Generalized model of blockage in particulate flow limited by channel carrying capacity

We investigate stochastic models of particles entering a channel with a random time distribution. When the number of particles present in the channel exceeds a critical value $N$, a blockage occurs and the particle flux is definitively interrupted. By introducing an integral representation of the $n$ particle survival probabilities, we obtain exact expressions for the survival probability, the distribution of the number of particles that pass before failure, the instantaneous flux of exiting particle and their time correlation. We generalize previous results for $N=2$ to an arbitrary distribution of entry times and obtain new, exact solutions for $N=3$ for a Poisson distribution and partial results for $N\ge 4$.Comment: 13 pages, 9 figure

Genetic Changes Over Breeding Generations of \u3cem\u3eFestulolium\u3c/em\u3e

Festulolium hybrids are a valuable breeding source for tolerance to abiotic stress and to make grass more persistent under drought and in cold environments. In 2004, the EU Commission enlarged the definition of Festulolium which may now include all hybrids between Lolium sp. and Festuca sp. and not only those between L. multiflorum and F. pratensis. We here report allele frequencies at two unlinked PCR-based marker loci in populations derived from tetraploid (2n=4x=28) L. multiflorum x F. glaucescens hybrids where breeding history enables us to test the effects of selection vs that of genetic drift

The Effect of Blue Light on Leaf Growth and Plant Development in Two Morphologically Contrasted Perennial Ryegrass Genotypes: Cellular Basis and Ecological Implications

Several major plant responses to competition for light are determined by responses to light signals, in particular to red/far-red ratio (R/FR) and blue light, besides responses mediated through photosynthesis and carbon assimilation (Gautier et al., 1999). These responses to light signals allow plants to react to the presence of neighbours and to anticipate the impact of light competition on photosynthesis. The objective of the study was to evaluate the impact of blue light on leaf growth and its cellular basis, on two short and long leaved populations (FC and FL respectively), which were shown to have different competitive ability (Hazard et al., 1996)

Experimental study of granular surface flows via a fast camera: a continuous description

Depth averaged conservation equations are written for granular surface flows. Their application to the study of steady surface flows in a rotating drum allows to find experimentally the constitutive relations needed to close these equations from measurements of the velocity profile in the flowing layer at the center of the drum and from the flowing layer thickness and the static/flowing boundary profiles. The velocity varies linearly with depth, with a gradient independent of both the flowing layer thickness and the static/flowing boundary local slope. The first two closure relations relating the flow rate and the momentum flux to the flowing layer thickness and the slope are then deduced. Measurements of the profile of the flowing layer thickness and the static/flowing boundary in the whole drum explicitly give the last relation concerning the force acting on the flowing layer. Finally, these closure relations are compared to existing continuous models of surface flows.Comment: 20 pages, 11 figures, submitted to Phys. FLuid

Estimation of the Coefficient of Double-Reduction in Autotetraploid Lucerne

Polyploidy plays an important role in the evolution of species and many cultivated species, particularly in angiosperms, are polyploids (Bever and Felber, 1992; Gallais, 2003). Autopolyploid species that show a tetrasomic inheritance have complex genetics. However, some theoretical models were built for: (1) genetic mapping (Hackett et al., 1998), (2) quantitative genetics (Gallais, 2003) and (3) population genetics (Ronfort et al., 1998). But in practice, most data analyses ignore one essential feature of tetrasomic inheritance that is double-reduction. Indeed, in an autotetraploid species, homologous chromosomes can form tetravalents at meiosis. In this case, a double-reduction is observed if crossing-over occurs between a locus and its centromere, and if the sister chromatids migrate to the same pole at anaphase I. The gametes may, thus, carry a pair of sister alleles. Double-reduction frequency is represented by the index of separation (α) (Demarly, 1963; Mather, 1935; Mather, 1936). The parameter α is considered as a product of four probabilities: α = q e a s (Gallais, 2003) in which “ q ” is the probability of multivalent formation, “ e ” the probability of first equational division, related to the frequency of crossing-over, “ a ” is the probability of non-disjunction at first anaphase and “s” is the probability of having two sister chromatids in the same gamete. If separation during anaphase II is random, s = ½. Consequently, α will be low for a gene located in the vicinity of the centromere and will increase with distance between the gene and the centromere. It was demonstrated that double-reduction events alter the rate of progression towards equilibrium under inbreeding or under random mating, modify the recombination rate between loci and also alter the rate of decay of linkage disequilibrium under random mating (Bever and Felber, 1992). Current theoretical models allow drawing genetic maps taking into account double-reduction (Luo et al., 2004). Thus, it is possible to estimate α for codominant loci in tetraploid species. To date, we have few estimates of the double-reduction frequency. Haynes and Douches (1993) on potato and Julier et al. (2003) on lucerne found that double-reduction occurs sporadically. In both studies, the low number of progenies hampered a precise estimation of α. The aim of our study was to estimate the frequency of double-reduction in a mapping population of lucerne that includes a large number of individuals

Mannose-Specific Lectins from Marine Algae: Diverse Structural Scaffolds Associated to Common Virucidal and Anti-Cancer Properties.

To date, a number of mannose-specific lectins have been isolated and characterized from seaweeds, especially from red algae. In fact, man-specific seaweed lectins consist of different structural scaffolds harboring a single or a few carbohydrate-binding sites which specifically recognize mannose-containing glycans. Depending on the structural scaffold, man-specific seaweed lectins belong to five distinct structurally-related lectin families, namely (1) the griffithsin lectin family (beta-prism I scaffold); (2) the Oscillatoria agardhii agglutinin homolog (OAAH) lectin family (beta-barrel scaffold); (3) the legume lectin-like lectin family (beta-sandwich scaffold); (4) the Galanthus nivalis agglutinin (GNA)-like lectin family (beta-prism II scaffold); and, (5) the MFP2-like lectin family (MFP2-like scaffold). Another algal lectin from Ulva pertusa, has been inferred to the methanol dehydrogenase related lectin family, because it displays a rather different GlcNAc-specificity. In spite of these structural discrepancies, all members from the five lectin families share a common ability to specifically recognize man-containing glycans and, especially, high-mannose type glycans. Because of their mannose-binding specificity, these lectins have been used as valuable tools for deciphering and characterizing the complex mannose-containing glycans from the glycocalyx covering both normal and transformed cells, and as diagnostic tools and therapeutic drugs that specifically recognize the altered high-mannose N-glycans occurring at the surface of various cancer cells. In addition to these anti-cancer properties, man-specific seaweed lectins have been widely used as potent human immunodeficiency virus (HIV-1)-inactivating proteins, due to their capacity to specifically interact with the envelope glycoprotein gp120 and prevent the virion infectivity of HIV-1 towards the host CD4+ T-lymphocyte cells in vitro

Simian immunodeficiency virus infection in wild-caught chimpanzees from Cameroon

Simian immunodeficiency viruses (SIVcpz) infecting chimpanzees (Pan troglodytes) in west central Africa are the closest relatives to all major variants of human immunodeficiency virus type 1 ([HIV-1]; groups M, N and O), and have thus been implicated as the source of the human infections; however, information concerning the prevalence, geographic distribution, and subspecies association of SIVcpz still remains limited. In this study, we tested 71 wild-caught chimpanzees from Cameroon for evidence of SIVcpz infection. Thirty-nine of these were of the central subspecies (Pan troglodytes troglodytes), and 32 were of the Nigerian subspecies (Pan troglodytes vellerosus), as determined by mitochondrial DNA analysis. Serological analysis determined that one P. t. troglodytes ape (CAM13) harbored serum antibodies that cross-reacted strongly with HIV-1 antigens; all other apes were seronegative. To characterize the newly identified virus, 14 partially overlapping viral fragments were amplified from fecal virion RNA and concatenated to yield a complete SIVcpz genome (9,284 bp). Phylogenetic analyses revealed that SIVcpzCAM13 fell well within the radiation of the SIVcpzPtt group of viruses, as part of a clade including all other SIVcpzPtt strains as well as HIV-1 groups M and N. However, SIVcpzCAM13 clustered most closely with SIVcpzGAB1 from Gabon rather than with SIVcpzCAM3 and SIVcpzCAM5 from Cameroon, indicating the existence of divergent SIVcpzPtt lineages within the same geographic region. These data, together with evidence of recombination among ancestral SIVcpzPtt lineages, indicate long-standing endemic infection of central chimpanzees and reaffirm a west central African origin of HIV-1. Whether P. t. vellerosus apes are naturally infected with SIVcpz requires further study

Essais d’introduction de sucro-glycérides dans les aliments d’allaitement pour veaux de boucherie. 1. — Effets du sucro-glycéride de palme, utilisé seul ou en association avec des antibiotiques.

Joussellin Wilfrid, Ladrat J., Craplet Camille, Barre P., Thoret J. P. Essais d’introduction de sucro-glycérides dans les aliments d’allaitement pour veaux de boucherie. I. Effets du sucro-glycéride de palme, utilisé seul ou en association avec des antibiotiques. In: Bulletin de l'Académie Vétérinaire de France tome 117 n°6, 1964. pp. 277-281

Interest of pet imaging in multiple myeloma

The interest of 18Fluoro-deoxyglucose (FDG) positron emission tomography (PET) imaging in the management of patients with multiple myeloma (MM) for the workup at diagnosis and for therapeutic evaluation has recently been demonstrated. FDG-PET is a powerful imaging tool for bone lesions detection at initial diagnosis with high sensitivity and specificity values. The independent pejorative prognostic value on progression-free survival (PFS) and overall survival (OS) of baseline PET-derived parameters (presence of extra-medullary disease (EMD), number of focal bone lesions (FLs), and maximum standardized uptake values [SUVmax]) has been reported in several large independent prospective studies. During therapeutic evaluation, FDG-PET is considered as the reference imaging technique, because it can be performed much earlier than MRI which lacks specificity. Persistence of significant FDG uptake after treatment, notably before maintenance therapy, is an independent pejorative prognostic factor, especially for patients with a complete biological response. So FDG-PET and medullary flow cytometry are complementary tools for detection of minimal residual disease before maintenance therapy. However, the definition of PET metabolic complete response should be standardized. In patients with smoldering multiple myeloma, the presence of at least one hyper-metabolic lytic lesions on FDG-PET may be considered as a criterion for initiating therapy. FDG-PET is also indicated for initial staging of a solitary plasmacytoma so as to not disregard other bone or extra-medullary localizations. Development of nuclear medicine offer new perspectives for MM imaging. Recent PET tracers are willing to overcome limitations of FDG. (11)C-Methionine, which uptake reflects the increased protein synthesis of malignant cells seems to correlate well with bone marrow infiltration. Lipid tracers, such as Choline or acetate, and some peptide tracers, such as (68) Ga-Pentixafor, that targets CXCR4 (chemokine receptor-4, which is often expressed with high density by myeloma cells), are other promising PET ligands. 18F-fludarabine and immuno-PET targeting CD138 and CD38 also showed promising results in preclinical models