The Macrocosm in the Microcosm of Folk Art

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Water quality between the Barron River/Trinity Inlet and Green Island and the effect of seasonal change

Nutrients and related water quality characteristics were measured at six sites along a transect from the Barron River estuary to Green Island over a twenty-month period. The water column was generally well mixed and so depth was not a significant source of variation. Overall, slightly higher nutrient concentrations tended to be found at the two most inshore sites. FUrthermore, some elevated nutrient levels were recorded right across the transect after periods of heavy rain and/or rough weather. This elevation was most noticeable at the two inshore sites, suggesting input from the Barron River discharge.· At Green Island there was no significant increase over background levels in the mean levels of nutrients in the vicinity of the sewage outfall, however relatively high chlorophyll a levels were recorded near to the sewage discharge. These high chlorophyll a levels could indeed result from the discharge of sewage effluent but further studies will need to be carried out in order to confirm this

Transcriptional factor influence on OTA production and the quelling attribute of Sirna on the OTA producing strains of Aspergillus section Nigri

This study determined the influence of some transcriptional factors on ochratoxin A production as well as investigates the quelling attributes of some designed siRNA on the OTA producing Aspergillus section Nigri using standard recommended techniques. Results obtained following comparison of the pks gene promoter sequences from 15 isolates depicts differences in length and homology with the pks gene ranging from 218bp in a strain of the Aspergillus niger to 700bp in Aspergillus carbonarius. The alignment of the pks gene promoter region revealed that six and two of the aligned genes have Aba A binding site corresponding to CATTCT and CATTCC respectively while Brl A binding site was absent in all the isolates. Pac C binding site corresponding to CCTGGC and GCCAAG was also found in two and three of the pks gene promoter region respectively. The three designed siRNA shows significant impact on OTA inhibitions with no significant statistical differences (80.9, 74.4 and 75.3% for pks_Ia, pks_Ib and pks_Ic respectively) (F= 3.830, p>0.05). It can be concluded that Are A and Aba A are potential enhancers for ochratoxin A biosynthesis and none of the investigated transcriptional factors is enough for the activation of ochratoxin A production. However, pks gene was seen as a good target gene for inactivation in order to develop efficient means for ochratoxin A control using RNA silencing technology.Key words: Transcriptional factors, Ochratoxin A, siRNA, Quelling , Aspergillus section Nigr

Genetic diversity of ochratoxigenic Aspergillus section Nigri, using RAPD and VCG techniques

This study evaluates the genetic diversity of ochratoxigenic Aspergillus section Nigri using RAPD and VCG techniques. Results obtained revealed OPX 07 as the most informative of the tested RAPD markers generating 12 polymorphic bands while the least bands were generated by OPR 19. Of the 40 Aspergillus section Nigri (20 each of Aspergillus niger and Aspergillus carbonarius), 22 VCGs and 27 RAPD haplotypes were delineated. The two techniques demonstrated similar resolution except in few cases where the RAPD technique further sub divided some VCGs into simpler haplotypes. The average percentage of variable VCG and RAPD reactions were 25 and 50% in that order of sequence while 75 and 50% of the isolates were resolved as same isolates by these techniques respectively. It was also found that the Simpson index of genetic diversity approached one for the isolates from the four geopolitical zones of Ogun State, Nigeria with the mean genetic diversity within isolates (GL) contributing significantly approximately 89% of the total diversity observed within the isolates (F=22.23, p<0.05). The remaining 11% of variation could only be allotted to diversity among isolates (GS). On the whole, the total genetic diversity (HT) was found to be approximately 48%. In conclusion, RAPD markers provided better resolution than the classical VCG typing technique.Keywords; Genetic Diversity, Ochratoxigenic Aspergillus, RAPD and VC

Improved brain PET quantification using partial volume correction techniques

Positron emission tomography (PET) suffers from a degradation in quantitative accuracy due to a phenomenon known as the partial volume effect (PVE). The effects are due to the limited spatial resolution of the scanner. Methods that correct for PVEs are known as partial volume correction (PVC) techniques and are either data-driven or make use of anatomical information from other modalities such as magnetic resonance (MR) imaging. This thesis reports investigations into PVC techniques for improving the quantification of brain amyloid PET tracers. These tracers image amyloid plaque aggregation in-vivo, which is a pathological hallmark of Alzheimer’s disease. An extension to existing anatomy-based PVC methods is reported. Region-based voxelwise (RBV) correction has been shown to reduce PVE-induced regional bias and variance when compared to commonly applied PVC techniques. This has been proven in phantom studies and observed in clinical data. In addition, RBV has been used to demonstrate that white matter variability exists in two different amyloid tracers. This finding has implications for the application of PVC in amyloid imaging and also how scans should be normalised. Alternative reference regions were investigated in two amyloid PET tracers. The brain stem, in combination with PVC, was found to result in the strongest agreement between tracers. Anatomy-based PVC techniques rely on parcellations of structural images. These parcellations are not necessarily representative of the PET data. A further extension to RBV is proposed which iteratively modifies the parcellations to find an optimal PVC in terms of the observed PET data. This novel technique reduces quantification errors due to PET-MR mismatch and has the potential to provide an additional parameter of ‘functional volume change’ in longitudinal studies

The Exact MSSM Spectrum from String Theory

We show the existence of realistic vacua in string theory whose observable sector has exactly the matter content of the MSSM. This is achieved by compactifying the E_8 x E_8 heterotic superstring on a smooth Calabi-Yau threefold with an SU(4) gauge instanton and a Z_3 x Z_3 Wilson line. Specifically, the observable sector is N=1 supersymmetric with gauge group SU(3)_C x SU(2)_L x U(1)_Y x U(1)_{B-L}, three families of quarks and leptons, each family with a right-handed neutrino, and one Higgs-Higgs conjugate pair. Importantly, there are no extra vector-like pairs and no exotic matter in the zero mode spectrum. There are, in addition, 6 geometric moduli and 13 gauge instanton moduli in the observable sector. The holomorphic SU(4) vector bundle of the observable sector is slope-stable.Comment: 15 pages, LaTeX; v2: Hidden sector is unstable, symbol typesetting error corrected, clarifications and references added; v3: New discussion of hidden secto

Identification and utilization of informative EST-SSR markers for genetic purity testing of coconut hybrids

Coconut palms are categorized into two forms, viz., ‘talls’ and ‘dwarfs’ which are being utilized to produce hybrids through the process of inter-varietal or intra-varietal crosses. Hybrid coconut seedlings are generally identified and selected based on morphological traits by plant breeders, which is quite difficult and requires expertise. Even minor errors in identification may adversely affect breeding programs in coconut, which is spread over many decades. In this study, we have utilized thirty EST-SSR markers, derived from existing coconut leaf transcriptome data, for screening polymorphism between eighteen coconut parental lines. The polymorphic primers capable of differentiating the parental palms were then utilized successfully for assessment of purity of hybrids derived from these parents. Thus, the current study demonstrates the utility of EST-SSR markers in determiningthe genetic purity of hybrids in coconut

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A sticky business: the status of the conjectured viscosity/entropy density bound

There have been a number of forms of a conjecture that there is a universal lower bound on the ratio, eta/s, of the shear viscosity, eta, to entropy density, s, with several different domains of validity. We examine the various forms of the conjecture. We argue that a number of variants of the conjecture are not viable due to the existence of theoretically consistent counterexamples. We also note that much of the evidence in favor of a bound does not apply to the variants which have not yet been ruled out.Comment: 23 pages, 4 figures, added references, corrected typos, added subsection in response to Son's comments in arXiv:0709.465

Applications of Solid Freeform Fabrication at the Naval Research Laboratory

Solid Freeform Fabrication (SFF) and related techniques are used at the Naval Research Laboratory (NRL) for a variety of materials related investigations. Research and applications conducted over the past few years are described including: Helisys Laminated Object Manufacturing System (LOMS) fabrication of: ceramic piezoelectric actuators, tooling for multifunctional materials, and anatomical prototypes for surgical visualization; fabrication of mesoscale electronic and sensor components using a laser forward transfer direct write technique; and visualization of complex, 3-D microstructures using a Stratasys Fused-Deposition Modeler. The paper closes with a brief overview of future SFF related work at the NRL.Support for this work from DARPA, Office of Naval Research, and the Naval Research Laboratory Core Research Program is gratefully acknowledged.Mechanical Engineerin