The National Health Accounts of the Philippines: Continuing Development and New Findings

For more than a decade now, the national health accounts (NHA) of the Philippines has been providing data that are important for health policymaking. At present, the NHA not only continues to be important for health policymaking. It has also become indispensable as a tool for monitoring and tracking outcomes of health sector policies. To further expand its usefulness, the Philippine NHA underwent major restructuring, specifically in the classification of health expenditures by uses of funds. The revised NHA now includes several types of breakdown by uses of funds. Analysis of preliminary 2003 estimates of the revised NHA matrices reveals new details of the manner by which health funds are utilized in the Philippines--data that are not available in the original NHA.health sector, national health accounts, health funds

The National Health Accounts of the Philippines: Continuing Development and New Findings

For more than a decade now, the national health accounts (NHA) of the Philippines has been providing data that are important for health policymaking. At present, the NHA not only continues to be important for health policymaking. It has also become indispensable as a tool for monitoring and tracking outcomes of health sector policies. To further expand its usefulness, the Philippine NHA underwent major restructuring, specifically in the classification of health expenditures by uses of funds. The revised NHA now includes several types of breakdown by uses of funds. Analysis of preliminary 2003 estimates of the revised NHA matrices reveals new details of the manner by which health funds are utilized in the Philippines--data that are not available in the original NHA.health sector, national health accounts, health funds

The next-to-leading order forward jet vertex in the small-cone approximation

We consider within QCD collinear factorization the process p+p to jet + jet +X, where two forward high-$p_T$ jets are produced with a large separation in rapidity $\Delta y$ (Mueller-Navelet jets). In this case the (calculable) hard part of the reaction receives large higher-order corrections $\sim \alpha^n_s (\Delta y)^n$, which can be accounted for in the BFKL approach. In particular, we calculate in the next-to-leading order the impact factor (vertex) for the production of a forward high-$p_T$ jet, in the approximation of small aperture of the jet cone in the pseudorapidity-azimuthal angle plane. The final expression for the vertex turns out to be simple and easy to implement in numerical calculations.Comment: 32 pages, 4 figures; a few comments and one reference added; a few inessential misprints removed; version to appear on JHE

The Effect of Compositional Context on Synthetic Gene Networks

It is well known that synthetic gene expression is highly sensitive to how genetic elements (promoter structure, spacing regions between promoter and coding sequences, ribosome binding sites, etc.) are spatially configured. An important topic that has received far less attention is how the compositional context, or spatial arrangement, of entire genes within a synthetic gene network affects their individual expression levels. In this paper we show, both quantitatively and qualitatively, that compositional context significantly alters transcription levels in synthetic gene networks. We demonstrate that key characteristics of gene induction, such as ultra-sensitivity and dynamic range, strongly depend on compositional context. We postulate that supercoiling can be used to explain this interference and validate this hypothesis through modeling and a series of in vitro supercoiling relaxation experiments. This compositional interference enables a novel form of feedback in synthetic gene networks. We illustrate the use of this feedback by redesigning the toggle switch to incorporate compositional context. We show the context-optimized toggle switch has improved threshold detection and memory properties

Crude incidence in two-phase designs in the presence of competing risks.

BackgroundIn many studies, some information might not be available for the whole cohort, some covariates, or even the outcome, might be ascertained in selected subsamples. These studies are part of a broad category termed two-phase studies. Common examples include the nested case-control and the case-cohort designs. For two-phase studies, appropriate weighted survival estimates have been derived; however, no estimator of cumulative incidence accounting for competing events has been proposed. This is relevant in the presence of multiple types of events, where estimation of event type specific quantities are needed for evaluating outcome.MethodsWe develop a non parametric estimator of the cumulative incidence function of events accounting for possible competing events. It handles a general sampling design by weights derived from the sampling probabilities. The variance is derived from the influence function of the subdistribution hazard.ResultsThe proposed method shows good performance in simulations. It is applied to estimate the crude incidence of relapse in childhood acute lymphoblastic leukemia in groups defined by a genotype not available for everyone in a cohort of nearly 2000 patients, where death due to toxicity acted as a competing event. In a second example the aim was to estimate engagement in care of a cohort of HIV patients in resource limited setting, where for some patients the outcome itself was missing due to lost to follow-up. A sampling based approach was used to identify outcome in a subsample of lost patients and to obtain a valid estimate of connection to care.ConclusionsA valid estimator for cumulative incidence of events accounting for competing risks under a general sampling design from an infinite target population is derived

Decadal changes of the Western Arabian sea ecosystem

Historical data from oceanographic expeditions and remotely sensed data on outgoing longwave radiation, temperature, wind speed and ocean color in the western Arabian Sea (1950–2010) were used to investigate decadal trends in the physical and biochemical properties of the upper 300 m. 72 % of the 29,043 vertical profiles retrieved originated from USA and UK expeditions. Increasing outgoing longwave radiation, surface air temperatures and sea surface temperature were identified on decadal timescales. These were well correlated with decreasing wind speeds associated with a reduced Siberian High atmospheric anomaly. Shoaling of the oxycline and nitracline was observed as well as acidification of the upper 300 m. These physical and chemical changes were accompanied by declining chlorophyll-a concentrations, vertical macrofaunal habitat compression, declining sardine landings and an increase of fish kill incidents along the Omani coast

On relativization of the Sommerfeld-Gamow-Sakharov factor

The Sommerfeld-Gamow-Sakharov factor is considered for the general case of arbitrary masses and energies. It is shown that the scalar triangular one-loop diagram gives the Coulomb singularity in radiative corrections at the threshold. The singular part of the correction is factorized at the complete Born cross section regardless of its partial wave decomposition. Different approaches to generalize the factor are discussed.Comment: 9 pages, 4 figures; references and discussion are extende

Electrostatically gated membrane permeability in inorganic protocells

Although several strategies are now available to produce functional microcompartments analogous to primitive cell-like structures, little progress has been made in generating protocell constructs with self-controlled membrane permeability. Here we describe the preparation of water-dispersible colloidosomes based on silica nanoparticles and delineated by a continuous semipermeable inorganic membrane capable of self-activated, electrostatically gated permeability. We use crosslinking and covalent grafting of a pH-responsive copolymer to generate an ultrathin elastic membrane that exhibits selective release and uptake of small molecules. This behaviour, which depends on the charge of the copolymer coronal layer, serves to trigger enzymatic dephosphorylation reactions specifically within the protocell aqueous interior. This system represents a step towards the design and construction of alternative types of artificial chemical cells and protocell models based on spontaneous processes of inorganic self-organization

Modulation of enhancer looping and differential gene targeting by Epstein-Barr virus transcription factors directs cellular reprogramming

Epstein-Barr virus (EBV) epigenetically reprogrammes B-lymphocytes to drive immortalization and facilitate viral persistence. Host-cell transcription is perturbed principally through the actions of EBV EBNA 2, 3A, 3B and 3C, with cellular genes deregulated by specific combinations of these EBNAs through unknown mechanisms. Comparing human genome binding by these viral transcription factors, we discovered that 25% of binding sites were shared by EBNA 2 and the EBNA 3s and were located predominantly in enhancers. Moreover, 80% of potential EBNA 3A, 3B or 3C target genes were also targeted by EBNA 2, implicating extensive interplay between EBNA 2 and 3 proteins in cellular reprogramming. Investigating shared enhancer sites neighbouring two new targets (WEE1 and CTBP2) we discovered that EBNA 3 proteins repress transcription by modulating enhancer-promoter loop formation to establish repressive chromatin hubs or prevent assembly of active hubs. Re-ChIP analysis revealed that EBNA 2 and 3 proteins do not bind simultaneously at shared sites but compete for binding thereby modulating enhancer-promoter interactions. At an EBNA 3-only intergenic enhancer site between ADAM28 and ADAMDEC1 EBNA 3C was also able to independently direct epigenetic repression of both genes through enhancer-promoter looping. Significantly, studying shared or unique EBNA 3 binding sites at WEE1, CTBP2, ITGAL (LFA-1 alpha chain), BCL2L11 (Bim) and the ADAMs, we also discovered that different sets of EBNA 3 proteins bind regulatory elements in a gene and cell-type specific manner. Binding profiles correlated with the effects of individual EBNA 3 proteins on the expression of these genes, providing a molecular basis for the targeting of different sets of cellular genes by the EBNA 3s. Our results therefore highlight the influence of the genomic and cellular context in determining the specificity of gene deregulation by EBV and provide a paradigm for host-cell reprogramming through modulation of enhancer-promoter interactions by viral transcription factors