Establishment of a quantitative real-time PCR assay for the specific quantification of Ca. Phytoplasma prunorum in plants and insects

A real-time PCR assay for the quantification of Ca. Phytoplasma prunorum has been established which combines the specificity of detection with a low cost method of quantitative PCR. The assay uses the specific primers ECA1/ECA2 with a SYBR Green I protocol. A gene fragment of Ca. P. prunorum with the target of the primers has been cloned and is used as standard for quantification by the standard curve method. The assay has been successfully applied to measure the concentration of Ca. P. prunorum in insects as well as in different kinds of plant samples. Keywords: European stone fruit yellows, Cacopsylla pruni, resistance screenin

Analysis of the acquisition and multiplication efficiency of different strains of Ca. Phytoplasma mali by the vector Cacopsylla picta

Based on previous observations during long-term acquisition and transmission trials, studies were carried out under standardized conditions in order to analyse the acquisition and multiplication efficiencies of different strains of Candidatus Phytoplasma mali by different developmental stages of Cacopsylla picta. The acquisition of Ca. P. mali from micropropagated plants infected with different strains was tested for nymphs, larval stages and new adults of C. picta. When born on infected plants a nearly 100% acquisition was achieved for all strains of Ca. P. mali by C. picta. Differences in acquisition efficiency were observed for new generation adults which acquired the phytoplasma as imagines. The multiplication efficiency of the different Ca. P. mali strains inside the insects was analysed by quantitative real-time PCR. Significant differences in the capacity of the different strains to colonise the insect were found. Despite high acquisition rates only few subsequent transmission events to healthy test plants could be recorded

Comparative evaluation of insecticide efficacy tests against Drosophila suzukii on grape berries in laboratory, semi-field and field trials

As the period for field trials on grapevine is limited, we designed a laboratory test system to evaluate the effectiveness of selected insecticides against spotted-wing Drosophila (SWD), Drosophila suzukii, on different types of grape berries all year round. Tests were undertaken during winter and early spring with table grapes of different purchased varieties according to their seasonal availability and with wine grapes from experimental field plots in autumn. In preliminary experiments, we defined parameters for a standard laboratory test system for screening the effectiveness of several formulated insecticides in two different experimental set-ups: i) application before confining adults with berries and ii) application after confining adult D. suzukii with berries. These approaches allowed us to determine the contact activity of the products on adult D. suzukii or the impact on the larval development until the emergence of adult flies. The developed test system is suitable for screening substances with diverse types of activity on different grape types. In a second step, we combined laboratory bioassays with field applications in a semi-field persistence study and lastly we installed a randomized field plot in order to compare the effectiveness of selected insecticides in the laboratory and under field conditions. In all cases, the products Karate Zeon and SpinTor proved most efficacious in their contact mortality or as oviposition deterrents, while Mospilan SG and Coragen exhibited a good larvicidal activity. However, important disagreements occurred for the efficacy of currently authorized insecticides among laboratory, semi-field and practical field applications. The transferability of laboratory results into the field is discussed

When a Palearctic bacterium meets a Nearctic insect vector: Genetic and ecological insights into the emergence of the grapevine Flavescence dorée epidemics in Europe

Flavescence dorée (FD) is a European quarantine grapevine disease transmitted by the Deltocephalinae leafhopper Scaphoideus titanus. Whereas this vector had been introduced from North America, the possible European origin of FD phytoplasma needed to be challenged and correlated with ecological and genetic drivers of FD emergence. For that purpose, a survey of genetic diversity of these phytoplasmas in grapevines, S. titanus, black alders, alder leafhoppers and clematis were conducted in five European countries. Out of 132 map genotypes, only 11 were associated to FD outbreaks, three were detected in clematis, whereas 127 were detected in alder trees, alder leafhoppers or in grapevines out of FD outbreaks. Most of the alder trees were found infected, including 8% with FD genotypes M6, M38 and M50, also present in alders neighboring FD-free vineyards and vineyard-free areas. The Macropsinae Oncopsis alni could transmit genotypes unable to achieve transmission by S. titanus, while the Deltocephalinae Allygus spp. and Orientus ishidae transmitted M38 and M50 that proved to be compatible with S. titanus. Variability of vmpA and vmpB adhesin-like genes clearly discriminated 3 genetic clusters. Cluster Vmp-I grouped genotypes only transmitted by O. alni, while clusters Vmp-II and -III grouped genotypes transmitted by Deltocephalinae leafhoppers. Interestingly, adhesin repeated domains evolved independently in cluster Vmp-I, whereas in clusters Vmp-II and-III showed recent duplications. Latex beads coated with various ratio of VmpA of clusters II and I, showed that cluster II VmpA promoted enhanced adhesion to the Deltocephalinae Euscelidius variegatus epithelial cells and were better retained in both E. variegatus and S. titanus midguts. Our data demonstrate that most FD phytoplasmas are endemic to European alders. Their emergence as grapevine epidemic pathogens appeared restricted to some genetic variants pre-existing in alders, whose compatibility to S. titanus correlates with different vmp gene sequences and VmpA binding properties

Collaboration and knowledge exchange between scholars in Britain and the empire, 1830–1914

In recent years there has been a growing interest among historians in the British Empire as a space of knowledge production and circulation. Much of this work assumes that scholarly cooperation and collaboration between individuals and institutions within the Empire had the effect (and often also the aim) of strengthening both imperial ties and the idea of empire. This chapter argues, however, that many examples of scholarly travel, exchange, and collaboration were undertaken with very different goals in mind. In particular, it highlights the continuing importance of an ideal of scientific internationalism, which stressed the benefits of scholarship for the whole of humanity and prioritized the needs and goals of individual academic and scientific disciplines. As the chapter shows, some scholars even went on to develop nuanced critiques of the imperial project while using the very structures of empire to further their own individual, disciplinary and institutional goals

Organizing Engineering Work - A Comparative-Analysis

This article analyzes the organization of engineering work in six industrial capitalist countries. It identifies four major models for the organization of engineering work; the engineering profession did not succeed in achieving professional “closure” in any of the six countries under review. A review of the historical evolution of the organization of engineering work in each of the six countries reveals that engineering has been shaped by a complex interaction among the profession itself, employers, the state, labor, and preindustrial forces. However, none of the national variations on the four models for organizing engineering labor is stable or without internal contradiction because of the ambiguous “intermediate” position of engineers

Effects of Crystalline Anisotropy and Indenter Size on Nanoindentation by Multiscale Simulation

Nanoindentation processes in single crystal Ag thin film under different crystallographic orientations and various indenter widths are simulated by the quasicontinuum method. The nanoindentation deformation processes under influences of crystalline anisotropy and indenter size are investigated about hardness, load distribution, critical load for first dislocation emission and strain energy under the indenter. The simulation results are compared with previous experimental results and Rice-Thomson (R-T) dislocation model solution. It is shown that entirely different dislocation activities are presented under the effect of crystalline anisotropy during nanoindentation. The sharp load drops in the load–displacement curves are caused by the different dislocation activities. Both crystalline anisotropy and indenter size are found to have distinct effect on hardness, contact stress distribution, critical load for first dislocation emission and strain energy under the indenter. The above quantities are decreased at the indenter into Ag thin film along the crystal orientation with more favorable slip directions that easy trigger slip systems; whereas those will increase at the indenter into Ag thin film along the crystal orientation with less or without favorable slip directions that hard trigger slip systems. The results are shown to be in good agreement with experimental results and R-T dislocation model solution