la encontrada en riñón; esta relación no se detectó para la aflatoxina total

Common host-derived chemicals increase catches of disease-transmitting mosquitoes and can improve early warning systems for rift valley fever virus

Rift Valley fever (RVF), a mosquito-borne zoonosis, is a major public health and veterinary problem in sub-Saharan Africa. Surveillance to monitor mosquito populations during the inter-epidemic period (IEP) and viral activity in these vectors is critical to informing public health decisions for early warning and control of the disease. Using a combination of field bioassays, electrophysiological and chemical analyses we demonstrated that skin-derived aldehydes (heptanal, octanal, nonanal, decanal) common to RVF virus (RVFV) hosts including sheep, cow, donkey, goat and human serve as potent attractants for RVFV mosquito vectors. Furthermore, a blend formulated from the four aldehydes and combined with CO2- baited CDC trap without a light bulb doubled to tripled trap captures compared to control traps baited with CO2 alone. Our results reveal that (a) because of the commonality of the host chemical signature required for attraction, the host-vector interaction appears to favor the mosquito vector allowing it to find and opportunistically feed on a wide range of mammalian hosts of the disease, and (b) the sensitivity, specificity and superiority of this trapping system offers the potential for its wider use in surveillance programs for RVFV mosquito vectors especially during the IEP.The support through a scholarship to DPT by the German Academic Exchange Service (DAAD) is greatly acknowledged. This research was funded by Google.org, the philanthropic arm of Google. We are grateful to Francis Mulwa and James Wauna for assistance in field experiments and other individuals at the communities who helped in the same capacity. We acknowledge the technical assistance of Dunston Betti, John Gachoya and Joel Lutomiah, Kenya Medical Research Institute (KEMRI) and James Mutysia of Walter Reed Project (WRP), Nairobi. Our sincere gratitude to Dr. Salifu Daisy, Mrs Caroline Tigoi, and Mr. Benedict Orindi, International Centre of Insect Physiology and Ecology (icipe), Nairobi. We appreciate the services of the chiefs at the trapping communities both at Marigat and Ijara districts. The Arbovirus Incidence and Diversity Project consortium-icipe, International Livestock Research Institute (ILRI), Kenya Agricultural Research Institute (KARI), Kenya Ministry of Livestock-Department of Veterinary Services (DVS), KEMRI, Kenya Ministry of Public Health and Sanitation and Kenya Wildlife Service (KWS).www.plosntds.orgam2013ab201

Multi-locus sequence typing of African swine fever viruses from endemic regions of Kenya and Eastern Uganda (2011–2013) reveals rapid B602L central variable region evolution

The central variable region (CVR) within the B602L gene of the African swine fever virus (ASFV) is highly polymorphic within the 23 ASFV genotypes defined by sequencing of the C-terminal end of the p72 locus. Sequencing the p54 gene further discriminates ASFV genotypes that are conserved at the p72 locus. Variation in the thymidine kinase locus is a novel additional tool for ASFV genotyping whose application for this purpose is described for the first time herein. We evaluated genetic variation at these four polymorphic loci in 39 ASFV isolates obtained from outbreaks in Kenya and a region of Eastern Uganda between 2011 and 2013. Analysis of the p72 and p54 loci revealed high genetic conservation among these isolates; all clustered within p72 genotype IX and were similar to isolates associated with earlier outbreaks in East Africa. The thymidine kinase gene of the Kenyan isolates in this study were distinct relative to Southern African isolates and synonymous substitutions were observed among viruses from central Kenya. Analysis of the CVR within the B602L gene revealed two previously unknown polymorphisms that were restricted to Western Kenya and Eastern Uganda. A novel variant was revealed within CVR subgroup XXIV and a novel CVR subgroup XXIVa that contains tetrameric repeat F which has previously only been associated with p72 genotype I, was also identified for the first time in East Africa. Phylogeographic analysis of isolates based on CVR polymorphisms revealed rapid evolution and dissemination of variants present within ASFV genotype IX in East Africa.Supplementary Fig. 1 Phylogenetic tree based on the C-terminal end of the p72 protein comparing the Kenyan and Eastern Uganda ASFV isolates collected in this study (●) between 2011 and 2013 with other African swine fever virus isolates belonging to ASFV genotypes IX and X. A total of 91 distinct taxa were used to infer a Minimum Evolution tree and the percentage of replicate trees in which the associated taxa clustered together in a bootstrap analysis (1000 replicates) are shown adjacent to the branches. The tree is drawn to scale; with branch lengths represented using the same units as the evolutionary distances used to infer the phylogenetic tree.Supplementary Fig. 2 Phylogenetic tree highlighting genetic conservation within the E183L gene within the Kenyan and Eastern Uganda ASFV isolates in comparison to reference nucleotide sequences obtained from GenBank.Supplementary Fig. 3 Amino acid sequences translated using SeqPublish highlighting synonymous substitutions within the thymidine kinase gene in the ASFV isolates obtained from Central Kenya.Supplementary Table 1 Summary of the data obtained from ASFV isolates selected for genotyping in this study and the respective GenBank accession numbers.The Australian aid (AusAID) and the Commonwealth Scientific and Industrial Research Organization (CSIRO) under the Special Africa Program.http://link.springer.com/journal/11262hj2018Mammal Research InstituteZoology and Entomolog

Detection of Inconspicuous Power Quality Disturbances through Step Changes in rms Voltage Profile

Power quality disturbances commonly observed in power systems have been studied for decades, resulting in numerous algorithms for detecting the events that affect the voltage and/or current waveforms. However, a considerable amount of disturbances is not visually observable in the raw waveforms, especially switching operations. These events must be detected through an alternative feature, such as abrupt variations in the root-mean-square (rms) voltage profile. This study examines the methods commonly used for detecting power quality disturbances in the waveform or rms voltage profile domains and identifies their limitations. Afterwards, a novel step change detector is proposed based on a modified median filter and rms voltage gradient values to overcome the deficiencies of the existing methods. The effectiveness of the proposed method is assessed by applying it to both simulated and field data. This assessment shows that the method detects all switching events with no false positives for the datasets under analysis

Manejo e viabilidade econômica da irrigação por gotejamento na cultura do cafeeiro acaiá considerando seis safras

Este trabalho teve como objetivo estudar técnica e economicamente o efeito da irrigação por gotejamento na produção do cafeeiro (Coffea arabica L.) Acaiá MG-1474. O delineamento experimental utilizado foi o de blocos casualizados, com cinco tratamentos e quatro repetições. Os tratamentos foram: L0 = sem irrigação; L40 = 40%; L60 = 60%; L80 = 80% e L100 = 100% da Evaporação do Tanque Classe A (ECA). Para realizar a análise econômica, utilizou-se dos dados de produção acumulada das seis primeiras safras: 1999, 2000, 2001, 2002, 2003 e 2004. A análise dos custos da lavoura irrigada foi baseada na teoria dos custos de produção. A produtividade média dos tratamentos foi de 52,88; 66,99; 70,00; 71,93 e 79,50 sacas por hectare, para L0, L40, L60, L80 e L100, respectivamente. Considerando o preço da saca de café a R$212,00, a situação econômica analisada mostrou que, neste nível de preço, é economicamente viável a produção de café, quer seja irrigado, quer não, sendo que, para os tratamentos irrigados, o manejo da irrigação, com a lâmina de reposição de 100$ 212.00 per bag, the coffee production is practicable, for irrigated and non irrigated crops. A water replacement of 100% of the evaporation provided the best profit

Sample size for full-sib family evaluation in sugarcane

The objective of this study was to determine the minimum number of plants per plot that must be sampled in experiments with sugarcane (Saccharum officinarum) full-sib families in order to provide an effective estimation of genetic and phenotypic parameters of yield-related traits. The data were collected in a randomized complete block design with 18 sugarcane full-sib families and 6 replicates, with 20 plants per plot. The sample size was determined using resampling techniques with replacement, followed by an estimation of genetic and phenotypic parameters. Sample-size estimates varied according to the evaluated parameter and trait. The resampling method permits an efficient comparison of the sample-size effects on the estimation of genetic and phenotypic parameters. A sample of 16 plants per plot, or 96 individuals per family, was sufficient to obtain good estimates for all traits considered of all the characters evaluated. However, for Brix, if sample separation by trait were possible, ten plants per plot would give an efficient estimate for most of the characters evaluated.O objetivo deste estudo foi determinar o número mínimo de plantas por parcela a ser amostrado em experimentos de famílias de irmãos completos, em cana-de-açúcar (Saccharum officinarum), para possibilitar a estimação eficiente de parâmetros genéticos e fenotípicos para características de produção. Os dados foram coletados em delineamento de blocos ao acaso, composto por 18 famílias de irmãos completos, com 6 repetições e 20 plantas por parcela. O tamanho da amostra foi determinado com o uso de técnicas de reamostragem com reposição, com posterior estimação dos parâmetros genéticos e fenotípicos. As estimativas do tamanho da amostra variaram de acordo com a variável e o parâmetro avaliados. O método da reamostragem permite uma comparação eficiente dos efeitos do tamanho da amostra na estimação de parâmetros genéticos e fenotípicos. Uma amostra de 16 plantas por parcela, ou seja, 96 indivíduos, por família, foi suficiente para obter estimativas fidedignas de todos os parâmetros avaliados em todas as variáveis consideradas. Porém, para a variável Brix, se fosse possível desmembrar a amostragem por característica, uma amostra de dez plantas por parcela já possibilitaria a estimação precisa da maioria dos parâmetros genéticos e fenotípicos avaliados