Neuropeptide Degradation Produces Functional Inactivation in the Crustacean Nervous System

The pentapeptide proctolin (Proct.; Arg-Tyr-Leu-Pro-Thr) is a modulatory transmitter found throughout the crustacean nervous system. No information is available in this system, however, as to how the actions of this peptide are terminated. To study this issue in the crab Cancer borealis, we incubated exogenous proctolin (10(-5) M) with either the thoracic ganglion (TG) or with conditioned saline (CS) that had been preincubated with the TG. We removed aliquots at standard time points for analysis by reverse-phase high-performance liquid chromatography (HPLC). We found that over time the proctolin peak became progressively smaller, while three novel peaks appeared and increased in size. Comigration experiments using HPLC indicated that the major novel peak was Proct. (Tyr-Leu-Pro-Thr), while one of the two minor peaks was Proct. (Leu-Pro-Thr). The other minor peak appeared to be Proct. (Arg-Tyr), based on similar HPLC retention time to synthetic Proct. The reduction in the proctolin peak and the increase in the Proct. peak was prevented by co-incubation of proctolin with any one of several aminopeptidase inhibitors (10(-4) M). Proct. and Proct. appeared to result from a diaminopeptidase-mediated cleavage of proctolin. We tested whether N-terminal cleavage functionally inactivated proctolin by coapplying proctolin (10(-8) M) and individual aminopeptidase inhibitors (10(-5) M) to the isolated stomatogastric ganglion (STG). We found that these inhibitors significantly enhanced the proctolin excitation of the pyloric rhythm. Furthermore, application of synthetic Proct. to the STG had no effect unless high concentrations (\u3e 10(-6) M) were used, and neither Proct. nor Proct. (10(-4) M) influenced the pyloric rhythm. Our results indicate that proctolin is enzymatically degraded and thereby biologically inactivated in the crab nervous system, primarily by extracellularly located aminopeptidase activity

An architecture for personalized systems based on web mining agents

[EN]The development of the present web systems is becoming a complex activity due to the need to integrate the last technologies in order to make more efficient and competitive applications. Endowing systems with personalized recommendation procedures contributes to achieve these objectives. In this paper, a web mining method for personalization is proposed. It uses the information already available from other users to discover patterns that are used later for making recommendations. The work deals with the problem of introducing new information items and new users who do not have a profile. We propose an architectural design of intelligent data mining agents for the system implementation

Conservation laws and scattering for de Sitter classical particles

Starting from an intrinsic geometric characterization of de Sitter timelike and lightlike geodesics we give a new description of the conserved quantities associated with classical free particles on the de Sitter manifold. These quantities allow for a natural discussion of classical pointlike scattering and decay processes. We also provide an intrinsic definition of energy of a classical de Sitter particle and discuss its different expressions in various local coordinate systems and their relations with earlier definitions found in the literature.Comment: 25 pages, 1 figur

Fibre bundle formulation of nonrelativistic quantum mechanics: I. Introduction. The evolution transport

We propose a new systematic fibre bundle formulation of nonrelativistic quantum mechanics. The new form of the theory is equivalent to the usual one but it is in harmony with the modern trends in theoretical physics and potentially admits new generalizations in different directions. In it a pure state of some quantum system is described by a state section (along paths) of a (Hilbert) fibre bundle. Its evolution is determined through the bundle (analogue of the) Schr\"odinger equation. Now the dynamical variables and the density operator are described via bundle morphisms (along paths). The mentioned quantities are connected by a number of relations derived in this work. The present first part of this investigation is devoted to the introduction of basic concepts on which the fibre bundle approach to quantum mechanics rests. We show that the evolution of pure quantum-mechanical states can be described as a suitable linear transport along paths, called evolution transport, of the state sections in the Hilbert fibre bundle of states of a considered quantum system.Comment: 26 standard (11pt, A4) LaTeX 2e pages. The packages AMS-LaTeX and amsfonts are required. Revised: new material, references, and comments are added. Minor style chages. Continuation of quan-ph/9803083. For continuation of the this series see http://www.inrne.bas.bg/mathmod/bozhome

Schr\"{o}dinger Fields on the Plane with [U(1)]N[U(1)]^N Chern-Simons Interactions and Generalized Self-dual Solitons

A general non-relativistic field theory on the plane with couplings to an arbitrary number of abelian Chern-Simons gauge fields is considered. Elementary excitations of the system are shown to exhibit fractional and mutual statistics. We identify the self-dual systems for which certain classical and quantal aspects of the theory can be studied in a much simplified mathematical setting. Then, specializing to the general self-dual system with two Chern-Simons gauge fields (and non-vanishing mutual statistics parameter), we present a systematic analysis for the static vortexlike classical solutions, with or without uniform background magnetic field. Relativistic generalizations are also discussed briefly.Comment: 49 pages including 4 figures, LATEX ( three LATEX figures and one PICTEX figure), SNUTP 93-14, UMN-TH-113

To conserve African tropical forests, invest in the protection of its most endangered group of monkeys, red colobus

Forest loss and overhunting are eroding African tropical biodiversity and threatening local human food security, livelihoods, and health. Emblematic of this ecological crisis is Africa's most endangered group of monkeys, the red colobus (genus Piliocolobus). All 17 species, found in forests from Senegal in the west to the Zanzibar archipelago in the east, are threatened with extinction. Red colobus are among the most vulnerable mammals to gun hunting, typically disappearing from heavily hunted forests before most other large-bodied animals. Despite their conservation status, they are rarely a focus of conservation attention and continue to be understudied. However, red colobus can act as critical barometers of forest health and serve as flagships for catalyzing broader African tropical forest conservation efforts. We offer a plan for conservation of red colobus and their habitats and discuss conservation and policy implications.Additional authors: Deo Kujirakwinja, Barney Long, W. Scott McGraw, Russell A. Mittermeier, Thomas T. Struhsake